Q9ZUM9 (ASHR2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histone-lysine N-methyltransferase ASHR2
EC=2.1.1.43

Alternative name(s):
ASH1-related protein 2
Protein SET DOMAIN GROUP 39

Gene names

Name:	ASHR2
Synonyms:	SDG39, SET39
Ordered Locus Names:	At2g19640
ORF Names:	F3P11.24

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Histone methyltransferase By similarity.
Catalytic activity	S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].
Subcellular location	Nucleus By similarity. Chromosome By similarity.
Sequence similarities	Belongs to the histone-lysine methyltransferase family. SET2 subfamily. Contains 1 SET domain.

Ontologies

Keywords
Cellular component	Chromosome Nucleus
Coding sequence diversity	Alternative splicing
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	chromosome Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	histone-lysine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9ZUM9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9ZUM9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     330-341: GENEEEVCGNGV → DICVRRRIVLAL
     342-398: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	Histone-lysine N-methyltransferase ASHR2		PRO_0000233375
Regions
	Domain	147 – 274	128	SET
	Compositional bias	53 – 184	132	Ser-rich
	Compositional bias	299 – 335	37	Glu-rich
Natural variations
	Alternative sequence	330 – 341	12	GENEE…CGNGV → DICVRRRIVLAL in isoform 2.		VSP_018134
	Alternative sequence	342 – 398	57	Missing in isoform 2.		VSP_018135

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 2, 2006. Version 3. Checksum: 32CFC10C8B546F64	FASTA	398	43,761
	10 20 30 40 50 60 MINDGGAKPE TLLRVAEIGG RGRSLVAAQS LRAGQVILRE SPLLLYSAFP FLSSSVSPYC 70 80 90 100 110 120 DHCFRLLASS AHQKCQSCSL VSFCSPNCFA SHTPWLCESL RRLHQSSSSA FSDQPSDRQV 130 140 150 160 170 180 QARFLLSAYN LAAASPSDFQ ILLSLQGSGS SNGDPSCSAG DSAAAGFLHS LLSSVCPSLP 190 200 210 220 230 240 VSISPDLTAA LLSKDKVNAF GLMEPCSVSN EKRSVRAYGI YPKTSFFNHD CLPNACRFDY 250 260 270 280 290 300 VDSASDGNTD IIIRMIHDVP EGREVCLSYF PVNMNYSSRQ KRLLEDYGFK CDCDRCKVEF 310 320 330 340 350 360 SWSEGEEDEN EIMEEMEDQD EQEEMEDSVG ENEEEVCGNG VDDESNFPHA YFFVRYMCEK 370 380 390 ENCFGTLAPL PPKTHDASRV LECNVCGSVK EDEVGVNQ
	Isoform 2 [UniParc]. Checksum: A206767A5E2E3FD2	FASTA	341	37,532
	10 20 30 40 50 60 MINDGGAKPE TLLRVAEIGG RGRSLVAAQS LRAGQVILRE SPLLLYSAFP FLSSSVSPYC 70 80 90 100 110 120 DHCFRLLASS AHQKCQSCSL VSFCSPNCFA SHTPWLCESL RRLHQSSSSA FSDQPSDRQV 130 140 150 160 170 180 QARFLLSAYN LAAASPSDFQ ILLSLQGSGS SNGDPSCSAG DSAAAGFLHS LLSSVCPSLP 190 200 210 220 230 240 VSISPDLTAA LLSKDKVNAF GLMEPCSVSN EKRSVRAYGI YPKTSFFNHD CLPNACRFDY 250 260 270 280 290 300 VDSASDGNTD IIIRMIHDVP EGREVCLSYF PVNMNYSSRQ KRLLEDYGFK CDCDRCKVEF 310 320 330 340 SWSEGEEDEN EIMEEMEDQD EQEEMEDSVD ICVRRRIVLA L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: cv. Columbia.
[4]	"The Arabidopsis thaliana genome contains at least 29 active genes encoding SET domain proteins that can be assigned to four evolutionarily conserved classes." Baumbusch L.O., Thorstensen T., Krauss V., Fischer A., Naumann K., Assalkhou R., Schulz I., Reuter G., Aalen R.B. Nucleic Acids Res. 29:4319-4333(2001) [PubMed: 11691919] [Abstract] Cited for: NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC005917 Genomic DNA. Translation: AAD10162.2. CP002685 Genomic DNA. Translation: AEC06906.1. CP002685 Genomic DNA. Translation: AEC06907.1. AY034953 mRNA. Translation: AAK59459.1. AY070027 mRNA. Translation: AAL47498.1. BT004010 mRNA. Translation: AAO42047.1.
IPI	IPI00516577. IPI00545100.
PIR	C84579.
RefSeq	NP_565457.1. NM_127522.2. NP_849991.1. NM_179660.1.
UniGene	At.23821.
3D structure databases
ProteinModelPortal	Q9ZUM9.
SMR	Q9ZUM9. Positions 223-271.
ModBase	Search...
Proteomic databases
PRIDE	Q9ZUM9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G19640.2; AT2G19640.2; AT2G19640.
GeneID	816483.
GenomeReviews	Gene locus AT2G19640 in contig CT485783_GR.
KEGG	ath:AT2G19640.
NMPDR	fig|3702.1.peg.8958.
Organism-specific databases
TAIR	At2g19640.
Phylogenomic databases
eggNOG	KOG2084.
GeneTree	EPGT00070000030233.
HOGENOM	HBG601688.
InParanoid	Q9ZUM9.
OMA	DCLPNAC.
PhylomeDB	Q9ZUM9.
ProtClustDB	CLSN2688255.
Gene expression databases
Genevestigator	Q9ZUM9.
GermOnline	AT2G19640. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001214. SET_dom. [Graphical view]
Pfam	PF00856. SET. 1 hit. [Graphical view]
SMART	SM00317. SET. 1 hit. [Graphical view]
PROSITE	PS50280. SET. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASHR2_ARATH

Accession

Primary (citable) accession number: Q9ZUM9
Secondary accession number(s): Q84WB9, Q94CD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	May 2, 2006
Last modified:	September 21, 2011
This is version 66 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program