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Protein

Tyrosine-protein phosphatase DSP1

Gene

DSP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Possesses phosphotyrosine phosphatase activity in vitro. Hydrolyzes para-nitrophenyl phosphate in vitro (PubMed:21409566, PubMed:18433060). Hydrolyzes O-methylfluorescein phosphate in vitro (PubMed:21409566). Hydrolyzes polyphosphate and ATP in vitro (PubMed:18433060). Dephosphorylates the phosphoinositides PI(3,4,5)P3, PI(3,5)P2, but not PI3P, PI(3,4)P2 or PI(4,5)P2 (PubMed:17976645).3 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation2 Publications

Kineticsi

  1. KM=0.063 mM for polyphoshate1 Publication
  2. KM=0.38 mM for ATP1 Publication
  3. KM=0.88 mM for phosphotyrosine1 Publication
  4. KM=0.67 mM for para-nitrophenyl phosphate1 Publication

    pH dependencei

    Optimum pH is 5.0 (with polyphosphate as substrate).1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei150Phosphocysteine intermediatePROSITE-ProRule annotation1

    GO - Molecular functioni

    • phosphatase activity Source: UniProtKB
    • protein tyrosine phosphatase activity Source: GO_Central

    Keywordsi

    Molecular functionHydrolase, Protein phosphatase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosine-protein phosphatase DSP1Curated (EC:3.1.3.48PROSITE-ProRule annotation2 Publications)
    Alternative name(s):
    Protein PLANT AND FUNGI ATYPICAL DUAL-SPECIFICITY PHOSPHATASE 11 Publication
    Short name:
    AtPFA-DSP11 Publication
    Tyrosine-protein phosphatase At1g05000Curated
    Gene namesi
    Name:DSP1Curated
    Synonyms:PTP135Imported
    Ordered Locus Names:At1g05000
    ORF Names:T7A14.14
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 1

    Organism-specific databases

    AraportiAT1G05000

    Subcellular locationi

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi117E → A: Reduces phosphatase activity toward para-nitrophenyl phosphate 2-fold. 1 Publication1
    Mutagenesisi150C → S: Abolishes tyrosine-protein phosphatase activity. 1 Publication1
    Mutagenesisi155H → G or S: Abolishes phosphatase activity toward the phosphoinositides PI(3,4,5)P3 and PI(3,5)P2; reduces phosphatase activity toward para-nitrophenyl phosphate and O-methylfluorescein phosphate. 1 Publication1
    Mutagenesisi191D → A: Reduces phosphatase activity toward para-nitrophenyl phosphate 2-fold. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000949231 – 215Tyrosine-protein phosphatase DSP1Add BLAST215

    Expressioni

    Tissue specificityi

    Highly expressed in siliques and at lower levels in roots, leaves and flowers.1 Publication

    Gene expression databases

    ExpressionAtlasiQ9ZVN4 baseline and differential
    GenevisibleiQ9ZVN4 AT

    Interactioni

    Subunit structurei

    Homomultimer (Probable). Homodimer and homohexamer (PubMed:18433060).Curated1 Publication

    Protein-protein interaction databases

    BioGridi24583, 17 interactors

    Structurei

    Secondary structure

    1215
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi60 – 63Combined sources4
    Beta strandi66 – 70Combined sources5
    Helixi74 – 83Combined sources10
    Beta strandi86 – 90Combined sources5
    Helixi98 – 107Combined sources10
    Beta strandi110 – 113Combined sources4
    Helixi121 – 123Combined sources3
    Helixi128 – 139Combined sources12
    Helixi141 – 143Combined sources3
    Beta strandi145 – 149Combined sources5
    Beta strandi151 – 155Combined sources5
    Helixi156 – 167Combined sources12
    Helixi172 – 183Combined sources12
    Helixi184 – 186Combined sources3
    Helixi189 – 196Combined sources8

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1XRIX-ray3.30A/B52-202[»]
    2Q47X-ray3.30A/B52-202[»]
    ProteinModelPortaliQ9ZVN4
    SMRiQ9ZVN4
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9ZVN4

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi7 – 10Poly-Thr4

    Sequence similaritiesi

    Phylogenomic databases

    HOGENOMiHOG000188365
    InParanoidiQ9ZVN4
    KOiK18045
    PhylomeDBiQ9ZVN4

    Family and domain databases

    Gene3Di3.90.190.10, 1 hit
    InterProiView protein in InterPro
    IPR020428 PFA-DSPs
    IPR029021 Prot-tyrosine_phosphatase-like
    IPR004861 Siw14-like
    IPR016130 Tyr_Pase_AS
    PfamiView protein in Pfam
    PF03162 Y_phosphatase2, 1 hit
    PRINTSiPR01911 PFDSPHPHTASE
    SUPFAMiSSF52799 SSF52799, 1 hit
    PROSITEiView protein in PROSITE
    PS00383 TYR_PHOSPHATASE_1, 1 hit

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

    Note: A number of isoforms are produced. According to EST sequences.
    Isoform 1 (identifier: Q9ZVN4-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MKLVEKTTTT EQDNGEDFCR TIIEVSEVNR NVFQAPGGEA DPFRVVSGEE
    60 70 80 90 100
    LHLIPPLNFS MVDNGIFRSG FPDSANFSFL QTLGLRSIIY LCPEPYPESN
    110 120 130 140 150
    LQFLKSNGIR LFQFGIEGNK EPFVNIPDHK IRMALKVLLD EKNHPVLIHC
    160 170 180 190 200
    KRGKHRTGCL VGCLRKLQKW CLTSIFDEYQ RFAAAKARVS DQRFMEIFDV
    210
    SSFSHIPMSF SCSIR
    Length:215
    Mass (Da):24,537
    Last modified:May 1, 1999 - v1
    Checksum:i24BCB3033CBCFA3A
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    FJ605097 Genomic DNA Translation: ACU43461.1
    AC005322 Genomic DNA Translation: AAC97999.1
    CP002684 Genomic DNA Translation: AEE27777.1
    AK228069 mRNA Translation: BAF00029.1
    BT005210 mRNA Translation: AAO63274.1
    PIRiG86183
    RefSeqiNP_171993.1, NM_100379.3 [Q9ZVN4-1]
    UniGeneiAt.43231

    Genome annotation databases

    EnsemblPlantsiAT1G05000.1; AT1G05000.1; AT1G05000 [Q9ZVN4-1]
    GeneIDi839348
    GrameneiAT1G05000.1; AT1G05000.1; AT1G05000 [Q9ZVN4-1]
    KEGGiath:AT1G05000

    Keywords - Coding sequence diversityi

    Alternative splicing

    Similar proteinsi

    Entry informationi

    Entry nameiDSP1_ARATH
    AccessioniPrimary (citable) accession number: Q9ZVN4
    Secondary accession number(s): Q0WS70
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
    Last sequence update: May 1, 1999
    Last modified: April 25, 2018
    This is version 107 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

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