ID   P2C24_ARATH             Reviewed;         362 AA.
AC   Q9ZW21;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   27-MAR-2024, entry version 147.
DE   RecName: Full=Probable protein phosphatase 2C 24;
DE            Short=AtPP2C24;
DE            EC=3.1.3.16;
GN   OrderedLocusNames=At2g29380; ORFNames=F16P2.24;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA   Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL   Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=19021904; DOI=10.1186/1471-2164-9-550;
RA   Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.;
RT   "Genome-wide and expression analysis of protein phosphatase 2C in rice and
RT   Arabidopsis.";
RL   BMC Genomics 9:550-550(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + O-phospho-L-seryl-[protein] = L-seryl-[protein] +
CC         phosphate; Xref=Rhea:RHEA:20629, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC         COMP:11604, ChEBI:CHEBI:15377, ChEBI:CHEBI:29999, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:83421; EC=3.1.3.16;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] +
CC         phosphate; Xref=Rhea:RHEA:47004, Rhea:RHEA-COMP:11060, Rhea:RHEA-
CC         COMP:11605, ChEBI:CHEBI:15377, ChEBI:CHEBI:30013, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:61977; EC=3.1.3.16;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Note=Binds 2 magnesium or manganese ions per subunit. {ECO:0000250};
CC   -!- INTERACTION:
CC       Q9ZW21; Q9S7Z2: AFP4; NbExp=4; IntAct=EBI-4441103, EBI-1778843;
CC       Q9ZW21; A0SVK0: DOG1; NbExp=5; IntAct=EBI-4441103, EBI-25512274;
CC       Q9ZW21; O23160: MYB73; NbExp=3; IntAct=EBI-4441103, EBI-25506855;
CC       Q9ZW21; Q8H1R0: PYL10; NbExp=7; IntAct=EBI-4441103, EBI-2363213;
CC       Q9ZW21; Q9FJ50: PYL11; NbExp=7; IntAct=EBI-4441103, EBI-2363233;
CC       Q9ZW21; Q9FJ49: PYL12; NbExp=3; IntAct=EBI-4441103, EBI-2363244;
CC       Q9ZW21; Q9SN51: PYL13; NbExp=3; IntAct=EBI-4441103, EBI-25515027;
CC       Q9ZW21; Q9SSM7: PYL3; NbExp=3; IntAct=EBI-4441103, EBI-2363144;
CC       Q9ZW21; O80920: PYL4; NbExp=3; IntAct=EBI-4441103, EBI-2349683;
CC       Q9ZW21; Q9FLB1: PYL5; NbExp=3; IntAct=EBI-4441103, EBI-2363181;
CC       Q9ZW21; Q8S8E3: PYL6; NbExp=3; IntAct=EBI-4441103, EBI-2363192;
CC       Q9ZW21; Q1ECF1: PYL7; NbExp=5; IntAct=EBI-4441103, EBI-2363203;
CC       Q9ZW21; Q9FGM1: PYL8; NbExp=7; IntAct=EBI-4441103, EBI-2429535;
CC       Q9ZW21; Q84MC7: PYL9; NbExp=8; IntAct=EBI-4441103, EBI-2349513;
CC   -!- SIMILARITY: Belongs to the PP2C family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AC004561; AAC95200.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC08244.1; -; Genomic_DNA.
DR   EMBL; DQ056553; AAY78704.1; -; mRNA.
DR   EMBL; BT022047; AAY25459.1; -; mRNA.
DR   EMBL; BT023483; AAY57322.1; -; mRNA.
DR   PIR; F84695; F84695.
DR   RefSeq; NP_180499.1; NM_128492.3.
DR   AlphaFoldDB; Q9ZW21; -.
DR   SMR; Q9ZW21; -.
DR   BioGRID; 2837; 15.
DR   DIP; DIP-48991N; -.
DR   IntAct; Q9ZW21; 18.
DR   STRING; 3702.Q9ZW21; -.
DR   PaxDb; 3702-AT2G29380-1; -.
DR   EnsemblPlants; AT2G29380.1; AT2G29380.1; AT2G29380.
DR   GeneID; 817487; -.
DR   Gramene; AT2G29380.1; AT2G29380.1; AT2G29380.
DR   KEGG; ath:AT2G29380; -.
DR   Araport; AT2G29380; -.
DR   TAIR; AT2G29380; HAI3.
DR   eggNOG; KOG0698; Eukaryota.
DR   HOGENOM; CLU_013173_20_0_1; -.
DR   InParanoid; Q9ZW21; -.
DR   OMA; SWTETME; -.
DR   OrthoDB; 415067at2759; -.
DR   PhylomeDB; Q9ZW21; -.
DR   PRO; PR:Q9ZW21; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; Q9ZW21; baseline and differential.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0017018; F:myosin phosphatase activity; IEA:UniProtKB-EC.
DR   CDD; cd00143; PP2Cc; 1.
DR   Gene3D; 3.60.40.10; PPM-type phosphatase domain; 1.
DR   InterPro; IPR015655; PP2C.
DR   InterPro; IPR000222; PP2C_BS.
DR   InterPro; IPR036457; PPM-type-like_dom_sf.
DR   InterPro; IPR001932; PPM-type_phosphatase-like_dom.
DR   PANTHER; PTHR47992; PROTEIN PHOSPHATASE; 1.
DR   PANTHER; PTHR47992:SF40; PROTEIN PHOSPHATASE 2C 24-RELATED; 1.
DR   Pfam; PF00481; PP2C; 1.
DR   SMART; SM00332; PP2Cc; 1.
DR   SUPFAM; SSF81606; PP2C-like; 1.
DR   PROSITE; PS01032; PPM_1; 1.
DR   PROSITE; PS51746; PPM_2; 1.
DR   Genevisible; Q9ZW21; AT.
PE   1: Evidence at protein level;
KW   Hydrolase; Magnesium; Manganese; Metal-binding; Protein phosphatase;
KW   Reference proteome.
FT   CHAIN           1..362
FT                   /note="Probable protein phosphatase 2C 24"
FT                   /id="PRO_0000367953"
FT   DOMAIN          77..360
FT                   /note="PPM-type phosphatase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01082"
FT   BINDING         117
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         117
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         118
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         295
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         351
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   362 AA;  40297 MW;  D127B5057DA3E0D4 CRC64;
     MAEICYEVVT DACPSSVYES TPAHSRRRPR FQTVMHEDWE KNCKRSKQEA LATRYSSIPR
     SSREDFSDQN VDVSSPRYGV SSVCGRRREM EDAVAIHPSF SSPKNSEFPQ HYFGVYDGHG
     CSHVAARCRE RLHKLVQEEL SSDMEDEEEW KTTMERSFTR MDKEVVSWGD SVVTANCKCD
     LQTPACDSVG STAVVSVITP DKIVVANCGD SRAVLCRNGK PVPLSTDHKP DRPDELDRIE
     GAGGRVIYWD CPRVLGVLAM SRAIGDNYLK PYVSCEPEVT ITDRRDDDCL ILASDGLWDV
     VSNETACSVA RMCLRGGGRR QDNEDPAISD KACTEASVLL TKLALARNSS DNVSVVVIDL
     RR
//