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Protein

Fructose-bisphosphate aldolase 3, chloroplastic

Gene

FBA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a key role in glycolysis and gluconeogenesis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.Curated
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (AXX17_At5g40630), Glucose-6-phosphate isomerase (PGI1 (At4g24620)), Glucose-6-phosphate isomerase, cytosolic (PGIC), Glucose-6-phosphate isomerase (AXX17_At4g28500), Glucose-6-phosphate isomerase 1, chloroplastic (PGI1), Glucose-6-phosphate isomerase (PGIC)
  3. ATP-dependent 6-phosphofructokinase 1 (PFK1), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase 3 (PFK3), ATP-dependent 6-phosphofructokinase 4, chloroplastic (PFK4), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase 2 (PFK2), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 2 (PFP-BETA2), ATP-dependent 6-phosphofructokinase 5, chloroplastic (PFK5), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 1 (PFP-ALPHA1), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), ATP-dependent 6-phosphofructokinase 6 (PFK6), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 2 (PFP-ALPHA2), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 1 (PFP-BETA1), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase 7 (PFK7), ATP-dependent 6-phosphofructokinase (PFK)
  4. Fructose-bisphosphate aldolase 5, cytosolic (FBA5), Fructose-bisphosphate aldolase (At2g36460), Fructose-bisphosphate aldolase (AXX17_At4g44320), Fructose-bisphosphate aldolase (AXX17_At4g30610), Fructose-bisphosphate aldolase (FBA2), Fructose-bisphosphate aldolase 1, chloroplastic (FBA1), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 7, cytosolic (FBA7), Fructose-bisphosphate aldolase 6, cytosolic (FAB6), Fructose-bisphosphate aldolase 3, chloroplastic (FBA3), Fructose-bisphosphate aldolase (AXX17_At2g00100), Fructose-bisphosphate aldolase 2, chloroplastic (FBA2), Fructose-bisphosphate aldolase (At2g21330), Fructose-bisphosphate aldolase (AXX17_At2g33200), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (AXX17_At3g47340), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (AXX17_At2g16800), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 8, cytosolic (FBA8), Fructose-bisphosphate aldolase (AXX17_At4g30600), Fructose-bisphosphate aldolase (AXX17_At3g47340), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 4, cytosolic (FBA4)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei88SubstrateBy similarity1
Binding sitei178SubstrateBy similarity1
Active sitei218Proton acceptorBy similarity1
Active sitei260Schiff-base intermediate with dihydroxyacetone-PBy similarity1
Sitei391Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphateBy similarity1

GO - Molecular functioni

  • fructose-bisphosphate aldolase activity Source: TAIR

GO - Biological processi

  • glycolytic process Source: UniProtKB-UniPathway
  • response to cadmium ion Source: TAIR
  • response to oxidative stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

BioCyciARA:AT2G01140-MONOMER.
UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase 3, chloroplasticCurated (EC:4.1.2.13Curated)
Short name:
AtFBA31 Publication
Alternative name(s):
Protein PIGMENT DEFECTIVE 345
Gene namesi
Name:FBA31 Publication
Synonyms:PDE345
Ordered Locus Names:At2g01140
ORF Names:F10A8.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G01140.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
  • chloroplast thylakoid Source: TAIR
  • mitochondrion Source: TAIR
  • plastid Source: TAIR
  • plastoglobule Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 40ChloroplastSequence analysisAdd BLAST40
ChainiPRO_000028652841 – 391Fructose-bisphosphate aldolase 3, chloroplasticAdd BLAST351

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei150PhosphoserineBy similarity1
Modified residuei208PhosphoserineBy similarity1
Modified residuei387N6,N6,N6-trimethyllysine1 Publication1

Post-translational modificationi

Can be trimethylated at Lys-387 by LSMT-L, but the trimethylation has no effect in vitro.1 Publication
S-glutathionylated.1 Publication

Keywords - PTMi

Glutathionylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ9ZU52.
PRIDEiQ9ZU52.

PTM databases

iPTMnetiQ9ZU52.

Expressioni

Tissue specificityi

Expressed in roots, and at low levels in rosettes leaves, cauline leaves, stems and flowers.1 Publication

Inductioni

Down-regulated by oxidative stress (PubMed:12492832). Induced by fructose and sucrose (PubMed:22561114). Doen-regulated by abiotic stresses (PubMed:22561114).2 Publications

Gene expression databases

GenevisibleiQ9ZU52. AT.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi47. 2 interactors.
IntActiQ9ZU52. 1 interactor.
STRINGi3702.AT2G01140.1.

