Q9ZV25 (P2C23_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable protein phosphatase 2C 23
Short name=AtPP2C23
EC=3.1.3.16

Alternative name(s):
Protein POLTERGEIST-LIKE 4
Protein phosphatase 2C PLL4
Short name=PP2C PLL4

Gene names

Name:	PLL4
Ordered Locus Names:	At2g28890
ORF Names:	F8N16.18

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	654 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in leaf development regulation. Ref.4
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Subcellular location	Nucleus Probable.
Tissue specificity	Expressed in seedlings, roots, leaves, stems, young inflorescences, flowers and siliques. Ref.4
Domain	The conserved PP2C phosphatase domain (244-643) is interrupted by an insertion of approximately 100 amino acids.
Disruption phenotype	Plants show abnormal leaves altered in shape and curling. Ref.4
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	Magnesium Manganese Metal-binding
Molecular function	Developmental protein Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	leaf development Inferred from mutant phenotype Ref.4. Source: TAIR metabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay PubMed 17317660. Source: TAIR
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 654	654	Probable protein phosphatase 2C 23		PRO_0000301262
Regions
	Domain	311 – 621	311	PP2C-like
Sites
	Metal binding	280	1	Manganese 1 By similarity
	Metal binding	280	1	Manganese 2 By similarity
	Metal binding	281	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	573	1	Manganese 2 By similarity
	Metal binding	636	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZV25 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 6EEE6628C5AA060A	FASTA	654	72,482
	10 20 30 40 50 60 MGNGIGKLSK CLTGGAGRNK KPELSILEPD PLDEGLGHSF CYVRPDPTRV SSSKVHSEEE 70 80 90 100 110 120 TTTFRTISGA SVSANTATPL STSLYDPYGH IDRAAAFEST TSFSSIPLQP IPRSSGPIVP 130 140 150 160 170 180 GSGPLERGFL SGPIERGFMS GPLDGSSGPI DGKTGSDQFQ RSFSHGLANL RVGSRKGSLV 190 200 210 220 230 240 RVLRRAISKT ITRGQNSIVA PIKPVKEPDW VFGSDKTRIH QIENNLTVNS LNFSSEGSLL 250 260 270 280 290 300 DDDVSLESQN LQWAQGKAGE DRVHVVVSEE HGWLFVGIYD GFNGPDAPDY LLSHLYPAVH 310 320 330 340 350 360 RELKGLLWDD PKTDAKSSDE ADVENRDSSS EKKSKNWEES QRRWRCEWDR DLDRLLKDRS 370 380 390 400 410 420 NGLDLDPDPN SSDVLKALSQ ALRKTEEAYL ENADMMLDEN PELALMGSCV LVMLMKGEDV 430 440 450 460 470 480 YLMNVGDSRA VLGQKAESDY WIGKIKQDLE RINEETMNDF DGCGDGEGAS LVPTLSAFQL 490 500 510 520 530 540 TVDHSTNVEE EVNRIRKEHP DDASAVSNER VKGSLKVTRA FGAGFLKQPK WNNALLEMFQ 550 560 570 580 590 600 IDYKGTSPYI NCLPSLYHHR LGSKDQFLIL SSDGLYQYFT NEEAVSEVEL FITLQPEGDP 610 620 630 640 650 AQHLVQELLF RAAKKAGMDF HELLEIPQGE RRRYHDDVSI VVISLEGRMW KSCV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"POL and related phosphatases are dosage-sensitive regulators of meristem and organ development in Arabidopsis." Song S.-K., Clark S.E. Dev. Biol. 285:272-284(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE, DISRUPTION PHENOTYPE.
[5]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC005727 Genomic DNA. Translation: AAC79593.1. CP002685 Genomic DNA. Translation: AEC08185.1. AY065299 mRNA. Translation: AAL38775.1. AY096450 mRNA. Translation: AAM20090.1.
IPI	IPI00544853.
PIR	B84690.
RefSeq	NP_180455.1. NM_128448.3.
UniGene	At.27597.
3D structure databases
ProteinModelPortal	Q9ZV25.
SMR	Q9ZV25. Positions 403-645.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT2G28890.1-P.
Proteomic databases
PaxDb	Q9ZV25.
PRIDE	Q9ZV25.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G28890.1; AT2G28890.1; AT2G28890.
GeneID	817438.
KEGG	ath:AT2G28890.
Organism-specific databases
TAIR	At2g28890.
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000239375.
InParanoid	Q9ZV25.
OMA	YLENADM.
PhylomeDB	Q9ZV25.
ProtClustDB	CLSN2683691.
Gene expression databases
Genevestigator	Q9ZV25.
Family and domain databases
Gene3D	3.60.40.10. 3 hits.
InterPro	IPR001932. PP2C-like. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C23_ARATH

Accession

Primary (citable) accession number: Q9ZV25

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program