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Q9ZU52

- ALFC3_ARATH

UniProt

Q9ZU52 - ALFC3_ARATH

Protein

Probable fructose-bisphosphate aldolase 3, chloroplastic

Gene

FBA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei88 – 881SubstrateBy similarity
    Binding sitei178 – 1781SubstrateBy similarity
    Active sitei218 – 2181Proton acceptorBy similarity
    Active sitei260 – 2601Schiff-base intermediate with dihydroxyacetone-PBy similarity
    Sitei391 – 3911Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphateBy similarity

    GO - Molecular functioni

    1. fructose-bisphosphate aldolase activity Source: TAIR

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway
    2. response to cadmium ion Source: TAIR
    3. response to oxidative stress Source: TAIR

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Schiff base

    Enzyme and pathway databases

    BioCyciARA:AT2G01140-MONOMER.
    UniPathwayiUPA00109; UER00183.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable fructose-bisphosphate aldolase 3, chloroplastic (EC:4.1.2.13)
    Gene namesi
    Name:FBA3
    Ordered Locus Names:At2g01140
    ORF Names:F10A8.2
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G01140.

    Subcellular locationi

    Plastidchloroplastplastoglobule 2 Publications

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast stroma Source: TAIR
    3. chloroplast thylakoid Source: TAIR
    4. mitochondrion Source: TAIR
    5. plastid Source: TAIR
    6. plastoglobule Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4040ChloroplastSequence AnalysisAdd
    BLAST
    Chaini41 – 391351Probable fructose-bisphosphate aldolase 3, chloroplasticPRO_0000286528Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei208 – 2081PhosphoserineBy similarity
    Modified residuei387 – 3871N6,N6,N6-trimethyllysine1 Publication

    Post-translational modificationi

    Can be trimethylated at Lys-387 by LSMT-L, but the trimethylation has no effect in vitro.

    Keywords - PTMi

    Methylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ9ZU52.
    PRIDEiQ9ZU52.

    Expressioni

    Inductioni

    Down-regulated by oxidative stress.

    Gene expression databases

    GenevestigatoriQ9ZU52.

    Interactioni

    Protein-protein interaction databases

    BioGridi47. 2 interactions.
    IntActiQ9ZU52. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ZU52.
    SMRiQ9ZU52. Positions 45-369.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG3588.
    HOGENOMiHOG000220876.
    InParanoidiQ9ZU52.
    KOiK01623.
    OMAiEPNRQAY.
    PhylomeDBiQ9ZU52.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR000741. FBA_I.
    [Graphical view]
    PfamiPF00274. Glycolytic. 1 hit.
    [Graphical view]
    PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9ZU52-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASASFVKPN TLSSPWIGQR SFAHTSASSS PPPRVSFAIR AGAYSDELVK    50
    TAKSIASPGR GILAIDESNA TCGKRLASIG LDNTEDNRQA YRQLLLTTPG 100
    LGDYISGSIL FEETLYQSTK DGKTFVDCLR DANIVPGIKV DKGLSPLAGS 150
    NEESWCQGLD GLASRSAEYY KQGARFAKWR TVVSVPCGPS ALAVKEAAWG 200
    LARYAAISQD NGLVPIVEPE ILLDGDHPIE RTLEVAEKVW SEVFFYLAQN 250
    NVMFEGILLK PSMVTPGAEH KNKASPETVA DFTLTMLKRR VPPAVPGIMF 300
    LSGGQSEAEA TLNLNAMNQS PNPWHVSFSY ARALQNSVLR TWQGKPEKIE 350
    ASQKALLVRA KANSLAQLGK YSAEGENEDA KKGMFVKGYT Y 391
    Length:391
    Mass (Da):42,327
    Last modified:May 1, 1999 - v1
    Checksum:i4512045C1C325D2A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006200 Genomic DNA. Translation: AAD14543.1.
    CP002685 Genomic DNA. Translation: AEC05406.1.
    AF325014 mRNA. Translation: AAG40366.1.
    AY086203 mRNA. Translation: AAM64281.1.
    PIRiB84421.
    RefSeqiNP_178224.1. NM_126176.4.
    UniGeneiAt.22588.

    Genome annotation databases

    EnsemblPlantsiAT2G01140.1; AT2G01140.1; AT2G01140.
    GeneIDi814643.
    KEGGiath:AT2G01140.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006200 Genomic DNA. Translation: AAD14543.1 .
    CP002685 Genomic DNA. Translation: AEC05406.1 .
    AF325014 mRNA. Translation: AAG40366.1 .
    AY086203 mRNA. Translation: AAM64281.1 .
    PIRi B84421.
    RefSeqi NP_178224.1. NM_126176.4.
    UniGenei At.22588.

    3D structure databases

    ProteinModelPortali Q9ZU52.
    SMRi Q9ZU52. Positions 45-369.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 47. 2 interactions.
    IntActi Q9ZU52. 1 interaction.

    Proteomic databases

    PaxDbi Q9ZU52.
    PRIDEi Q9ZU52.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G01140.1 ; AT2G01140.1 ; AT2G01140 .
    GeneIDi 814643.
    KEGGi ath:AT2G01140.

    Organism-specific databases

    TAIRi AT2G01140.

    Phylogenomic databases

    eggNOGi COG3588.
    HOGENOMi HOG000220876.
    InParanoidi Q9ZU52.
    KOi K01623.
    OMAi EPNRQAY.
    PhylomeDBi Q9ZU52.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00183 .
    BioCyci ARA:AT2G01140-MONOMER.

    Gene expression databases

    Genevestigatori Q9ZU52.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR000741. FBA_I.
    [Graphical view ]
    Pfami PF00274. Glycolytic. 1 hit.
    [Graphical view ]
    PROSITEi PS00158. ALDOLASE_CLASS_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. Cited for: DOWN-REGULATION BY OXIDATIVE STRESS.
    6. "Tocopherol cyclase (VTE1) localization and vitamin E accumulation in chloroplast plastoglobule lipoprotein particles."
      Vidi P.-A., Kanwischer M., Baginsky S., Austin J.R., Csucs G., Doermann P., Kessler F., Brehelin C.
      J. Biol. Chem. 281:11225-11234(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: cv. Col-2.
    7. "Protein profiling of plastoglobules in chloroplasts and chromoplasts. A surprising site for differential accumulation of metabolic enzymes."
      Ytterberg A.J., Peltier J.-B., van Wijk K.J.
      Plant Physiol. 140:984-997(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: cv. Columbia.
    8. "Characterization of chloroplastic fructose 1,6-bisphosphate aldolases as lysine-methylated proteins in plants."
      Mininno M., Brugiere S., Pautre V., Gilgen A., Ma S., Ferro M., Tardif M., Alban C., Ravanel S.
      J. Biol. Chem. 287:21034-21044(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: METHYLATION AT LYS-387.

    Entry informationi

    Entry nameiALFC3_ARATH
    AccessioniPrimary (citable) accession number: Q9ZU52
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 15, 2007
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3