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Protein

DEAD-box ATP-dependent RNA helicase 28

Gene

RH28

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi211 – 218ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DEAD-box ATP-dependent RNA helicase 28 (EC:3.6.4.13)
Gene namesi
Name:RH28
Ordered Locus Names:At4g16630
ORF Names:dl4340c, FCAALL.424
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G16630
TAIRilocus:2130918 AT4G16630

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002391681 – 789DEAD-box ATP-dependent RNA helicase 28Add BLAST789

Proteomic databases

PaxDbiQ9ZRZ8

PTM databases

iPTMnetiQ9ZRZ8

Expressioni

Gene expression databases

ExpressionAtlasiQ9ZRZ8 baseline and differential
GenevisibleiQ9ZRZ8 AT

Interactioni

Protein-protein interaction databases

BioGridi12657, 4 interactors
IntActiQ9ZRZ8, 4 interactors
STRINGi3702.AT4G16630.1

Structurei

3D structure databases

ProteinModelPortaliQ9ZRZ8
SMRiQ9ZRZ8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini198 – 372Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST175
Domaini402 – 546Helicase C-terminalPROSITE-ProRule annotationAdd BLAST145

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili13 – 66Sequence analysisAdd BLAST54
Coiled coili110 – 135Sequence analysisAdd BLAST26
Coiled coili563 – 591Sequence analysisAdd BLAST29
Coiled coili628 – 677Sequence analysisAdd BLAST50

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi167 – 195Q motifAdd BLAST29
Motifi320 – 323DEAD box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi14 – 148Glu-richAdd BLAST135

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0338 Eukaryota
COG0513 LUCA
HOGENOMiHOG000265456
InParanoidiQ9ZRZ8
KOiK13181
OMAiSKPRNQP
OrthoDBiEOG09360564
PhylomeDBiQ9ZRZ8

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequencei

Sequence statusi: Complete.

Q9ZRZ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSSFFFEDA SDDELELIRN QEDSSEEDVK EGEAEEHEAG EDEDGEEEYE
60 70 80 90 100
EEDDDEEEED EKRKRDADAQ SPWDFASYSS SVGEEHARRH TTSIDEKISK
110 120 130 140 150
AIQHRPVPIS INEEEEEEEE EEDASDAETD KQEEYLSEDD EAAEYKPEDA
160 170 180 190 200
TPKPFFSTVD GVSFHADTFM ELNLSRPLLR ACETLGYKKP TPIQAACIPL
210 220 230 240 250
ALTGRDLCAS AITGSGKTAA FALPTLERLL FRPKRVFATR VLILTPTREL
260 270 280 290 300
AVQIHSMIQN LAQFTDIKCG LIVGGLSVRE QEVVLRSMPD IVVATPGRMI
310 320 330 340 350
DHLRNSMSVD LDDLAVLILD EADRLLQTGF ATEITELVRL CPKRRQTMLF
360 370 380 390 400
SATMTEEVKE LVKLSLNKPL RLSADPSARR PPGLTEEVVR IRRTREANQE
410 420 430 440 450
AVLLSLCTRT FKSKVIIFSG TKQAAHRLKI LFGLAGLKAA ELHGNLTQAQ
460 470 480 490 500
RLDSLELFRK QEVDFLIATD VAARGLDIIG VQTVINYACP REIDSYVHRV
510 520 530 540 550
GRTARAGREG YAVTFVTDSD RSLLKVIAKK VGSKLKSRVI PEQSIVKWSQ
560 570 580 590 600
IIDEMEDQYS AVISAERDER ALRKAEMEFA KAENMLEHRD EIYARPKRTW
610 620 630 640 650
FMTEKEKKLV AQAEKDSAGN PAGGELVSAD RAEDLKMKEK RKREREKNLP
660 670 680 690 700
RKKRRKLEAA REMLEDNEGE EEEEDEEGDE KRGRSRGKDK KKQETDKKGL
710 720 730 740 750
TLKDLGYMRA KAVKAKQRAI DSGKMERPKP DKKQSRSKPR NQPRGEEMKD
760 770 780
LFKSDMGEKK QGRGGAAAAA KPRTKSKNSF KSKARYKRR
Length:789
Mass (Da):89,355
Last modified:May 1, 1999 - v1
Checksum:iDF1CCAB61A4DD66D
GO

Sequence cautioni

The sequence CAB10438 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB78705 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010475 mRNA Translation: CAA09214.1
Z97341 Genomic DNA Translation: CAB10438.1 Sequence problems.
AL161544 Genomic DNA Translation: CAB78705.1 Sequence problems.
CP002687 Genomic DNA Translation: AEE83778.1
AK228353 mRNA Translation: BAF00292.1
PIRiD71433
T51310
RefSeqiNP_193396.3, NM_117764.5
UniGeneiAt.49

Genome annotation databases

EnsemblPlantsiAT4G16630.1; AT4G16630.1; AT4G16630
GeneIDi827364
GrameneiAT4G16630.1; AT4G16630.1; AT4G16630
KEGGiath:AT4G16630

Similar proteinsi

Entry informationi

Entry nameiRH28_ARATH
AccessioniPrimary (citable) accession number: Q9ZRZ8
Secondary accession number(s): O23506, Q0WRF7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: May 1, 1999
Last modified: April 25, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health