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Protein

Calcium-dependent protein kinase 22

Gene

CPK22

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei65ATPPROSITE-ProRule annotation1
Active sitei164Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi42 – 50ATPPROSITE-ProRule annotation9
Calcium bindingi359 – 3701PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi395 – 4062PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi431 – 4423PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi466 – 4774PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-3371571. HSF1-dependent transactivation.
R-ATH-442729. CREB phosphorylation through the activation of CaMKII.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase 22 (EC:2.7.11.1)
Gene namesi
Name:CPK22
Ordered Locus Names:At4g04710
ORF Names:T4B21.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G04710.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00003633442 – 498Calcium-dependent protein kinase 22Add BLAST497

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1
Modified residuei204PhosphoserineBy similarity1

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiQ9ZSA3.
PRIDEiQ9ZSA3.

PTM databases

SwissPalmiQ9ZSA3.

Expressioni

Gene expression databases

ExpressionAtlasiQ9ZSA3. baseline and differential.
GenevisibleiQ9ZSA3. AT.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi3702.AT4G04710.1.

Structurei

3D structure databases

ProteinModelPortaliQ9ZSA3.
SMRiQ9ZSA3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 305Protein kinasePROSITE-ProRule annotationAdd BLAST270
Domaini346 – 381EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini382 – 417EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini418 – 453EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini454 – 488EF-hand 4PROSITE-ProRule annotationAdd BLAST35

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni309 – 339Autoinhibitory domainBy similarityAdd BLAST31

Domaini

There are 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (309-339) inactivates kinase activity under calcium-free conditions (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
InParanoidiQ9ZSA3.
KOiK13412.
OrthoDBiEOG093609AJ.
PhylomeDBiQ9ZSA3.

Family and domain databases

InterProiView protein in InterPro
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom_sf.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ZSA3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNCCGSKPL TASDIVSDQK QETILGKPLE DIKKHYSFGD ELGKGNFGTT
60 70 80 90 100
YLCKENSTGK SYACKSIPKR TLSSEEEKEA VKTEIQIMDH VSGQPNIVQI
110 120 130 140 150
KGSYEDNNSI HIVMELCGGG ELFDKIDALV KSHSYYSEKD AAGIFRSIVN
160 170 180 190 200
AVKICHSLDV VHRDLKPENF LFSSKDENAM LKAIDFGCSV YIKEGKTFER
210 220 230 240 250
VVGSKYYIAP EVLEGSYGKE IDIWSAGVIL YILLSGVPPF QTGIESIIVS
260 270 280 290 300
TLCIVDAEIK ECRLDFESQP WPLISFKAKH LIGKMLTKKP KERISAADVL
310 320 330 340 350
EHPWMKSEAP DKPIDNVVLS RMKQFRAMNK LKKLALKVIA EGLSEEEIKG
360 370 380 390 400
LKTMFENMDM DKSGSITYEE LKMGLNRHGS KLSETEVKQL MEAADVDGNG
410 420 430 440 450
TIDYIEFISA TMHRHRLERD EHLYKAFQYF DKDGSGHITK EEVEIAMKEH
460 470 480 490
GMGDEANAKD LISEFDKNND GKIDYEEFCT MMRNGILQPQ GKLVGIHI
Length:498
Mass (Da):55,886
Last modified:February 10, 2009 - v2
Checksum:i101F3431C1FB2B5C
GO

Sequence cautioni

The sequence AAD03452 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB80836 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF118223 Genomic DNA. Translation: AAD03452.1. Sequence problems.
AL161501 Genomic DNA. Translation: CAB80836.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE82415.2.
PIRiC85059.
RefSeqiNP_001319866.1. NM_001340496.1.

Genome annotation databases

EnsemblPlantsiAT4G04710.1; AT4G04710.1; AT4G04710.
GeneIDi825806.
GrameneiAT4G04710.1; AT4G04710.1; AT4G04710.
KEGGiath:AT4G04710.

Similar proteinsi

Entry informationi

Entry nameiCDPKM_ARATH
AccessioniPrimary (citable) accession number: Q9ZSA3
Secondary accession number(s): F4JGW5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: February 10, 2009
Last modified: November 22, 2017
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families