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Protein

Regulatory protein NPR5

Gene

NPR5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). Acts redundantly with BOP2. BOP1/2 promote leaf and floral meristem fate and determinacy in a pathway targeting AP1 and AGL24. BOP1/2 act as transcriptional co-regulators through direct interaction with TGA factors, including PAN, a direct regulator of AP1. Controls lateral organ fate through positive regulation of adaxial-abaxial polarity genes ATHB-14/PHB, YAB1/FIL and YAB3, and through positive regulation of LOB domain-containing genes LOB, LBD6/AS2 and LBD36. Promotes and maintains a developmentally determinate state in leaf cells through the negative regulation of JAG, JGL and class I KNOX genes. Is also involved in nectary development, formation of normal abscission zones and suppression of bract formation.By similarity7 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • bract formation Source: UniProtKB
  • floral meristem determinacy Source: TAIR
  • floral organ abscission Source: UniProtKB
  • flower morphogenesis Source: TAIR
  • induced systemic resistance, jasmonic acid mediated signaling pathway Source: TAIR
  • nectary development Source: TAIR
  • polarity specification of adaxial/abaxial axis Source: TAIR
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: GO_Central
  • proximal/distal pattern formation Source: TAIR
  • regulation of proteolysis Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulatory protein NPR5
Alternative name(s):
BTB/POZ domain-containing protein NPR5
Protein BLADE ON PETIOLE 2
Gene namesi
Name:NPR5
Synonyms:BOP2
Ordered Locus Names:At2g41370
ORF Names:F13H10.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G41370.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • nucleus Source: TAIR
  • SCF ubiquitin ligase complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Defects in inflorescence and flower development. Bop1 and bop2 double mutant displays leafy petioles, loss of floral organ abscission, and asymmetric flowers subtended by a bract.4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004079941 – 491Regulatory protein NPR5Add BLAST491

Proteomic databases

PaxDbiQ9ZVC2.

PTM databases

iPTMnetiQ9ZVC2.

Expressioni

Tissue specificityi

Highly expressed in young floral meristem.1 Publication

Developmental stagei

Expressed during vegetative development in young leaf primordia and at the base of the rosette leaves on the adaxial side. Expressed during reproductive development in young floral buds, and at the base of the sepals and petals.1 Publication

Gene expression databases

GenevisibleiQ9ZVC2. AT.

Interactioni

Subunit structurei

Homodimer or heterodimer with BOP1. Interacts with PAN.2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4072. 5 interactors.
IntActiQ9ZVC2. 4 interactors.
STRINGi3702.AT2G41370.1.

Structurei

3D structure databases

ProteinModelPortaliQ9ZVC2.
SMRiQ9ZVC2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 116BTBPROSITE-ProRule annotationAdd BLAST91
Repeati254 – 283ANK 1Add BLAST30
Repeati284 – 313ANK 2Add BLAST30
Repeati318 – 347ANK 3Add BLAST30

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi403 – 435Asn-richAdd BLAST33
Compositional biasi472 – 490His-richAdd BLAST19

Domaini

The BTB/POZ domain mediates the interaction with some component of ubiquitin ligase complexes.1 Publication

Sequence similaritiesi

Contains 3 ANK repeats.PROSITE-ProRule annotation
Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
HOGENOMiHOG000234377.
InParanoidiQ9ZVC2.
KOiK14508.
OMAiIFQPINN.
OrthoDBiEOG093609PR.
PhylomeDBiQ9ZVC2.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR000210. BTB/POZ_dom.
IPR024228. NPR_central_dom.
IPR011333. SKP1/BTB/POZ.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00651. BTB. 1 hit.
PF11900. DUF3420. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
SM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF54695. SSF54695. 2 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50097. BTB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZVC2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNLEESLRS LSLDFLNLLI NGQAFSDVTF SVEGRLVHAH RCILAARSLF
60 70 80 90 100
FRKFFCGTDS PQPVTGIDPT QHGSVPASPT RGSTAPAGII PVNSVGYEVF
110 120 130 140 150
LLLLQFLYSG QVSIVPQKHE PRPNCGERGC WHTHCSAAVD LALDTLAASR
160 170 180 190 200
YFGVEQLALL TQKQLASMVE KASIEDVMKV LIASRKQDMH QLWTTCSHLV
210 220 230 240 250
AKSGLPPEIL AKHLPIDVVT KIEELRLKSS IARRSLMPHN HHHDLSVAQD
260 270 280 290 300
LEDQKIRRMR RALDSSDVEL VKLMVMGEGL NLDESLALHY AVESCSREVV
310 320 330 340 350
KALLELGAAD VNYPAGPAGK TPLHIAAEMV SPDMVAVLLD HHADPNVRTV
360 370 380 390 400
GGITPLDILR TLTSDFLFKG AVPGLTHIEP NKLRLCLELV QSAAMVISRE
410 420 430 440 450
EGNNSNNQNN DNNTGIYPHM NEEHNSGSSG GSNNNLDSRL VYLNLGAGTG
460 470 480 490
QMGPGRDQGD DHNSQREGMS RHHHHHQDPS TMYHHHHQHH F
Length:491
Mass (Da):54,001
Last modified:May 1, 1999 - v1
Checksum:iF2E8A7133B591776
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti386C → R in AAO22795 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY928830 mRNA. Translation: AAX22759.1.
AC005662 Genomic DNA. Translation: AAC78536.1.
CP002685 Genomic DNA. Translation: AEC09966.1.
BT002986 mRNA. Translation: AAO22795.1.
BT015773 mRNA. Translation: AAU90063.1.
PIRiH84840.
RefSeqiNP_181668.1. NM_129700.3.
UniGeneiAt.43967.

Genome annotation databases

EnsemblPlantsiAT2G41370.1; AT2G41370.1; AT2G41370.
GeneIDi818735.
GrameneiAT2G41370.1; AT2G41370.1; AT2G41370.
KEGGiath:AT2G41370.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY928830 mRNA. Translation: AAX22759.1.
AC005662 Genomic DNA. Translation: AAC78536.1.
CP002685 Genomic DNA. Translation: AEC09966.1.
BT002986 mRNA. Translation: AAO22795.1.
BT015773 mRNA. Translation: AAU90063.1.
PIRiH84840.
RefSeqiNP_181668.1. NM_129700.3.
UniGeneiAt.43967.

3D structure databases

ProteinModelPortaliQ9ZVC2.
SMRiQ9ZVC2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4072. 5 interactors.
IntActiQ9ZVC2. 4 interactors.
STRINGi3702.AT2G41370.1.

PTM databases

iPTMnetiQ9ZVC2.

Proteomic databases

PaxDbiQ9ZVC2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G41370.1; AT2G41370.1; AT2G41370.
GeneIDi818735.
GrameneiAT2G41370.1; AT2G41370.1; AT2G41370.
KEGGiath:AT2G41370.

Organism-specific databases

TAIRiAT2G41370.

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
HOGENOMiHOG000234377.
InParanoidiQ9ZVC2.
KOiK14508.
OMAiIFQPINN.
OrthoDBiEOG093609PR.
PhylomeDBiQ9ZVC2.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ9ZVC2.

Gene expression databases

GenevisibleiQ9ZVC2. AT.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR000210. BTB/POZ_dom.
IPR024228. NPR_central_dom.
IPR011333. SKP1/BTB/POZ.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00651. BTB. 1 hit.
PF11900. DUF3420. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
SM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF54695. SSF54695. 2 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50097. BTB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNPR5_ARATH
AccessioniPrimary (citable) accession number: Q9ZVC2
Secondary accession number(s): Q84WN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.