Q9ZRA2 (HGD_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homogentisate 1,2-dioxygenase
EC=1.13.11.5

Alternative name(s):
Homogentisate oxygenase
Homogentisic acid oxidase
Homogentisicase

Gene names

Name:	HGO
Ordered Locus Names:	At5g54080
ORF Names:	MJP23.6

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Homogentisate + O2 = 4-maleylacetoacetate.
Cofactor	Iron.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6.
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway tyrosine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytosol Inferred from direct assay PubMed 21166475. Source: TAIR
Molecular_function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	Homogentisate 1,2-dioxygenase		PRO_0000220244
Sites
	Metal binding	341	1	Iron By similarity
	Metal binding	347	1	Iron By similarity
	Metal binding	377	1	Iron By similarity
Experimental info
	Sequence conflict	7	1	E → K in AAM65958. Ref.6
	Sequence conflict	69	1	V → I in AAM65958. Ref.6
	Sequence conflict	116	1	I → T in AAM65958. Ref.6
	Sequence conflict	146	1	T → K in AAM65958. Ref.6
	Sequence conflict	181	1	T → S in AAM65958. Ref.6
	Sequence conflict	231	1	S → P in AAM65958. Ref.6
	Sequence conflict	428	1	D → E in AAD00360. Ref.1
	Sequence conflict	452	1	S → P in AAM65958. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q9ZRA2 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: CD90DD9638014D31	FASTA	461	51,455
	10 20 30 40 50 60 MEEKKKELEE LKYQSGFGNH FSSEAIAGAL PLDQNSPLLC PYGLYAEQIS GTSFTSPRKL 70 80 90 100 110 120 NQRSWLYRVK PSVTHEPFKP RVPAHKKLVS EFDASNSRTN PTQLRWRPED IPDSEIDFVD 130 140 150 160 170 180 GLFTICGAGS SFLRHGFAIH MYVANTGMKD SAFCNADGDF LLVPQTGRLW IETECGRLLV 190 200 210 220 230 240 TPGEIAVIPQ GFRFSIDLPD GKSRGYVAEI YGAHFQLPDL GPIGANGLAA SRDFLAPTAW 250 260 270 280 290 300 FEDGLRPEYT IVQKFGGELF TAKQDFSPFN VVAWHGNYVP YKYDLKKFCP YNTVLLDHGD 310 320 330 340 350 360 PSINTVLTAP TDKPGVALLD FVIFPPRWLV AEHTFRPPYY HRNCMSEFMG LIYGAYEAKA 370 380 390 400 410 420 DGFLPGGASL HSCMTPHGPD TTTYEATIAR VNAMAPSKLT GTMAFMFESA LIPRVCHWAL 430 440 450 460 ESPFLDHDYY QCWIGLKSHF SRISLDKTNV ESTEKEPGAS E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the homogentisate 1,2-dioxygenase gene in A. thaliana and C. elegans." Schmidt S.R., Werner E., Mueller C.R., Kress W. Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	Schmidt S.R. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U80668 mRNA. Translation: AAD00360.1. AF130845 Genomic DNA. Translation: AAF36499.1. AB018115 Genomic DNA. Translation: BAA97130.1. CP002688 Genomic DNA. Translation: AED96447.1. CP002688 Genomic DNA. Translation: AED96448.1. AY140075 mRNA. Translation: AAM98216.1. BT010329 mRNA. Translation: AAQ55280.1. AY088421 mRNA. Translation: AAM65958.1.
IPI	IPI00527594.
RefSeq	NP_200219.1. NM_124787.3. NP_851187.1. NM_180856.3.
UniGene	At.23160. At.72265.
3D structure databases
ProteinModelPortal	Q9ZRA2.
SMR	Q9ZRA2. Positions 10-441.
ModBase	Search...
Proteomic databases
PaxDb	Q9ZRA2.
PRIDE	Q9ZRA2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G54080.1; AT5G54080.1; AT5G54080. AT5G54080.2; AT5G54080.2; AT5G54080.
GeneID	835494.
KEGG	ath:AT5G54080.
Organism-specific databases
TAIR	At5g54080.
Phylogenomic databases
eggNOG	COG3508.
HOGENOM	HOG000139824.
InParanoid	Q9ZRA2.
KO	K00451.
OMA	AVTKWGL.
PhylomeDB	Q9ZRA2.
ProtClustDB	PLN02658.
Enzyme and pathway databases
BRENDA	1.13.11.5. 399.
UniPathway	UPA00139; UER00339.
Gene expression databases
Genevestigator	Q9ZRA2.
GermOnline	AT5G54080. Arabidopsis thaliana.
Family and domain databases
Gene3D	2.60.120.10. 2 hits.
InterPro	IPR005708. Homogentis_dOase. IPR014710. RmlC-like_jellyroll. IPR011051. RmlC_Cupin. [Graphical view]
PANTHER	PTHR11056. PTHR11056. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_ARATH

Accession

Primary (citable) accession number: Q9ZRA2
Secondary accession number(s): Q8L9I0, Q9LDB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	April 27, 2001
Last modified:	May 1, 2013
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program