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Protein

Fructose-bisphosphate aldolase 3, chloroplastic

Gene

FBA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a key role in glycolysis and gluconeogenesis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.Curated
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (MJB21.12), Glucose-6-phosphate isomerase (AXX17_At5g40630), Glucose-6-phosphate isomerase (PGI1 (At4g24620)), Glucose-6-phosphate isomerase, cytosolic (PGIC), Glucose-6-phosphate isomerase (AXX17_At4g28500), Glucose-6-phosphate isomerase 1, chloroplastic (PGI1), Glucose-6-phosphate isomerase (PGIC)
  3. ATP-dependent 6-phosphofructokinase 1 (PFK1), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase 3 (PFK3), ATP-dependent 6-phosphofructokinase 4, chloroplastic (PFK4), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase 2 (PFK2), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 2 (PFP-BETA2), ATP-dependent 6-phosphofructokinase 5, chloroplastic (PFK5), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 1 (PFP-ALPHA1), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), ATP-dependent 6-phosphofructokinase 6 (PFK6), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 2 (PFP-ALPHA2), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 1 (PFP-BETA1), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase 7 (PFK7), ATP-dependent 6-phosphofructokinase (PFK)
  4. Fructose-bisphosphate aldolase 5, cytosolic (FBA5), Fructose-bisphosphate aldolase (FBA6), Fructose-bisphosphate aldolase (FBA4), Fructose-bisphosphate aldolase (AXX17_At4g44320), Fructose-bisphosphate aldolase (AXX17_At4g30610), Fructose-bisphosphate aldolase (FBA2), Fructose-bisphosphate aldolase 1, chloroplastic (FBA1), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 7, cytosolic (FBA7), Fructose-bisphosphate aldolase 6, cytosolic (FBA6), Fructose-bisphosphate aldolase 3, chloroplastic (FBA3), Fructose-bisphosphate aldolase (FBA7), Fructose-bisphosphate aldolase (AXX17_At2g00100), Fructose-bisphosphate aldolase 2, chloroplastic (FBA2), Fructose-bisphosphate aldolase (At2g21330), Fructose-bisphosphate aldolase (AXX17_At2g33200), Fructose-bisphosphate aldolase (AXX17_At3g47340), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (AXX17_At2g16800), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 8, cytosolic (FBA8), Fructose-bisphosphate aldolase (FBA7), Fructose-bisphosphate aldolase (AXX17_At4g30600), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase (AXX17_At3g47340), Fructose-bisphosphate aldolase 4, cytosolic (FBA4)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei88SubstrateBy similarity1
Binding sitei178SubstrateBy similarity1
Active sitei218Proton acceptorBy similarity1
Active sitei260Schiff-base intermediate with dihydroxyacetone-PBy similarity1
Sitei391Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphateBy similarity1

GO - Molecular functioni

  • fructose-bisphosphate aldolase activity Source: UniProtKB

GO - Biological processi

  • gluconeogenesis Source: UniProtKB
  • glycolytic process Source: UniProtKB
  • response to cadmium ion Source: TAIR
  • response to oxidative stress Source: TAIR

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandSchiff base

Enzyme and pathway databases

BioCyciARA:AT2G01140-MONOMER
UniPathwayiUPA00109; UER00183

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase 3, chloroplasticCurated (EC:4.1.2.13Curated)
Short name:
AtFBA31 Publication
Alternative name(s):
Protein PIGMENT DEFECTIVE 345
Gene namesi
Name:FBA31 Publication
Synonyms:PDE345
Ordered Locus Names:At2g01140
ORF Names:F10A8.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G01140
TAIRilocus:2038726 AT2G01140

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 40ChloroplastSequence analysisAdd BLAST40
ChainiPRO_000028652841 – 391Fructose-bisphosphate aldolase 3, chloroplasticAdd BLAST351

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei150PhosphoserineBy similarity1
Modified residuei208PhosphoserineBy similarity1
Modified residuei387N6,N6,N6-trimethyllysine1 Publication1

Post-translational modificationi

Can be trimethylated at Lys-387 by LSMT-L, but the trimethylation has no effect in vitro.1 Publication
S-glutathionylated.1 Publication

Keywords - PTMi

Glutathionylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ9ZU52
PRIDEiQ9ZU52

PTM databases

iPTMnetiQ9ZU52

Expressioni

Tissue specificityi

Expressed in roots, and at low levels in rosettes leaves, cauline leaves, stems and flowers.1 Publication

Inductioni

Down-regulated by oxidative stress (PubMed:12492832). Induced by fructose and sucrose (PubMed:22561114). Doen-regulated by abiotic stresses (PubMed:22561114).2 Publications

Gene expression databases

ExpressionAtlasiQ9ZU52 baseline and differential
GenevisibleiQ9ZU52 AT

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi47, 2 interactors
IntActiQ9ZU52, 1 interactor
STRINGi3702.AT2G01140.1

Structurei

3D structure databases

ProteinModelPortaliQ9ZU52
SMRiQ9ZU52
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni302 – 304Substrate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1557 Eukaryota
COG3588 LUCA
HOGENOMiHOG000220876
InParanoidiQ9ZU52
KOiK01623
OMAiSWIGQQS
OrthoDBiEOG09360CPA
PhylomeDBiQ9ZU52

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR029768 Aldolase_I_AS
IPR013785 Aldolase_TIM
IPR000741 FBA_I
PfamiView protein in Pfam
PF00274 Glycolytic, 1 hit
PROSITEiView protein in PROSITE
PS00158 ALDOLASE_CLASS_I, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ZU52-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASASFVKPN TLSSPWIGQR SFAHTSASSS PPPRVSFAIR AGAYSDELVK
60 70 80 90 100
TAKSIASPGR GILAIDESNA TCGKRLASIG LDNTEDNRQA YRQLLLTTPG
110 120 130 140 150
LGDYISGSIL FEETLYQSTK DGKTFVDCLR DANIVPGIKV DKGLSPLAGS
160 170 180 190 200
NEESWCQGLD GLASRSAEYY KQGARFAKWR TVVSVPCGPS ALAVKEAAWG
210 220 230 240 250
LARYAAISQD NGLVPIVEPE ILLDGDHPIE RTLEVAEKVW SEVFFYLAQN
260 270 280 290 300
NVMFEGILLK PSMVTPGAEH KNKASPETVA DFTLTMLKRR VPPAVPGIMF
310 320 330 340 350
LSGGQSEAEA TLNLNAMNQS PNPWHVSFSY ARALQNSVLR TWQGKPEKIE
360 370 380 390
ASQKALLVRA KANSLAQLGK YSAEGENEDA KKGMFVKGYT Y
Length:391
Mass (Da):42,327
Last modified:May 1, 1999 - v1
Checksum:i4512045C1C325D2A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006200 Genomic DNA Translation: AAD14543.1
CP002685 Genomic DNA Translation: AEC05406.1
AF325014 mRNA Translation: AAG40366.1
AY086203 mRNA Translation: AAM64281.1
PIRiB84421
RefSeqiNP_178224.1, NM_126176.5
UniGeneiAt.22588

Genome annotation databases

EnsemblPlantsiAT2G01140.1; AT2G01140.1; AT2G01140
GeneIDi814643
GrameneiAT2G01140.1; AT2G01140.1; AT2G01140
KEGGiath:AT2G01140

Similar proteinsi

Entry informationi

Entry nameiALFP3_ARATH
AccessioniPrimary (citable) accession number: Q9ZU52
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 1, 1999
Last modified: April 25, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health