Regulatory protein NPR5 - Arabidopsis thaliana (Mouse-ear cress)

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Q9ZVC2 (NPR5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 88. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Regulatory protein NPR5

Alternative name(s):
BTB/POZ domain-containing protein NPR5
Protein BLADE ON PETIOLE 2

Gene names

Name:	NPR5
Synonyms:	BOP2
Ordered Locus Names:	At2g41370
ORF Names:	F13H10.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins By similarity. Acts redundantly with BOP2. BOP1/2 promote leaf and floral meristem fate and determinacy in a pathway targeting AP1 and AGL24. BOP1/2 act as transcriptional co-regulators through direct interaction with TGA factors, including PAN, a direct regulator of AP1. Controls lateral organ fate through positive regulation of adaxial-abaxial polarity genes ATHB-14/PHB, YAB1/FIL and YAB3, and through positive regulation of LOB domain-containing genes LOB, LBD6/AS2 and LBD36. Promotes and maintains a developmentally determinate state in leaf cells through the negative regulation of JAG, JGL and class I KNOX genes. Is also involved in nectary development, formation of normal abscission zones and suppression of bract formation. Ref.1 Ref.6 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Homodimer or heterodimer with BOP1. Interacts with PAN. Ref.1 Ref.12
Subcellular location	Cytoplasm. Nucleus Ref.1.
Tissue specificity	Highly expressed in young floral meristem. Ref.1
Developmental stage	Expressed during vegetative development in young leaf primordia and at the base of the rosette leaves on the adaxial side. Expressed during reproductive development in young floral buds, and at the base of the sepals and petals. Ref.6
Domain	The BTB/POZ domain mediates the interaction with some component of ubiquitin ligase complexes. Ref.7
Disruption phenotype	Defects in inflorescence and flower development. Double mutant bop1bop2 displays leafy petioles, loss of floral organ abscission, and asymmetric flowers subtended by a bract. Ref.1 Ref.6 Ref.9 Ref.10
Sequence similarities	Contains 3 ANK repeats. Contains 1 BTB (POZ) domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Domain	ANK repeat Repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	bract formation Inferred from genetic interaction Ref.6. Source: UniProtKB floral meristem determinacy Inferred from genetic interaction Ref.13. Source: TAIR floral organ abscission Inferred from genetic interaction Ref.6. Source: UniProtKB flower morphogenesis Inferred from genetic interaction Ref.1. Source: TAIR induced systemic resistance, jasmonic acid mediated signaling pathway Inferred from genetic interaction PubMed 23116333. Source: TAIR nectary development Inferred from genetic interaction Ref.10. Source: TAIR polarity specification of adaxial/abaxial axis Inferred from genetic interaction Ref.9. Source: TAIR protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-UniPathway proximal/distal pattern formation Inferred from genetic interaction Ref.1. Source: TAIR
Cellular_component	cytoplasm Inferred from direct assay Ref.1. Source: TAIR nucleus Inferred from direct assay Ref.1. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 491

491

Regulatory protein NPR5

PRO_0000407994

Regions

Domain

26 – 116

BTB

Repeat

254 – 283

ANK 1

Repeat

284 – 313

ANK 2

Repeat

318 – 347

ANK 3

Compositional bias

403 – 435

Asn-rich

Compositional bias

472 – 490

His-rich

Experimental info

Sequence conflict

386

C → R in AAO22795. Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

Q9ZVC2 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: F2E8A7133B591776
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FASTA

491

54,001

        10         20         30         40         50         60 
MSNLEESLRS LSLDFLNLLI NGQAFSDVTF SVEGRLVHAH RCILAARSLF FRKFFCGTDS 

        70         80         90        100        110        120 
PQPVTGIDPT QHGSVPASPT RGSTAPAGII PVNSVGYEVF LLLLQFLYSG QVSIVPQKHE 

       130        140        150        160        170        180 
PRPNCGERGC WHTHCSAAVD LALDTLAASR YFGVEQLALL TQKQLASMVE KASIEDVMKV 

       190        200        210        220        230        240 
LIASRKQDMH QLWTTCSHLV AKSGLPPEIL AKHLPIDVVT KIEELRLKSS IARRSLMPHN 

       250        260        270        280        290        300 
HHHDLSVAQD LEDQKIRRMR RALDSSDVEL VKLMVMGEGL NLDESLALHY AVESCSREVV 

       310        320        330        340        350        360 
KALLELGAAD VNYPAGPAGK TPLHIAAEMV SPDMVAVLLD HHADPNVRTV GGITPLDILR 

       370        380        390        400        410        420 
TLTSDFLFKG AVPGLTHIEP NKLRLCLELV QSAAMVISRE EGNNSNNQNN DNNTGIYPHM 

