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Q9ZU52 (ALFC3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable fructose-bisphosphate aldolase 3, chloroplastic

EC=4.1.2.13
Gene names
Name:FBA3
Ordered Locus Names:At2g01140
ORF Names:F10A8.2
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length391 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.

Subcellular location

Plastidchloroplastplastoglobule Ref.6 Ref.7.

Induction

Down-regulated by oxidative stress. Ref.5

Post-translational modification

Can be trimethylated at Lys-387 by LSMT-L, but the trimethylation has no effect in vitro. Ref.8

Sequence similarities

Belongs to the class I fructose-bisphosphate aldolase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4040Chloroplast Potential
Chain41 – 391351Probable fructose-bisphosphate aldolase 3, chloroplastic
PRO_0000286528

Sites

Active site2181Proton acceptor By similarity
Active site2601Schiff-base intermediate with dihydroxyacetone-P By similarity
Binding site881Substrate By similarity
Binding site1781Substrate By similarity
Site3911Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate By similarity

Amino acid modifications

Modified residue2081Phosphoserine By similarity
Modified residue3871N6,N6,N6-trimethyllysine Ref.8

Sequences

Sequence LengthMass (Da)Tools
Q9ZU52 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 4512045C1C325D2A

FASTA39142,327
        10         20         30         40         50         60 
MASASFVKPN TLSSPWIGQR SFAHTSASSS PPPRVSFAIR AGAYSDELVK TAKSIASPGR 

        70         80         90        100        110        120 
GILAIDESNA TCGKRLASIG LDNTEDNRQA YRQLLLTTPG LGDYISGSIL FEETLYQSTK 

       130        140        150        160        170        180 
DGKTFVDCLR DANIVPGIKV DKGLSPLAGS NEESWCQGLD GLASRSAEYY KQGARFAKWR 

       190        200        210        220        230        240 
TVVSVPCGPS ALAVKEAAWG LARYAAISQD NGLVPIVEPE ILLDGDHPIE RTLEVAEKVW 

       250        260        270        280        290        300 
SEVFFYLAQN NVMFEGILLK PSMVTPGAEH KNKASPETVA DFTLTMLKRR VPPAVPGIMF 

       310        320        330        340        350        360 
LSGGQSEAEA TLNLNAMNQS PNPWHVSFSY ARALQNSVLR TWQGKPEKIE ASQKALLVRA 

       370        380        390 
KANSLAQLGK YSAEGENEDA KKGMFVKGYT Y 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"The impact of oxidative stress on Arabidopsis mitochondria."
Sweetlove L.J., Heazlewood J.L., Herald V., Holtzapffel R., Day D.A., Leaver C.J., Millar A.H.
Plant J. 32:891-904(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: DOWN-REGULATION BY OXIDATIVE STRESS.
[6]"Tocopherol cyclase (VTE1) localization and vitamin E accumulation in chloroplast plastoglobule lipoprotein particles."
Vidi P.-A., Kanwischer M., Baginsky S., Austin J.R., Csucs G., Doermann P., Kessler F., Brehelin C.
J. Biol. Chem. 281:11225-11234(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: cv. Col-2.
[7]"Protein profiling of plastoglobules in chloroplasts and chromoplasts. A surprising site for differential accumulation of metabolic enzymes."
Ytterberg A.J., Peltier J.-B., van Wijk K.J.
Plant Physiol. 140:984-997(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: cv. Columbia.
[8]"Characterization of chloroplastic fructose 1,6-bisphosphate aldolases as lysine-methylated proteins in plants."
Mininno M., Brugiere S., Pautre V., Gilgen A., Ma S., Ferro M., Tardif M., Alban C., Ravanel S.
J. Biol. Chem. 287:21034-21044(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: METHYLATION AT LYS-387.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC006200 Genomic DNA. Translation: AAD14543.1.
CP002685 Genomic DNA. Translation: AEC05406.1.
AF325014 mRNA. Translation: AAG40366.1.
AY086203 mRNA. Translation: AAM64281.1.
PIRB84421.
RefSeqNP_178224.1. NM_126176.4.
UniGeneAt.22588.

3D structure databases

ProteinModelPortalQ9ZU52.
SMRQ9ZU52. Positions 45-369.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid47. 2 interactions.
IntActQ9ZU52. 1 interaction.

Proteomic databases

PaxDbQ9ZU52.
PRIDEQ9ZU52.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G01140.1; AT2G01140.1; AT2G01140.
GeneID814643.
KEGGath:AT2G01140.

Organism-specific databases

TAIRAT2G01140.

Phylogenomic databases

eggNOGCOG3588.
HOGENOMHOG000220876.
InParanoidQ9ZU52.
KOK01623.
OMAEPNRQAY.
PhylomeDBQ9ZU52.

Enzyme and pathway databases

BioCycARA:AT2G01140-MONOMER.
UniPathwayUPA00109; UER00183.

Gene expression databases

GenevestigatorQ9ZU52.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR000741. Aldolase_I.
IPR013785. Aldolase_TIM.
[Graphical view]
PANTHERPTHR11627. PTHR11627. 1 hit.
PfamPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameALFC3_ARATH
AccessionPrimary (citable) accession number: Q9ZU52
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 1, 1999
Last modified: June 11, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names