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Protein

General transcription factor IIH subunit 2

Gene

GTF2H2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription (By similarity). Can restore UV resistance in the NER-deficient ssl1-1 yeast mutant (PubMed:16623910).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri362 – 408RING-typePROSITE-ProRule annotationAdd BLAST47

GO - Molecular functioni

GO - Biological processi

  • nucleotide-excision repair Source: TAIR
  • regulation of transcription by RNA polymerase II Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Biological processDNA damage, DNA repair, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-113418 Formation of the Early Elongation Complex
R-ATH-5696395 Formation of Incision Complex in GG-NER
R-ATH-5696400 Dual Incision in GG-NER
R-ATH-674695 RNA Polymerase II Pre-transcription Events
R-ATH-6781823 Formation of TC-NER Pre-Incision Complex
R-ATH-6782135 Dual incision in TC-NER
R-ATH-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-ATH-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-ATH-72086 mRNA Capping
R-ATH-73776 RNA Polymerase II Promoter Escape
R-ATH-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-ATH-75953 RNA Polymerase II Transcription Initiation
R-ATH-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-ATH-77075 RNA Pol II CTD phosphorylation and interaction with CE

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription factor IIH subunit 2Curated
Short name:
AtGTF2H21 Publication
Alternative name(s):
TFIIH basal transcription factor complex p44 subunitCurated
Short name:
Atp441 Publication
Gene namesi
Name:GTF2H21 Publication
Synonyms:P44Curated
Ordered Locus Names:At1g05055Imported
ORF Names:T7A14.8Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G05055
TAIRilocus:504956103 AT1G05055

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004354341 – 421General transcription factor IIH subunit 2Add BLAST421

Proteomic databases

PaxDbiQ9ZVN9

Expressioni

Gene expression databases

ExpressionAtlasiQ9ZVN9 baseline and differential

Interactioni

Subunit structurei

Component of the 7-subunit TFIIH core complex composed of XPB, XPD, TFB1/GTF2H1, GTF2H2/P44, TFB4/GTF2H3, TFB2/GTF2H4 and TFB5/GTF2H5, which is active in NER. The core complex associates with the 3-subunit CDK-activating kinase (CAK) module composed of CYCH1/cyclin H1, CDKD and MAT1/At4g30820 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription (By similarity). Interacts with XPD (PubMed:16623910).By similarity1 Publication1 Publication

Protein-protein interaction databases

STRINGi3702.AT1G05055.1

Structurei

3D structure databases

ProteinModelPortaliQ9ZVN9
SMRiQ9ZVN9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini83 – 272VWFAPROSITE-ProRule annotationAdd BLAST190

Sequence similaritiesi

Belongs to the GTF2H2 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri362 – 408RING-typePROSITE-ProRule annotationAdd BLAST47

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2807 Eukaryota
COG5151 LUCA
HOGENOMiHOG000159415
KOiK03142
OMAiDCDIFIH
OrthoDBiEOG09360CGS
PhylomeDBiQ9ZVN9

Family and domain databases

CDDicd01453 vWA_transcription_factor_IIH_t, 1 hit
Gene3Di3.30.40.10, 1 hit
3.40.50.410, 1 hit
InterProiView protein in InterPro
IPR007198 Ssl1-like
IPR004595 TFIIH_C1-like_dom
IPR012170 TFIIH_SSL1/p44
IPR002035 VWF_A
IPR036465 vWFA_dom_sf
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR12695 PTHR12695, 1 hit
PfamiView protein in Pfam
PF07975 C1_4, 1 hit
PF04056 Ssl1, 1 hit
PIRSFiPIRSF015919 TFIIH_SSL1, 1 hit
SMARTiView protein in SMART
SM01047 C1_4, 1 hit
SM00327 VWA, 1 hit
SUPFAMiSSF53300 SSF53300, 1 hit
TIGRFAMsiTIGR00622 ssl1, 1 hit
PROSITEiView protein in PROSITE
PS50234 VWFA, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9ZVN9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNQRKRSND EREEEDDEDA EGIGEWERAY VDDRSWEELQ EDESGLLRPI
60 70 80 90 100
DNSAIYHAQY RRRLRMLSAA AAGTRIQKGL IRYLYIVIDF SRAAAEMDFR
110 120 130 140 150
PSRMAIMAKH VEAFIREFFD QNPLSQIGLV SIKNGVAHTL TDLGGSPETH
160 170 180 190 200
IKALMGKLEA LGDSSLQNAL ELVHEHLNQV PSYGHREVLI LYSALCTCDP
210 220 230 240 250
GDIMETIQKC KKSKLRCSVI GLSAEMFICK HLCQETGGLY SVAVDEVHLK
260 270 280 290 300
DLLLEHAPPP PAIAEFAIAN LIKMGFPQRA AEGSMAICSC HKEVKIGAGY
310 320 330 340 350
MCPRCKARVC DLPTECTICG LTLVSSPHLA RSYHHLFPIA PFDEVPALSS
360 370 380 390 400
LNDNRRKLGK SCFGCQQSLI GAGNKPVPCV TCRKCKHYFC LDCDIYIHES
410 420
LHNCPGCESI HRPKSVSLME E
Length:421
Mass (Da):46,978
Last modified:May 1, 1999 - v1
Checksum:i20A863C08776F4E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF499443 mRNA Translation: AAM90909.1
AC005322 Genomic DNA Translation: AAC97995.1
CP002684 Genomic DNA Translation: AEE27784.1
AK229479 mRNA Translation: BAF01337.1
PIRiE86184
RefSeqiNP_683275.2, NM_148434.4
UniGeneiAt.43339

Genome annotation databases

EnsemblPlantsiAT1G05055.1; AT1G05055.1; AT1G05055
GeneIDi839332
GrameneiAT1G05055.1; AT1G05055.1; AT1G05055
KEGGiath:AT1G05055

Similar proteinsi

Entry informationi

Entry nameiTF2H2_ARATH
AccessioniPrimary (citable) accession number: Q9ZVN9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 17, 2016
Last sequence update: May 1, 1999
Last modified: May 23, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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