Probable fructose-bisphosphate aldolase 3, chloroplastic precursor

Contribute

Send feedback

Read comments (?) or add your own

Q9ZU52 (ALFC3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 82. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Probable fructose-bisphosphate aldolase 3, chloroplastic
EC=4.1.2.13

Gene names

Name:	FBA3
Ordered Locus Names:	At2g01140
ORF Names:	F10A8.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Subcellular location	Plastid › chloroplast › plastoglobule Ref.6 Ref.7.
Induction	Down-regulated by oxidative stress. Ref.5
Post-translational modification	Can be trimethylated at Lys-387 by LSMT-L, but the trimethylation has no effect in vitro. Ref.8
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Schiff base
Molecular function	Lyase
PTM	Methylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway response to cadmium ion Inferred from expression pattern PubMed 16502469. Source: TAIR response to oxidative stress Inferred from direct assay Ref.5. Source: TAIR
Cellular_component	chloroplast thylakoid Inferred from direct assay PubMed 20061580. Source: TAIR mitochondrion Inferred from direct assay Ref.5 PubMed 14671022 PubMed 18385124. Source: TAIR plastoglobule Inferred from direct assay Ref.6 Ref.7. Source: TAIR
Molecular_function	fructose-bisphosphate aldolase activity Inferred from sequence or structural similarity PubMed 10725559. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 40

Chloroplast Potential

Chain

41 – 391

351

Probable fructose-bisphosphate aldolase 3, chloroplastic

PRO_0000286528

Sites

Active site

218

Proton acceptor By similarity

Active site

260

Schiff-base intermediate with dihydroxyacetone-P By similarity

Binding site

Substrate By similarity

Binding site

178

Substrate By similarity

Site

391

Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate By similarity

Amino acid modifications

Modified residue

387

N6,N6,N6-trimethyllysine Ref.8

Sequences

Sequence

Length

Mass (Da)

Tools

Q9ZU52 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 4512045C1C325D2A
Show »

FASTA

391

42,327

        10         20         30         40         50         60 
MASASFVKPN TLSSPWIGQR SFAHTSASSS PPPRVSFAIR AGAYSDELVK TAKSIASPGR 

        70         80         90        100        110        120 
GILAIDESNA TCGKRLASIG LDNTEDNRQA YRQLLLTTPG LGDYISGSIL FEETLYQSTK 

       130        140        150        160        170        180 
DGKTFVDCLR DANIVPGIKV DKGLSPLAGS NEESWCQGLD GLASRSAEYY KQGARFAKWR 

       190        200        210        220        230        240 
TVVSVPCGPS ALAVKEAAWG LARYAAISQD NGLVPIVEPE ILLDGDHPIE RTLEVAEKVW 

       250        260        270        280        290        300 
SEVFFYLAQN NVMFEGILLK PSMVTPGAEH KNKASPETVA DFTLTMLKRR VPPAVPGIMF 

       310        320        330        340        350        360 
LSGGQSEAEA TLNLNAMNQS PNPWHVSFSY ARALQNSVLR TWQGKPEKIE ASQKALLVRA 

       370        380        390 
KANSLAQLGK YSAEGENEDA KKGMFVKGYT Y

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The impact of oxidative stress on Arabidopsis mitochondria." Sweetlove L.J., Heazlewood J.L., Herald V., Holtzapffel R., Day D.A., Leaver C.J., Millar A.H. Plant J. 32:891-904(2002) [PubMed] [Europe PMC] [Abstract] Cited for: DOWN-REGULATION BY OXIDATIVE STRESS.
[6]	"Tocopherol cyclase (VTE1) localization and vitamin E accumulation in chloroplast plastoglobule lipoprotein particles." Vidi P.-A., Kanwischer M., Baginsky S., Austin J.R., Csucs G., Doermann P., Kessler F., Brehelin C. J. Biol. Chem. 281:11225-11234(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: cv. Col-2.
[7]	"Protein profiling of plastoglobules in chloroplasts and chromoplasts. A surprising site for differential accumulation of metabolic enzymes." Ytterberg A.J., Peltier J.-B., van Wijk K.J. Plant Physiol. 140:984-997(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: cv. Columbia.
[8]	"Characterization of chloroplastic fructose 1,6-bisphosphate aldolases as lysine-methylated proteins in plants." Mininno M., Brugiere S., Pautre V., Gilgen A., Ma S., Ferro M., Tardif M., Alban C., Ravanel S. J. Biol. Chem. 287:21034-21044(2012) [PubMed] [Europe PMC] [Abstract] Cited for: METHYLATION AT LYS-387.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC006200 Genomic DNA. Translation: AAD14543.1. CP002685 Genomic DNA. Translation: AEC05406.1. AF325014 mRNA. Translation: AAG40366.1. AY086203 mRNA. Translation: AAM64281.1.
IPI	IPI00527081.
PIR	B84421.
RefSeq	NP_178224.1. NM_126176.4.
UniGene	At.22588.
3D structure databases
HSSP	HSSP built from PDB template 1XDL based on UniProtKB P05062.
ProteinModelPortal	Q9ZU52.
SMR	Q9ZU52. Positions 45-369.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9ZU52. 1 interaction.
Proteomic databases
PaxDb	Q9ZU52.
PRIDE	Q9ZU52.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G01140.1; AT2G01140.1; AT2G01140.
GeneID	814643.
KEGG	ath:AT2G01140.
Organism-specific databases
TAIR	At2g01140.
Phylogenomic databases
eggNOG	COG3588.
HOGENOM	HOG000220876.
InParanoid	Q9ZU52.
KO	K01623.
OMA	TEPNRQA.
PhylomeDB	Q9ZU52.
ProtClustDB	PLN02425.
Enzyme and pathway databases
UniPathway	UPA00109; UER00183.
Gene expression databases
ArrayExpress	Q9ZU52.
Genevestigator	Q9ZU52.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR000741. Aldolase_I. IPR013785. Aldolase_TIM. [Graphical view]
PANTHER	PTHR11627. PTHR11627. 1 hit.
Pfam	PF00274. Glycolytic. 1 hit. [Graphical view]
PROSITE	PS00158. ALDOLASE_CLASS_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALFC3_ARATH

Accession

Primary (citable) accession number: Q9ZU52

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents