Q9ZVX1 (UBC23_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable ubiquitin-conjugating enzyme E2 23
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein 23

Gene names

Name:	UBC23
Ordered Locus Names:	At2g16920
ORF Names:	F12A24.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	1102 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytosol Inferred from direct assay PubMed 21166475. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1102	1102	Probable ubiquitin-conjugating enzyme E2 23		PRO_0000345189
Regions
	Compositional bias	60 – 86	27	Asp-rich
	Compositional bias	262 – 265	4	Poly-Ser
	Compositional bias	658 – 661	4	Poly-Asp
	Compositional bias	747 – 750	4	Poly-Ser
Sites
	Active site	936	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZVX1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: DA2C11385355E8D9	FASTA	1,102	122,183
	10 20 30 40 50 60 MEHEQDDPGT STNVGVDSSV DDSMASLSIC DSEHPNIYRQ DIVKNKKTGS VGVVSEVAGD 70 80 90 100 110 120 SDSDSDISDE EEDDDDDEDN DDDDEDVEEG KKASEENVVN GDGEKKADGN YKCGALEGDQ 130 140 150 160 170 180 IRVLWMDNTE PVQDINDVTV IDRGFLHGDY VASASEPTGQ VGVVVDVNIS VDLLAPDGSI 190 200 210 220 230 240 HKDISTKNLK RVRDFAVGDY VVHGPWLGRI DDVLDNVTVL FDDGSMCKVL RVEPLRLKPI 250 260 270 280 290 300 PKNNLEEDAN FPYYPGQRVK ASSSSVFKNS RWLSGLWKPN RLEGTVTKVT AGSIFVYWIA 310 320 330 340 350 360 SAGFGPDSSV SPPEEQNPSN LTLLSCFTHA NWQVGDWCLL PSLNQSATIP LHKHVSKLRL 370 380 390 400 410 420 YDSQADRQQK IGRDLEDVQD EVSGKVEPAG ITAEALPKVT SDDPPQRNPS VSKEPVHEPW 430 440 450 460 470 480 PLHRKKIRKL VIRKDKKVKK KEESFEQALL VVNSRTRVDV SWQDGTIECR REAITLIPIE 490 500 510 520 530 540 TPGDHEFVSE QYVVEKTSDD GDNTTEPRRA GVVKNVNAKD RTASVRWLNP LRRAEEPREF 550 560 570 580 590 600 EKEEIVSVYE LEGHPDYDYC YGDVVVRLSP IAVALPASSP GNSFEEATQQ DNGYQDSESH 610 620 630 640 650 660 QEAKILVDKE ENEPSTDLSK LSWVGNITGL KDGDIEVTWA DGTISTVGPH AVYVVGRDDD 670 680 690 700 710 720 DESVAGESET SDAASWETLN DDDRGAPEIP EEDLGRSSSI EGNSDADIYA ENDSGRNGAL 730 740 750 760 770 780 ALPLAAIEFV TRLASGIFSR ARKSVDSSSS DYTVENVYKQ AESTNPSDET DSLDDPSPSK 790 800 810 820 830 840 VNVTDNCESK GTQANAKNIL SGETSTFLED EDKPVPSEGD SCSFRRFDIS QDPLDHHFLG 850 860 870 880 890 900 VDGQKTKERQ WFKKVDQDWK ILQNNLPDGI FVRAYEDRMD LLRAVIVGAF GTPYQDGLFF 910 920 930 940 950 960 FDFHLPSDYP SVPPSAYYHS GGWRLNPNLY EEGKVCLSLL NTWTGRGNEV WDPKSSSILQ 970 980 990 1000 1010 1020 VLVSLQGLVL NSKPYFNEAG YDKQVGTAEG EKNSLGYNEN TFLLNCKTMM YLMRKPPKDF 1030 1040 1050 1060 1070 1080 EELIKDHFRK RGYYILKACD AYMKGYLIGS LTKDASVIDE RSSANSTSVG FKLMLAKIAP 1090 1100 KLFSALSEVG ADCNEFQHLQ QQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC005167 Genomic DNA. Translation: AAC64223.1. CP002685 Genomic DNA. Translation: AEC06554.1.
IPI	IPI00536997.
PIR	H84545.
RefSeq	NP_179284.1. NM_127245.2.
UniGene	At.48482.
3D structure databases
HSSP	HSSP built from PDB template 2ESK based on UniProtKB P62837.
ProteinModelPortal	Q9ZVX1.
SMR	Q9ZVX1. Positions 870-1043.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT2G16920.1-P.
Proteomic databases
PaxDb	Q9ZVX1.
PRIDE	Q9ZVX1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G16920.1; AT2G16920.1; AT2G16920.
GeneID	816195.
KEGG	ath:AT2G16920.
Organism-specific databases
TAIR	At2g16920.
Phylogenomic databases
eggNOG	NOG238109.
HOGENOM	HOG000237457.
InParanoid	Q9ZVX1.
KO	K10581.
OMA	GHPDYDY.
PhylomeDB	Q9ZVX1.
ProtClustDB	CLSN2913237.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Genevestigator	Q9ZVX1.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. False negative. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBC23_ARATH

Accession

Primary (citable) accession number: Q9ZVX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program