Reviewed, UniProtKB/Swiss-Prot Q9ZS40 (CHS2_DAUCA)

Names and origin

Protein names

Recommended name:
    Chalcone synthase 2
    EC=2.3.1.74
Alternative name(s):
    Naringenin-chalcone synthase 2
    DcCHS2

Gene names

Name:

CHS2

Organism

Daucus carota (Carrot)

Taxonomic identifier

4039 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Apiales › Apiaceae › Apioideae › Scandiceae › Daucinae › Daucus

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.
Catalytic activity	3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Sequence similarities	Belongs to the chalcone/stilbene synthases family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	acyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW naringenin-chalcone synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 397	397	Chalcone synthase 2		PRO_0000215971
Sites
	Active site	168	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZS40-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 9386F44B9132EDEE	FASTA	397	43,559
	10 20 30 40 50 60 MANHNAEIEE IRKRQRAQGP ANILAIGTAT PSNCVYQADY PDYYFRITNS EHMSDLKLKF 70 80 90 100 110 120 KRMCEKSMIR KRYMHITEEY LKENPNVCAY EAPSLDARQD LVVVEVPRLG KEAAAKAIKE 130 140 150 160 170 180 WGHPKSKITH LIFCTTSGVD MPGADYQLTK LLGLRPSVKR FMMYQQGCFA GGTVLRLAKD 190 200 210 220 230 240 LAENNTGARV LVVCSEITAV TFRGPSDSHL DSLVGQALFG DGAAAVIVGS DPDLSVERPL 250 260 270 280 290 300 FQLISAAQTI LPDSDGAIDG HLREVGLTFH LLKDVPGLIS KNIEKSLKEA FGPIGISDWN 310 320 330 340 350 360 SLFWIAHPGG PAILDQVELK LGLKEEKMRA TRQVLSDYGN MSSACVLFIL DEMRKKSIEE 370 380 390 GKATTGDGLD WGVLFGFGPG LTVETVVLHS VPATITH

References

[1]	"Isoforms of chalcone synthase in Daucus carota L. and their differential expression in organs from the European wild carrot and in ultraviolet-A-irradiated cell cultures." Hirner A.A., Seitz H.U. Planta 210:993-998(2000) [PubMed: 10872233] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ006780 mRNA. Translation: CAA07245.1.
3D structure databases
SMR	Q9ZS40. Positions 8-391.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.3.1.74. 1081.
Family and domain databases
InterPro	IPR012328. Chalcone/stilbene_synth_C. IPR018088. Chalcone/stilbene_synthase_AS. IPR001099. Chalcone/stilbene_synthase_N. IPR011141. Polyketide_synthase_type-III. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 2 hits.
Pfam	PF02797. Chal_sti_synt_C. 1 hit. PF00195. Chal_sti_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000451. PKS_III. 1 hit.
PROSITE	PS00441. CHALCONE_SYNTH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHS2_DAUCA

Accession

Primary (citable) accession number: Q9ZS40

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	May 1, 1999
Last modified:	November 24, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)