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Q9ZUA5

- VIT1_ARATH

UniProt

Q9ZUA5 - VIT1_ARATH

Protein

Vacuolar iron transporter 1

Gene

VIT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Vacuolar iron transporter involved in the transfer of iron from the cytosol to the vacuole for intracellular iron storage. Vacuolar iron storage is required for seed embryo and seedling development.1 Publication

    GO - Molecular functioni

    1. iron ion transmembrane transporter activity Source: TAIR

    GO - Biological processi

    1. intracellular sequestering of iron ion Source: TAIR
    2. iron ion homeostasis Source: TAIR
    3. iron ion transmembrane transport Source: GOC

    Keywords - Biological processi

    Ion transport, Iron transport, Transport

    Keywords - Ligandi

    Iron

    Protein family/group databases

    TCDBi2.A.89.1.2. the vacuolar iron transporter (vit) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vacuolar iron transporter 1
    Short name:
    AtVIT1
    Gene namesi
    Name:VIT1
    Ordered Locus Names:At2g01770
    ORF Names:T8O11.6
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G01770.

    Subcellular locationi

    Vacuole membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. vacuolar membrane Source: TAIR

    Keywords - Cellular componenti

    Membrane, Vacuole

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype under normal growth condition, but important reduced growth and leaves with severe chlorosis when grown on soil at pH 7.9 that causes limited iron availability.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 250250Vacuolar iron transporter 1PRO_0000411004Add
    BLAST

    Expressioni

    Tissue specificityi

    Highly expressed in developing embryo and seed. Expressed in young seedlings, predominantly in the vasculature.1 Publication

    Gene expression databases

    GenevestigatoriQ9ZUA5.

    Interactioni

    Protein-protein interaction databases

    BioGridi111. 4 interactions.
    IntActiQ9ZUA5. 4 interactions.
    STRINGi3702.AT2G01770.1-P.

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3737CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini59 – 646VacuolarSequence Analysis
    Topological domaini86 – 16984CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini191 – 1922VacuolarSequence Analysis
    Topological domaini214 – 22714CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini249 – 2502VacuolarSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei38 – 5821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei65 – 8521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei170 – 19021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei193 – 21321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei228 – 24821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the CCC1 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1814.
    HOGENOMiHOG000096548.
    InParanoidiQ9ZUA5.
    OMAiLDFMMRF.
    PhylomeDBiQ9ZUA5.

    Family and domain databases

    InterProiIPR008217. DUF125_TM.
    [Graphical view]
    PfamiPF01988. VIT1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9ZUA5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSEEDKITR ISIEPEKQTL LDHHTEKHFT AGEIVRDIII GVSDGLTVPF    50
    ALAAGLSGAN ASSSIVLTAG IAEVAAGAIS MGLGGYLAAK SEEDHYAREM 100
    KREQEEIVAV PETEAAEVAE ILAQYGIEPH EYSPVVNALR KNPQAWLDFM 150
    MRFELGLEKP DPKRALQSAF TIAIAYVLGG FIPLLPYMLI PHAMDAVVAS 200
    VVITLFALFI FGYAKGHFTG SKPLRSAFET AFIGAIASAA AFCLAKVVQH 250
    Length:250
    Mass (Da):26,860
    Last modified:May 1, 1999 - v1
    Checksum:i4B481BE5476DF348
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY316134 mRNA. Translation: AAQ87602.1.
    AC006069 Genomic DNA. Translation: AAD12695.1.
    CP002685 Genomic DNA. Translation: AEC05496.1.
    BT028987 mRNA. Translation: ABI93896.1.
    PIRiH84428.
    RefSeqiNP_178286.1. NM_126238.2.
    UniGeneiAt.52589.

    Genome annotation databases

    EnsemblPlantsiAT2G01770.1; AT2G01770.1; AT2G01770.
    GeneIDi814708.
    KEGGiath:AT2G01770.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY316134 mRNA. Translation: AAQ87602.1 .
    AC006069 Genomic DNA. Translation: AAD12695.1 .
    CP002685 Genomic DNA. Translation: AEC05496.1 .
    BT028987 mRNA. Translation: ABI93896.1 .
    PIRi H84428.
    RefSeqi NP_178286.1. NM_126238.2.
    UniGenei At.52589.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111. 4 interactions.
    IntActi Q9ZUA5. 4 interactions.
    STRINGi 3702.AT2G01770.1-P.

    Protein family/group databases

    TCDBi 2.A.89.1.2. the vacuolar iron transporter (vit) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G01770.1 ; AT2G01770.1 ; AT2G01770 .
    GeneIDi 814708.
    KEGGi ath:AT2G01770.

    Organism-specific databases

    TAIRi AT2G01770.

    Phylogenomic databases

    eggNOGi COG1814.
    HOGENOMi HOG000096548.
    InParanoidi Q9ZUA5.
    OMAi LDFMMRF.
    PhylomeDBi Q9ZUA5.

    Gene expression databases

    Genevestigatori Q9ZUA5.

    Family and domain databases

    InterProi IPR008217. DUF125_TM.
    [Graphical view ]
    Pfami PF01988. VIT1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Localization of iron in Arabidopsis seed requires the vacuolar membrane transporter VIT1."
      Kim S.A., Punshon T., Lanzirotti A., Li L., Alonso J.M., Ecker J.R., Kaplan J., Guerinot M.L.
      Science 314:1295-1298(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Arabidopsis ORF clones."
      Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.
      Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiVIT1_ARATH
    AccessioniPrimary (citable) accession number: Q9ZUA5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 27, 2011
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Can mediate iron sequestration into vacuoles when expressed in the yeast ccc1 mutant. In seeds, iron is strongly localized to the provascular strands of the hypocotyl, radicle, and cotyledons, but completely absent from these cells in vit1-1 mutant.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3