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Protein

Glutathione S-transferase Z1

Gene

GSTZ1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts a maleylacetone isomerase. Also catalyzes the glutathione-dependent dehalogenation of dichloroacetic acid to glyoxylic acid. In vitro, possesses glutathione peroxidase activity toward cumene hydroperoxide and linoleic acid-13-hydroperoxide.1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei46GlutathioneBy similarity1
Binding sitei60Glutathione; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei112GlutathioneBy similarity1

GO - Molecular functioni

GO - Biological processi

  • aromatic amino acid family metabolic process Source: InterPro
  • glutathione metabolic process Source: GO_Central
  • homogentisate catabolic process Source: TAIR
  • toxin catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Oxidoreductase, Peroxidase, Transferase

Keywords - Biological processi

Detoxification, Stress response

Enzyme and pathway databases

BioCyciARA:AT2G02390-MONOMER.
BRENDAi5.2.1.2. 399.
ReactomeiR-ATH-156590. Glutathione conjugation.
R-ATH-71182. Phenylalanine and tyrosine catabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase Z1 (EC:2.5.1.18)
Short name:
AtGSTZ1
Alternative name(s):
GST class-zeta member 1
Glutathione S-transferase 18
Maleylacetone isomerase (EC:5.2.1.-)
Short name:
MAI
Gene namesi
Name:GSTZ1
Synonyms:GST18, GSTZ
Ordered Locus Names:At2g02390
ORF Names:T16F16.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G02390.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • cytosol Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001860291 – 221Glutathione S-transferase Z1Add BLAST221

Proteomic databases

PaxDbiQ9ZVQ3.

Expressioni

Inductioni

By salicylic acid, methyl jasmonate, auxin, H2O2, and the pathogen Hyaloperonospora parasitica.1 Publication

Gene expression databases

ExpressionAtlasiQ9ZVQ3. baseline and differential.
GenevisibleiQ9ZVQ3. AT.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi3702.AT2G02390.3.

Structurei

Secondary structure

1221
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 13Combined sources4
Helixi18 – 29Combined sources12
Beta strandi35 – 38Combined sources4
Turni41 – 44Combined sources4
Helixi45 – 47Combined sources3
Helixi49 – 54Combined sources6
Beta strandi60 – 65Combined sources6
Beta strandi68 – 72Combined sources5
Helixi73 – 83Combined sources11
Helixi94 – 109Combined sources16
Helixi138 – 151Combined sources14
Beta strandi156 – 158Combined sources3
Beta strandi161 – 163Combined sources3
Helixi166 – 182Combined sources17
Helixi190 – 199Combined sources10
Helixi203 – 208Combined sources6
Helixi210 – 212Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E6BX-ray1.65A1-221[»]
ProteinModelPortaliQ9ZVQ3.
SMRiQ9ZVQ3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9ZVQ3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 88GST N-terminalAdd BLAST82
Domaini93 – 218GST C-terminalAdd BLAST126

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni17 – 22Glutathione bindingBy similarity6
Regioni17 – 18Glutathione bindingBy similarity2
Regioni46 – 47Glutathione bindingBy similarity2
Regioni59 – 60Glutathione bindingBy similarity2
Regioni72 – 73Glutathione bindingBy similarity2
Regioni116 – 118Glutathione bindingBy similarity3

Sequence similaritiesi

Belongs to the GST superfamily. Zeta family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiKOG0868. Eukaryota.
COG0625. LUCA.
HOGENOMiHOG000125758.
InParanoidiQ9ZVQ3.
KOiK01800.
OMAiHQIASIV.
PhylomeDBiQ9ZVQ3.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR005955. Mal_ac_isom.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF13409. GST_N_2. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR01262. maiA. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9ZVQ3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MANSGEEKLK LYSYWRSSCA HRVRIALALK GLDYEYIPVN LLKGDQFDSD
60 70 80 90 100
FKKINPMGTV PALVDGDVVI NDSFAIIMYL DEKYPEPPLL PRDLHKRAVN
110 120 130 140 150
YQAMSIVLSG IQPHQNLAVI RYIEEKINVE EKTAWVNNAI TKGFTALEKL
160 170 180 190 200
LVNCAGKHAT GDEIYLADLF LAPQIHGAIN RFQINMEPYP TLAKCYESYN
210 220
ELPAFQNALP EKQPDAPSST I
Length:221
Mass (Da):24,888
Last modified:May 1, 1999 - v1
Checksum:i106BDC8EF3E745BF
GO
Isoform 2 (identifier: Q9ZVQ3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     49-49: S → SVYRFDLQ

