Q9ZSV1 (PARP1_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Poly [ADP-ribose] polymerase 1
Short name=PARP-1
EC=2.4.2.30

Alternative name(s):
NAD(+) ADP-ribosyltransferase 1
Short name=ADPRT-1
Poly[ADP-ribose] synthase 1

Gene names

Name:

PARP1

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	980 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks By similarity.
Catalytic activity	NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.
Subcellular location	Nucleus Potential.
Sequence similarities	Contains 1 BRCT domain. Contains 1 PARP alpha-helical domain. Contains 1 PARP catalytic domain. Contains 2 PARP-type zinc fingers. Contains 1 SAP domain.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding NAD Zinc
Molecular function	Glycosyltransferase Transferase
PTM	ADP-ribosylation
Gene Ontology (GO)
Biological process	protein ADP-ribosylation Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW NAD binding Inferred from electronic annotation. Source: InterPro NAD+ ADP-ribosyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 980	980	Poly [ADP-ribose] polymerase 1		PRO_0000260500
Regions
	Domain	257 – 291	35	SAP
	Domain	385 – 476	92	BRCT
	Domain	629 – 748	120	PARP alpha-helical
	Domain	755 – 980	226	PARP catalytic
	Zinc finger	8 – 91	84	PARP-type 1
	Zinc finger	104 – 184	81	PARP-type 2
Experimental info
	Sequence conflict	54 – 64	11	Missing in CAA10889. Ref.1
	Sequence conflict	292	1	L → I in CAA10889. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9ZSV1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 9D8AED26BC37E5C1	FASTA	980	110,475
	10 20 30 40 50 60 MAAPPKAWKA EYAKSGRASC KSCRSPIAKD QLRLGKMVQA SQFDGFMPMW NHARCIFSKK 70 80 90 100 110 120 NQIKSVDDVE GIDALRWDDQ EKIRNYVGSA SAGTSSTAAP PEKCTIEIAP SARTSCRRCS 130 140 150 160 170 180 EKITKGSVRL SAKLESEGPK GIPWYHANCF FEVSPSATVE KFSGWDTLSD EDKRTMLDLV 190 200 210 220 230 240 KKDVGNNEQN KGSKRKKSEN DIDSYKSARL DESTSEGTVR NKGQLVDPRG SNTSSADIQL 250 260 270 280 290 300 KLKEQSDTLW KLKDGLKTHV SAAELRDMLE ANGQDTSGPE RHLLDRCADG MLFGALGPCP 310 320 330 340 350 360 VCANGMYYYN GQYQCSGNVS EWSKCTYSAT EPVRVKKKWQ IPHGTKNDYL MKWFKSQKVK 370 380 390 400 410 420 KPERVLPPMS PEKSGSKATQ RTSLLSSKGL DKLRFSVVGQ SKEAANEWIE KLKLAGANFY 430 440 450 460 470 480 ARVVKDIDCL IACGELDNEN AEVRKARRLK IPIVREGYIG ECVKKNKMLP FDLYKLENAL 490 500 510 520 530 540 ESSKGSTVTV KVKGRSAVHE SSGLQDTAHI LEDGKSIYNA TLNMSDLALG VNSYYVLQII 550 560 570 580 590 600 EQDDGSECYV FRKWGRVGSE KIGGQKLEEM SKTEAIKEFK RLFLEKTGNS WEAWECKTNF 610 620 630 640 650 660 RKQPGRFYPL DVDYGVKKAP KRKDISEMKS SLAPQLLELM KMLFNVETYR AAMMEFEINM 670 680 690 700 710 720 SEMPLGKLSK ENIEKGFEAL TEIQNLLKDT ADQALAVRES LIVAASNRFF TLIPSIHPHI 730 740 750 760 770 780 IRDEDDLMIK AKMLEALQDI EIASKIVGFD SDSDESLDDK YMKLHCDITP LAHDSEDYKL 790 800 810 820 830 840 IEQYLLNTHA PTHKDWSLEL EEVFSLDRDG ELNKYSRYKN NLHNKMLLWH GSRLTNFVGI 850 860 870 880 890 900 LSQGLRIAPP EAPVTGYMFG KGLYFADLVS KSAQYCYVDR NNPVGLMLLS EVALGDMYEL 910 920 930 940 950 960 KKATSMDKPP RGKHSTKGLG KTVPLESEFV KWRDDVVVPC GKPVPSSIRS SELMYNEYIV 970 980 YNTSQVKMQF LLKVRFHHKR

References

[1]	"Higher plants possess two structurally different poly(ADP-ribose) polymerases." Babiychuk E., Cottrill P.B., Storozhenko S., Fuangthong M., Chen Y., O'Farrell M.K., Van Montagu M., Inze D., Kushnir S. Plant J. 15:635-645(1998) [PubMed: 9778846] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Purification and cDNA cloning of maize poly(ADP)-ribose polymerase." Mahajan P.B., Zuo Z. Plant Physiol. 118:895-905(1998) [PubMed: 9808734] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ222589 mRNA. Translation: CAA10889.1. AF093627 mRNA. Translation: AAC79704.1.
PIR	T03657.
3D structure databases
HSSP	HSSP built from PDB template 1GS0 based on UniProtKB O88554.
ProteinModelPortal	Q9ZSV1.
SMR	Q9ZSV1. Positions 6-91.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	GRMZM5G831712_T02; GRMZM5G831712_P02; GRMZM5G831712.
Organism-specific databases
Gramene	Q9ZSV1.
MaizeGDB	403204.
Phylogenomic databases
GeneTree	EPGT00070000030126.
Family and domain databases
InterPro	IPR001357. BRCT. IPR008288. NAD_ADPRT. IPR012982. PADR1. IPR012317. Poly(ADP-ribose)pol_cat_dom. IPR004102. Poly(ADP-ribose)pol_reg_dom. IPR003034. SAP_DNA-bd. IPR008893. WGR_domain. IPR001510. Znf_PARP. [Graphical view]
Gene3D	G3DSA:1.20.142.10. PARP_reg. 1 hit. G3DSA:3.30.1740.10. Znf_PARP. 2 hits.
Pfam	PF00533. BRCT. 1 hit. PF08063. PADR1. 1 hit. PF00644. PARP. 1 hit. PF02877. PARP_reg. 1 hit. PF05406. WGR. 1 hit. PF00645. zf-PARP. 2 hits. [Graphical view]
PIRSF	PIRSF000489. NAD_ADPRT. 1 hit.
SMART	SM00292. BRCT. 1 hit. SM00773. WGR. 1 hit. [Graphical view]
SUPFAM	SSF52113. BRCT. 1 hit. SSF47587. PARP_reg. 1 hit. SSF142921. SSF142921. 1 hit.
PROSITE	PS50172. BRCT. 1 hit. PS51060. PARP_ALPHA_HD. 1 hit. PS51059. PARP_CATALYTIC. 1 hit. PS50064. PARP_ZN_FINGER_2. 2 hits. PS50800. SAP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PARP1_MAIZE

Accession

Primary (citable) accession number: Q9ZSV1
Secondary accession number(s): O24570

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	May 1, 1999
Last modified:	September 21, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program