Q9ZU90 (SKI28_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
F-box protein SKIP28

Alternative name(s):
Protein MATERNAL EFFECT EMBRYO ARREST 11
SKP1-interacting partner 28

Gene names

Name:	SKIP28
Synonyms:	MEE11
Ordered Locus Names:	At2g01620
ORF Names:	T8O11.21

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins By similarity. Required during the endosperm development in embryos. Ref.7
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Part of a SCF (ASK-cullin-F-box) protein ligase complex By similarity. Interacts with SKP1A/ASK1 and CUL1. Ref.6
Domain	The F-box is necessary for the interaction with ASK proteins By similarity.
Sequence similarities	Contains 1 F-box domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	embryo development ending in seed dormancy Inferred from mutant phenotype Ref.7. Source: TAIR
Molecular function	protein binding Inferred from physical interaction Ref.6. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SKP1A	Q39255	3	EBI-604427,EBI-532357

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	F-box protein SKIP28		PRO_0000375240
Regions
	Domain	21 – 79	59	F-box; degenerate
	Compositional bias	173 – 251	79	Cys-rich
Experimental info
	Sequence conflict	75 – 76	2	FS → IT in AAM60922. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q9ZU90 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 250E7397BF69569A	FASTA	292	33,005
	10 20 30 40 50 60 MKTELEEEEE EEWRSVHEVL LIVLPYLHSL FELLSMIRVS RSLRDAIRDE TALWTKLVIE 70 80 90 100 110 120 PPLSSRLTDD ILSEFSSKSA GKLKTLILRQ CLMVTNKGLR RVVDANPLIT KIIVPGCSGL 130 140 150 160 170 180 TPEGIMECVE SLSKNNHKLE TLHINGVNGF TKQHLSALYT YLSSEGTIDL EVCPKCDEVR 190 200 210 220 230 240 MIPSCSRESC NQKQRKCRGC WLCIPRCAEC AVCLVGSDTE SQEAACGNDD VLCLECWLVL 250 260 270 280 290 PKCRFCNKPY CTNHSSRRHE IAITDAASRP SFECEACYYR AGTNPYEVDY QI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed: 11910074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis." Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J., Hellmann H., Estelle M., Somers D.E., Crosby W.L. Plant J. 34:753-767(2003) [PubMed: 12795696] [Abstract] Cited for: INTERACTION WITH SKP1A/ASK1 AND CUL1.
[7]	"Genetic and molecular identification of genes required for female gametophyte development and function in Arabidopsis." Pagnussat G.C., Yu H.-J., Ngo Q.A., Rajani S., Mayalagu S., Johnson C.S., Capron A., Xie L.-F., Ye D., Sundaresan V. Development 132:603-614(2005) [PubMed: 15634699] [Abstract] Cited for: FUNCTION.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC006069 Genomic DNA. Translation: AAD12709.1. CP002685 Genomic DNA. Translation: AEC05474.1. AK117653 mRNA. Translation: BAC42307.1. BT005175 mRNA. Translation: AAO50708.1. AY084339 mRNA. Translation: AAM60922.1.
IPI	IPI00533439.
PIR	A84427.
RefSeq	NP_565268.1. NM_126223.2.
UniGene	At.42475. At.75112.
3D structure databases
ProteinModelPortal	Q9ZU90.
SMR	Q9ZU90. Positions 14-58, 83-174.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9ZU90. 10 interactions.
STRING	Q9ZU90.
Proteomic databases
PRIDE	Q9ZU90.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G01620.1; AT2G01620.1; AT2G01620.
GeneID	814691.
GenomeReviews	Gene locus AT2G01620 in contig CT485783_GR.
KEGG	ath:AT2G01620.
NMPDR	fig|3702.1.peg.7765.
Organism-specific databases
TAIR	At2g01620.
Phylogenomic databases
GeneTree	EPGT00050000018293.
InParanoid	Q9ZU90.
OMA	IMECVES.
PhylomeDB	Q9ZU90.
ProtClustDB	CLSN2687660.
Gene expression databases
ArrayExpress	Q9ZU90.
Genevestigator	Q9ZU90.
Family and domain databases
PROSITE	PS50181. FBOX. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SKI28_ARATH

Accession

Primary (citable) accession number: Q9ZU90
Secondary accession number(s): Q8LGD0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program