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Protein

General transcription factor IIH subunit 2

Gene

GTF2H2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the general transcription and DNA repair factor IIH (TFIIH) core, which is essential for transcription, and is involved in nucleotide excision repair (NER) of damaged DNA. Can restore UV resistance in the NER-deficient ssl1-1 yeast mutant.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri362 – 40847RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • nucleotide-excision repair Source: TAIR
  • regulation of transcription from RNA polymerase II promoter Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair, Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-113418. Formation of the Early Elongation Complex.
R-ATH-5696395. Formation of Incision Complex in GG-NER.
R-ATH-5696400. Dual Incision in GG-NER.
R-ATH-674695. RNA Polymerase II Pre-transcription Events.
R-ATH-6781823. Formation of TC-NER Pre-Incision Complex.
R-ATH-6782135. Dual incision in TC-NER.
R-ATH-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-ATH-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-ATH-72086. mRNA Capping.
R-ATH-73776. RNA Polymerase II Promoter Escape.
R-ATH-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-ATH-75953. RNA Polymerase II Transcription Initiation.
R-ATH-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-ATH-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription factor IIH subunit 2Curated
Short name:
AtGTF2H21 Publication
Alternative name(s):
TFIIH basal transcription factor complex p44 subunitCurated
Short name:
Atp441 Publication
Gene namesi
Name:GTF2H21 Publication
Synonyms:P44Curated
Ordered Locus Names:At1g05055Imported
ORF Names:T7A14.8Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G05055.

Subcellular locationi

GO - Cellular componenti

  • core TFIIH complex Source: InterPro
  • holo TFIIH complex Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 421421General transcription factor IIH subunit 2PRO_0000435434Add
BLAST

Proteomic databases

PaxDbiQ9ZVN9.

Interactioni

Subunit structurei

Component of the TFIIH core complex, which is composed of 7 subunits, XPD, XPB, TFB1/GTF2H1, GTF2H2/P44, TFB4/GTF2H3, TFB2/GTF2H4 and TFB5/GTF2H5 (Probable). Interacts with XPD (PubMed:16623910).1 Publication1 Publication

Protein-protein interaction databases

STRINGi3702.AT1G05055.1.

Structurei

3D structure databases

ProteinModelPortaliQ9ZVN9.
SMRiQ9ZVN9. Positions 77-261, 342-407.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini83 – 272190VWFAPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the GTF2H2 family.Curated
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
Contains 1 VWFA domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri362 – 40847RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2807. Eukaryota.
COG5151. LUCA.
HOGENOMiHOG000159415.
KOiK03142.
OMAiIRMGFPH.
PhylomeDBiQ9ZVN9.

Family and domain databases

InterProiIPR007198. Ssl1-like.
IPR004595. TFIIH_C1-like_dom.
IPR012170. TFIIH_SSL1/p44.
IPR002035. VWF_A.
IPR001841. Znf_RING.
[Graphical view]
PANTHERiPTHR12695:SF2. PTHR12695:SF2. 1 hit.
PfamiPF07975. C1_4. 1 hit.
PF04056. Ssl1. 1 hit.
[Graphical view]
PIRSFiPIRSF015919. TFIIH_SSL1. 1 hit.
SMARTiSM01047. C1_4. 1 hit.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
TIGRFAMsiTIGR00622. ssl1. 1 hit.
PROSITEiPS50234. VWFA. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZVN9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNQRKRSND EREEEDDEDA EGIGEWERAY VDDRSWEELQ EDESGLLRPI
60 70 80 90 100
DNSAIYHAQY RRRLRMLSAA AAGTRIQKGL IRYLYIVIDF SRAAAEMDFR
110 120 130 140 150
PSRMAIMAKH VEAFIREFFD QNPLSQIGLV SIKNGVAHTL TDLGGSPETH
160 170 180 190 200
IKALMGKLEA LGDSSLQNAL ELVHEHLNQV PSYGHREVLI LYSALCTCDP
210 220 230 240 250
GDIMETIQKC KKSKLRCSVI GLSAEMFICK HLCQETGGLY SVAVDEVHLK
260 270 280 290 300
DLLLEHAPPP PAIAEFAIAN LIKMGFPQRA AEGSMAICSC HKEVKIGAGY
310 320 330 340 350
MCPRCKARVC DLPTECTICG LTLVSSPHLA RSYHHLFPIA PFDEVPALSS
360 370 380 390 400
LNDNRRKLGK SCFGCQQSLI GAGNKPVPCV TCRKCKHYFC LDCDIYIHES
410 420
LHNCPGCESI HRPKSVSLME E
Length:421
Mass (Da):46,978
Last modified:May 1, 1999 - v1
Checksum:i20A863C08776F4E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF499443 mRNA. Translation: AAM90909.1.
AC005322 Genomic DNA. Translation: AAC97995.1.
CP002684 Genomic DNA. Translation: AEE27784.1.
AK229479 mRNA. Translation: BAF01337.1.
PIRiE86184.
RefSeqiNP_683275.2. NM_148434.3.
UniGeneiAt.43339.