       430        440        450        460        470        480 
NEEHNSGSSG GSNNNLDSRL VYLNLGAGTG QMGPGRDQGD DHNSQREGMS RHHHHHQDPS 

       490 
TMYHHHHQHH F

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"BLADE-ON-PETIOLE-dependent signaling controls leaf and floral patterning in Arabidopsis." Hepworth S.R., Zhang Y., McKim S., Li X., Haughn G.W. Plant Cell 17:1434-1448(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH PAN.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Arabidopsis ORF clones." Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"The BLADE ON PETIOLE genes act redundantly to control the growth and development of lateral organs." Norberg M., Holmlund M., Nilsson O. Development 132:2203-2213(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.
[7]	"Cullins 3a and 3b assemble with members of the broad complex/tramtrack/bric-a-brac (BTB) protein family to form essential ubiquitin-protein ligases (E3s) in Arabidopsis." Gingerich D.J., Gagne J.M., Salter D.W., Hellmann H., Estelle M., Ma L., Vierstra R.D. J. Biol. Chem. 280:18810-18821(2005) [PubMed] [Europe PMC] [Abstract] Cited for: DOMAIN BTB.
[8]	"An Arabidopsis NPR1-like gene, NPR4, is required for disease resistance." Liu G., Holub E.B., Alonso J.M., Ecker J.R., Fobert P.R. Plant J. 41:304-318(2005) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[9]	"BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes." Ha C.M., Jun J.H., Nam H.G., Fletcher J.C. Plant Cell 19:1809-1825(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[10]	"The BLADE-ON-PETIOLE genes are essential for abscission zone formation in Arabidopsis." McKim S.M., Stenvik G.E., Butenko M.A., Kristiansen W., Cho S.K., Hepworth S.R., Aalen R.B., Haughn G.W. Development 135:1537-1546(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[11]	"Control of Arabidopsis leaf morphogenesis through regulation of the YABBY and KNOX families of transcription factors." Ha C.M., Jun J.H., Fletcher J.C. Genetics 186:197-206(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[12]	"BLADE-ON-PETIOLE1 coordinates organ determinacy and axial polarity in arabidopsis by directly activating ASYMMETRIC LEAVES2." Jun J.H., Ha C.M., Fletcher J.C. Plant Cell 22:62-76(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT.
[13]	"Arabidopsis BLADE-ON-PETIOLE1 and 2 promote floral meristem fate and determinacy in a previously undefined pathway targeting APETALA1 and AGAMOUS-LIKE24." Xu M., Hu T., McKim S.M., Murmu J., Haughn G.W., Hepworth S.R. Plant J. 63:974-989(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY928830 mRNA. Translation: AAX22759.1. AC005662 Genomic DNA. Translation: AAC78536.1. CP002685 Genomic DNA. Translation: AEC09966.1. BT002986 mRNA. Translation: AAO22795.1. BT015773 mRNA. Translation: AAU90063.1.
IPI	IPI00522016.
PIR	H84840.
RefSeq	NP_181668.1. NM_129700.2.
UniGene	At.43967.
3D structure databases
HSSP	HSSP built from PDB template 1OY3 based on UniProtKB Q60778.
ProteinModelPortal	Q9ZVC2.
SMR	Q9ZVC2. Positions 203-423.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9ZVC2. 4 interactions.
STRING	3702.AT2G41370.1-P.
Proteomic databases
PaxDb	Q9ZVC2.
PRIDE	Q9ZVC2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G41370.1; AT2G41370.1; AT2G41370.
GeneID	818735.
KEGG	ath:AT2G41370.
Organism-specific databases
TAIR	At2g41370.
Phylogenomic databases
eggNOG	NOG298720.
HOGENOM	HOG000234377.
InParanoid	Q9ZVC2.
KO	K14508.
OMA	HYAVESC.
PhylomeDB	Q9ZVC2.
ProtClustDB	CLSN2683770.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Genevestigator	Q9ZVC2.
Family and domain databases
Gene3D	1.25.40.20. 1 hit. 3.30.710.10. 1 hit.
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR000210. BTB/POZ-like. IPR011333. BTB/POZ_fold. IPR013069. BTB_POZ. IPR024228. DUF3420. [Graphical view]
Pfam	PF00023. Ank. 2 hits. PF00651. BTB. 1 hit. PF11900. DUF3420. 1 hit. [Graphical view]
SMART	SM00248. ANK. 2 hits. SM00225. BTB. 1 hit. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit. SSF54695. BTB/POZ_fold. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 1 hit. PS50097. BTB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NPR5_ARATH

Accession

Primary (citable) accession number: Q9ZVC2
Secondary accession number(s): Q84WN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 3, 2011
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Web resources

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents