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Q9ZSA2

- CDPKL_ARATH

UniProt

Q9ZSA2 - CDPKL_ARATH

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Protein
Calcium-dependent protein kinase 21
Gene
CPK21, At4g04720, T4B21.13
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger. Mediates the phosphorylation and activation of the S-type anion efflux channel SLAC1.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity By similarity.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei109 – 1091ATP By similarity
Active sitei204 – 2041Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi86 – 949ATP By similarity
Calcium bindingi393 – 404121 By similarity
Add
BLAST
Calcium bindingi429 – 440122 By similarity
Add
BLAST
Calcium bindingi465 – 476123 By similarity
Add
BLAST
Calcium bindingi500 – 511124 By similarity
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calcium ion binding Source: InterPro
  3. protein binding Source: UniProtKB
  4. protein kinase activity Source: UniProtKB
  5. protein phosphatase binding Source: UniProtKB
  6. protein serine/threonine kinase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G04720-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase 21 (EC:2.7.11.1)
Gene namesi
Name:CPK21
Ordered Locus Names:At4g04720
ORF Names:T4B21.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G04720.

Subcellular locationi

Cell membrane; Lipid-anchor 1 Publication

GO - Cellular componenti

  1. plasma membrane Source: TAIR
  2. plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed Reviewed prediction
Chaini2 – 531530Calcium-dependent protein kinase 21
PRO_0000363343Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine Reviewed prediction

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiQ9ZSA2.
PRIDEiQ9ZSA2.

Expressioni

Gene expression databases

GenevestigatoriQ9ZSA2.

Interactioni

Subunit structurei

Interacts with SLAC1 and ABI1.1 Publication

Protein-protein interaction databases

BioGridi11118. 1 interaction.
DIPiDIP-59320N.
IntActiQ9ZSA2. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9ZSA2.
SMRiQ9ZSA2. Positions 42-517.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini80 – 338259Protein kinase
Add
BLAST
Domaini380 – 41536EF-hand 1
Add
BLAST
Domaini416 – 45136EF-hand 2
Add
BLAST
Domaini452 – 48736EF-hand 3
Add
BLAST
Domaini488 – 52235EF-hand 4
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni343 – 37331Autoinhibitory domain By similarity
Add
BLAST

Domaini

There is 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (343-373) inactivates kinase activity under calcium-free conditions By similarity.

Sequence similaritiesi

Contains 4 EF-hand domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233030.
InParanoidiQ9ZSA2.
KOiK13412.
OMAiNDGRINF.
PhylomeDBiQ9ZSA2.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ZSA2-1 [UniParc]FASTAAdd to Basket

« Hide

MGCFSSKHRK TQNDGGEKSI PINPVQTHVV PEHRKPQTPT PKPMTQPIHQ    50
QISTPSSNPV SVRDPDTILG KPFEDIRKFY SLGKELGRGQ FGITYMCKEI 100
GTGNTYACKS ILKRKLISKQ DKEDVKREIQ IMQYLSGQPN IVEIKGAYED 150
RQSIHLVMEL CAGGELFDRI IAQGHYSERA AAGIIRSIVN VVQICHFMGV 200
VHRDLKPENF LLSSKEENAM LKATDFGLSV FIEEGKVYRD IVGSAYYVAP 250
EVLRRSYGKE IDIWSAGVIL YILLSGVPPF WAENEKGIFD EVIKGEIDFV 300
SEPWPSISES AKDLVRKMLT KDPKRRITAA QVLEHPWIKG GEAPDKPIDS 350
AVLSRMKQFR AMNKLKKLAL KVIAESLSEE EIKGLKTMFA NIDTDKSGTI 400
TYEELKTGLT RLGSRLSETE VKQLMEAADV DGNGTIDYYE FISATMHRYK 450
LDRDEHVYKA FQHFDKDNSG HITRDELESA MKEYGMGDEA SIKEVISEVD 500
TDNDGRINFE EFCAMMRSGS TQPQGKLLPF H 531
Length:531
Mass (Da):59,894
Last modified:May 1, 1999 - v1
Checksum:iD1DCCE1F3386F9D9
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti468 – 4681N → S in AAK92828. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF118223 Genomic DNA. Translation: AAD03453.1.
AL161501 Genomic DNA. Translation: CAB80837.1.
CP002687 Genomic DNA. Translation: AEE82416.1.
AY050891 mRNA. Translation: AAK92828.1.
AY150502 mRNA. Translation: AAN13018.1.
PIRiD85059.
RefSeqiNP_192381.1. NM_116710.2.
UniGeneiAt.24918.

Genome annotation databases

EnsemblPlantsiAT4G04720.1; AT4G04720.1; AT4G04720.
GeneIDi825807.
KEGGiath:AT4G04720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF118223 Genomic DNA. Translation: AAD03453.1 .
AL161501 Genomic DNA. Translation: CAB80837.1 .
CP002687 Genomic DNA. Translation: AEE82416.1 .
AY050891 mRNA. Translation: AAK92828.1 .
AY150502 mRNA. Translation: AAN13018.1 .
PIRi D85059.
RefSeqi NP_192381.1. NM_116710.2.
UniGenei At.24918.

3D structure databases

ProteinModelPortali Q9ZSA2.
SMRi Q9ZSA2. Positions 42-517.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 11118. 1 interaction.
DIPi DIP-59320N.
IntActi Q9ZSA2. 1 interaction.

Proteomic databases

PaxDbi Q9ZSA2.
PRIDEi Q9ZSA2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G04720.1 ; AT4G04720.1 ; AT4G04720 .
GeneIDi 825807.
KEGGi ath:AT4G04720.

Organism-specific databases

TAIRi AT4G04720.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233030.
InParanoidi Q9ZSA2.
KOi K13412.
OMAi NDGRINF.
PhylomeDBi Q9ZSA2.

Enzyme and pathway databases

BioCyci ARA:AT4G04720-MONOMER.

Gene expression databases

Genevestigatori Q9ZSA2.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "The CDPK superfamily of protein kinases."
    Harmon A.C., Gribskov M., Gubrium E., Harper J.F.
    New Phytol. 151:175-183(2001)
    Cited for: GENE FAMILY, NOMENCLATURE.
  5. "Calcium signaling through protein kinases. The Arabidopsis calcium-dependent protein kinase gene family."
    Cheng S.-H., Willmann M.R., Chen H.-C., Sheen J.
    Plant Physiol. 129:469-485(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. Cited for: GENE FAMILY, NOMENCLATURE.
  7. "Subcellular targeting of nine calcium-dependent protein kinase isoforms from Arabidopsis."
    Dammann C., Ichida A., Hong B., Romanowsky S.M., Hrabak E.M., Harmon A.C., Pickard B.G., Harper J.F.
    Plant Physiol. 132:1840-1848(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. "Guard cell anion channel SLAC1 is regulated by CDPK protein kinases with distinct Ca2+ affinities."
    Geiger D., Scherzer S., Mumm P., Marten I., Ache P., Matschi S., Liese A., Wellmann C., Al-Rasheid K.A.S., Grill E., Romeis T., Hedrich R.
    Proc. Natl. Acad. Sci. U.S.A. 107:8023-8028(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SLAC1 AND ABI1, ENZYME REGULATION.

Entry informationi

Entry nameiCDPKL_ARATH
AccessioniPrimary (citable) accession number: Q9ZSA2
Secondary accession number(s): Q949U0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: May 1, 1999
Last modified: June 11, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi