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Q9ZU91

- E133_ARATH

UniProt

Q9ZU91 - E133_ARATH

Protein

Glucan endo-1,3-beta-glucosidase 3

Gene

At2g01630

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei263 – 2631NucleophileBy similarity
    Active sitei326 – 3261Proton donorBy similarity

    GO - Molecular functioni

    1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. defense response Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Plant defense

    Enzyme and pathway databases

    BioCyciARA:AT2G01630-MONOMER.
    ARA:GQT-2626-MONOMER.

    Protein family/group databases

    CAZyiCBM43. Carbohydrate-Binding Module Family 43.
    GH17. Glycoside Hydrolase Family 17.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucan endo-1,3-beta-glucosidase 3 (EC:3.2.1.39)
    Alternative name(s):
    (1->3)-beta-glucan endohydrolase 3
    Short name:
    (1->3)-beta-glucanase 3
    Beta-1,3-endoglucanase 3
    Short name:
    Beta-1,3-glucanase 3
    Gene namesi
    Ordered Locus Names:At2g01630
    ORF Names:T8O11.20
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G01630.

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of membrane Source: TAIR
    2. anchored component of plasma membrane Source: TAIR
    3. plasma membrane Source: TAIR

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 483465Glucan endo-1,3-beta-glucosidase 3PRO_0000011886Add
    BLAST
    Propeptidei484 – 50118Removed in mature formSequence AnalysisPRO_0000011887Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi88 – 881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi107 – 1071N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi171 – 1711N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi253 – 2531N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi295 – 2951N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi353 – 3531N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi357 – 3571N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi361 ↔ 424By similarity
    Glycosylationi451 – 4511N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi456 – 4561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi457 – 4571N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi466 – 4661N-linked (GlcNAc...)Sequence Analysis
    Lipidationi483 – 4831GPI-anchor amidated serineSequence Analysis

    Post-translational modificationi

    Contains two additional disulfide bonds.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PaxDbiQ9ZU91.
    PRIDEiQ9ZU91.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9ZU91.
    GenevestigatoriQ9ZU91.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ZU91.
    SMRiQ9ZU91. Positions 23-342, 359-448.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 17 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG290245.
    HOGENOMiHOG000238220.
    InParanoidiQ9ZU91.
    OMAiRNVAAHY.
    PhylomeDBiQ9ZU91.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR012946. X8.
    [Graphical view]
    PfamiPF00332. Glyco_hydro_17. 1 hit.
    PF07983. X8. 1 hit.
    [Graphical view]
    SMARTiSM00768. X8. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q9ZU91-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAALLLLFLF LFASSALSQD SLIGVNIGTE VTNMPSPTQV VALLKSQNIN    50
    RVRLYDADRS MLLAFAHTGV QVIISVPNDQ LLGISQSNAT AANWVTRNVA 100
    AYYPATNITT IAVGSEVLTS LTNAASVLVS ALKYIQAALV TANLDRQIKV 150
    STPHSSTIIL DSFPPSQAFF NKTWDPVIVP LLKFLQSTGS PLLLNVYPYF 200
    DYVQSNGVIP LDYALFQPLQ ANKEAVDANT LLHYTNVFDA IVDAAYFAMS 250
    YLNFTNIPIV VTESGWPSKG GPSEHDATVE NANTYNSNLI QHVINKTGTP 300
    KHPGTAVTTY IYELYNEDTR PGPVSEKNWG LFYTNGTPVY TLRLAGAGAI 350
    LANDTTNQTF CIAKEKVDRK MLQAALDWAC GPGKVDCSAL MQGESCYEPD 400
    DVVAHSTYAF NAYYQKMGKA SGSCDFKGVA TVTTTDPSRG TCVFPGSAKS 450
    NQTLGNNTSA LAPSANSTTS GCIPKYYHHP HASFGDLTLL SLLLIIALVF 500
    L 501
    Length:501
    Mass (Da):53,992
    Last modified:June 1, 2002 - v2
    Checksum:i3858B1C25FD37920
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006069 Genomic DNA. Translation: AAD12708.2.
    CP002685 Genomic DNA. Translation: AEC05475.1.
    AY085500 mRNA. Translation: AAM62724.1.
    PIRiB84427.
    RefSeqiNP_565269.1. NM_126224.2. [Q9ZU91-1]
    UniGeneiAt.27628.
    At.42475.
    At.75580.

    Genome annotation databases

    EnsemblPlantsiAT2G01630.1; AT2G01630.1; AT2G01630. [Q9ZU91-1]
    GeneIDi814692.
    KEGGiath:AT2G01630.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006069 Genomic DNA. Translation: AAD12708.2 .
    CP002685 Genomic DNA. Translation: AEC05475.1 .
    AY085500 mRNA. Translation: AAM62724.1 .
    PIRi B84427.
    RefSeqi NP_565269.1. NM_126224.2. [Q9ZU91-1 ]
    UniGenei At.27628.
    At.42475.
    At.75580.

    3D structure databases

    ProteinModelPortali Q9ZU91.
    SMRi Q9ZU91. Positions 23-342, 359-448.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM43. Carbohydrate-Binding Module Family 43.
    GH17. Glycoside Hydrolase Family 17.

    Proteomic databases

    PaxDbi Q9ZU91.
    PRIDEi Q9ZU91.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G01630.1 ; AT2G01630.1 ; AT2G01630 . [Q9ZU91-1 ]
    GeneIDi 814692.
    KEGGi ath:AT2G01630.

    Organism-specific databases

    TAIRi AT2G01630.

    Phylogenomic databases

    eggNOGi NOG290245.
    HOGENOMi HOG000238220.
    InParanoidi Q9ZU91.
    OMAi RNVAAHY.
    PhylomeDBi Q9ZU91.

    Enzyme and pathway databases

    BioCyci ARA:AT2G01630-MONOMER.
    ARA:GQT-2626-MONOMER.

    Gene expression databases

    ArrayExpressi Q9ZU91.
    Genevestigatori Q9ZU91.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR012946. X8.
    [Graphical view ]
    Pfami PF00332. Glyco_hydro_17. 1 hit.
    PF07983. X8. 1 hit.
    [Graphical view ]
    SMARTi SM00768. X8. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "Identification of glycosylphosphatidylinositol-anchored proteins in Arabidopsis. A proteomic and genomic analysis."
      Borner G.H.H., Lilley K.S., Stevens T.J., Dupree P.
      Plant Physiol. 132:568-577(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].
      Strain: cv. Columbia.
      Tissue: Callus.
    5. "Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins."
      Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C., Jensen O.N.
      Mol. Cell. Proteomics 2:1261-1270(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].
    6. "Modification-specific proteomics of plasma membrane proteins: identification and characterization of glycosylphosphatidylinositol-anchored proteins released upon phospholipase D treatment."
      Elortza F., Mohammed S., Bunkenborg J., Foster L.J., Nuehse T.S., Brodbeck U., Peck S.C., Jensen O.N.
      J. Proteome Res. 5:935-943(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiE133_ARATH
    AccessioniPrimary (citable) accession number: Q9ZU91
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2003
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3