Genome annotation databases

EnsemblPlantsiAT1G05055.1; AT1G05055.1; AT1G05055.
GeneIDi839332.
GrameneiAT1G05055.1; AT1G05055.1; AT1G05055.
KEGGiath:AT1G05055.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF499443 mRNA. Translation: AAM90909.1.
AC005322 Genomic DNA. Translation: AAC97995.1.
CP002684 Genomic DNA. Translation: AEE27784.1.
AK229479 mRNA. Translation: BAF01337.1.
PIRiE86184.
RefSeqiNP_683275.2. NM_148434.3.
UniGeneiAt.43339.

3D structure databases

ProteinModelPortaliQ9ZVN9.
SMRiQ9ZVN9. Positions 77-261, 342-407.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G05055.1.

Proteomic databases

PaxDbiQ9ZVN9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G05055.1; AT1G05055.1; AT1G05055.
GeneIDi839332.
GrameneiAT1G05055.1; AT1G05055.1; AT1G05055.
KEGGiath:AT1G05055.

Organism-specific databases

TAIRiAT1G05055.

Phylogenomic databases

eggNOGiKOG2807. Eukaryota.
COG5151. LUCA.
HOGENOMiHOG000159415.
KOiK03142.
OMAiIRMGFPH.
PhylomeDBiQ9ZVN9.

Enzyme and pathway databases

ReactomeiR-ATH-113418. Formation of the Early Elongation Complex.
R-ATH-5696395. Formation of Incision Complex in GG-NER.
R-ATH-5696400. Dual Incision in GG-NER.
R-ATH-674695. RNA Polymerase II Pre-transcription Events.
R-ATH-6781823. Formation of TC-NER Pre-Incision Complex.
R-ATH-6782135. Dual incision in TC-NER.
R-ATH-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-ATH-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-ATH-72086. mRNA Capping.
R-ATH-73776. RNA Polymerase II Promoter Escape.
R-ATH-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-ATH-75953. RNA Polymerase II Transcription Initiation.
R-ATH-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-ATH-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Miscellaneous databases

PROiQ9ZVN9.

Family and domain databases

InterProiIPR007198. Ssl1-like.
IPR004595. TFIIH_C1-like_dom.
IPR012170. TFIIH_SSL1/p44.
IPR002035. VWF_A.
IPR001841. Znf_RING.
[Graphical view]
PANTHERiPTHR12695:SF2. PTHR12695:SF2. 1 hit.
PfamiPF07975. C1_4. 1 hit.
PF04056. Ssl1. 1 hit.
[Graphical view]
PIRSFiPIRSF015919. TFIIH_SSL1. 1 hit.
SMARTiSM01047. C1_4. 1 hit.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
TIGRFAMsiTIGR00622. ssl1. 1 hit.
PROSITEiPS50234. VWFA. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis homologue of human transcription factor IIH/nucleotide excision repair factor p44 can function in transcription and DNA repair and interacts with AtXPD."
    Vonarx E.J., Tabone E.K., Osmond M.J., Anderson H.J., Kunz B.A.
    Plant J. 46:512-521(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH XPD.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Components of nucleotide excision repair and DNA damage tolerance in Arabidopsis thaliana."
    Kunz B.A., Anderson H.J., Osmond M.J., Vonarx E.J.
    Environ. Mol. Mutagen. 45:115-127(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPONENT OF TFIIH CORE COMPLEX, NOMENCLATURE.

Entry informationi

Entry nameiTF2H2_ARATH
AccessioniPrimary (citable) accession number: Q9ZVN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 17, 2016
Last sequence update: May 1, 1999
Last modified: June 8, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.