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Q9ZTK5

- DAT_CATRO

UniProt

Q9ZTK5 - DAT_CATRO

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Protein
Deacetylvindoline O-acetyltransferase
Gene
DAT
Organism
Catharanthus roseus (Madagascar periwinkle) (Vinca rosea)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of vindoline, a precursor of vinblastine and vincristine.

Catalytic activityi

Acetyl-CoA + deacetylvindoline = CoA + vindoline.

Kineticsi

  1. KM=6.5 mM for acetyl-CoA1 Publication
  2. KM=1.3 mM for deacetylvindoline

Vmax=12.6 pmol/sec/µg enzyme toward acetyl-CoA

Vmax=10.1 pmol/sec/µg enzyme toward deacetylvindoline

pH dependencei

Optimum pH is 7.5-9.0.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei158 – 1581Proton acceptor Reviewed prediction
Active sitei380 – 3801Proton acceptor Reviewed prediction

GO - Molecular functioni

  1. 17-O-deacetylvindoline O-acetyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.3.1.107. 1211.
SABIO-RKQ9ZTK5.
UniPathwayiUPA00365; UER00526.

Names & Taxonomyi

Protein namesi
Recommended name:
Deacetylvindoline O-acetyltransferase (EC:2.3.1.107)
Alternative name(s):
Acetyl-coenzyme A:deacetylvindoline 4-O-acetyltransferase
Gene namesi
Name:DAT
OrganismiCatharanthus roseus (Madagascar periwinkle) (Vinca rosea)
Taxonomic identifieri4058 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesApocynaceaeRauvolfioideaeVinceaeCatharanthinaeCatharanthus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 439439Deacetylvindoline O-acetyltransferase
PRO_0000310727Add
BLAST

Proteomic databases

PRIDEiQ9ZTK5.

Expressioni

Tissue specificityi

Predominantly expressed in young leaves of mature plants. Low expression in stems and flowers and not detected in roots. Expressed in the laticifer and idioblast cells of leaves, stems, and flower buds.2 Publications

Inductioni

Inhibited by tabersonine, coenzyme A, K+, Mg2+ and Mn2+. Induced by light with a maximum 48 hours after initiation of the light treatment.1 Publication

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili312 – 34837 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Family and domain databases

Gene3Di3.30.559.10. 2 hits.
InterProiIPR023213. CAT-like_dom.
IPR003480. Transferase.
[Graphical view]
PfamiPF02458. Transferase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZTK5-1 [UniParc]FASTAAdd to Basket

« Hide

MESGKISVET ETLSKTLIKP SSPTPQSLSR YNLSYNDQNI YQTCVSVGFF    50
YENPDGIEIS TIREQLQNSL SKTLVSYYPF AGKVVKNDYI HCNDDGIEFV 100
EVRIRCRMND ILKYELRSYA RDLVLPKRVT VGSEDTTAIV QLSHFDCGGL 150
AVAFGISHKV ADGGTIASFM KDWAASACYL SSSHHVPTPL LVSDSIFPRQ 200
DNIICEQFPT SKNCVEKTFI FPPEAIEKLK SKAVEFGIEK PTRVEVLTAF 250
LSRCATVAGK SAAKNNNCGQ SLPFPVLQAI NLRPILELPQ NSVGNLVSIY 300
FSRTIKENDY LNEKEYTKLV INELRKEKQK IKNLSREKLT YVAQMEEFVK 350
SLKEFDISNF LDIDAYLSDS WCRFPFYDVD FGWGKPIWVC LFQPYIKNCV 400
VMMDYPFGDD YGIEAIVSFE QEKMSAFEKN EQLLQFVSN 439
Length:439
Mass (Da):49,873
Last modified:May 1, 1999 - v1
Checksum:i1357C76550E0D04D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF053307 Genomic DNA. Translation: AAC99311.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF053307 Genomic DNA. Translation: AAC99311.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q9ZTK5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00365 ; UER00526 .
BRENDAi 2.3.1.107. 1211.
SABIO-RK Q9ZTK5.

Family and domain databases

Gene3Di 3.30.559.10. 2 hits.
InterProi IPR023213. CAT-like_dom.
IPR003480. Transferase.
[Graphical view ]
Pfami PF02458. Transferase. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The terminal O-acetyltransferase involved in vindoline biosynthesis defines a new class of proteins responsible for coenzyme A-dependent acyl transfer."
    St Pierre B., Laflamme P., Alarco A.-M., De Luca V.
    Plant J. 14:703-713(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 6-30; 73-83; 201-216; 232-250; 334-349; 353-373 AND 424-439, INDUCTION, TISSUE SPECIFICITY.
  2. "Purification and characterization of acetylcoenzyme A: deacetylvindoline 4-O-acetyltransferase from Catharanthus roseus."
    Power R., Kurz W.G., De Luca V.
    Arch. Biochem. Biophys. 279:370-376(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, INHIBITION.
  3. "Multicellular compartmentation of Catharanthus roseus alkaloid biosynthesis predicts intercellular translocation of a pathway intermediate."
    St Pierre B., Vazquez-Flota F.A., De Luca V.
    Plant Cell 11:887-900(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiDAT_CATRO
AccessioniPrimary (citable) accession number: Q9ZTK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: May 1, 1999
Last modified: July 9, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Was originally (1 Publication) purified as a heterodimer of the N- and C-terminal end of the DAT gene product, but the cleavage of DAT protein appears to be a purification artifact.

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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