Q9ZRE2 (RABD1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein RABD1
Short name=AtRABD1

Alternative name(s):
Ras-related protein ATFP8

Gene names

Name:	RABD1
Synonyms:	ATFP8
Ordered Locus Names:	At3g11730
ORF Names:	F26K24.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	205 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Protein transport. Regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER).
Subunit structure	Does not interact with GC5.
Subcellular location	Golgi apparatus › trans-Golgi network membrane. Golgi apparatus membrane; Lipid-anchor Probable Ref.8.
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Golgi apparatus Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER to Golgi vesicle-mediated transport Inferred from mutant phenotype Ref.8. Source: UniProtKB protein transport Inferred from electronic annotation. Source: UniProtKB-KW small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi membrane Inferred from direct assay Ref.8. Source: UniProtKB plasma membrane Inferred from direct assay PubMed 17644812. Source: TAIR trans-Golgi network membrane Inferred from direct assay Ref.8. Source: UniProtKB
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MYA2	Q9LKB9	3	EBI-2009542,EBI-2009528

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 205	205	Ras-related protein RABD1		PRO_0000348544
Regions
	Nucleotide binding	15 – 22	8	GTP By similarity
	Nucleotide binding	63 – 67	5	GTP By similarity
	Nucleotide binding	121 – 124	4	GTP By similarity
	Nucleotide binding	151 – 152	2	GTP By similarity
	Motif	37 – 45	9	Effector region By similarity
Amino acid modifications
	Lipidation	202	1	S-geranylgeranyl cysteine By similarity
	Lipidation	203	1	S-geranylgeranyl cysteine By similarity
Experimental info
	Mutagenesis	22	1	S → N: Dominant negative (GDP-bound form); no effect on trafficking between the ER and Golgi. Ref.8
	Mutagenesis	67	1	Q → L: No effect on trafficking between the ER and Golgi. Ref.8
	Mutagenesis	121	1	N → I: Inhibits trafficking between the ER and Golgi. Causes severe dwarfism and seedlings death. Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q9ZRE2 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 8842C2B074B71AAD	FASTA	205	22,725
	10 20 30 40 50 60 MSNEYDYLFK LLLIGDSSVG KSCLLLRFAD DAYIDSYIST IGVDFKIRTI EQDGKTIKLQ 70 80 90 100 110 120 IWDTAGQERF RTITSSYYRG AHGIIIVYDC TEMESFNNVK QWLSEIDRYA NESVCKLLIG 130 140 150 160 170 180 NKNDMVESKV VSTETGRALA DELGIPFLET SAKDSINVEQ AFLTIAGEIK KKMGSQTNAN 190 200 KTSGPGTVQM KGQPIQQNNG GCCGQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and isoprenylation of plant GTP-binding proteins." Biermann B.J., Randall S.K., Crowell D.N. Plant Mol. Biol. 31:1021-1028(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], IN VITRO ISOPRENYLATION.
[2]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Analysis of the small GTPase gene superfamily of Arabidopsis." Vernoud V., Horton A.C., Yang Z., Nielsen E. Plant Physiol. 131:1191-1208(2003) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[7]	"Localization and domain characterization of Arabidopsis golgin candidates." Latijnhouwers M., Gillespie T., Boevink P., Kriechbaumer V., Hawes C., Carvalho C.M. J. Exp. Bot. 58:4373-4386(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GC5.
[8]	"Genetic evidence that the higher plant Rab-D1 and Rab-D2 GTPases exhibit distinct but overlapping interactions in the early secretory pathway." Pinheiro H., Samalova M., Geldner N., Chory J., Martinez A., Moore I. J. Cell Sci. 122:3749-3758(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF SER-22; GLN-67 AND ASN-121.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U64911 mRNA. Translation: AAD00111.1. AC016795 Genomic DNA. Translation: AAF23189.1. CP002686 Genomic DNA. Translation: AEE75090.1. AK118150 mRNA. Translation: BAC42775.1. BT005576 mRNA. Translation: AAO63996.1.
IPI	IPI00547722.
RefSeq	NP_187779.1. NM_112005.3.
UniGene	At.18731.
3D structure databases
ProteinModelPortal	Q9ZRE2.
SMR	Q9ZRE2. Positions 3-193.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9ZRE2. 9 interactions.
STRING	3702.AT3G11730.1-P.
Proteomic databases
PaxDb	Q9ZRE2.
PRIDE	Q9ZRE2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G11730.1; AT3G11730.1; AT3G11730.
GeneID	820345.
KEGG	ath:AT3G11730.
Organism-specific databases
TAIR	At3g11730.
Phylogenomic databases
eggNOG	COG1100.
HOGENOM	HOG000233968.
InParanoid	Q9ZRE2.
KO	K07976.
OMA	AKDFADS.
PhylomeDB	Q9ZRE2.
ProtClustDB	CLSN2684436.
Gene expression databases
Genevestigator	Q9ZRE2.
Family and domain databases
InterPro	IPR027417. P-loop_NTPase. IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR003579. Small_GTPase_Rab_type. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00175. RAB. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51419. RAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RABD1_ARATH

Accession

Primary (citable) accession number: Q9ZRE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	May 1, 1999
Last modified:	May 29, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program