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Q9ZSA2

- CDPKL_ARATH

UniProt

Q9ZSA2 - CDPKL_ARATH

Protein

Calcium-dependent protein kinase 21

Gene

CPK21

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    May play a role in signal transduction pathways that involve calcium as a second messenger. Mediates the phosphorylation and activation of the S-type anion efflux channel SLAC1.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei109 – 1091ATPPROSITE-ProRule annotation
    Active sitei204 – 2041Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi86 – 949ATPPROSITE-ProRule annotation
    Calcium bindingi393 – 404121PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi429 – 440122PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi465 – 476123PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi500 – 511124PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calcium ion binding Source: InterPro
    3. protein binding Source: UniProtKB
    4. protein kinase activity Source: UniProtKB
    5. protein phosphatase binding Source: UniProtKB
    6. protein serine/threonine kinase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Calcium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT4G04720-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calcium-dependent protein kinase 21 (EC:2.7.11.1)
    Gene namesi
    Name:CPK21
    Ordered Locus Names:At4g04720
    ORF Names:T4B21.13
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G04720.

    Subcellular locationi

    Cell membrane 1 Publication; Lipid-anchor 1 Publication

    GO - Cellular componenti

    1. plasma membrane Source: TAIR
    2. plasmodesma Source: TAIR

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedSequence Analysis
    Chaini2 – 531530Calcium-dependent protein kinase 21PRO_0000363343Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycineSequence Analysis

    Keywords - PTMi

    Lipoprotein, Myristate

    Proteomic databases

    PaxDbiQ9ZSA2.
    PRIDEiQ9ZSA2.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9ZSA2.

    Interactioni

    Subunit structurei

    Interacts with SLAC1 and ABI1.1 Publication

    Protein-protein interaction databases

    BioGridi11118. 1 interaction.
    DIPiDIP-59320N.
    IntActiQ9ZSA2. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ZSA2.
    SMRiQ9ZSA2. Positions 42-517.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini80 – 338259Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini380 – 41536EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini416 – 45136EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini452 – 48736EF-hand 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini488 – 52235EF-hand 4PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni343 – 37331Autoinhibitory domainBy similarityAdd
    BLAST

    Domaini

    There is 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (343-373) inactivates kinase activity under calcium-free conditions By similarity.By similarity

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation
    Contains 4 EF-hand domains.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233030.
    InParanoidiQ9ZSA2.
    KOiK13412.
    OMAiNDGRINF.
    PhylomeDBiQ9ZSA2.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF13499. EF-hand_7. 2 hits.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00054. EFh. 4 hits.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00018. EF_HAND_1. 4 hits.
    PS50222. EF_HAND_2. 4 hits.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9ZSA2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGCFSSKHRK TQNDGGEKSI PINPVQTHVV PEHRKPQTPT PKPMTQPIHQ    50
    QISTPSSNPV SVRDPDTILG KPFEDIRKFY SLGKELGRGQ FGITYMCKEI 100
    GTGNTYACKS ILKRKLISKQ DKEDVKREIQ IMQYLSGQPN IVEIKGAYED 150
    RQSIHLVMEL CAGGELFDRI IAQGHYSERA AAGIIRSIVN VVQICHFMGV 200
    VHRDLKPENF LLSSKEENAM LKATDFGLSV FIEEGKVYRD IVGSAYYVAP 250
    EVLRRSYGKE IDIWSAGVIL YILLSGVPPF WAENEKGIFD EVIKGEIDFV 300
    SEPWPSISES AKDLVRKMLT KDPKRRITAA QVLEHPWIKG GEAPDKPIDS 350
    AVLSRMKQFR AMNKLKKLAL KVIAESLSEE EIKGLKTMFA NIDTDKSGTI 400
    TYEELKTGLT RLGSRLSETE VKQLMEAADV DGNGTIDYYE FISATMHRYK 450
    LDRDEHVYKA FQHFDKDNSG HITRDELESA MKEYGMGDEA SIKEVISEVD 500
    TDNDGRINFE EFCAMMRSGS TQPQGKLLPF H 531
    Length:531
    Mass (Da):59,894
    Last modified:May 1, 1999 - v1
    Checksum:iD1DCCE1F3386F9D9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti468 – 4681N → S in AAK92828. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF118223 Genomic DNA. Translation: AAD03453.1.
    AL161501 Genomic DNA. Translation: CAB80837.1.
    CP002687 Genomic DNA. Translation: AEE82416.1.
    AY050891 mRNA. Translation: AAK92828.1.
    AY150502 mRNA. Translation: AAN13018.1.
    PIRiD85059.
    RefSeqiNP_192381.1. NM_116710.2.
    UniGeneiAt.24918.

