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Q9ZT91

- EFTM_ARATH

UniProt

Q9ZT91 - EFTM_ARATH

Protein

Elongation factor Tu, mitochondrial

Gene

TUFA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi74 – 818GTPBy similarity
    Nucleotide bindingi136 – 1405GTPBy similarity
    Nucleotide bindingi191 – 1944GTPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: TAIR
    2. cobalt ion binding Source: TAIR
    3. GTPase activity Source: InterPro
    4. GTP binding Source: UniProtKB-KW
    5. translation elongation factor activity Source: UniProtKB-KW
    6. zinc ion binding Source: TAIR

    GO - Biological processi

    1. response to cadmium ion Source: TAIR

    Keywords - Molecular functioni

    Elongation factor

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Elongation factor Tu, mitochondrial
    Gene namesi
    Name:TUFA
    Ordered Locus Names:At4g02930
    ORF Names:T4I9.19, T5J8.25
    OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G02930.

    Subcellular locationi

    Mitochondrion 2 Publications

    GO - Cellular componenti

    1. cell wall Source: TAIR
    2. mitochondrion Source: TAIR

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5151MitochondrionSequence AnalysisAdd
    BLAST
    Chaini52 – 454403Elongation factor Tu, mitochondrialPRO_0000007466Add
    BLAST

    Proteomic databases

    PaxDbiQ9ZT91.
    PRIDEiQ9ZT91.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9ZT91.
    GenevestigatoriQ9ZT91.

    Interactioni

    Protein-protein interaction databases

    BioGridi13423. 2 interactions.
    STRINGi3702.AT4G02930.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ZT91.
    SMRiQ9ZT91. Positions 65-453.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini65 – 261197tr-type GAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni74 – 818G1By similarity
    Regioni115 – 1195G2By similarity
    Regioni136 – 1394G3By similarity
    Regioni191 – 1944G4By similarity
    Regioni229 – 2313G5By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0050.
    HOGENOMiHOG000229290.
    InParanoidiQ9ZT91.
    KOiK02358.
    OMAiSSYSISH.
    PhylomeDBiQ9ZT91.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    HAMAPiMF_00118_B. EF_Tu_B.
    InterProiIPR000795. EF_GTP-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR009000. Transl_B-barrel.
    IPR009001. Transl_elong_EF1A/Init_IF2_C.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    IPR004541. Transl_elong_EFTu/EF1A_bac/org.
    IPR004160. Transl_elong_EFTu/EF1A_C.
    [Graphical view]
    PfamiPF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF03143. GTP_EFTU_D3. 1 hit.
    [Graphical view]
    PRINTSiPR00315. ELONGATNFCT.
    SUPFAMiSSF50447. SSF50447. 1 hit.
    SSF50465. SSF50465. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEiPS00301. G_TR_1. 1 hit.
    PS51722. G_TR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9ZT91-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASVVLRNPS SKRLVPFSSQ IYSRCGASVT SSYSISHSIG GDDLSSSTFG    50
    TSSFWRSMAT FTRNKPHVNV GTIGHVDHGK TTLTAAITKV LAEEGKAKAI 100
    AFDEIDKAPE EKKRGITIAT AHVEYETAKR HYAHVDCPGH ADYVKNMITG 150
    AAQMDGGILV VSGPDGPMPQ TKEHILLARQ VGVPSLVCFL NKVDVVDDPE 200
    LLELVEMELR ELLSFYKFPG DDIPIIRGSA LSALQGTNDE IGRQAILKLM 250
    DAVDEYIPDP VRVLDKPFLM PIEDVFSIQG RGTVATGRIE QGVIKVGEEV 300
    EILGLREGGV PLKSTVTGVE MFKKILDNGQ AGDNVGLLLR GLKREDIQRG 350
    MVIAKPGSCK TYKKFEAEIY VLTKDEGGRH TAFFSNYRPQ FYLRTADITG 400
    KVELPENVKM VMPGDNVTAV FELIMPVPLE TGQRFALREG GRTVGAGVVS 450
    KVMT 454
    Length:454
    Mass (Da):49,410
    Last modified:May 1, 1999 - v1
    Checksum:iB7C2CA1DCC432FDA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1 – 1010MASVVLRNPS → MEEPVDREDIDLRVSSTDIG WNEFAAA in CAA61511. (PubMed:8555448)Curated
    Sequence conflicti310 – 3123VPL → CST in CAA61511. (PubMed:8555448)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X89227 mRNA. Translation: CAA61511.1.
    AC004044 Genomic DNA. Translation: AAD15337.1.
    AF069442 Genomic DNA. Translation: AAC79113.1.
    AL161495 Genomic DNA. Translation: CAB77778.1.
    CP002687 Genomic DNA. Translation: AEE82252.1.
    AY136421 mRNA. Translation: AAM97087.1.
    BT008755 mRNA. Translation: AAP49517.1.
    F14375 mRNA. Translation: CAA23078.1.
    PIRiT01400.
    RefSeqiNP_192202.1. NM_116527.3.
    UniGeneiAt.22275.

