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Protein

Metal tolerance protein 1

Gene

MTP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates zinc accumulation in roots and confers resistance to zinc. Involved in sequestration of excess zinc in the cytoplasm into vacuoles to maintain zinc homeostasis. Can also transport cadmium with a low efficiency.4 Publications

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • metal ion transmembrane transporter activity Source: TAIR
  • zinc ion transmembrane transporter activity Source: TAIR

GO - Biological processi

  • cellular zinc ion homeostasis Source: TAIR
  • regulation of sequestering of zinc ion Source: GO_Central
  • response to zinc ion Source: GO_Central
  • zinc II ion transport Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Ion transport, Transport, Zinc transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-264876. Insulin processing.
R-ATH-435368. Zinc efflux and compartmentalization by the SLC30 family.

Protein family/group databases

TCDBi2.A.4.3.4. the cation diffusion facilitator (cdf) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Metal tolerance protein 1
Short name:
AtMTP1
Alternative name(s):
ZAT1p
Zinc transporter ZAT-1
Cleaved into the following chain:
Gene namesi
Name:MTP1
Synonyms:ZAT, ZAT1
Ordered Locus Names:At2g46800
ORF Names:F19D11.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G46800.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5656CytoplasmicSequence analysisAdd
BLAST
Transmembranei57 – 7721HelicalSequence analysisAdd
BLAST
Topological domaini78 – 8912VacuolarSequence analysisAdd
BLAST
Transmembranei90 – 11021HelicalSequence analysisAdd
BLAST
Topological domaini111 – 12212CytoplasmicSequence analysisAdd
BLAST
Transmembranei123 – 14321HelicalSequence analysisAdd
BLAST
Topological domaini144 – 15916VacuolarSequence analysisAdd
BLAST
Transmembranei160 – 18021HelicalSequence analysisAdd
BLAST
Topological domaini181 – 26383CytoplasmicSequence analysisAdd
BLAST
Transmembranei264 – 28421HelicalSequence analysisAdd
BLAST
Topological domaini285 – 2906VacuolarSequence analysis
Transmembranei291 – 31121HelicalSequence analysisAdd
BLAST
Topological domaini312 – 39887CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: TAIR
  • vacuolar membrane Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth condition, but hypersensitivity to elevated levels of zinc.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 398398Metal tolerance protein 1PRO_0000032665Add
BLAST
Chaini26 – 398373Metal tolerance protein 1 short formCuratedPRO_0000032666Add
BLAST

Proteomic databases

PaxDbiQ9ZT63.
PRIDEiQ9ZT63.

Expressioni

Tissue specificityi

Ubiquitously expressed at low levels.1 Publication

Inductioni

By treatment with high concentrations of zinc.1 Publication

Gene expression databases

ExpressionAtlasiQ9ZT63. baseline and differential.
GenevisibleiQ9ZT63. AT.

Interactioni

Protein-protein interaction databases

BioGridi4628. 13 interactions.
MINTiMINT-8069178.
STRINGi3702.AT2G46800.1.

Structurei

3D structure databases

ProteinModelPortaliQ9ZT63.
SMRiQ9ZT63. Positions 58-155.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni182 – 23251Required for zinc-bindingAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi182 – 23251His-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1482. Eukaryota.
COG1230. LUCA.
HOGENOMiHOG000079023.
InParanoidiQ9ZT63.
KOiK14689.
OMAiQECVATH.
PhylomeDBiQ9ZT63.

Family and domain databases

Gene3Di1.20.1510.10. 2 hits.
InterProiIPR002524. Cation_efflux.
IPR027469. Cation_efflux_TMD.
[Graphical view]
PANTHERiPTHR11562. PTHR11562. 2 hits.
PfamiPF01545. Cation_efflux. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01297. CDF. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ZT63-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESSSPHHSH IVEVNVGKSD EERIIVASKV CGEAPCGFSD SKNASGDAHE
60 70 80 90 100
RSASMRKLCI AVVLCLVFMS VEVVGGIKAN SLAILTDAAH LLSDVAAFAI
110 120 130 140 150
SLFSLWAAGW EATPRQTYGF FRIEILGALV SIQLIWLLTG ILVYEAIIRI
160 170 180 190 200
VTETSEVNGF LMFLVAAFGL VVNIIMAVLL GHDHGHSHGH GHGHGHDHHN
210 220 230 240 250
HSHGVTVTTH HHHHDHEHGH SHGHGEDKHH AHGDVTEQLL DKSKTQVAAK
260 270 280 290 300
EKRKRNINLQ GAYLHVLGDS IQSVGVMIGG AIIWYNPEWK IVDLICTLAF
310 320 330 340 350
SVIVLGTTIN MIRNILEVLM ESTPREIDAT KLEKGLLEME EVVAVHELHI
360 370 380 390
WAITVGKVLL ACHVNIRPEA DADMVLNKVI DYIRREYNIS HVTIQIER
Length:398
Mass (Da):43,827
Last modified:July 19, 2005 - v2
Checksum:i7E20E0B29237BB23
GO

Sequence cautioni

The sequence AAD11757.1 differs from that shown. Reason: Frameshift at positions 186 and 194. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF072858 mRNA. Translation: AAD11757.1. Frameshift.
AC005310 Genomic DNA. Translation: AAC33498.1.
CP002685 Genomic DNA. Translation: AEC10755.1.
CP002685 Genomic DNA. Translation: AEC10756.1.
AY086033 mRNA. Translation: AAM63243.1.
PIRiT02681.
RefSeqiNP_182203.1. NM_130246.2.
NP_850459.1. NM_180128.2.
UniGeneiAt.12961.