Note: No experimental confirmation available.
Show »
Length:228
Mass (Da):25,810
Checksum:i80B56ED20594C9E8
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04193649S → SVYRFDLQ in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF288182 mRNA. Translation: AAG30131.1.
AJ278293 mRNA. Translation: CAC19475.1.
AY208155 mRNA. Translation: AAO60039.1.
AC005312 Genomic DNA. Translation: AAC78521.1.
CP002685 Genomic DNA. Translation: AEC05573.1.
CP002685 Genomic DNA. Translation: AEC05575.1.
AY052332 mRNA. Translation: AAK96525.1.
AY061901 mRNA. Translation: AAL31228.1.
AK226342 mRNA. Translation: BAE98491.1.
PIRiB84436.
RefSeqiNP_178344.1. NM_126296.5. [Q9ZVQ3-1]
NP_973400.1. NM_201671.3. [Q9ZVQ3-2]
UniGeneiAt.10192.

Genome annotation databases

EnsemblPlantsiAT2G02390.1; AT2G02390.1; AT2G02390. [Q9ZVQ3-1]
GeneIDi814770.
GrameneiAT2G02390.1; AT2G02390.1; AT2G02390.
KEGGiath:AT2G02390.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF288182 mRNA. Translation: AAG30131.1.
AJ278293 mRNA. Translation: CAC19475.1.
AY208155 mRNA. Translation: AAO60039.1.
AC005312 Genomic DNA. Translation: AAC78521.1.
CP002685 Genomic DNA. Translation: AEC05573.1.
CP002685 Genomic DNA. Translation: AEC05575.1.
AY052332 mRNA. Translation: AAK96525.1.
AY061901 mRNA. Translation: AAL31228.1.
AK226342 mRNA. Translation: BAE98491.1.
PIRiB84436.
RefSeqiNP_178344.1. NM_126296.5. [Q9ZVQ3-1]
NP_973400.1. NM_201671.3. [Q9ZVQ3-2]
UniGeneiAt.10192.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E6BX-ray1.65A1-221[»]
ProteinModelPortaliQ9ZVQ3.
SMRiQ9ZVQ3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G02390.3.

Proteomic databases

PaxDbiQ9ZVQ3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G02390.1; AT2G02390.1; AT2G02390. [Q9ZVQ3-1]
GeneIDi814770.
GrameneiAT2G02390.1; AT2G02390.1; AT2G02390.
KEGGiath:AT2G02390.

Organism-specific databases

TAIRiAT2G02390.

Phylogenomic databases

eggNOGiKOG0868. Eukaryota.
COG0625. LUCA.
HOGENOMiHOG000125758.
InParanoidiQ9ZVQ3.
KOiK01800.
OMAiHQIASIV.
PhylomeDBiQ9ZVQ3.

Enzyme and pathway databases

BioCyciARA:AT2G02390-MONOMER.
BRENDAi5.2.1.2. 399.
ReactomeiR-ATH-156590. Glutathione conjugation.
R-ATH-71182. Phenylalanine and tyrosine catabolism.

Miscellaneous databases

EvolutionaryTraceiQ9ZVQ3.
PROiQ9ZVQ3.

Gene expression databases

ExpressionAtlasiQ9ZVQ3. baseline and differential.
GenevisibleiQ9ZVQ3. AT.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR005955. Mal_ac_isom.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF13409. GST_N_2. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR01262. maiA. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSTZ1_ARATH
AccessioniPrimary (citable) accession number: Q9ZVQ3
Secondary accession number(s): Q0WWK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.