Q9ZUV3 (IRX7_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glucuronoxylan glucuronosyltransferase IRX7
EC=2.4.1.-

Alternative name(s):
Protein FRAGILE FIBER 8
Protein IRREGULAR XYLEM 7

Gene names

Name:	IRX7
Synonyms:	FRA8
Ordered Locus Names:	At2g28110
ORF Names:	F24D13.10

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the synthesis of the hemicellulose glucuronoxylan, a major component of secondary cell walls. Probably involved in the synthesis of the glycosyl sequence at the glucuronoxylan reducing end. Ref.1 Ref.5 Ref.6 Ref.7 Ref.8
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Probable Ref.1.
Tissue specificity	Expressed in developing interfascicular fibers and xylem cells in stems and developing secondary xylem in roots. Ref.1
Disruption phenotype	Dwarf phenotype. Strong reduction of secondary wall thickness, collapsed xylem vessels and reduced xylan content in cell wall. Ref.1 Ref.5 Ref.8
Sequence similarities	Belongs to the glycosyltransferase 47 family.
Sequence caution	The sequence AAL91236.1 differs from that shown. Reason: Frameshift at position 215.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW glucuronoxylan biosynthetic process Inferred from mutant phenotype Ref.8. Source: TAIR secondary cell wall biogenesis Inferred from mutant phenotype Ref.5Ref.1. Source: TAIR
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	Probable glucuronoxylan glucuronosyltransferase IRX7		PRO_0000407574
Regions
	Topological domain	1 – 16	16	Cytoplasmic Potential
	Transmembrane	17 – 37	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	38 – 448	411	Lumenal Potential
Amino acid modifications
	Glycosylation	157	1	N-linked (GlcNAc...) Potential
	Glycosylation	189	1	N-linked (GlcNAc...) Potential
	Glycosylation	287	1	N-linked (GlcNAc...) Potential
	Glycosylation	397	1	N-linked (GlcNAc...) Potential
	Glycosylation	438	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9ZUV3 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 0C71F7FDD8F0C425	FASTA	448	51,694
	10 20 30 40 50 60 MTTHKHRRTE KNLCFKQYYK WILCFILTLY FFASFFVDHD QDHRSSTSIS KHLLTNHKPK 70 80 90 100 110 120 LFASRAMFES KIHDHKLGFT SQQPNIKTDV FNNLKIYVYD LPSKFNKDWL ANDRCTNHLF 130 140 150 160 170 180 AAEVALHKAF LSLEGDVRTE DPYEADFFFV PVYVSCNFST INGFPAIGHA RSLINDAIKL 190 200 210 220 230 240 VSTQYPFWNR TSGSDHVFTA THDFGSCFHT MEDRAIADGV PIFLRNSIIL QTFGVTFNHP 250 260 270 280 290 300 CQEVENVVIP PYISPESLHK TQKNIPVTKE RDIWVFFRGK MELHPKNISG RFYSKRVRTN 310 320 330 340 350 360 IWRSYGGDRR FYLQRQRFAG YQSEIARSVF CLCPLGWAPW SPRLVESVAL GCVPVIIADG 370 380 390 400 410 420 IRLPFPSTVR WPDISLTVAE RDVGKLGDIL EHVAATNLSV IQRNLEDPSV RRALMFNVPS 430 440 REGDATWQVL EALSKKLNRS VRRSNSFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arabidopsis fragile fiber8, which encodes a putative glucuronyltransferase, is essential for normal secondary wall synthesis." Zhong R., Pena M.J., Zhou G.K., Nairn C.J., Wood-Jones A., Richardson E.A., Morrison W.H. III, Darvill A.G., York W.S., Ye Z.H. Plant Cell 17:3390-3408(2005) [PubMed: 16272433] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Identification of novel genes in Arabidopsis involved in secondary cell wall formation using expression profiling and reverse genetics." Brown D.M., Zeef L.A.H., Ellis J., Goodacre R., Turner S.R. Plant Cell 17:2281-2295(2005) [PubMed: 15980264] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[6]	"The irregular xylem9 mutant is deficient in xylan xylosyltransferase activity." Lee C., O'Neill M.A., Tsumuraya Y., Darvill A.G., Ye Z.H. Plant Cell Physiol. 48:1624-1634(2007) [PubMed: 17938130] [Abstract] Cited for: FUNCTION.
[7]	"The PARVUS gene is expressed in cells undergoing secondary wall thickening and is essential for glucuronoxylan biosynthesis." Lee C., Zhong R., Richardson E.A., Himmelsbach D.S., McPhail B.T., Ye Z.H. Plant Cell Physiol. 48:1659-1672(2007) [PubMed: 17991630] [Abstract] Cited for: FUNCTION.
[8]	"Comparison of five xylan synthesis mutants reveals new insight into the mechanisms of xylan synthesis." Brown D.M., Goubet F., Wong V.W., Goodacre R., Stephens E., Dupree P., Turner S.R. Plant J. 52:1154-1168(2007) [PubMed: 17944810] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ182567 mRNA. Translation: ABA60868.1. DQ182568 Genomic DNA. Translation: ABA60869.1. AC005851 Genomic DNA. Translation: AAC98455.1. CP002685 Genomic DNA. Translation: AEC08081.1. AY081347 mRNA. Translation: AAL91236.1. Frameshift. BT006256 mRNA. Translation: AAP13364.1.
IPI	IPI00528947.
PIR	H84680.
RefSeq	NP_850113.2. NM_179782.3.
UniGene	At.38661.
3D structure databases
ProteinModelPortal	Q9ZUV3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9ZUV3.
Protein family/group databases
CAZy	GT47. Glycosyltransferase Family 47.
Proteomic databases
PRIDE	Q9ZUV3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G28110.1; AT2G28110.1; AT2G28110.
GeneID	817357.
GenomeReviews	Gene locus AT2G28110 in contig CT485783_GR.
KEGG	ath:AT2G28110.
NMPDR	fig|3702.1.peg.9860.
Organism-specific databases
TAIR	At2g28110.
Phylogenomic databases
GeneTree	EPGT00070000030424.
InParanoid	Q9ZUV3.
OMA	VIPPYIS.
PhylomeDB	Q9ZUV3.
ProtClustDB	CLSN2681061.
Gene expression databases
Genevestigator	Q9ZUV3.
Family and domain databases
InterPro	IPR004263. Exostosin. [Graphical view]
Pfam	PF03016. Exostosin. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IRX7_ARATH

Accession

Primary (citable) accession number: Q9ZUV3
Secondary accession number(s): Q8RXC5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 3, 2011
Last sequence update:	May 1, 1999
Last modified:	December 14, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program