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Protein

Threonine dehydratase biosynthetic, chloroplastic

Gene

OMR1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia.

Catalytic activityi

L-threonine = 2-oxobutanoate + NH3.

Cofactori

Enzyme regulationi

Allosterically inhibited by isoleucine. Strain GM11b is isoleucine feedback insensitive and is resistant to the antimetabolite L-O-methylthreonine.

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 2-oxobutanoate from L-threonine.
Proteins known to be involved in this subpathway in this organism are:
  1. Threonine dehydratase (AXX17_At3g09750), Threonine dehydratase biosynthetic, chloroplastic (OMR1), Threonine dehydratase (At3g10050)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-oxobutanoate from L-threonine, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • L-threonine ammonia-lyase activity Source: TAIR
  • pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  • isoleucine biosynthetic process Source: TAIR

Keywordsi

Molecular functionAllosteric enzyme, Lyase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis, Isoleucine biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciARA:AT3G10050-MONOMER
SABIO-RKQ9ZSS6
UniPathwayiUPA00047; UER00054

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine dehydratase biosynthetic, chloroplastic (EC:4.3.1.19)
Alternative name(s):
Threonine deaminase
Short name:
TD
Gene namesi
Name:OMR1
Ordered Locus Names:At3g10050
ORF Names:T22K18.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G10050
TAIRilocus:2100078 AT3G10050

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi499R → C in omr1; loss of Ile feedback sensitivity; when associated with H-544. 1
Mutagenesisi544R → H in omr1; loss of Ile feedback sensitivity; when associated with C-499. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 91ChloroplastSequence analysisAdd BLAST91
ChainiPRO_000003361292 – 592Threonine dehydratase biosynthetic, chloroplasticAdd BLAST501

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei141N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiQ9ZSS6

Expressioni

Gene expression databases

ExpressionAtlasiQ9ZSS6 baseline and differential
GenevisibleiQ9ZSS6 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G10050.1

Structurei

3D structure databases

ProteinModelPortaliQ9ZSS6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini419 – 490ACT-like 1PROSITE-ProRule annotationAdd BLAST72
Domaini512 – 583ACT-like 2PROSITE-ProRule annotationAdd BLAST72

Sequence similaritiesi

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG1250 Eukaryota
COG1171 LUCA
HOGENOMiHOG000046975
InParanoidiQ9ZSS6
KOiK01754
OMAiRNHGAAY
OrthoDBiEOG093607Z6
PhylomeDBiQ9ZSS6

Family and domain databases

Gene3Di3.40.1020.10, 1 hit
InterProiView protein in InterPro
IPR001926 PLP-dep
IPR000634 Ser/Thr_deHydtase_PyrdxlP-BS
IPR001721 TD_ACT-like
IPR038110 TD_ACT-like_sf
IPR005787 Thr_deHydtase_biosynth
IPR036052 Trypto_synt_PLP_dependent
PfamiView protein in Pfam
PF00291 PALP, 1 hit
PF00585 Thr_dehydrat_C, 2 hits
SUPFAMiSSF53686 SSF53686, 1 hit
TIGRFAMsiTIGR01124 ilvA_2Cterm, 1 hit
PROSITEiView protein in PROSITE
PS51672 ACT_LIKE, 2 hits
PS00165 DEHYDRATASE_SER_THR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ZSS6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSVQLPTAQ SSLRSHIHRP SKPVVGFTHF SSRSRIAVAV LSRDETSMTP
60 70 80 90 100
PPPKLPLPRL KVSPNSLQYP AGYLGAVPER TNEAENGSIA EAMEYLTNIL
110 120 130 140 150
STKVYDIAIE SPLQLAKKLS KRLGVRMYLK REDLQPVFSF KLRGAYNMMV
160 170 180 190 200
KLPADQLAKG VICSSAGNHA QGVALSASKL GCTAVIVMPV TTPEIKWQAV
210 220 230 240 250
ENLGATVVLF GDSYDQAQAH AKIRAEEEGL TFIPPFDHPD VIAGQGTVGM
260 270 280 290 300
EITRQAKGPL HAIFVPVGGG GLIAGIAAYV KRVSPEVKII GVEPADANAM
310 320 330 340 350
ALSLHHGERV ILDQVGGFAD GVAVKEVGEE TFRISRNLMD GVVLVTRDAI
360 370 380 390 400
CASIKDMFEE KRNILEPAGA LALAGAEAYC KYYGLKDVNV VAITSGANMN
410 420 430 440 450
FDKLRIVTEL ANVGRQQEAV LATLMPEKPG SFKQFCELVG PMNISEFKYR
460 470 480 490 500
CSSEKEAVVL YSVGVHTAGE LKALQKRMES SQLKTVNLTT SDLVKDHLRY
510 520 530 540 550
LMGGRSTVGD EVLCRFTFPE RPGALMNFLD SFSPRWNITL FHYRGQGETG
560 570 580 590
ANVLVGIQVP EQEMEEFKNR AKALGYDYFL VSDDDYFKLL MH
Length:592
Mass (Da):64,635
Last modified:May 1, 1999 - v1
Checksum:i16658747052FAE7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF096281 mRNA Translation: AAC97936.1
AF221984 Genomic DNA Translation: AAF32370.1
AF177212 mRNA Translation: AAD54324.1
AC010927 Genomic DNA Translation: AAF04418.1
CP002686 Genomic DNA Translation: AEE74854.1
AY065037 mRNA Translation: AAL57674.1
PIRiT51712
RefSeqiNP_187616.1, NM_111840.3
UniGeneiAt.10929

Genome annotation databases

EnsemblPlantsiAT3G10050.1; AT3G10050.1; AT3G10050
GeneIDi820166
GrameneiAT3G10050.1; AT3G10050.1; AT3G10050
KEGGiath:AT3G10050

Similar proteinsi

Entry informationi

Entry nameiTHD1_ARATH
AccessioniPrimary (citable) accession number: Q9ZSS6
Secondary accession number(s): Q9SPF1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: May 1, 1999
Last modified: April 25, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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