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Protein

Glucan endo-1,3-beta-glucosidase 3

Gene

At2g01630

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei263 – 2631NucleophileBy similarity
Active sitei326 – 3261Proton donorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Plant defense

Enzyme and pathway databases

BioCyciARA:AT2G01630-MONOMER.
ARA:GQT-2626-MONOMER.

Protein family/group databases

CAZyiCBM43. Carbohydrate-Binding Module Family 43.
GH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase 3 (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase 3
Short name:
(1->3)-beta-glucanase 3
Beta-1,3-endoglucanase 3
Short name:
Beta-1,3-glucanase 3
Gene namesi
Ordered Locus Names:At2g01630
ORF Names:T8O11.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G01630.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: TAIR
  • anchored component of plasma membrane Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Chaini19 – 483465Glucan endo-1,3-beta-glucosidase 3PRO_0000011886Add
BLAST
Propeptidei484 – 50118Removed in mature formSequence AnalysisPRO_0000011887Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi88 – 881N-linked (GlcNAc...)Sequence Analysis
Glycosylationi107 – 1071N-linked (GlcNAc...)Sequence Analysis
Glycosylationi171 – 1711N-linked (GlcNAc...)Sequence Analysis
Glycosylationi253 – 2531N-linked (GlcNAc...)Sequence Analysis
Glycosylationi295 – 2951N-linked (GlcNAc...)Sequence Analysis
Glycosylationi353 – 3531N-linked (GlcNAc...)Sequence Analysis
Glycosylationi357 – 3571N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi361 ↔ 424By similarity
Glycosylationi451 – 4511N-linked (GlcNAc...)Sequence Analysis
Glycosylationi456 – 4561N-linked (GlcNAc...)Sequence Analysis
Glycosylationi457 – 4571N-linked (GlcNAc...)Sequence Analysis
Glycosylationi466 – 4661N-linked (GlcNAc...)Sequence Analysis
Lipidationi483 – 4831GPI-anchor amidated serineSequence Analysis

Post-translational modificationi

Contains two additional disulfide bonds.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ9ZU91.
PRIDEiQ9ZU91.

Expressioni

Gene expression databases

ExpressionAtlasiQ9ZU91. baseline and differential.
GenevestigatoriQ9ZU91.

Structurei

3D structure databases

ProteinModelPortaliQ9ZU91.
SMRiQ9ZU91. Positions 23-342, 359-448.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG290245.
HOGENOMiHOG000238220.
InParanoidiQ9ZU91.
OMAiAAYFSMS.
PhylomeDBiQ9ZU91.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
PF07983. X8. 1 hit.
[Graphical view]
SMARTiSM00768. X8. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9ZU91-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAALLLLFLF LFASSALSQD SLIGVNIGTE VTNMPSPTQV VALLKSQNIN
60 70 80 90 100
RVRLYDADRS MLLAFAHTGV QVIISVPNDQ LLGISQSNAT AANWVTRNVA
110 120 130 140 150
AYYPATNITT IAVGSEVLTS LTNAASVLVS ALKYIQAALV TANLDRQIKV
160 170 180 190 200
STPHSSTIIL DSFPPSQAFF NKTWDPVIVP LLKFLQSTGS PLLLNVYPYF
210 220 230 240 250
DYVQSNGVIP LDYALFQPLQ ANKEAVDANT LLHYTNVFDA IVDAAYFAMS
260 270 280 290 300
YLNFTNIPIV VTESGWPSKG GPSEHDATVE NANTYNSNLI QHVINKTGTP
310 320 330 340 350
KHPGTAVTTY IYELYNEDTR PGPVSEKNWG LFYTNGTPVY TLRLAGAGAI
360 370 380 390 400
LANDTTNQTF CIAKEKVDRK MLQAALDWAC GPGKVDCSAL MQGESCYEPD
410 420 430 440 450
DVVAHSTYAF NAYYQKMGKA SGSCDFKGVA TVTTTDPSRG TCVFPGSAKS
460 470 480 490 500
NQTLGNNTSA LAPSANSTTS GCIPKYYHHP HASFGDLTLL SLLLIIALVF

L
Length:501
Mass (Da):53,992
Last modified:June 1, 2002 - v2
Checksum:i3858B1C25FD37920
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006069 Genomic DNA. Translation: AAD12708.2.
CP002685 Genomic DNA. Translation: AEC05475.1.
AY085500 mRNA. Translation: AAM62724.1.
PIRiB84427.
RefSeqiNP_565269.1. NM_126224.2. [Q9ZU91-1]
UniGeneiAt.27628.
At.42475.
At.75580.

Genome annotation databases

EnsemblPlantsiAT2G01630.1; AT2G01630.1; AT2G01630. [Q9ZU91-1]
GeneIDi814692.
KEGGiath:AT2G01630.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006069 Genomic DNA. Translation: AAD12708.2.
CP002685 Genomic DNA. Translation: AEC05475.1.
AY085500 mRNA. Translation: AAM62724.1.
PIRiB84427.
RefSeqiNP_565269.1. NM_126224.2. [Q9ZU91-1]
UniGeneiAt.27628.
At.42475.
At.75580.

3D structure databases

ProteinModelPortaliQ9ZU91.
SMRiQ9ZU91. Positions 23-342, 359-448.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM43. Carbohydrate-Binding Module Family 43.
GH17. Glycoside Hydrolase Family 17.

Proteomic databases

PaxDbiQ9ZU91.
PRIDEiQ9ZU91.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G01630.1; AT2G01630.1; AT2G01630. [Q9ZU91-1]
GeneIDi814692.
KEGGiath:AT2G01630.

Organism-specific databases

TAIRiAT2G01630.

Phylogenomic databases

eggNOGiNOG290245.
HOGENOMiHOG000238220.
InParanoidiQ9ZU91.
OMAiAAYFSMS.
PhylomeDBiQ9ZU91.

Enzyme and pathway databases

BioCyciARA:AT2G01630-MONOMER.
ARA:GQT-2626-MONOMER.

Miscellaneous databases

PROiQ9ZU91.

Gene expression databases

ExpressionAtlasiQ9ZU91. baseline and differential.
GenevestigatoriQ9ZU91.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
PF07983. X8. 1 hit.
[Graphical view]
SMARTiSM00768. X8. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Identification of glycosylphosphatidylinositol-anchored proteins in Arabidopsis. A proteomic and genomic analysis."
    Borner G.H.H., Lilley K.S., Stevens T.J., Dupree P.
    Plant Physiol. 132:568-577(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.
    Tissue: Callus.
  5. "Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins."
    Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C., Jensen O.N.
    Mol. Cell. Proteomics 2:1261-1270(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].
  6. "Modification-specific proteomics of plasma membrane proteins: identification and characterization of glycosylphosphatidylinositol-anchored proteins released upon phospholipase D treatment."
    Elortza F., Mohammed S., Bunkenborg J., Foster L.J., Nuehse T.S., Brodbeck U., Peck S.C., Jensen O.N.
    J. Proteome Res. 5:935-943(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiE133_ARATH
AccessioniPrimary (citable) accession number: Q9ZU91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: June 1, 2002
Last modified: April 29, 2015
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.