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Q9ZUA5

- VIT1_ARATH

UniProt

Q9ZUA5 - VIT1_ARATH

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Protein

Vacuolar iron transporter 1

Gene

VIT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Vacuolar iron transporter involved in the transfer of iron from the cytosol to the vacuole for intracellular iron storage. Vacuolar iron storage is required for seed embryo and seedling development.1 Publication

GO - Molecular functioni

  1. iron ion transmembrane transporter activity Source: TAIR

GO - Biological processi

  1. intracellular sequestering of iron ion Source: TAIR
  2. iron ion homeostasis Source: TAIR
  3. iron ion transmembrane transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

Iron

Protein family/group databases

TCDBi2.A.89.1.2. the vacuolar iron transporter (vit) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar iron transporter 1
Short name:
AtVIT1
Gene namesi
Name:VIT1
Ordered Locus Names:At2g01770
ORF Names:T8O11.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G01770.

Subcellular locationi

Vacuole membrane 1 Publication; Multi-pass membrane protein 1 Publication

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth condition, but important reduced growth and leaves with severe chlorosis when grown on soil at pH 7.9 that causes limited iron availability.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 250250Vacuolar iron transporter 1PRO_0000411004Add
BLAST

Expressioni

Tissue specificityi

Highly expressed in developing embryo and seed. Expressed in young seedlings, predominantly in the vasculature.1 Publication

Gene expression databases

GenevestigatoriQ9ZUA5.

Interactioni

Protein-protein interaction databases

BioGridi111. 4 interactions.
IntActiQ9ZUA5. 4 interactions.
STRINGi3702.AT2G01770.1-P.

Structurei

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3737CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini59 – 646VacuolarSequence Analysis
Topological domaini86 – 16984CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini191 – 1922VacuolarSequence Analysis
Topological domaini214 – 22714CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini249 – 2502VacuolarSequence Analysis

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei38 – 5821HelicalSequence AnalysisAdd
BLAST
Transmembranei65 – 8521HelicalSequence AnalysisAdd
BLAST
Transmembranei170 – 19021HelicalSequence AnalysisAdd
BLAST
Transmembranei193 – 21321HelicalSequence AnalysisAdd
BLAST
Transmembranei228 – 24821HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the CCC1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1814.
HOGENOMiHOG000096548.
InParanoidiQ9ZUA5.
OMAiLDFMMRF.
PhylomeDBiQ9ZUA5.

Family and domain databases

InterProiIPR008217. DUF125_TM.
[Graphical view]
PfamiPF01988. VIT1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZUA5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSEEDKITR ISIEPEKQTL LDHHTEKHFT AGEIVRDIII GVSDGLTVPF
60 70 80 90 100
ALAAGLSGAN ASSSIVLTAG IAEVAAGAIS MGLGGYLAAK SEEDHYAREM
110 120 130 140 150
KREQEEIVAV PETEAAEVAE ILAQYGIEPH EYSPVVNALR KNPQAWLDFM
160 170 180 190 200
MRFELGLEKP DPKRALQSAF TIAIAYVLGG FIPLLPYMLI PHAMDAVVAS
210 220 230 240 250
VVITLFALFI FGYAKGHFTG SKPLRSAFET AFIGAIASAA AFCLAKVVQH
Length:250
Mass (Da):26,860
Last modified:May 1, 1999 - v1
Checksum:i4B481BE5476DF348
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY316134 mRNA. Translation: AAQ87602.1.
AC006069 Genomic DNA. Translation: AAD12695.1.
CP002685 Genomic DNA. Translation: AEC05496.1.
BT028987 mRNA. Translation: ABI93896.1.
PIRiH84428.
RefSeqiNP_178286.1. NM_126238.2.
UniGeneiAt.52589.

Genome annotation databases

EnsemblPlantsiAT2G01770.1; AT2G01770.1; AT2G01770.
GeneIDi814708.
KEGGiath:AT2G01770.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY316134 mRNA. Translation: AAQ87602.1 .
AC006069 Genomic DNA. Translation: AAD12695.1 .
CP002685 Genomic DNA. Translation: AEC05496.1 .
BT028987 mRNA. Translation: ABI93896.1 .
PIRi H84428.
RefSeqi NP_178286.1. NM_126238.2.
UniGenei At.52589.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 111. 4 interactions.
IntActi Q9ZUA5. 4 interactions.
STRINGi 3702.AT2G01770.1-P.

Protein family/group databases

TCDBi 2.A.89.1.2. the vacuolar iron transporter (vit) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G01770.1 ; AT2G01770.1 ; AT2G01770 .
GeneIDi 814708.
KEGGi ath:AT2G01770.

Organism-specific databases

TAIRi AT2G01770.

Phylogenomic databases

eggNOGi COG1814.
HOGENOMi HOG000096548.
InParanoidi Q9ZUA5.
OMAi LDFMMRF.
PhylomeDBi Q9ZUA5.

Gene expression databases

Genevestigatori Q9ZUA5.

Family and domain databases

InterProi IPR008217. DUF125_TM.
[Graphical view ]
Pfami PF01988. VIT1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Localization of iron in Arabidopsis seed requires the vacuolar membrane transporter VIT1."
    Kim S.A., Punshon T., Lanzirotti A., Li L., Alonso J.M., Ecker J.R., Kaplan J., Guerinot M.L.
    Science 314:1295-1298(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.
    Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiVIT1_ARATH
AccessioniPrimary (citable) accession number: Q9ZUA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: May 1, 1999
Last modified: October 1, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Can mediate iron sequestration into vacuoles when expressed in the yeast ccc1 mutant. In seeds, iron is strongly localized to the provascular strands of the hypocotyl, radicle, and cotyledons, but completely absent from these cells in vit1-1 mutant.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3