Reviewed, UniProtKB/Swiss-Prot Q9ZT84 (G3OX2_ARATH)

Names and origin

Protein names

Recommended name:
    Gibberellin 3-beta-dioxygenase 2
    EC=1.14.11.15
Alternative name(s):
    Gibberellin 3 beta-hydroxylase 2
    GA 3-oxidase 2
      Short name=AtGA3ox2

Gene names

Name:	GA4H
Ordered Locus Names:	At1g80340
ORF Names:	F5I6.9

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Converts the inactive gibbberellin precursors GA9 and GA20 in the bioactives gibberellins GA4 and GA1.
Catalytic activity	Gibberellin 20 + 2-oxoglutarate + O(2) = gibberellin 1 + succinate + CO(2).
Cofactor	Ascorbate By similarity. Iron By similarity.
Tissue specificity	Found in the cortex and the endodermis of the embryo axis in germinating seeds.
Developmental stage	Expressed in germinating seeds and in very young seedlings.
Induction	Not under feedback regulation. Regulated by phytochrome. Induced by red light pulse and reaches its maximum level after 12 hours. Transcriptionally regulated by LEAFY COTYLEDON2 and FUSCA3.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family. GA3OX subfamily.
Biophysicochemical properties	Kinetic parameters: KM=1.0 µM for GA9 KM=13 µM for GA20

Ontologies

Keywords
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	gibberellin 3-beta-dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Gibberellin 3-beta-dioxygenase 2		PRO_0000067314
Sites
	Active site	292	1	Potential
	Metal binding	225	1	Iron By similarity
	Metal binding	227	1	Iron By similarity
	Metal binding	282	1	Iron By similarity
Experimental info
	Sequence conflict	204	1	V → A in AAC83647. Ref.1
	Sequence conflict	305	1	I → V in AAC83647. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9ZT84-1 [UniParc]. Last modified December 6, 2005. Version 2. Checksum: DC5846E65CD44FAB	FASTA	347	38,782
	10 20 30 40 50 60 MSSTLSDVFR SHPIHIPLSN PPDFKSLPDS YTWTPKDDLL FSASASDETL PLIDLSDIHV 70 80 90 100 110 120 ATLVGHACTT WGAFQITNHG VPSRLLDDIE FLTGSLFRLP VQRKLKAARS ENGVSGYGVA 130 140 150 160 170 180 RIASFFNKKM WSEGFTVIGS PLHDFRKLWP SHHLKYCEII EEYEEHMQKL AAKLMWFALG 190 200 210 220 230 240 SLGVEEKDIQ WAGPNSDFQG TQAVIQLNHY PKCPEPDRAM GLAAHTDSTL MTILYQNNTA 250 260 270 280 290 300 GLQVFRDDVG WVTAPPVPGS LVVNVGDLLH ILTNGIFPSV LHRARVNHVR SRFSMAYLWG 310 320 330 340 PPSDIMISPL PKLVDPLQSP LYPSLTWKQY LATKATHFNQ SLSIIRN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Phytochrome regulation and differential expression of gibberellin 3beta-hydroxylase genes in germinating Arabidopsis seeds." Yamaguchi S., Smith M.W., Brown R.G.S., Kamiya Y., Sun T.-P. Plant Cell 10:2115-2126(1998) [PubMed: 9836749] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, BIOPHYSICOCHEMICAL PROPERTIES, DEVELOPMENTAL STAGE.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Distinct cell-specific expression patterns of early and late gibberellin biosynthetic genes during Arabidopsis seed germination." Yamaguchi S., Kamiya Y., Sun T.-P. Plant J. 28:443-453(2001) [PubMed: 11737781] [Abstract] Cited for: TISSUE SPECIFICITY.
[4]	"AtGA3ox2, a key gene responsible for bioactive gibberellin biosynthesis, is regulated during embryogenesis by LEAFY COTYLEDON2 and FUSCA3 in Arabidopsis." Curaba J., Moritz T., Blervaque R., Parcy F., Raz V., Herzog M., Vachon G. Plant Physiol. 136:3660-3669(2004) [PubMed: 15516508] [Abstract] Cited for: CHARACTERIZATION, REGULATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF070937 mRNA. Translation: AAC83647.1. AC018848 Genomic DNA. Translation: AAG52442.1.
PIR	A96835. T51691.
RefSeq	NP_178150.1.
UniGene	At.10925
3D structure databases
HSSP	HSSP built from PDB template 1GP4 based on UniProtKB Q96323.
ModBase	Search...
Genome annotation databases
GeneID	844374.
GenomeReviews	Gene locus AT1G80340 in contig CT485782_GR.
KEGG	ath:AT1G80340.
NMPDR	fig|3702.1.peg.7621.
Organism-specific databases
GeneFarm	5168. 494.
TAIR	At1g80340.
Enzyme and pathway databases
BioCyc	MetaCyc:AT1G80340-MON.
Gene expression databases
GermOnline	AT1G80340. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR005123. 2OG-FeII_Oase. IPR002283. Isopenicillin-N_synthase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
PRINTS	PR00682. IPNSYNTHASE.
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

G3OX2_ARATH

Accession

Primary (citable) accession number: Q9ZT84
Secondary accession number(s): Q9C970

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	November 4, 2008
This is version 51 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)