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Q9ZVC2 (NPR5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Regulatory protein NPR5
Alternative name(s):
BTB/POZ domain-containing protein NPR5
Protein BLADE ON PETIOLE 2
Gene names
Name:NPR5
Synonyms:BOP2
Ordered Locus Names:At2g41370
ORF Names:F13H10.8
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length491 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins By similarity. Acts redundantly with BOP2. BOP1/2 promote leaf and floral meristem fate and determinacy in a pathway targeting AP1 and AGL24. BOP1/2 act as transcriptional co-regulators through direct interaction with TGA factors, including PAN, a direct regulator of AP1. Controls lateral organ fate through positive regulation of adaxial-abaxial polarity genes ATHB-14/PHB, YAB1/FIL and YAB3, and through positive regulation of LOB domain-containing genes LOB, LBD6/AS2 and LBD36. Promotes and maintains a developmentally determinate state in leaf cells through the negative regulation of JAG, JGL and class I KNOX genes. Is also involved in nectary development, formation of normal abscission zones and suppression of bract formation. Ref.1 Ref.6 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Homodimer or heterodimer with BOP1. Interacts with PAN. Ref.1 Ref.12

Subcellular location

Cytoplasm. Nucleus Ref.1.

Tissue specificity

Highly expressed in young floral meristem. Ref.1

Developmental stage

Expressed during vegetative development in young leaf primordia and at the base of the rosette leaves on the adaxial side. Expressed during reproductive development in young floral buds, and at the base of the sepals and petals. Ref.6

Domain

The BTB/POZ domain mediates the interaction with some component of ubiquitin ligase complexes. Ref.7

Disruption phenotype

Defects in inflorescence and flower development. Bop1 and bop2 double mutant displays leafy petioles, loss of floral organ abscission, and asymmetric flowers subtended by a bract. Ref.1 Ref.6 Ref.9 Ref.10

Sequence similarities

Contains 3 ANK repeats.

Contains 1 BTB (POZ) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 491491Regulatory protein NPR5
PRO_0000407994

Regions

Domain26 – 11691BTB
Repeat254 – 28330ANK 1
Repeat284 – 31330ANK 2
Repeat318 – 34730ANK 3
Compositional bias403 – 43533Asn-rich
Compositional bias472 – 49019His-rich

Experimental info

Sequence conflict3861C → R in AAO22795. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q9ZVC2 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: F2E8A7133B591776

FASTA49154,001
        10         20         30         40         50         60 
MSNLEESLRS LSLDFLNLLI NGQAFSDVTF SVEGRLVHAH RCILAARSLF FRKFFCGTDS 

        70         80         90        100        110        120 
PQPVTGIDPT QHGSVPASPT RGSTAPAGII PVNSVGYEVF LLLLQFLYSG QVSIVPQKHE 

       130        140        150        160        170        180 
PRPNCGERGC WHTHCSAAVD LALDTLAASR YFGVEQLALL TQKQLASMVE KASIEDVMKV 

       190        200        210        220        230        240 
LIASRKQDMH QLWTTCSHLV AKSGLPPEIL AKHLPIDVVT KIEELRLKSS IARRSLMPHN 

       250        260        270        280        290        300 
HHHDLSVAQD LEDQKIRRMR RALDSSDVEL VKLMVMGEGL NLDESLALHY AVESCSREVV 

       310        320        330        340        350        360 
KALLELGAAD VNYPAGPAGK TPLHIAAEMV SPDMVAVLLD HHADPNVRTV GGITPLDILR 

       370        380        390        400        410        420 
TLTSDFLFKG AVPGLTHIEP NKLRLCLELV QSAAMVISRE EGNNSNNQNN DNNTGIYPHM 

       430        440        450        460        470        480 
NEEHNSGSSG GSNNNLDSRL VYLNLGAGTG QMGPGRDQGD DHNSQREGMS RHHHHHQDPS 

