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Protein

Serrate RNA effector molecule

Gene

SE

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a mediator between the cap-binding complex (CBC) and both the pre-mRNA splicing and primary microRNAs (miRNAs) processing machinery. Required for proper processing of primary miRNAs to miRNAs, thereby playing a role in RNA-mediated gene silencing (RNAi) by miRNAs. Does not participate in sense post-transcriptional gene silencing. Acts as a regulator of meristem activity and adaxial leaf fate via the miRNA gene-silencing pathway by regulating the expression of PHB and by limiting the competence of shoot tissue to respond to KNOX expression. Its function is however not limited to miRNA-mediated repression of leaf polarity genes, but rather acts as a general regulator of primary microRNAs processing. Also critical for the accumulation of the trans-acting small interfering RNA (ta-siRNA). Required for pre-mRNA splicing.5 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri498 – 523C2H2-typeAdd BLAST26

GO - Molecular functioni

  • DNA binding Source: TAIR
  • DNA binding transcription factor activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • mRNA processing Source: UniProtKB-KW
  • primary miRNA processing Source: UniProtKB
  • production of ta-siRNAs involved in RNA interference Source: TAIR
  • regulation of adaxial/abaxial pattern formation Source: TAIR
  • regulation of alternative mRNA splicing, via spliceosome Source: TAIR
  • regulation of meristem development Source: TAIR
  • regulation of transcription, DNA-templated Source: TAIR
  • RNA splicing Source: UniProtKB
  • shoot system development Source: TAIR

Keywordsi

Biological processmRNA processing, mRNA splicing, RNA-mediated gene silencing
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Serrate RNA effector molecule
Gene namesi
Name:SE
Ordered Locus Names:At2g27100
ORF Names:T20P8.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G27100.
TAIRilocus:2059294. AT2G27100.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Death during embryogenesis. Weaker mutants (se-1, se-2 and se-3) also exist. Mutant se-1 displays defects in shoot and leaf development. Mutants se-2 and se-3 show adaxial leaf curling, loss of asymmetric differentiation of abaxial and adaxial cell types and development of trumpet-shaped or radial leaves. Vascular polarity of se-3 leaves is also perturbed, with xylem elements forming both adaxially and abaxially in the vascular bundle.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003852281 – 720Serrate RNA effector moleculeAdd BLAST720

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei76PhosphoserineCombined sources1
Modified residuei90PhosphoserineCombined sources1
Modified residuei92PhosphoserineCombined sources1
Modified residuei689PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9ZVD0.
PRIDEiQ9ZVD0.

PTM databases

iPTMnetiQ9ZVD0.

Expressioni

Tissue specificityi

Expressed in shoot meristems and in emerging organ primordia throughout development.1 Publication

Gene expression databases

ExpressionAtlasiQ9ZVD0. baseline and differential.
GenevisibleiQ9ZVD0. AT.

Interactioni

Subunit structurei

Interacts with HYL1 (PubMed:16889646, PubMed:16977334). Interacts with RCF3, RS40 and RS41 (PubMed:26227967).3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi2604. 12 interactors.
DIPiDIP-58979N.
IntActiQ9ZVD0. 2 interactors.
STRINGi3702.AT2G27100.1.

Structurei

Secondary structure

1720
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi198 – 201Combined sources4
Helixi202 – 204Combined sources3
Turni205 – 207Combined sources3
Helixi211 – 237Combined sources27
Helixi241 – 247Combined sources7
Helixi249 – 275Combined sources27
Beta strandi283 – 286Combined sources4
Helixi331 – 333Combined sources3
Helixi336 – 357Combined sources22
Beta strandi386 – 388Combined sources3
Beta strandi395 – 397Combined sources3
Helixi400 – 414Combined sources15
Helixi418 – 420Combined sources3
Helixi446 – 460Combined sources15
Helixi464 – 469Combined sources6
Helixi471 – 482Combined sources12
Helixi483 – 485Combined sources3
Beta strandi486 – 490Combined sources5
Beta strandi492 – 500Combined sources9
Beta strandi502 – 504Combined sources3
Beta strandi508 – 511Combined sources4
Helixi512 – 522Combined sources11
Helixi524 – 543Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3AX1X-ray2.74A194-543[»]
ProteinModelPortaliQ9ZVD0.
SMRiQ9ZVD0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9ZVD0.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi25 – 111Pro-richAdd BLAST87
Compositional biasi121 – 182Gly-richAdd BLAST62
Compositional biasi562 – 598Arg-richAdd BLAST37
Compositional biasi629 – 690Pro-richAdd BLAST62

Sequence similaritiesi

Belongs to the ARS2 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri498 – 523C2H2-typeAdd BLAST26

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2295. Eukaryota.
ENOG410XR8S. LUCA.
HOGENOMiHOG000082562.
InParanoidiQ9ZVD0.
OMAiDNGYDAR.
OrthoDBiEOG0936071Q.
PhylomeDBiQ9ZVD0.

