Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9ZUA5 (VIT1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vacuolar iron transporter 1

Short name=AtVIT1
Gene names
Name:VIT1
Ordered Locus Names:At2g01770
ORF Names:T8O11.6
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length250 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Vacuolar iron transporter involved in the transfer of iron from the cytosol to the vacuole for intracellular iron storage. Vacuolar iron storage is required for seed embryo and seedling development. Ref.1

Subcellular location

Vacuole membrane; Multi-pass membrane protein Ref.1.

Tissue specificity

Highly expressed in developing embryo and seed. Expressed in young seedlings, predominantly in the vasculature. Ref.1

Disruption phenotype

No visible phenotype under normal growth condition, but important reduced growth and leaves with severe chlorosis when grown on soil at pH 7.9 that causes limited iron availability. Ref.1

Miscellaneous

Can mediate iron sequestration into vacuoles when expressed in the yeast ccc1 mutant. In seeds, iron is strongly localized to the provascular strands of the hypocotyl, radicle, and cotyledons, but completely absent from these cells in vit1-1 mutant.

Sequence similarities

Belongs to the CCC1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 250250Vacuolar iron transporter 1
PRO_0000411004

Regions

Topological domain1 – 3737Cytoplasmic Potential
Transmembrane38 – 5821Helical; Potential
Topological domain59 – 646Vacuolar Potential
Transmembrane65 – 8521Helical; Potential
Topological domain86 – 16984Cytoplasmic Potential
Transmembrane170 – 19021Helical; Potential
Topological domain191 – 1922Vacuolar Potential
Transmembrane193 – 21321Helical; Potential
Topological domain214 – 22714Cytoplasmic Potential
Transmembrane228 – 24821Helical; Potential
Topological domain249 – 2502Vacuolar Potential

Sequences

Sequence LengthMass (Da)Tools
Q9ZUA5 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 4B481BE5476DF348

FASTA25026,860
        10         20         30         40         50         60 
MSSEEDKITR ISIEPEKQTL LDHHTEKHFT AGEIVRDIII GVSDGLTVPF ALAAGLSGAN 

        70         80         90        100        110        120 
ASSSIVLTAG IAEVAAGAIS MGLGGYLAAK SEEDHYAREM KREQEEIVAV PETEAAEVAE 

       130        140        150        160        170        180 
ILAQYGIEPH EYSPVVNALR KNPQAWLDFM MRFELGLEKP DPKRALQSAF TIAIAYVLGG 

       190        200        210        220        230        240 
FIPLLPYMLI PHAMDAVVAS VVITLFALFI FGYAKGHFTG SKPLRSAFET AFIGAIASAA 

       250 
AFCLAKVVQH 

« Hide

References

« Hide 'large scale' references
[1]"Localization of iron in Arabidopsis seed requires the vacuolar membrane transporter VIT1."
Kim S.A., Punshon T., Lanzirotti A., Li L., Alonso J.M., Ecker J.R., Kaplan J., Guerinot M.L.
Science 314:1295-1298(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Arabidopsis ORF clones."
Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.
Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY316134 mRNA. Translation: AAQ87602.1.
AC006069 Genomic DNA. Translation: AAD12695.1.
CP002685 Genomic DNA. Translation: AEC05496.1.
BT028987 mRNA. Translation: ABI93896.1.
PIRH84428.
RefSeqNP_178286.1. NM_126238.2.
UniGeneAt.52589.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111. 4 interactions.
IntActQ9ZUA5. 4 interactions.
STRING3702.AT2G01770.1-P.

Protein family/group databases

TCDB2.A.89.1.2. the vacuolar iron transporter (vit) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G01770.1; AT2G01770.1; AT2G01770.
GeneID814708.
KEGGath:AT2G01770.

Organism-specific databases

TAIRAT2G01770.

Phylogenomic databases

eggNOGCOG1814.
HOGENOMHOG000096548.
InParanoidQ9ZUA5.
OMAEIMFELG.
PhylomeDBQ9ZUA5.
ProtClustDBCLSN2683546.

Gene expression databases

GenevestigatorQ9ZUA5.

Family and domain databases

InterProIPR008217. DUF125_TM.
[Graphical view]
PfamPF01988. VIT1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameVIT1_ARATH
AccessionPrimary (citable) accession number: Q9ZUA5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: May 1, 1999
Last modified: February 19, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names