    Genome annotation databases

    EnsemblPlantsiAT4G04720.1; AT4G04720.1; AT4G04720.
    GeneIDi825807.
    KEGGiath:AT4G04720.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF118223 Genomic DNA. Translation: AAD03453.1 .
    AL161501 Genomic DNA. Translation: CAB80837.1 .
    CP002687 Genomic DNA. Translation: AEE82416.1 .
    AY050891 mRNA. Translation: AAK92828.1 .
    AY150502 mRNA. Translation: AAN13018.1 .
    PIRi D85059.
    RefSeqi NP_192381.1. NM_116710.2.
    UniGenei At.24918.

    3D structure databases

    ProteinModelPortali Q9ZSA2.
    SMRi Q9ZSA2. Positions 42-517.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 11118. 1 interaction.
    DIPi DIP-59320N.
    IntActi Q9ZSA2. 1 interaction.

    Proteomic databases

    PaxDbi Q9ZSA2.
    PRIDEi Q9ZSA2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G04720.1 ; AT4G04720.1 ; AT4G04720 .
    GeneIDi 825807.
    KEGGi ath:AT4G04720.

    Organism-specific databases

    TAIRi AT4G04720.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233030.
    InParanoidi Q9ZSA2.
    KOi K13412.
    OMAi NDGRINF.
    PhylomeDBi Q9ZSA2.

    Enzyme and pathway databases

    BioCyci ARA:AT4G04720-MONOMER.

    Gene expression databases

    Genevestigatori Q9ZSA2.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF13499. EF-hand_7. 2 hits.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00054. EFh. 4 hits.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00018. EF_HAND_1. 4 hits.
    PS50222. EF_HAND_2. 4 hits.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "The CDPK superfamily of protein kinases."
      Harmon A.C., Gribskov M., Gubrium E., Harper J.F.
      New Phytol. 151:175-183(2001)
      Cited for: GENE FAMILY, NOMENCLATURE.
    5. "Calcium signaling through protein kinases. The Arabidopsis calcium-dependent protein kinase gene family."
      Cheng S.-H., Willmann M.R., Chen H.-C., Sheen J.
      Plant Physiol. 129:469-485(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    6. Cited for: GENE FAMILY, NOMENCLATURE.
    7. "Subcellular targeting of nine calcium-dependent protein kinase isoforms from Arabidopsis."
      Dammann C., Ichida A., Hong B., Romanowsky S.M., Hrabak E.M., Harmon A.C., Pickard B.G., Harper J.F.
      Plant Physiol. 132:1840-1848(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    8. "Guard cell anion channel SLAC1 is regulated by CDPK protein kinases with distinct Ca2+ affinities."
      Geiger D., Scherzer S., Mumm P., Marten I., Ache P., Matschi S., Liese A., Wellmann C., Al-Rasheid K.A.S., Grill E., Romeis T., Hedrich R.
      Proc. Natl. Acad. Sci. U.S.A. 107:8023-8028(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SLAC1 AND ABI1, ENZYME REGULATION.

    Entry informationi

    Entry nameiCDPKL_ARATH
    AccessioniPrimary (citable) accession number: Q9ZSA2
    Secondary accession number(s): Q949U0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 10, 2009
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3