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Protein

Calcium-dependent protein kinase 21

Gene

CPK21

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger. Mediates the phosphorylation and activation of the S-type anion efflux channel SLAC1.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei109ATPPROSITE-ProRule annotation1
Active sitei204Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi86 – 94ATPPROSITE-ProRule annotation9
Calcium bindingi393 – 4041PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi429 – 4402PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi465 – 4763PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi500 – 5114PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase 21 (EC:2.7.11.1)
Gene namesi
Name:CPK21
Ordered Locus Names:At4g04720
ORF Names:T4B21.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G04720.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • nucleus Source: GO_Central
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00003633432 – 531Calcium-dependent protein kinase 21Add BLAST530

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiQ9ZSA2.
PRIDEiQ9ZSA2.

PTM databases

iPTMnetiQ9ZSA2.
SwissPalmiQ9ZSA2.

Expressioni

Gene expression databases

GenevisibleiQ9ZSA2. AT.

Interactioni

Subunit structurei

Interacts with SLAC1 and ABI1.1 Publication

GO - Molecular functioni

  • calmodulin binding Source: GO_Central
  • protein phosphatase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi11118. 3 interactors.
DIPiDIP-59320N.
IntActiQ9ZSA2. 1 interactor.
STRINGi3702.AT4G04720.1.

Structurei

3D structure databases

ProteinModelPortaliQ9ZSA2.
SMRiQ9ZSA2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini80 – 338Protein kinasePROSITE-ProRule annotationAdd BLAST259
Domaini380 – 415EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini416 – 451EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini452 – 487EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini488 – 522EF-hand 4PROSITE-ProRule annotationAdd BLAST35

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni343 – 373Autoinhibitory domainBy similarityAdd BLAST31

Domaini

There is 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (343-373) inactivates kinase activity under calcium-free conditions (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation
Contains 4 EF-hand domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
InParanoidiQ9ZSA2.
KOiK13412.
OMAiTMFANID.
OrthoDBiEOG093609AJ.
PhylomeDBiQ9ZSA2.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ZSA2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCFSSKHRK TQNDGGEKSI PINPVQTHVV PEHRKPQTPT PKPMTQPIHQ
60 70 80 90 100
QISTPSSNPV SVRDPDTILG KPFEDIRKFY SLGKELGRGQ FGITYMCKEI
110 120 130 140 150
GTGNTYACKS ILKRKLISKQ DKEDVKREIQ IMQYLSGQPN IVEIKGAYED
160 170 180 190 200
RQSIHLVMEL CAGGELFDRI IAQGHYSERA AAGIIRSIVN VVQICHFMGV
210 220 230 240 250
VHRDLKPENF LLSSKEENAM LKATDFGLSV FIEEGKVYRD IVGSAYYVAP
260 270 280 290 300
EVLRRSYGKE IDIWSAGVIL YILLSGVPPF WAENEKGIFD EVIKGEIDFV
310 320 330 340 350
SEPWPSISES AKDLVRKMLT KDPKRRITAA QVLEHPWIKG GEAPDKPIDS
360 370 380 390 400
AVLSRMKQFR AMNKLKKLAL KVIAESLSEE EIKGLKTMFA NIDTDKSGTI
410 420 430 440 450
TYEELKTGLT RLGSRLSETE VKQLMEAADV DGNGTIDYYE FISATMHRYK
460 470 480 490 500
LDRDEHVYKA FQHFDKDNSG HITRDELESA MKEYGMGDEA SIKEVISEVD
510 520 530
TDNDGRINFE EFCAMMRSGS TQPQGKLLPF H
Length:531
Mass (Da):59,894
Last modified:May 1, 1999 - v1
Checksum:iD1DCCE1F3386F9D9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti468N → S in AAK92828 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF118223 Genomic DNA. Translation: AAD03453.1.
AL161501 Genomic DNA. Translation: CAB80837.1.
CP002687 Genomic DNA. Translation: AEE82416.1.
AY050891 mRNA. Translation: AAK92828.1.
AY150502 mRNA. Translation: AAN13018.1.
PIRiD85059.
RefSeqiNP_192381.1. NM_116710.3.
UniGeneiAt.24918.

Genome annotation databases

EnsemblPlantsiAT4G04720.1; AT4G04720.1; AT4G04720.
GeneIDi825807.
GrameneiAT4G04720.1; AT4G04720.1; AT4G04720.
KEGGiath:AT4G04720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF118223 Genomic DNA. Translation: AAD03453.1.
AL161501 Genomic DNA. Translation: CAB80837.1.
CP002687 Genomic DNA. Translation: AEE82416.1.
AY050891 mRNA. Translation: AAK92828.1.
AY150502 mRNA. Translation: AAN13018.1.
PIRiD85059.
RefSeqiNP_192381.1. NM_116710.3.
UniGeneiAt.24918.

3D structure databases

ProteinModelPortaliQ9ZSA2.
SMRiQ9ZSA2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi11118. 3 interactors.
DIPiDIP-59320N.
IntActiQ9ZSA2. 1 interactor.
STRINGi3702.AT4G04720.1.

PTM databases

iPTMnetiQ9ZSA2.
SwissPalmiQ9ZSA2.

Proteomic databases

PaxDbiQ9ZSA2.
PRIDEiQ9ZSA2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G04720.1; AT4G04720.1; AT4G04720.
GeneIDi825807.
GrameneiAT4G04720.1; AT4G04720.1; AT4G04720.
KEGGiath:AT4G04720.

Organism-specific databases

TAIRiAT4G04720.

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
InParanoidiQ9ZSA2.
KOiK13412.
OMAiTMFANID.
OrthoDBiEOG093609AJ.
PhylomeDBiQ9ZSA2.

Enzyme and pathway databases

BRENDAi2.7.11.1. 399.

Miscellaneous databases

PROiQ9ZSA2.

Gene expression databases

GenevisibleiQ9ZSA2. AT.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDPKL_ARATH
AccessioniPrimary (citable) accession number: Q9ZSA2
Secondary accession number(s): Q949U0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.