Family and domain databases

InterProiView protein in InterPro
IPR007042. Arsenite-R_2.
IPR021933. DUF3546.
IPR013087. Znf_C2H2_type.
PfamiView protein in Pfam
PF04959. ARS2. 1 hit.
PF12066. DUF3546. 1 hit.
PROSITEiView protein in PROSITE
PS00028. ZINC_FINGER_C2H2_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9ZVD0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADVNLPPSD SVDNRLPEKS TSSSPPPPPP SSSLPQQEQE QDQQQLPLRR
60 70 80 90 100
ERDSRERRDE RDIERPPPNR RERDRSPLPP PRRDYKRRPS LSPPPPYRDR
110 120 130 140 150
RHSPPQRRSP PQKRYRRDDN GYDGRRGSPR GGYGPPDRRF GYDHGGGYDR
160 170 180 190 200
EMGGRPGYGD ERPHGRFMGR YQDWEGGRGG YGDASNSGNP QRDGLMSYKQ
210 220 230 240 250
FIQELEDDIL PSEAERRYQE YKSEYITTQK RAFFNTHKEE DWLKNKYHPT
260 270 280 290 300
NLLSVIERRN DLAQKVAKDF LLDLQSGTLD LGPAVTALNK SGRTSEPNSE
310 320 330 340 350
DEAAGVGKRK RHGMGGAKEN ELLSAAPKAP SFTSDPKRIL TDVEQTQALV
360 370 380 390 400
RKLDSEKKIE ENVLQGSETE KSGREKLHSG STGPVVIIRG LTSVKGLEGV
410 420 430 440 450
ELLDTLVTYL WRVHGLDYYG KVETNEAKGL RHVRAEGKVS DAKGDENESK
460 470 480 490 500
FDSHWQERLK GQDPLEVMAA KEKIDAAATE ALDPHVRKIR DEKYGWKYGC
510 520 530 540 550
GAKGCTKLFH AAEFVYKHLK LKHTELVTEL TTKVREELYF QNYMNDPNAP
560 570 580 590 600
GGQPATQQSG PRDRPIRRKP SMENRLRDDR GGRRERDGRA NGNDRNDRSE
610 620 630 640 650
DQQRGDNDGG NPGEVGYDAF GGQGGVHVPP FLSDINPPPM LMPVPGAGPL
660 670 680 690 700
GPFVPAPPEV AMQMFRDPSG PNPPFEGSGR GGPAPFLLSP AFRQDPRRLR
710 720
SYQDLDAPEE EVTVIDYRSL
Length:720
Mass (Da):81,098
Last modified:June 1, 2002 - v2
Checksum:i44D7041FAEA4A857
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF311221 mRNA. Translation: AAK63206.1.
AC005623 Genomic DNA. Translation: AAC77868.2.
CP002685 Genomic DNA. Translation: AEC07936.1.
AF428305 mRNA. Translation: AAL16137.1.
AY039915 mRNA. Translation: AAK64019.1.
AY142569 mRNA. Translation: AAN13138.1.
AY143937 mRNA. Translation: AAN28876.1.
PIRiG84668.
RefSeqiNP_565635.1. NM_128268.3.
UniGeneiAt.25511.

Genome annotation databases

EnsemblPlantsiAT2G27100.1; AT2G27100.1; AT2G27100.
GeneIDi817252.
GrameneiAT2G27100.1; AT2G27100.1; AT2G27100.
KEGGiath:AT2G27100.

Similar proteinsi

Entry informationi

Entry nameiSRRT_ARATH
AccessioniPrimary (citable) accession number: Q9ZVD0
Secondary accession number(s): Q93W84
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 1, 2002
Last modified: January 31, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families