Q9ZSM8 (EZA1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histone-lysine N-methyltransferase EZA1
EC=2.1.1.43

Alternative name(s):
CURLY LEAF-like 1
Protein SET DOMAIN GROUP 10

Gene names

Name:	EZA1
Synonyms:	SDG10, SET10
Ordered Locus Names:	At4g02020
ORF Names:	T10M13.3

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	856 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Polycomb group (PcG) protein. Catalytic subunit of some PcG multiprotein complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target genes. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development By similarity.
Catalytic activity	S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the histone-lysine methyltransferase family. EZ subfamily. Contains 1 SANT domain. Contains 1 SET domain.
Sequence caution	The sequence AAC78694.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB80695.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Repressor Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	endosperm development Inferred from genetic interaction. Source: TAIR regulation of gene expression by genetic imprinting Inferred from mutant phenotype. Source: TAIR regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from direct assay. Source: TAIR plasmodesma Inferred from direct assay. Source: TAIR
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro histone-lysine N-methyltransferase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: TAIR single-stranded RNA binding Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 856	856	Histone-lysine N-methyltransferase EZA1		PRO_0000213996
Regions
	Domain	489 – 539	51	SANT
	Domain	706 – 826	121	SET
	Compositional bias	17 – 33	17	Asp/Glu-rich (acidic)
	Compositional bias	605 – 680	76	Cys-rich

Sequences

	Sequence		Length	Mass (Da)
	Q9ZSM8 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: DD4B099C936F197C	FASTA	856	95,396
	10 20 30 40 50 60 MVTDDSNSSG RIKSHVDDDD DGEEEEDRLE GLENRLSELK RKIQGERVRS IKEKFEANRK 70 80 90 100 110 120 KVDAHVSPFS SAASSRATAE DNGNSNMLSS RMRMPLCKLN GFSHGVGDRD YVPTKDVISA 130 140 150 160 170 180 SVKLPIAERI PPYTTWIFLD RNQRMAEDQS VVGRRQIYYE QHGGETLICS DSEEEPEPEE 190 200 210 220 230 240 EKREFSEGED SIIWLIGQEY GMGEEVQDAL CQLLSVDASD ILERYNELKL KDKQNTEEFS 250 260 270 280 290 300 NSGFKLGISL EKGLGAALDS FDNLFCRRCL VFDCRLHGCS QPLISASEKQ PYWSDYEGDR 310 320 330 340 350 360 KPCSKHCYLQ LKAVREVPET CSNFASKAEE KASEEECSKA VSSDVPHAAA SGVSLQVEKT 370 380 390 400 410 420 DIGIKNVDSS SGVEQEHGIR GKREVPILKD SNDLPNLSNK KQKTAASDTK MSFVNSVPSL 430 440 450 460 470 480 DQALDSTKGD QGGTTDNKVN RDSEADAKEV GEPIPDNSVH DGGSSICQPH HGSGNGAIII 490 500 510 520 530 540 AEMSETSRPS TEWNPIEKDL YLKGVEIFGR NSCLIARNLL SGLKTCLDVS NYMRENEVSV 550 560 570 580 590 600 FRRSSTPNLL LDDGRTDPGN DNDEVPPRTR LFRRKGKTRK LKYSTKSAGH PSVWKRIAGG 610 620 630 640 650 660 KNQSCKQYTP CGCLSMCGKD CPCLTNETCC EKYCGCSKSC KNRFRGCHCA KSQCRSRQCP 670 680 690 700 710 720 CFAAGRECDP DVCRNCWVSC GDGSLGEAPR RGEGQCGNMR LLLRQQQRIL LGKSDVAGWG 730 740 750 760 770 780 AFLKNSVSKN EYLGEYTGEL ISHHEADKRG KIYDRANSSF LFDLNDQYVL DAQRKGDKLK 790 800 810 820 830 840 FANHSAKPNC YAKVMFVAGD HRVGIFANER IEASEELFYD YRYGPDQAPV WARKPEGSKK 850 DDSAITHRRA RKHQSH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"EZA1, a novel polycomb group gene from Arabidopsis thaliana." Bilodeau P., Luo M., Dennis E.S., Peacock W.J., Chaudhury A.M. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF100163 mRNA. Translation: AAD09108.1. AF001308 Genomic DNA. Translation: AAC78694.1. Sequence problems. AL161493 Genomic DNA. Translation: CAB80695.1. Sequence problems. CP002687 Genomic DNA. Translation: AEE82112.1. AY057477 mRNA. Translation: AAL09711.1. AY090293 mRNA. Translation: AAL90954.1.
IPI	IPI00543300.
PIR	T01503. T52415.
RefSeq	NP_567221.1. NM_116433.2.
UniGene	At.3858.
3D structure databases
ProteinModelPortal	Q9ZSM8.
SMR	Q9ZSM8. Positions 605-826.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-35029N.
IntAct	Q9ZSM8. 5 interactions.
STRING	Q9ZSM8.
Proteomic databases
PRIDE	Q9ZSM8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G02020.1; AT4G02020.1; AT4G02020.
GeneID	828165.
GenomeReviews	Gene locus AT4G02020 in contig CT486007_GR.
KEGG	ath:AT4G02020.
NMPDR	fig|3702.1.peg.18006.
Organism-specific databases
GeneFarm	2271.
TAIR	At4g02020.
Phylogenomic databases
GeneTree	EPGT00070000028476.
HOGENOM	HBG633142.
InParanoid	Q9ZSM8.
OMA	QSCKQYT.
PhylomeDB	Q9ZSM8.
ProtClustDB	CLSN2689281.
Gene expression databases
ArrayExpress	Q9ZSM8.
Genevestigator	Q9ZSM8.
GermOnline	AT4G02020. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001005. SANT_DNA-bd. IPR001214. SET_dom. [Graphical view]
KO	K11430.
Pfam	PF00856. SET. 1 hit. [Graphical view]
SMART	SM00717. SANT. 1 hit. SM00317. SET. 1 hit. [Graphical view]
PROSITE	PS51293. SANT. False negative. PS50280. SET. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EZA1_ARATH

Accession

Primary (citable) accession number: Q9ZSM8
Secondary accession number(s): O04246

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2003
Last sequence update:	May 1, 1999
Last modified:	December 14, 2011
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program