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Protein

Protein argonaute 4

Gene

AGO4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Together with RDM3, required for transcriptional gene silencing (TGS) by DNA methylation and repressive histone modifications (H3K9me2) of several chromatin loci (PubMed:21738482). Component of the RISC complex that associate with the small interfering RNA (siRNA) pathway involved in direct cytosine methylation at endogenous DNA repeats. Forms a AGO4/NRPE1/siRNA complex in cajal body, facilitating its function in RNA-directed gene silencing of target loci. Required for CpNpG and asymmetric DNA methylation as well as histone H3 'Lys-9' methylation (H3K9me) at SUP and SN1 loci. May be not required for CpG methylation. Required for the production and maintenance of retrotransposon SN1 and Copia and ribosomal 5S 25 nucleotide siRNAs specialized in gene silencing at chromatin level. Involved in de novo methylation of FWA gene and required for the maintenance of RNA-directed DNA methylation (RdDM) triggered by inverted repeat transgenes. Interacts with miRNA miR390 and miR172, targeting respectively TAS3 and AP2 mRNAs, and mediates cleavage of miRNA targets. Associates mainly with small RNAs of 24 nucleotide in length and preferentially recruits small RNAs with a 5' terminal adenosine. Targeted by the turnip yellows virus (TuYV) protein P0 (via F-box-like domain) for probable proteasome degradation and thereby inactivating AGO4 function in RNA silencing. Required for resistance to the bacterial pathogen P.syringae. Works independently of the RdDM pathway in mediating resistance to P.syringae. RdDM is involved in viral genome methylation as an epigenetic defense against geminiviruses (PubMed:12522258, PubMed:14988555, PubMed:15242620, PubMed:16839878, PubMed:16839879, PubMed:16998468, PubMed:17869110, PubMed:17938239, PubMed:17993621, PubMed:18342361, PubMed:18596098, PubMed:19377477, PubMed:20173091).14 Publications

GO - Molecular functioni

  • chromatin binding Source: UniProtKB
  • endoribonuclease activity Source: CAFA
  • miRNA binding Source: CAFA
  • siRNA binding Source: TAIR

GO - Biological processi

  • chromatin silencing Source: UniProtKB
  • chromatin silencing by small RNA Source: TAIR
  • defense response to bacterium, incompatible interaction Source: TAIR
  • defense response to virus Source: TAIR
  • DNA methylation Source: TAIR
  • gene silencing Source: UniProtKB
  • histone H3-K9 methylation Source: TAIR
  • modulation by virus of host morphology or physiology Source: TAIR
  • production of siRNA involved in chromatin silencing by small RNA Source: CAFA
  • production of siRNA involved in RNA interference Source: TAIR
  • regulation of translation Source: UniProtKB-KW
  • RNA-directed DNA methylation Source: CAFA
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionRepressor, Ribonucleoprotein, RNA-binding
Biological processHost-virus interaction, Plant defense, RNA-mediated gene silencing, Transcription, Transcription regulation, Translation regulation

Protein family/group databases

MoonProtiQ9ZVD5.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein argonaute 4
Alternative name(s):
Protein OVEREXPRESSOR OF CATIONIC PEROXIDASE 11
Gene namesi
Name:AGO4
Synonyms:OCP11
Ordered Locus Names:At2g27040
ORF Names:T20P8.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G27040.
TAIRilocus:2059258. AT2G27040.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Decreased DNA cytosine methylation at CpNpG and asymmetric positions at different DNA loci corresponding to retroelements, transposons and repetitive DNA sequences (PubMed:12522258, PubMed:17993621, PubMed:21738482). Reduced H3K9me2 at IGN5 and IGN26 loci (PubMed:21738482).3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi641E → K in ago4-2; no effect on RNA binding, but loss of slicer activity. 1 Publication1
Mutagenesisi660D → A: No effect on RNA binding, but loss of slicer activity. 1 Publication1
Mutagenesisi742D → A: No effect on RNA binding, but loss of slicer activity. 1 Publication1
Mutagenesisi874H → A: No effect on RNA binding, but loss of slicer activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004046671 – 924Protein argonaute 4Add BLAST924

Proteomic databases

PaxDbiQ9ZVD5.
PRIDEiQ9ZVD5.

PTM databases

iPTMnetiQ9ZVD5.

Expressioni

Tissue specificityi

Expressed in embryos, mature leaves, vascular tissue of the sepals, stamens and stigma, at the tip of the style and siliques.1 Publication

Gene expression databases

GenevisibleiQ9ZVD5. AT.

Interactioni

Subunit structurei

Interacts with NRPE1 (via C-terminus). Binding to NRPE1 is required for its function in RdDM. Interacts with turnip crinkle virus (TCV) capsid protein P38; this interaction inhibits probably RNA silencing ability of AGO4. Interacts with SDE3 (PubMed:16839879, PubMed:17938239, PubMed:19377477, PubMed:20439431, PubMed:22940249). Binds to RDM3 (PubMed:19410546, PubMed:19343051). Binds chromatin at loci subject to transcriptional silencing (PubMed:21738482).8 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi2598. 4 interactors.
DIPiDIP-53402N.
IntActiQ9ZVD5. 6 interactors.
MINTiMINT-7222821.
STRINGi3702.AT2G27040.1.

