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Protein

Regulatory protein NPR5

Gene

NPR5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). Acts redundantly with BOP2. BOP1/2 promote leaf and floral meristem fate and determinacy in a pathway targeting AP1 and AGL24. BOP1/2 act as transcriptional co-regulators through direct interaction with TGA factors, including PAN, a direct regulator of AP1. Controls lateral organ fate through positive regulation of adaxial-abaxial polarity genes ATHB-14/PHB, YAB1/FIL and YAB3, and through positive regulation of LOB domain-containing genes LOB, LBD6/AS2 and LBD36. Promotes and maintains a developmentally determinate state in leaf cells through the negative regulation of JAG, JGL and class I KNOX genes. Is also involved in nectary development, formation of normal abscission zones and suppression of bract formation.By similarity7 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • bract formation Source: UniProtKB
  • floral meristem determinacy Source: TAIR
  • floral organ abscission Source: UniProtKB
  • flower morphogenesis Source: TAIR
  • induced systemic resistance, jasmonic acid mediated signaling pathway Source: TAIR
  • nectary development Source: TAIR
  • polarity specification of adaxial/abaxial axis Source: TAIR
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
  • protein ubiquitination Source: UniProtKB-UniPathway
  • proximal/distal pattern formation Source: TAIR
  • regulation of proteolysis Source: GO_Central

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Regulatory protein NPR5
Alternative name(s):
BTB/POZ domain-containing protein NPR5
Protein BLADE ON PETIOLE 2
Gene namesi
Name:NPR5
Synonyms:BOP2
Ordered Locus Names:At2g41370
ORF Names:F13H10.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G41370
TAIRilocus:2040292 AT2G41370

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Defects in inflorescence and flower development. Bop1 and bop2 double mutant displays leafy petioles, loss of floral organ abscission, and asymmetric flowers subtended by a bract.4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004079941 – 491Regulatory protein NPR5Add BLAST491

Proteomic databases

PaxDbiQ9ZVC2

PTM databases

iPTMnetiQ9ZVC2

Expressioni

Tissue specificityi

Highly expressed in young floral meristem.1 Publication

Developmental stagei

Expressed during vegetative development in young leaf primordia and at the base of the rosette leaves on the adaxial side. Expressed during reproductive development in young floral buds, and at the base of the sepals and petals.1 Publication

Gene expression databases

ExpressionAtlasiQ9ZVC2 baseline and differential
GenevisibleiQ9ZVC2 AT

Interactioni

Subunit structurei

Homodimer or heterodimer with BOP1. Interacts with PAN.2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4072, 5 interactors
IntActiQ9ZVC2, 4 interactors
STRINGi3702.AT2G41370.1

Structurei

3D structure databases

ProteinModelPortaliQ9ZVC2
SMRiQ9ZVC2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 116BTBPROSITE-ProRule annotationAdd BLAST91
Repeati254 – 283ANK 1Add BLAST30
Repeati284 – 313ANK 2Add BLAST30
Repeati318 – 347ANK 3Add BLAST30

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi403 – 435Asn-richAdd BLAST33
Compositional biasi472 – 490His-richAdd BLAST19

Domaini

The BTB/POZ domain mediates the interaction with some component of ubiquitin ligase complexes.1 Publication

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0504 Eukaryota
COG0666 LUCA
HOGENOMiHOG000234377
InParanoidiQ9ZVC2
KOiK14508
OMAiNTGIYPH
OrthoDBiEOG093609PR
PhylomeDBiQ9ZVC2

Family and domain databases

CDDicd00204 ANK, 1 hit
Gene3Di1.25.40.20, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR000210 BTB/POZ_dom
IPR024228 NPR_central_dom
IPR011333 SKP1/BTB/POZ_sf
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF00651 BTB, 1 hit
PF11900 DUF3420, 1 hit
SMARTiView protein in SMART
SM00248 ANK, 2 hits
SM00225 BTB, 1 hit
SUPFAMiSSF48403 SSF48403, 1 hit
SSF54695 SSF54695, 2 hits
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 1 hit
PS50097 BTB, 1 hit

Sequencei

Sequence statusi: Complete.

Q9ZVC2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNLEESLRS LSLDFLNLLI NGQAFSDVTF SVEGRLVHAH RCILAARSLF
60 70 80 90 100
FRKFFCGTDS PQPVTGIDPT QHGSVPASPT RGSTAPAGII PVNSVGYEVF
110 120 130 140 150
LLLLQFLYSG QVSIVPQKHE PRPNCGERGC WHTHCSAAVD LALDTLAASR
160 170 180 190 200
YFGVEQLALL TQKQLASMVE KASIEDVMKV LIASRKQDMH QLWTTCSHLV
210 220 230 240 250
AKSGLPPEIL AKHLPIDVVT KIEELRLKSS IARRSLMPHN HHHDLSVAQD
260 270 280 290 300
LEDQKIRRMR RALDSSDVEL VKLMVMGEGL NLDESLALHY AVESCSREVV
310 320 330 340 350
KALLELGAAD VNYPAGPAGK TPLHIAAEMV SPDMVAVLLD HHADPNVRTV
360 370 380 390 400
GGITPLDILR TLTSDFLFKG AVPGLTHIEP NKLRLCLELV QSAAMVISRE
410 420 430 440 450
EGNNSNNQNN DNNTGIYPHM NEEHNSGSSG GSNNNLDSRL VYLNLGAGTG
460 470 480 490
QMGPGRDQGD DHNSQREGMS RHHHHHQDPS TMYHHHHQHH F
Length:491
Mass (Da):54,001
Last modified:May 1, 1999 - v1
Checksum:iF2E8A7133B591776
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti386C → R in AAO22795 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY928830 mRNA Translation: AAX22759.1
AC005662 Genomic DNA Translation: AAC78536.1
CP002685 Genomic DNA Translation: AEC09966.1
BT002986 mRNA Translation: AAO22795.1
BT015773 mRNA Translation: AAU90063.1
PIRiH84840
RefSeqiNP_181668.1, NM_129700.3
UniGeneiAt.43967

Genome annotation databases

EnsemblPlantsiAT2G41370.1; AT2G41370.1; AT2G41370
GeneIDi818735
GrameneiAT2G41370.1; AT2G41370.1; AT2G41370
KEGGiath:AT2G41370

Similar proteinsi

Entry informationi

Entry nameiNPR5_ARATH
AccessioniPrimary (citable) accession number: Q9ZVC2
Secondary accession number(s): Q84WN1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: May 1, 1999
Last modified: April 25, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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