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Protein

Probable fructose-bisphosphate aldolase 3, chloroplastic

Gene

FBA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei88 – 881SubstrateBy similarity
Binding sitei178 – 1781SubstrateBy similarity
Active sitei218 – 2181Proton acceptorBy similarity
Active sitei260 – 2601Schiff-base intermediate with dihydroxyacetone-PBy similarity
Sitei391 – 3911Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphateBy similarity

GO - Molecular functioni

  1. fructose-bisphosphate aldolase activity Source: TAIR

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
  2. response to cadmium ion Source: TAIR
  3. response to oxidative stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

BioCyciARA:AT2G01140-MONOMER.
UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable fructose-bisphosphate aldolase 3, chloroplastic (EC:4.1.2.13)
Gene namesi
Name:FBA3
Ordered Locus Names:At2g01140
ORF Names:F10A8.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G01140.

Subcellular locationi

Plastidchloroplastplastoglobule 2 Publications

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast stroma Source: TAIR
  3. chloroplast thylakoid Source: TAIR
  4. mitochondrion Source: TAIR
  5. plastid Source: TAIR
  6. plastoglobule Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4040ChloroplastSequence AnalysisAdd
BLAST
Chaini41 – 391351Probable fructose-bisphosphate aldolase 3, chloroplasticPRO_0000286528Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei208 – 2081PhosphoserineBy similarity
Modified residuei387 – 3871N6,N6,N6-trimethyllysine1 Publication

Post-translational modificationi

Can be trimethylated at Lys-387 by LSMT-L, but the trimethylation has no effect in vitro.

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ9ZU52.
PRIDEiQ9ZU52.

Expressioni

Inductioni

Down-regulated by oxidative stress.

Gene expression databases

ExpressionAtlasiQ9ZU52. baseline and differential.
GenevestigatoriQ9ZU52.

Interactioni

Protein-protein interaction databases

BioGridi47. 2 interactions.
IntActiQ9ZU52. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9ZU52.
SMRiQ9ZU52. Positions 45-371.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG3588.
HOGENOMiHOG000220876.
InParanoidiQ9ZU52.
KOiK01623.
OMAiSWIGQQS.
PhylomeDBiQ9ZU52.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR029768. Aldolase_I_AS.
IPR013785. Aldolase_TIM.
IPR029769. FBA_euk-type.
IPR000741. FBA_I.
[Graphical view]
PANTHERiPTHR11627. PTHR11627. 1 hit.
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ZU52-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASASFVKPN TLSSPWIGQR SFAHTSASSS PPPRVSFAIR AGAYSDELVK
60 70 80 90 100
TAKSIASPGR GILAIDESNA TCGKRLASIG LDNTEDNRQA YRQLLLTTPG
110 120 130 140 150
LGDYISGSIL FEETLYQSTK DGKTFVDCLR DANIVPGIKV DKGLSPLAGS
160 170 180 190 200
NEESWCQGLD GLASRSAEYY KQGARFAKWR TVVSVPCGPS ALAVKEAAWG
210 220 230 240 250
LARYAAISQD NGLVPIVEPE ILLDGDHPIE RTLEVAEKVW SEVFFYLAQN
260 270 280 290 300
NVMFEGILLK PSMVTPGAEH KNKASPETVA DFTLTMLKRR VPPAVPGIMF
310 320 330 340 350
LSGGQSEAEA TLNLNAMNQS PNPWHVSFSY ARALQNSVLR TWQGKPEKIE
360 370 380 390
ASQKALLVRA KANSLAQLGK YSAEGENEDA KKGMFVKGYT Y
Length:391
Mass (Da):42,327
Last modified:May 1, 1999 - v1
Checksum:i4512045C1C325D2A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006200 Genomic DNA. Translation: AAD14543.1.
CP002685 Genomic DNA. Translation: AEC05406.1.
AF325014 mRNA. Translation: AAG40366.1.
AY086203 mRNA. Translation: AAM64281.1.
PIRiB84421.
RefSeqiNP_178224.1. NM_126176.4.
UniGeneiAt.22588.

Genome annotation databases

EnsemblPlantsiAT2G01140.1; AT2G01140.1; AT2G01140.
GeneIDi814643.
KEGGiath:AT2G01140.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006200 Genomic DNA. Translation: AAD14543.1.
CP002685 Genomic DNA. Translation: AEC05406.1.
AF325014 mRNA. Translation: AAG40366.1.
AY086203 mRNA. Translation: AAM64281.1.
PIRiB84421.
RefSeqiNP_178224.1. NM_126176.4.
UniGeneiAt.22588.

3D structure databases

ProteinModelPortaliQ9ZU52.
SMRiQ9ZU52. Positions 45-371.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi47. 2 interactions.
IntActiQ9ZU52. 1 interaction.

Proteomic databases

PaxDbiQ9ZU52.
PRIDEiQ9ZU52.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G01140.1; AT2G01140.1; AT2G01140.
GeneIDi814643.
KEGGiath:AT2G01140.

Organism-specific databases

TAIRiAT2G01140.

Phylogenomic databases

eggNOGiCOG3588.
HOGENOMiHOG000220876.
InParanoidiQ9ZU52.
KOiK01623.
OMAiSWIGQQS.
PhylomeDBiQ9ZU52.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.
BioCyciARA:AT2G01140-MONOMER.

Gene expression databases

ExpressionAtlasiQ9ZU52. baseline and differential.
GenevestigatoriQ9ZU52.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR029768. Aldolase_I_AS.
IPR013785. Aldolase_TIM.
IPR029769. FBA_euk-type.
IPR000741. FBA_I.
[Graphical view]
PANTHERiPTHR11627. PTHR11627. 1 hit.
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. Cited for: DOWN-REGULATION BY OXIDATIVE STRESS.
  6. "Tocopherol cyclase (VTE1) localization and vitamin E accumulation in chloroplast plastoglobule lipoprotein particles."
    Vidi P.-A., Kanwischer M., Baginsky S., Austin J.R., Csucs G., Doermann P., Kessler F., Brehelin C.
    J. Biol. Chem. 281:11225-11234(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Col-2.
  7. "Protein profiling of plastoglobules in chloroplasts and chromoplasts. A surprising site for differential accumulation of metabolic enzymes."
    Ytterberg A.J., Peltier J.-B., van Wijk K.J.
    Plant Physiol. 140:984-997(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.
  8. "Characterization of chloroplastic fructose 1,6-bisphosphate aldolases as lysine-methylated proteins in plants."
    Mininno M., Brugiere S., Pautre V., Gilgen A., Ma S., Ferro M., Tardif M., Alban C., Ravanel S.
    J. Biol. Chem. 287:21034-21044(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: METHYLATION AT LYS-387.

Entry informationi

Entry nameiALFC3_ARATH
AccessioniPrimary (citable) accession number: Q9ZU52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 1, 1999
Last modified: April 1, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.