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Protein

Elongation factor Tu, mitochondrial

Gene

TUFA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi74 – 81GTPBy similarity8
Nucleotide bindingi136 – 140GTPBy similarity5
Nucleotide bindingi191 – 194GTPBy similarity4

GO - Molecular functioni

  • ATP binding Source: TAIR
  • cobalt ion binding Source: TAIR
  • GTPase activity Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • translation elongation factor activity Source: UniProtKB-KW
  • zinc ion binding Source: TAIR

GO - Biological processi

  • response to cadmium ion Source: TAIR

Keywordsi

Molecular functionElongation factor
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor Tu, mitochondrial
Gene namesi
Name:TUFA
Ordered Locus Names:At4g02930
ORF Names:T4I9.19, T5J8.25
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G02930
TAIRilocus:2139325 AT4G02930

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 51MitochondrionSequence analysisAdd BLAST51
ChainiPRO_000000746652 – 454Elongation factor Tu, mitochondrialAdd BLAST403

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei82PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9ZT91
PRIDEiQ9ZT91

Expressioni

Gene expression databases

ExpressionAtlasiQ9ZT91 baseline and differential
GenevisibleiQ9ZT91 AT

Interactioni

Protein-protein interaction databases

BioGridi13423, 6 interactors
STRINGi3702.AT4G02930.1

Structurei

3D structure databases

ProteinModelPortaliQ9ZT91
SMRiQ9ZT91
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini65 – 261tr-type GAdd BLAST197

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni74 – 81G1By similarity8
Regioni115 – 119G2By similarity5
Regioni136 – 139G3By similarity4
Regioni191 – 194G4By similarity4
Regioni229 – 231G5By similarity3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0460 Eukaryota
COG0050 LUCA
HOGENOMiHOG000229290
KOiK02358
OMAiYGHIDCP
OrthoDBiEOG09360B5R
PhylomeDBiQ9ZT91

Family and domain databases

CDDicd03697 EFTU_II, 1 hit
HAMAPiMF_00118_B EF_Tu_B, 1 hit
InterProiView protein in InterPro
IPR004161 EFTu-like_2
IPR033720 EFTU_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
IPR004541 Transl_elong_EFTu/EF1A_bac/org
IPR004160 Transl_elong_EFTu/EF1A_C
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF03143 GTP_EFTU_D3, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00485 EF-Tu, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ZT91-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASVVLRNPS SKRLVPFSSQ IYSRCGASVT SSYSISHSIG GDDLSSSTFG
60 70 80 90 100
TSSFWRSMAT FTRNKPHVNV GTIGHVDHGK TTLTAAITKV LAEEGKAKAI
110 120 130 140 150
AFDEIDKAPE EKKRGITIAT AHVEYETAKR HYAHVDCPGH ADYVKNMITG
160 170 180 190 200
AAQMDGGILV VSGPDGPMPQ TKEHILLARQ VGVPSLVCFL NKVDVVDDPE
210 220 230 240 250
LLELVEMELR ELLSFYKFPG DDIPIIRGSA LSALQGTNDE IGRQAILKLM
260 270 280 290 300
DAVDEYIPDP VRVLDKPFLM PIEDVFSIQG RGTVATGRIE QGVIKVGEEV
310 320 330 340 350
EILGLREGGV PLKSTVTGVE MFKKILDNGQ AGDNVGLLLR GLKREDIQRG
360 370 380 390 400
MVIAKPGSCK TYKKFEAEIY VLTKDEGGRH TAFFSNYRPQ FYLRTADITG
410 420 430 440 450
KVELPENVKM VMPGDNVTAV FELIMPVPLE TGQRFALREG GRTVGAGVVS

KVMT
Length:454
Mass (Da):49,410
Last modified:May 1, 1999 - v1
Checksum:iB7C2CA1DCC432FDA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 10MASVVLRNPS → MEEPVDREDIDLRVSSTDIG WNEFAAA in CAA61511 (PubMed:8555448).Curated10
Sequence conflicti310 – 312VPL → CST in CAA61511 (PubMed:8555448).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89227 mRNA Translation: CAA61511.1
AC004044 Genomic DNA Translation: AAD15337.1
AF069442 Genomic DNA Translation: AAC79113.1
AL161495 Genomic DNA Translation: CAB77778.1
CP002687 Genomic DNA Translation: AEE82252.1
AY136421 mRNA Translation: AAM97087.1
BT008755 mRNA Translation: AAP49517.1
F14375 mRNA Translation: CAA23078.1
PIRiT01400
RefSeqiNP_192202.1, NM_116527.4
UniGeneiAt.22275

Genome annotation databases

EnsemblPlantsiAT4G02930.1; AT4G02930.1; AT4G02930
GeneIDi828134
GrameneiAT4G02930.1; AT4G02930.1; AT4G02930
KEGGiath:AT4G02930

Similar proteinsi

Entry informationi

Entry nameiEFTM_ARATH
AccessioniPrimary (citable) accession number: Q9ZT91
Secondary accession number(s): Q39206
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: May 1, 1999
Last modified: April 25, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health