Reviewed, UniProtKB/Swiss-Prot Q9ZRI8 (FDH_HORVU)

Names and origin

Protein names	Recommended name:     Formate dehydrogenase, mitochondrial     EC=1.2.1.2 Alternative name(s):     NAD-dependent formate dehydrogenase       Short name=FDH
Organism	Hordeum vulgare (Barley)
Taxonomic identifier	4513 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Formate + NAD+ = CO2 + NADH.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. FDH subfamily.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro formate dehydrogenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 21	21	Mitochondrion By similarity
	Chain	22 – 377	356	Formate dehydrogenase, mitochondrial		PRO_0000007194
Sites
	Active site	283	1	By similarity
	Active site	331	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZRI8-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: E28C1FE24E9225C7	FASTA	377	41,546
	10 20 30 40 50 60 MAAMWRAAAR QLVDRAVGSR AAHTSAGSKK IVGVFYQAGE YADKNPNFVG CVEGALGIRD 70 80 90 100 110 120 WLESKGHHYI VTDDKEGFNS ELEKHIEDMH VLITTPFHPA YVTAEKIKKA KTPELLLTAG 130 140 150 160 170 180 IGSDHIDLPA AAAAGLTVAR VTGSNTVSVA EDELMRILIL LRNFLPGYQQ VVKGEWNVAG 190 200 210 220 230 240 IAHRAYDLEG KTVGTVGAGR YGRLLLQRLK PFNCNLLYHD RLQINPELEK EIGAKFEEDL 250 260 270 280 290 300 DAMLPKCDVV VINTPLTEKT RGMFNKEKIA KMKKGVIIVN NARGAIMDTQ AVADACSSGH 310 320 330 340 350 360 IAGYGGDVWF PQPAPKDHPW RYMPNHAMTP HISGTTIDAQ LRYAAGVKDM LDRYFKGEEF 370 PVENYIVKEG ELASQYK

References

[1]

"Formate dehydrogenase, an enzyme of anaerobic metabolism, is induced by iron deficiency in barley roots." Suzuki K., Itai R., Suzuki K., Nakanishi H., Nishizawa N.K., Yoshimura E., Mori S. Plant Physiol. 116:725-732(1998) [PubMed: 9489019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Ehimehadaka No.1. Tissue: Root.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D88272 mRNA. Translation: BAA36181.1.
3D structure databases
HSSP	HSSP built from PDB template 2NAD based on UniProtKB P33160.
ModBase	Search...
Organism-specific databases
Gramene	Q9ZRI8.
Enzyme and pathway databases
BRENDA	1.2.1.2. 283.
Family and domain databases
InterPro	IPR006139. D-isomer_2_OHA_DH. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FDH_HORVU

Accession

Primary (citable) accession number: Q9ZRI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)