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Protein

Glucan endo-1,3-beta-glucosidase 3

Gene

At2g01630

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei263NucleophileBy similarity1
Active sitei326Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Plant defense

Enzyme and pathway databases

BioCyciARA:AT2G01630-MONOMER.

Protein family/group databases

CAZyiCBM43. Carbohydrate-Binding Module Family 43.
GH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase 3 (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase 3
Short name:
(1->3)-beta-glucanase 3
Beta-1,3-endoglucanase 3
Short name:
Beta-1,3-glucanase 3
Gene namesi
Ordered Locus Names:At2g01630
ORF Names:T8O11.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G01630.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: TAIR
  • anchored component of plasma membrane Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000001188619 – 483Glucan endo-1,3-beta-glucosidase 3Add BLAST465
PropeptideiPRO_0000011887484 – 501Removed in mature formSequence analysisAdd BLAST18

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi88N-linked (GlcNAc...)Sequence analysis1
Glycosylationi107N-linked (GlcNAc...)Sequence analysis1
Glycosylationi171N-linked (GlcNAc...)Sequence analysis1
Glycosylationi253N-linked (GlcNAc...)Sequence analysis1
Glycosylationi295N-linked (GlcNAc...)Sequence analysis1
Glycosylationi353N-linked (GlcNAc...)Sequence analysis1
Glycosylationi357N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi361 ↔ 424By similarity
Glycosylationi451N-linked (GlcNAc...)Sequence analysis1
Glycosylationi456N-linked (GlcNAc...)Sequence analysis1
Glycosylationi457N-linked (GlcNAc...)Sequence analysis1
Glycosylationi466N-linked (GlcNAc...)Sequence analysis1
Lipidationi483GPI-anchor amidated serineSequence analysis1

Post-translational modificationi

Contains two additional disulfide bonds.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ9ZU91.

Expressioni

Gene expression databases

ExpressionAtlasiQ9ZU91. baseline and differential.
GenevisibleiQ9ZU91. AT.

Structurei

3D structure databases

ProteinModelPortaliQ9ZU91.
SMRiQ9ZU91.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IEQ4. Eukaryota.
ENOG410ZF4V. LUCA.
HOGENOMiHOG000238220.
InParanoidiQ9ZU91.
KOiK19891.
OMAiPLQANKE.
OrthoDBiEOG093607VJ.
PhylomeDBiQ9ZU91.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
PF07983. X8. 1 hit.
[Graphical view]
SMARTiSM00768. X8. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9ZU91-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAALLLLFLF LFASSALSQD SLIGVNIGTE VTNMPSPTQV VALLKSQNIN
60 70 80 90 100
RVRLYDADRS MLLAFAHTGV QVIISVPNDQ LLGISQSNAT AANWVTRNVA
110 120 130 140 150
AYYPATNITT IAVGSEVLTS LTNAASVLVS ALKYIQAALV TANLDRQIKV
160 170 180 190 200
STPHSSTIIL DSFPPSQAFF NKTWDPVIVP LLKFLQSTGS PLLLNVYPYF
210 220 230 240 250
DYVQSNGVIP LDYALFQPLQ ANKEAVDANT LLHYTNVFDA IVDAAYFAMS
260 270 280 290 300
YLNFTNIPIV VTESGWPSKG GPSEHDATVE NANTYNSNLI QHVINKTGTP
310 320 330 340 350
KHPGTAVTTY IYELYNEDTR PGPVSEKNWG LFYTNGTPVY TLRLAGAGAI
360 370 380 390 400
LANDTTNQTF CIAKEKVDRK MLQAALDWAC GPGKVDCSAL MQGESCYEPD
410 420 430 440 450
DVVAHSTYAF NAYYQKMGKA SGSCDFKGVA TVTTTDPSRG TCVFPGSAKS
460 470 480 490 500
NQTLGNNTSA LAPSANSTTS GCIPKYYHHP HASFGDLTLL SLLLIIALVF

L
Length:501
Mass (Da):53,992
Last modified:June 1, 2002 - v2
Checksum:i3858B1C25FD37920
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006069 Genomic DNA. Translation: AAD12708.2.
CP002685 Genomic DNA. Translation: AEC05475.1.
AY085500 mRNA. Translation: AAM62724.1.
PIRiB84427.
RefSeqiNP_565269.1. NM_126224.2. [Q9ZU91-1]
UniGeneiAt.27628.
At.42475.
At.75580.

Genome annotation databases

EnsemblPlantsiAT2G01630.1; AT2G01630.1; AT2G01630. [Q9ZU91-1]
GeneIDi814692.
GrameneiAT2G01630.1; AT2G01630.1; AT2G01630.
KEGGiath:AT2G01630.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006069 Genomic DNA. Translation: AAD12708.2.
CP002685 Genomic DNA. Translation: AEC05475.1.
AY085500 mRNA. Translation: AAM62724.1.
PIRiB84427.
RefSeqiNP_565269.1. NM_126224.2. [Q9ZU91-1]
UniGeneiAt.27628.
At.42475.
At.75580.

3D structure databases

ProteinModelPortaliQ9ZU91.
SMRiQ9ZU91.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM43. Carbohydrate-Binding Module Family 43.
GH17. Glycoside Hydrolase Family 17.

Proteomic databases

PaxDbiQ9ZU91.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G01630.1; AT2G01630.1; AT2G01630. [Q9ZU91-1]
GeneIDi814692.
GrameneiAT2G01630.1; AT2G01630.1; AT2G01630.
KEGGiath:AT2G01630.

Organism-specific databases

TAIRiAT2G01630.

Phylogenomic databases

eggNOGiENOG410IEQ4. Eukaryota.
ENOG410ZF4V. LUCA.
HOGENOMiHOG000238220.
InParanoidiQ9ZU91.
KOiK19891.
OMAiPLQANKE.
OrthoDBiEOG093607VJ.
PhylomeDBiQ9ZU91.

Enzyme and pathway databases

BioCyciARA:AT2G01630-MONOMER.

Miscellaneous databases

PROiQ9ZU91.

Gene expression databases

ExpressionAtlasiQ9ZU91. baseline and differential.
GenevisibleiQ9ZU91. AT.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
PF07983. X8. 1 hit.
[Graphical view]
SMARTiSM00768. X8. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiE133_ARATH
AccessioniPrimary (citable) accession number: Q9ZU91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: June 1, 2002
Last modified: November 2, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.