       490 
TMYHHHHQHH F 

« Hide

References

« Hide 'large scale' references
[1]"BLADE-ON-PETIOLE-dependent signaling controls leaf and floral patterning in Arabidopsis."
Hepworth S.R., Zhang Y., McKim S., Li X., Haughn G.W.
Plant Cell 17:1434-1448(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH PAN.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Arabidopsis ORF clones."
Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.
Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"The BLADE ON PETIOLE genes act redundantly to control the growth and development of lateral organs."
Norberg M., Holmlund M., Nilsson O.
Development 132:2203-2213(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.
[7]"Cullins 3a and 3b assemble with members of the broad complex/tramtrack/bric-a-brac (BTB) protein family to form essential ubiquitin-protein ligases (E3s) in Arabidopsis."
Gingerich D.J., Gagne J.M., Salter D.W., Hellmann H., Estelle M., Ma L., Vierstra R.D.
J. Biol. Chem. 280:18810-18821(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: DOMAIN BTB.
[8]"An Arabidopsis NPR1-like gene, NPR4, is required for disease resistance."
Liu G., Holub E.B., Alonso J.M., Ecker J.R., Fobert P.R.
Plant J. 41:304-318(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[9]"BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes."
Ha C.M., Jun J.H., Nam H.G., Fletcher J.C.
Plant Cell 19:1809-1825(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[10]"The BLADE-ON-PETIOLE genes are essential for abscission zone formation in Arabidopsis."
McKim S.M., Stenvik G.E., Butenko M.A., Kristiansen W., Cho S.K., Hepworth S.R., Aalen R.B., Haughn G.W.
Development 135:1537-1546(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[11]"Control of Arabidopsis leaf morphogenesis through regulation of the YABBY and KNOX families of transcription factors."
Ha C.M., Jun J.H., Fletcher J.C.
Genetics 186:197-206(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"BLADE-ON-PETIOLE1 coordinates organ determinacy and axial polarity in arabidopsis by directly activating ASYMMETRIC LEAVES2."
Jun J.H., Ha C.M., Fletcher J.C.
Plant Cell 22:62-76(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT.
[13]"Arabidopsis BLADE-ON-PETIOLE1 and 2 promote floral meristem fate and determinacy in a previously undefined pathway targeting APETALA1 and AGAMOUS-LIKE24."
Xu M., Hu T., McKim S.M., Murmu J., Haughn G.W., Hepworth S.R.
Plant J. 63:974-989(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY928830 mRNA. Translation: AAX22759.1.
AC005662 Genomic DNA. Translation: AAC78536.1.
CP002685 Genomic DNA. Translation: AEC09966.1.
BT002986 mRNA. Translation: AAO22795.1.
BT015773 mRNA. Translation: AAU90063.1.
PIRH84840.
RefSeqNP_181668.1. NM_129700.2.
UniGeneAt.43967.

3D structure databases

ProteinModelPortalQ9ZVC2.
SMRQ9ZVC2. Positions 203-423.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid4072. 5 interactions.
IntActQ9ZVC2. 4 interactions.
STRING3702.AT2G41370.1-P.

Proteomic databases

PaxDbQ9ZVC2.
PRIDEQ9ZVC2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G41370.1; AT2G41370.1; AT2G41370.
GeneID818735.
KEGGath:AT2G41370.

Organism-specific databases

TAIRAT2G41370.

Phylogenomic databases

eggNOGNOG298720.
HOGENOMHOG000234377.
InParanoidQ9ZVC2.
KOK14508.
OMAHYAVESC.
PhylomeDBQ9ZVC2.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

GenevestigatorQ9ZVC2.

Family and domain databases

Gene3D1.25.40.20. 1 hit.
3.30.710.10. 1 hit.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR024228. DUF3420.
[Graphical view]
PfamPF00023. Ank. 2 hits.
PF00651. BTB. 1 hit.
PF11900. DUF3420. 1 hit.
[Graphical view]
SMARTSM00248. ANK. 2 hits.
SM00225. BTB. 1 hit.
[Graphical view]
SUPFAMSSF48403. SSF48403. 1 hit.
SSF54695. SSF54695. 2 hits.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50097. BTB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNPR5_ARATH
AccessionPrimary (citable) accession number: Q9ZVC2
Secondary accession number(s): Q84WN1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: May 1, 1999
Last modified: June 11, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names