    Genome annotation databases

    EnsemblPlantsiAT4G02930.1; AT4G02930.1; AT4G02930.
    GeneIDi828134.
    KEGGiath:AT4G02930.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X89227 mRNA. Translation: CAA61511.1 .
    AC004044 Genomic DNA. Translation: AAD15337.1 .
    AF069442 Genomic DNA. Translation: AAC79113.1 .
    AL161495 Genomic DNA. Translation: CAB77778.1 .
    CP002687 Genomic DNA. Translation: AEE82252.1 .
    AY136421 mRNA. Translation: AAM97087.1 .
    BT008755 mRNA. Translation: AAP49517.1 .
    F14375 mRNA. Translation: CAA23078.1 .
    PIRi T01400.
    RefSeqi NP_192202.1. NM_116527.3.
    UniGenei At.22275.

    3D structure databases

    ProteinModelPortali Q9ZT91.
    SMRi Q9ZT91. Positions 65-453.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 13423. 2 interactions.
    STRINGi 3702.AT4G02930.1-P.

    Proteomic databases

    PaxDbi Q9ZT91.
    PRIDEi Q9ZT91.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G02930.1 ; AT4G02930.1 ; AT4G02930 .
    GeneIDi 828134.
    KEGGi ath:AT4G02930.

    Organism-specific databases

    GeneFarmi 4096. 415.
    TAIRi AT4G02930.

    Phylogenomic databases

    eggNOGi COG0050.
    HOGENOMi HOG000229290.
    InParanoidi Q9ZT91.
    KOi K02358.
    OMAi SSYSISH.
    PhylomeDBi Q9ZT91.

    Gene expression databases

    ArrayExpressi Q9ZT91.
    Genevestigatori Q9ZT91.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    HAMAPi MF_00118_B. EF_Tu_B.
    InterProi IPR000795. EF_GTP-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR009000. Transl_B-barrel.
    IPR009001. Transl_elong_EF1A/Init_IF2_C.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    IPR004541. Transl_elong_EFTu/EF1A_bac/org.
    IPR004160. Transl_elong_EFTu/EF1A_C.
    [Graphical view ]
    Pfami PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF03143. GTP_EFTU_D3. 1 hit.
    [Graphical view ]
    PRINTSi PR00315. ELONGATNFCT.
    SUPFAMi SSF50447. SSF50447. 1 hit.
    SSF50465. SSF50465. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00485. EF-Tu. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEi PS00301. G_TR_1. 1 hit.
    PS51722. G_TR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation, expression, and evolution of the gene encoding mitochondrial elongation factor Tu in Arabidopsis thaliana."
      Kuhlman P., Palmer J.D.
      Plant Mol. Biol. 29:1057-1070(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 342-454.
      Strain: cv. Columbia.
    6. "Proteomic approach to identify novel mitochondrial proteins in Arabidopsis."
      Kruft V., Eubel H., Jaensch L., Werhahn W., Braun H.-P.
      Plant Physiol. 127:1694-1710(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 296-306, SUBCELLULAR LOCATION.
      Tissue: Leaf and Stem.
    7. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
      Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
      Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: cv. Landsberg erecta.

    Entry informationi

    Entry nameiEFTM_ARATH
    AccessioniPrimary (citable) accession number: Q9ZT91
    Secondary accession number(s): Q39206
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 15, 2002
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3