Structurei

3D structure databases

ProteinModelPortaliQ9ZU52.
SMRiQ9ZU52.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni302 – 304Substrate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1557. Eukaryota.
COG3588. LUCA.
HOGENOMiHOG000220876.
InParanoidiQ9ZU52.
KOiK01623.
OMAiSWIGQQS.
OrthoDBiEOG09360CPA.
PhylomeDBiQ9ZU52.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR029768. Aldolase_I_AS.
IPR013785. Aldolase_TIM.
IPR000741. FBA_I.
[Graphical view]
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ZU52-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASASFVKPN TLSSPWIGQR SFAHTSASSS PPPRVSFAIR AGAYSDELVK
60 70 80 90 100
TAKSIASPGR GILAIDESNA TCGKRLASIG LDNTEDNRQA YRQLLLTTPG
110 120 130 140 150
LGDYISGSIL FEETLYQSTK DGKTFVDCLR DANIVPGIKV DKGLSPLAGS
160 170 180 190 200
NEESWCQGLD GLASRSAEYY KQGARFAKWR TVVSVPCGPS ALAVKEAAWG
210 220 230 240 250
LARYAAISQD NGLVPIVEPE ILLDGDHPIE RTLEVAEKVW SEVFFYLAQN
260 270 280 290 300
NVMFEGILLK PSMVTPGAEH KNKASPETVA DFTLTMLKRR VPPAVPGIMF
310 320 330 340 350
LSGGQSEAEA TLNLNAMNQS PNPWHVSFSY ARALQNSVLR TWQGKPEKIE
360 370 380 390
ASQKALLVRA KANSLAQLGK YSAEGENEDA KKGMFVKGYT Y
Length:391
Mass (Da):42,327
Last modified:May 1, 1999 - v1
Checksum:i4512045C1C325D2A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006200 Genomic DNA. Translation: AAD14543.1.
CP002685 Genomic DNA. Translation: AEC05406.1.
AF325014 mRNA. Translation: AAG40366.1.
AY086203 mRNA. Translation: AAM64281.1.
PIRiB84421.
RefSeqiNP_178224.1. NM_126176.5.
UniGeneiAt.22588.

Genome annotation databases

EnsemblPlantsiAT2G01140.1; AT2G01140.1; AT2G01140.
GeneIDi814643.
GrameneiAT2G01140.1; AT2G01140.1; AT2G01140.
KEGGiath:AT2G01140.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006200 Genomic DNA. Translation: AAD14543.1.
CP002685 Genomic DNA. Translation: AEC05406.1.
AF325014 mRNA. Translation: AAG40366.1.
AY086203 mRNA. Translation: AAM64281.1.
PIRiB84421.
RefSeqiNP_178224.1. NM_126176.5.
UniGeneiAt.22588.

3D structure databases

ProteinModelPortaliQ9ZU52.
SMRiQ9ZU52.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi47. 2 interactors.
IntActiQ9ZU52. 1 interactor.
STRINGi3702.AT2G01140.1.

PTM databases

iPTMnetiQ9ZU52.

Proteomic databases

PaxDbiQ9ZU52.
PRIDEiQ9ZU52.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G01140.1; AT2G01140.1; AT2G01140.
GeneIDi814643.
GrameneiAT2G01140.1; AT2G01140.1; AT2G01140.
KEGGiath:AT2G01140.

Organism-specific databases

TAIRiAT2G01140.

Phylogenomic databases

eggNOGiKOG1557. Eukaryota.
COG3588. LUCA.
HOGENOMiHOG000220876.
InParanoidiQ9ZU52.
KOiK01623.
OMAiSWIGQQS.
OrthoDBiEOG09360CPA.
PhylomeDBiQ9ZU52.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.
BioCyciARA:AT2G01140-MONOMER.

Miscellaneous databases

PROiQ9ZU52.

Gene expression databases

GenevisibleiQ9ZU52. AT.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR029768. Aldolase_I_AS.
IPR013785. Aldolase_TIM.
IPR000741. FBA_I.
[Graphical view]
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALFP3_ARATH
AccessioniPrimary (citable) accession number: Q9ZU52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.