Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9ZVC2

- NPR5_ARATH

UniProt

Q9ZVC2 - NPR5_ARATH

Protein

Regulatory protein NPR5

Gene

NPR5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins By similarity. Acts redundantly with BOP2. BOP1/2 promote leaf and floral meristem fate and determinacy in a pathway targeting AP1 and AGL24. BOP1/2 act as transcriptional co-regulators through direct interaction with TGA factors, including PAN, a direct regulator of AP1. Controls lateral organ fate through positive regulation of adaxial-abaxial polarity genes ATHB-14/PHB, YAB1/FIL and YAB3, and through positive regulation of LOB domain-containing genes LOB, LBD6/AS2 and LBD36. Promotes and maintains a developmentally determinate state in leaf cells through the negative regulation of JAG, JGL and class I KNOX genes. Is also involved in nectary development, formation of normal abscission zones and suppression of bract formation.By similarity7 Publications

    Pathwayi

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    GO - Biological processi

    1. bract formation Source: UniProtKB
    2. floral meristem determinacy Source: TAIR
    3. floral organ abscission Source: UniProtKB
    4. flower morphogenesis Source: TAIR
    5. induced systemic resistance, jasmonic acid mediated signaling pathway Source: TAIR
    6. nectary development Source: TAIR
    7. polarity specification of adaxial/abaxial axis Source: TAIR
    8. protein ubiquitination Source: UniProtKB-UniPathway
    9. proximal/distal pattern formation Source: TAIR

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Regulatory protein NPR5
    Alternative name(s):
    BTB/POZ domain-containing protein NPR5
    Protein BLADE ON PETIOLE 2
    Gene namesi
    Name:NPR5
    Synonyms:BOP2
    Ordered Locus Names:At2g41370
    ORF Names:F13H10.8
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G41370.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: TAIR
    2. nucleus Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Defects in inflorescence and flower development. Bop1 and bop2 double mutant displays leafy petioles, loss of floral organ abscission, and asymmetric flowers subtended by a bract.4 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 491491Regulatory protein NPR5PRO_0000407994Add
    BLAST

    Proteomic databases

    PaxDbiQ9ZVC2.
    PRIDEiQ9ZVC2.

    Expressioni

    Tissue specificityi

    Highly expressed in young floral meristem.1 Publication

    Developmental stagei

    Expressed during vegetative development in young leaf primordia and at the base of the rosette leaves on the adaxial side. Expressed during reproductive development in young floral buds, and at the base of the sepals and petals.1 Publication

    Gene expression databases

    GenevestigatoriQ9ZVC2.

    Interactioni

    Subunit structurei

    Homodimer or heterodimer with BOP1. Interacts with PAN.2 Publications

    Protein-protein interaction databases

    BioGridi4072. 5 interactions.
    IntActiQ9ZVC2. 4 interactions.
    STRINGi3702.AT2G41370.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ZVC2.
    SMRiQ9ZVC2. Positions 203-423.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini26 – 11691BTBPROSITE-ProRule annotationAdd
    BLAST
    Repeati254 – 28330ANK 1Add
    BLAST
    Repeati284 – 31330ANK 2Add
    BLAST
    Repeati318 – 34730ANK 3Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi403 – 43533Asn-richAdd
    BLAST
    Compositional biasi472 – 49019His-richAdd
    BLAST

    Domaini

    The BTB/POZ domain mediates the interaction with some component of ubiquitin ligase complexes.1 Publication

    Sequence similaritiesi

    Contains 3 ANK repeats.PROSITE-ProRule annotation
    Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    ANK repeat, Repeat

    Phylogenomic databases

    eggNOGiNOG298720.
    HOGENOMiHOG000234377.
    InParanoidiQ9ZVC2.
    KOiK14508.
    OMAiHYAVESC.
    PhylomeDBiQ9ZVC2.

    Family and domain databases

    Gene3Di1.25.40.20. 1 hit.
    3.30.710.10. 1 hit.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR013069. BTB_POZ.
    IPR024228. DUF3420.
    [Graphical view]
    PfamiPF00023. Ank. 2 hits.
    PF00651. BTB. 1 hit.
    PF11900. DUF3420. 1 hit.
    [Graphical view]
    SMARTiSM00248. ANK. 2 hits.
    SM00225. BTB. 1 hit.
    [Graphical view]
    SUPFAMiSSF48403. SSF48403. 1 hit.
    SSF54695. SSF54695. 2 hits.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 1 hit.
    PS50097. BTB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9ZVC2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNLEESLRS LSLDFLNLLI NGQAFSDVTF SVEGRLVHAH RCILAARSLF    50
    FRKFFCGTDS PQPVTGIDPT QHGSVPASPT RGSTAPAGII PVNSVGYEVF 100
    LLLLQFLYSG QVSIVPQKHE PRPNCGERGC WHTHCSAAVD LALDTLAASR 150
    YFGVEQLALL TQKQLASMVE KASIEDVMKV LIASRKQDMH QLWTTCSHLV 200
    AKSGLPPEIL AKHLPIDVVT KIEELRLKSS IARRSLMPHN HHHDLSVAQD 250
    LEDQKIRRMR RALDSSDVEL VKLMVMGEGL NLDESLALHY AVESCSREVV 300
    KALLELGAAD VNYPAGPAGK TPLHIAAEMV SPDMVAVLLD HHADPNVRTV 350
    GGITPLDILR TLTSDFLFKG AVPGLTHIEP NKLRLCLELV QSAAMVISRE 400
    EGNNSNNQNN DNNTGIYPHM NEEHNSGSSG GSNNNLDSRL VYLNLGAGTG 450
    QMGPGRDQGD DHNSQREGMS RHHHHHQDPS TMYHHHHQHH F 491
    Length:491
    Mass (Da):54,001
    Last modified:May 1, 1999 - v1
    Checksum:iF2E8A7133B591776
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti386 – 3861C → R in AAO22795. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY928830 mRNA. Translation: AAX22759.1.
    AC005662 Genomic DNA. Translation: AAC78536.1.
    CP002685 Genomic DNA. Translation: AEC09966.1.
    BT002986 mRNA. Translation: AAO22795.1.
    BT015773 mRNA. Translation: AAU90063.1.
    PIRiH84840.
    RefSeqiNP_181668.1. NM_129700.2.
    UniGeneiAt.43967.

    Genome annotation databases

    EnsemblPlantsiAT2G41370.1; AT2G41370.1; AT2G41370.
    GeneIDi818735.
    KEGGiath:AT2G41370.

    Cross-referencesi

    Web resourcesi

    PlantsUBQ

    A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY928830 mRNA. Translation: AAX22759.1 .
    AC005662 Genomic DNA. Translation: AAC78536.1 .
    CP002685 Genomic DNA. Translation: AEC09966.1 .
    BT002986 mRNA. Translation: AAO22795.1 .
    BT015773 mRNA. Translation: AAU90063.1 .
    PIRi H84840.
    RefSeqi NP_181668.1. NM_129700.2.
    UniGenei At.43967.

    3D structure databases

    ProteinModelPortali Q9ZVC2.
    SMRi Q9ZVC2. Positions 203-423.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 4072. 5 interactions.
    IntActi Q9ZVC2. 4 interactions.
    STRINGi 3702.AT2G41370.1-P.

    Proteomic databases

    PaxDbi Q9ZVC2.
    PRIDEi Q9ZVC2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G41370.1 ; AT2G41370.1 ; AT2G41370 .
    GeneIDi 818735.
    KEGGi ath:AT2G41370.

    Organism-specific databases

    TAIRi AT2G41370.

    Phylogenomic databases

    eggNOGi NOG298720.
    HOGENOMi HOG000234377.
    InParanoidi Q9ZVC2.
    KOi K14508.
    OMAi HYAVESC.
    PhylomeDBi Q9ZVC2.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Gene expression databases

    Genevestigatori Q9ZVC2.

    Family and domain databases

    Gene3Di 1.25.40.20. 1 hit.
    3.30.710.10. 1 hit.
    InterProi IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR013069. BTB_POZ.
    IPR024228. DUF3420.
    [Graphical view ]
    Pfami PF00023. Ank. 2 hits.
    PF00651. BTB. 1 hit.
    PF11900. DUF3420. 1 hit.
    [Graphical view ]
    SMARTi SM00248. ANK. 2 hits.
    SM00225. BTB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48403. SSF48403. 1 hit.
    SSF54695. SSF54695. 2 hits.
    PROSITEi PS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 1 hit.
    PS50097. BTB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "BLADE-ON-PETIOLE-dependent signaling controls leaf and floral patterning in Arabidopsis."
      Hepworth S.R., Zhang Y., McKim S., Li X., Haughn G.W.
      Plant Cell 17:1434-1448(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH PAN.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Arabidopsis ORF clones."
      Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.
      Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "The BLADE ON PETIOLE genes act redundantly to control the growth and development of lateral organs."
      Norberg M., Holmlund M., Nilsson O.
      Development 132:2203-2213(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.
    7. "Cullins 3a and 3b assemble with members of the broad complex/tramtrack/bric-a-brac (BTB) protein family to form essential ubiquitin-protein ligases (E3s) in Arabidopsis."
      Gingerich D.J., Gagne J.M., Salter D.W., Hellmann H., Estelle M., Ma L., Vierstra R.D.
      J. Biol. Chem. 280:18810-18821(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: DOMAIN BTB.
    8. "An Arabidopsis NPR1-like gene, NPR4, is required for disease resistance."
      Liu G., Holub E.B., Alonso J.M., Ecker J.R., Fobert P.R.
      Plant J. 41:304-318(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    9. "BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes."
      Ha C.M., Jun J.H., Nam H.G., Fletcher J.C.
      Plant Cell 19:1809-1825(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    10. "The BLADE-ON-PETIOLE genes are essential for abscission zone formation in Arabidopsis."
      McKim S.M., Stenvik G.E., Butenko M.A., Kristiansen W., Cho S.K., Hepworth S.R., Aalen R.B., Haughn G.W.
      Development 135:1537-1546(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    11. "Control of Arabidopsis leaf morphogenesis through regulation of the YABBY and KNOX families of transcription factors."
      Ha C.M., Jun J.H., Fletcher J.C.
      Genetics 186:197-206(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "BLADE-ON-PETIOLE1 coordinates organ determinacy and axial polarity in arabidopsis by directly activating ASYMMETRIC LEAVES2."
      Jun J.H., Ha C.M., Fletcher J.C.
      Plant Cell 22:62-76(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.
    13. "Arabidopsis BLADE-ON-PETIOLE1 and 2 promote floral meristem fate and determinacy in a previously undefined pathway targeting APETALA1 and AGAMOUS-LIKE24."
      Xu M., Hu T., McKim S.M., Murmu J., Haughn G.W., Hepworth S.R.
      Plant J. 63:974-989(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiNPR5_ARATH
    AccessioniPrimary (citable) accession number: Q9ZVC2
    Secondary accession number(s): Q84WN1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3