Q9ZVM9 (Y1461_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable serine/threonine-protein kinase At1g54610
EC=2.7.11.1

Gene names

Ordered Locus Names:	At1g54610
ORF Names:	T22H22.5

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Post-translational modification	Phosphorylation level varies significantly during early response to general elicitors.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	plasma membrane Inferred from direct assay Ref.4. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CAM1	P25854	2	EBI-1235713,EBI-1235664
CAM7	P59220	2	EBI-1235713,EBI-1236031

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9ZVM9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 572	572	Probable serine/threonine-protein kinase At1g54610		PRO_0000305183
Regions
	Domain	118 – 402	285	Protein kinase
	Nucleotide binding	124 – 132	9	ATP By similarity
	Compositional bias	11 – 14	4	Poly-Thr
	Compositional bias	29 – 32	4	Poly-Val
Sites
	Active site	242	1	Proton acceptor By similarity
	Binding site	147	1	ATP By similarity
Amino acid modifications
	Modified residue	36	1	Phosphoserine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 3C7072B7184310C9	FASTA	572	63,288
	10 20 30 40 50 60 MGCVFGREAA TTTTAEAKQA KSSKASSGVV VVGESSVTKS NGVIADDVEK KKNEEANGDK 70 80 90 100 110 120 ERKSSKGDRR RSTKPNPRLS NPSKHWRGEQ VAAGWPSWLS DACGEALNGW VPRKADTFEK 130 140 150 160 170 180 IDKIGQGTYS NVYKAKDMLT GKIVALKKVR FDNLEPESVK FMAREILVLR RLDHPNVVKL 190 200 210 220 230 240 EGLVTSRMSC SLYLVFQYMD HDLAGLASSP VVKFSESEVK CLMRQLISGL EHCHSRGVLH 250 260 270 280 290 300 RDIKGSNLLI DDGGVLKIAD FGLATIFDPN HKRPMTSRVV TLWYRAPELL LGATDYGVGI 310 320 330 340 350 360 DLWSAGCILA ELLAGRPIMP GRTEVEQLHK IYKLCGSPSE DYWKKGKFTH GAIYKPREPY 370 380 390 400 410 420 KRSIRETFKD FPPSSLPLID ALLSIEPEDR QTASAALKSE FFTSEPYACE PADLPKYPPS 430 440 450 460 470 480 KEIDAKRRDE ETRRQRAASK AQGDGARKNR HRDRSNRALP APEANAELQS NVDRRRLITH 490 500 510 520 530 540 ANAKSKSEKF PPPHQDGGAM GVPLGASQHI DPTFIPRDMV PSFTSSSFNF SKDEPPTQVQ 550 560 570 TWSGPLGHPI TGVSRKKKDN TKSSKGKRAV VA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Quantitative phosphoproteomics of early elicitor signaling in Arabidopsis." Benschop J.J., Mohammed S., O'Flaherty M., Heck A.J.R., Slijper M., Menke F.L.H. Mol. Cell. Proteomics 6:1198-1214(2007) [PubMed: 17317660] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, MASS SPECTROMETRY. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC005388 Genomic DNA. Translation: AAC64876.1. CP002684 Genomic DNA. Translation: AEE33123.1. CP002684 Genomic DNA. Translation: AEE33125.1. AF370510 mRNA. Translation: AAK43887.1. AY128918 mRNA. Translation: AAM91318.1.
IPI	IPI00534133.
PIR	B96588.
RefSeq	NP_001117490.1. NM_001124018.1. NP_175862.1. NM_104338.6.
UniGene	At.21069.
3D structure databases
HSSP	HSSP built from PDB template 2CJM based on UniProtKB P24941.
ProteinModelPortal	Q9ZVM9.
SMR	Q9ZVM9. Positions 114-409.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9ZVM9. 8 interactions.
Proteomic databases
PRIDE	Q9ZVM9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G54610.1; AT1G54610.1; AT1G54610. AT1G54610.3; AT1G54610.3; AT1G54610.
GeneID	841903.
GenomeReviews	Gene locus AT1G54610 in contig CT485782_GR.
KEGG	ath:AT1G54610.
NMPDR	fig|3702.1.peg.4981.
Organism-specific databases
TAIR	At1g54610.
Phylogenomic databases
GeneTree	EPGT00050000000065.
HOGENOM	HBG755340.
InParanoid	Q9ZVM9.
OMA	DAIGDWT.
PhylomeDB	Q9ZVM9.
ProtClustDB	CLSN2682421.
Gene expression databases
ArrayExpress	Q9ZVM9.
Genevestigator	Q9ZVM9.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Y1461_ARATH

Accession

Primary (citable) accession number: Q9ZVM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	May 1, 1999
Last modified:	December 14, 2011
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program