Q9ZV48 (TPS11_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 11
EC=2.4.1.15

Alternative name(s):
Trehalose-6-phosphate synthase 11
Short name=AtTPS11

Gene names

Name:	TPS11
Synonyms:	TPSB
Ordered Locus Names:	At2g18700
ORF Names:	MSF3.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	862 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.
Tissue specificity	Expressed in leaves, roots, stems and flowers. Ref.1
Sequence similarities	In the N-terminal section; belongs to the glycosyltransferase 20 family. In the C-terminal section; belongs to the trehalose phosphatase family.
Sequence caution	The sequence AAK68805.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytosol Inferred from direct assay PubMed 21166475. Source: TAIR mitochondrion Inferred from direct assay PubMed 14671022. Source: TAIR
Molecular_function	transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9ZV48-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Derived from EST data. No experimental confirmation available.

Isoform 2 (identifier: Q9ZV48-2)

The sequence of this isoform differs from the canonical sequence as follows:
     654-654: T → TRYFAKTLPFHHLPSHFCKVNTFLLLICNH

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 862	862	Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 11		PRO_0000324832
Regions
	Region	50 – 538	489	Glycosyltransferase
Natural variations
	Alternative sequence	654	1	T → TRYFAKTLPFHHLPSHFCKV NTFLLLICNH in isoform 2.		VSP_032378

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 2D2808FBFA1D5B6A	FASTA	862	98,275
	10 20 30 40 50 60 MSPESWKDQL SLVSADDYRI MGRNRIPNAV TKLSGLETDD PNGGAWVTKP KRIVVSNQLP 70 80 90 100 110 120 LRAHRDISSN KWCFEFDNDS LYLQLKDGFP PETEVVYVGS LNADVLPSEQ EDVSQFLLEK 130 140 150 160 170 180 FQCVPTFLPS DLLNKYYHGF CKHYLWPIFH YLLPMTQAQG SLFDRSNWRA YTTVNKIFAD 190 200 210 220 230 240 KIFEVLNPDD DYVWIHDYHL MILPTFLRNR FHRIKLGIFL HSPFPSSEIY RTLPVRDEIL 250 260 270 280 290 300 KGFLNCDLVG FHTFDYARHF LSCCSRMLGL DYESKRGYIG LEYFGRTVSI KILPVGIHMG 310 320 330 340 350 360 QIESIKASEK TAEKVKRLRE RFKGNIVMLG VDDLDMFKGI SLKFWAMGQL LEQNEELRGK 370 380 390 400 410 420 VVLVQITNPA RSSGKDVQDV EKQINLIADE INSKFGRPGG YKPIVFINGP VSTLDKVAYY 430 440 450 460 470 480 AISECVVVNA VRDGMNLVPY KYTVTRQGSP ALDAALGFGE DDVRKSVIIV SEFIGCSPSL 490 500 510 520 530 540 SGAIRVNPWN IDAVTNAMSS AMTMSDKEKN LRHQKHHKYI SSHNVAYWAR SYDQDLQRAC 550 560 570 580 590 600 KDHYNKRFWG VGFGLFFKVV ALDPNFRRLC GETIVPAYRR SSSRLILLDY DGTMMDQDTL 610 620 630 640 650 660 DKRPSDDLIS LLNRLCDDPS NLVFIVSGRG KDPLSKWFDS CPNLGISAEH GYFTRWNSNS 670 680 690 700 710 720 PWETSELPAD LSWKKIAKPV MNHYMEATDG SFIEEKESAM VWHHQEADHS FGSWQAKELL 730 740 750 760 770 780 DHLESVLTNE PVVVKRGQHI VEVKPQGVSK GKVVEHLIAT MRNTKGKRPD FLLCIGDDRS 790 800 810 820 830 840 DEDMFDSIVK HQDVSSIGLE EVFACTVGQK PSKAKYYLDD TPSVIKMLEW LASASDGSKH 850 860 EQQKKQSKFT FQQPMGQCRK KA
	Isoform 2 [UniParc]. Checksum: D81D3CF20D1AC1C8	FASTA	891	101,755
	10 20 30 40 50 60 MSPESWKDQL SLVSADDYRI MGRNRIPNAV TKLSGLETDD PNGGAWVTKP KRIVVSNQLP 70 80 90 100 110 120 LRAHRDISSN KWCFEFDNDS LYLQLKDGFP PETEVVYVGS LNADVLPSEQ EDVSQFLLEK 130 140 150 160 170 180 FQCVPTFLPS DLLNKYYHGF CKHYLWPIFH YLLPMTQAQG SLFDRSNWRA YTTVNKIFAD 190 200 210 220 230 240 KIFEVLNPDD DYVWIHDYHL MILPTFLRNR FHRIKLGIFL HSPFPSSEIY RTLPVRDEIL 250 260 270 280 290 300 KGFLNCDLVG FHTFDYARHF LSCCSRMLGL DYESKRGYIG LEYFGRTVSI KILPVGIHMG 310 320 330 340 350 360 QIESIKASEK TAEKVKRLRE RFKGNIVMLG VDDLDMFKGI SLKFWAMGQL LEQNEELRGK 370 380 390 400 410 420 VVLVQITNPA RSSGKDVQDV EKQINLIADE INSKFGRPGG YKPIVFINGP VSTLDKVAYY 430 440 450 460 470 480 AISECVVVNA VRDGMNLVPY KYTVTRQGSP ALDAALGFGE DDVRKSVIIV SEFIGCSPSL 490 500 510 520 530 540 SGAIRVNPWN IDAVTNAMSS AMTMSDKEKN LRHQKHHKYI SSHNVAYWAR SYDQDLQRAC 550 560 570 580 590 600 KDHYNKRFWG VGFGLFFKVV ALDPNFRRLC GETIVPAYRR SSSRLILLDY DGTMMDQDTL 610 620 630 640 650 660 DKRPSDDLIS LLNRLCDDPS NLVFIVSGRG KDPLSKWFDS CPNLGISAEH GYFTRYFAKT 670 680 690 700 710 720 LPFHHLPSHF CKVNTFLLLI CNHRWNSNSP WETSELPADL SWKKIAKPVM NHYMEATDGS 730 740 750 760 770 780 FIEEKESAMV WHHQEADHSF GSWQAKELLD HLESVLTNEP VVVKRGQHIV EVKPQGVSKG 790 800 810 820 830 840 KVVEHLIATM RNTKGKRPDF LLCIGDDRSD EDMFDSIVKH QDVSSIGLEE VFACTVGQKP 850 860 870 880 890 SKAKYYLDDT PSVIKMLEWL ASASDGSKHE QQKKQSKFTF QQPMGQCRKK A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Trehalose metabolism in Arabidopsis: occurrence of trehalose and molecular cloning and characterization of trehalose-6-phosphate synthase homologues." Vogel G., Fiehn O., Jean-Richard-dit-Bressel L., Boller T., Wiemken A., Aeschbacher R.A., Wingler A. J. Exp. Bot. 52:1817-1826(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: cv. Landsberg erecta.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 588-778 (ISOFORM 2). Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 589-862 (ISOFORM 2). Strain: cv. Columbia.
[6]	"An unexpected plethora of trehalose biosynthesis genes in Arabidopsis thaliana." Leyman B., Van Dijck P., Thevelein J.M. Trends Plant Sci. 6:510-513(2001) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC005724 Genomic DNA. Translation: AAD08939.1. CP002685 Genomic DNA. Translation: AEC06795.1. AV822913 mRNA. No translation available. AY042865 mRNA. Translation: AAK68805.1. Different initiation. AY081537 mRNA. Translation: AAM10099.1.
IPI	IPI00538425. IPI00889354.
PIR	E84567.
RefSeq	NP_179460.1. NM_127426.2.
UniGene	At.25317.
3D structure databases
HSSP	HSSP built from PDB template 1GZ5 based on UniProtKB P31677.
ProteinModelPortal	Q9ZV48.
SMR	Q9ZV48. Positions 133-538.
ModBase	Search...
Protein family/group databases
CAZy	GT20. Glycosyltransferase Family 20.
Proteomic databases
PaxDb	Q9ZV48.
PRIDE	Q9ZV48.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G18700.1; AT2G18700.1; AT2G18700.
GeneID	816385.
KEGG	ath:AT2G18700.
Organism-specific databases
TAIR	At2g18700.
Phylogenomic databases
eggNOG	COG0380.
HOGENOM	HOG000191476.
InParanoid	Q9ZV48.
KO	K16055.
OMA	DYDGTMM.
PhylomeDB	Q9ZV48.
ProtClustDB	CLSN2912887.
Gene expression databases
Genevestigator	Q9ZV48.
Family and domain databases
Gene3D	3.40.50.1000. 2 hits.
InterPro	IPR001830. Glyco_trans_20. IPR023214. HAD-like_dom. IPR006379. HAD-SF_hydro_IIB. IPR003337. Trehalose_PPase. [Graphical view]
Pfam	PF00982. Glyco_transf_20. 1 hit. PF02358. Trehalose_PPase. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01484. HAD-SF-IIB. 1 hit. TIGR00685. T6PP. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPS11_ARATH

Accession

Primary (citable) accession number: Q9ZV48
Secondary accession number(s): Q94B44

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program