Genome annotation databases

EnsemblPlantsiAT2G46800.1; AT2G46800.1; AT2G46800.
AT2G46800.2; AT2G46800.2; AT2G46800.
GeneIDi819293.
GrameneiAT2G46800.1; AT2G46800.1; AT2G46800.
AT2G46800.2; AT2G46800.2; AT2G46800.
KEGGiath:AT2G46800.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF072858 mRNA. Translation: AAD11757.1. Frameshift.
AC005310 Genomic DNA. Translation: AAC33498.1.
CP002685 Genomic DNA. Translation: AEC10755.1.
CP002685 Genomic DNA. Translation: AEC10756.1.
AY086033 mRNA. Translation: AAM63243.1.
PIRiT02681.
RefSeqiNP_182203.1. NM_130246.2.
NP_850459.1. NM_180128.2.
UniGeneiAt.12961.

3D structure databases

ProteinModelPortaliQ9ZT63.
SMRiQ9ZT63. Positions 58-155.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4628. 13 interactions.
MINTiMINT-8069178.
STRINGi3702.AT2G46800.1.

Protein family/group databases

TCDBi2.A.4.3.4. the cation diffusion facilitator (cdf) family.

Proteomic databases

PaxDbiQ9ZT63.
PRIDEiQ9ZT63.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G46800.1; AT2G46800.1; AT2G46800.
AT2G46800.2; AT2G46800.2; AT2G46800.
GeneIDi819293.
GrameneiAT2G46800.1; AT2G46800.1; AT2G46800.
AT2G46800.2; AT2G46800.2; AT2G46800.
KEGGiath:AT2G46800.

Organism-specific databases

TAIRiAT2G46800.

Phylogenomic databases

eggNOGiKOG1482. Eukaryota.
COG1230. LUCA.
HOGENOMiHOG000079023.
InParanoidiQ9ZT63.
KOiK14689.
OMAiQECVATH.
PhylomeDBiQ9ZT63.

Enzyme and pathway databases

ReactomeiR-ATH-264876. Insulin processing.
R-ATH-435368. Zinc efflux and compartmentalization by the SLC30 family.

Miscellaneous databases

PROiQ9ZT63.

Gene expression databases

ExpressionAtlasiQ9ZT63. baseline and differential.
GenevisibleiQ9ZT63. AT.

Family and domain databases

Gene3Di1.20.1510.10. 2 hits.
InterProiIPR002524. Cation_efflux.
IPR027469. Cation_efflux_TMD.
[Graphical view]
PANTHERiPTHR11562. PTHR11562. 2 hits.
PfamiPF01545. Cation_efflux. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01297. CDF. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Overexpression of a novel Arabidopsis gene related to putative zinc-transporter genes from animals can lead to enhanced zinc resistance and accumulation."
    Van der Zaal B.J., Neuteboom L.W., Pinas J.E., Chardonnens A.N., Schat H., Verkleij J.A.C., Hooykaas P.J.J.
    Plant Physiol. 119:1047-1055(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Strain: cv. C24.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Characterization of the ZAT1p zinc transporter from Arabidopsis thaliana in microbial model organisms and reconstituted proteoliposomes."
    Bloss T., Clemens S., Nies D.H.
    Planta 214:783-791(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF N-TERMINUS, FUNCTION.
  6. Cited for: GENE FAMILY, NOMENCLATURE.
  7. "Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana."
    Shimaoka T., Ohnishi M., Sazuka T., Mitsuhashi N., Hara-Nishimura I., Shimazaki K., Maeshima M., Yokota A., Tomizawa K., Mimura T.
    Plant Cell Physiol. 45:672-683(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. "Zinc transporter of Arabidopsis thaliana AtMTP1 is localized to vacuolar membranes and implicated in zinc homeostasis."
    Kobae Y., Uemura T., Sato M.H., Ohnishi M., Mimura T., Nakagawa T., Maeshima M.
    Plant Cell Physiol. 45:1749-1758(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, NOMENCLATURE.
  9. "MTP1 mops up excess zinc in Arabidopsis cells."
    Kraemer U.
    Trends Plant Sci. 10:313-315(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  10. "A mutant strain Arabidopsis thaliana that lacks vacuolar membrane zinc transporter MTP1 revealed the latent tolerance to excessive zinc."
    Kawachi M., Kobae Y., Mori H., Tomioka R., Lee Y., Maeshima M.
    Plant Cell Physiol. 50:1156-1170(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION BY ZINC, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiMTP1_ARATH
AccessioniPrimary (citable) accession number: Q9ZT63
Secondary accession number(s): O81036
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: July 6, 2016
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.