Structurei

3D structure databases

ProteinModelPortaliQ9ZVD5.
SMRiQ9ZVD5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini272 – 408PAZPROSITE-ProRule annotationAdd BLAST137
Domaini577 – 885PiwiPROSITE-ProRule annotationAdd BLAST309

Sequence similaritiesi

Belongs to the argonaute family. Ago subfamily.Curated

Phylogenomic databases

eggNOGiKOG1041. Eukaryota.
ENOG410XP07. LUCA.
HOGENOMiHOG000116043.
InParanoidiQ9ZVD5.
KOiK11593.
OMAiCYEQMFT.
OrthoDBiEOG093601I2.
PhylomeDBiQ9ZVD5.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiView protein in InterPro
IPR014811. ArgoL1.
IPR032472. ArgoL2.
IPR032473. Argonaute_Mid_dom.
IPR032474. Argonaute_N.
IPR003100. PAZ_dom.
IPR036085. PAZ_dom_sf.
IPR003165. Piwi.
IPR012337. RNaseH-like_sf.
IPR036397. RNaseH_sf.
PfamiView protein in Pfam
PF08699. ArgoL1. 1 hit.
PF16488. ArgoL2. 1 hit.
PF16487. ArgoMid. 1 hit.
PF16486. ArgoN. 1 hit.
PF02170. PAZ. 1 hit.
PF02171. Piwi. 1 hit.
SMARTiView protein in SMART
SM01163. DUF1785. 1 hit.
SM00950. Piwi. 1 hit.
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEiView protein in PROSITE
PS50821. PAZ. 1 hit.
PS50822. PIWI. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9ZVD5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSTNGNGAD LESANGANGS GVTEALPPPP PVIPPNVEPV RVKTELAEKK
60 70 80 90 100
GPVRVPMARK GFGTRGQKIP LLTNHFKVDV ANLQGHFFHY SVALFYDDGR
110 120 130 140 150
PVEQKGVGRK ILDKVHQTYH SDLDGKEFAY DGEKTLFTYG ALPSNKMDFS
160 170 180 190 200
VVLEEVSATR ANGNGSPNGN ESPSDGDRKR LRRPNRSKNF RVEISYAAKI
210 220 230 240 250
PLQALANAMR GQESENSQEA IRVLDIILRQ HAARQGCLLV RQSFFHNDPT
260 270 280 290 300
NCEPVGGNIL GCRGFHSSFR TTQGGMSLNM DVTTTMIIKP GPVVDFLIAN
310 320 330 340 350
QNARDPYSID WSKAKRTLKN LRVKVSPSGQ EFKITGLSDK PCREQTFELK
360 370 380 390 400
KRNPNENGEF ETTEVTVADY FRDTRHIDLQ YSADLPCINV GKPKRPTYIP
410 420 430 440 450
LELCALVPLQ RYTKALTTFQ RSALVEKSRQ KPQERMTVLS KALKVSNYDA
460 470 480 490 500
EPLLRSCGIS ISSNFTQVEG RVLPAPKLKM GCGSETFPRN GRWNFNNKEF
510 520 530 540 550
VEPTKIQRWV VVNFSARCNV RQVVDDLIKI GGSKGIEIAS PFQVFEEGNQ
560 570 580 590 600
FRRAPPMIRV ENMFKDIQSK LPGVPQFILC VLPDKKNSDL YGPWKKKNLT
610 620 630 640 650
EFGIVTQCMA PTRQPNDQYL TNLLLKINAK LGGLNSMLSV ERTPAFTVIS
660 670 680 690 700
KVPTIILGMD VSHGSPGQSD VPSIAAVVSS REWPLISKYR ASVRTQPSKA
710 720 730 740 750
EMIESLVKKN GTEDDGIIKE LLVDFYTSSN KRKPEHIIIF RDGVSESQFN
760 770 780 790 800
QVLNIELDQI IEACKLLDAN WNPKFLLLVA QKNHHTKFFQ PTSPENVPPG
810 820 830 840 850
TIIDNKICHP KNNDFYLCAH AGMIGTTRPT HYHVLYDEIG FSADELQELV
860 870 880 890 900
HSLSYVYQRS TSAISVVAPI CYAHLAAAQL GTFMKFEDQS ETSSSHGGIT
910 920
APGPISVAQL PRLKDNVANS MFFC
Length:924
Mass (Da):102,840
Last modified:June 1, 2002 - v2
Checksum:iC0323FAE9592B147
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005623 Genomic DNA. Translation: AAC77862.2.
CP002685 Genomic DNA. Translation: AEC07928.1.
CP002685 Genomic DNA. Translation: AEC07929.1.
AY035081 mRNA. Translation: AAK59586.1.
AY051033 mRNA. Translation: AAK93710.1.
PIRiA84668.
RefSeqiNP_001189613.1. NM_001202684.1.
NP_565633.1. NM_128262.4.
UniGeneiAt.13165.

Genome annotation databases

EnsemblPlantsiAT2G27040.1; AT2G27040.1; AT2G27040.
AT2G27040.2; AT2G27040.2; AT2G27040.
GeneIDi817246.
GrameneiAT2G27040.1; AT2G27040.1; AT2G27040.
AT2G27040.2; AT2G27040.2; AT2G27040.
KEGGiath:AT2G27040.

Similar proteinsi

Entry informationi

Entry nameiAGO4_ARATH
AccessioniPrimary (citable) accession number: Q9ZVD5
Secondary accession number(s): Q93VG2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: June 1, 2002
Last modified: October 25, 2017
This is version 114 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families