Reviewed, UniProtKB/Swiss-Prot Q9ZRA2 (HGD_ARATH)

Names and origin

Protein names

Recommended name:
    Homogentisate 1,2-dioxygenase
    EC=1.13.11.5
Alternative name(s):
    Homogentisic acid oxidase
    Homogentisate oxygenase
    Homogentisicase

Gene names

Name:	HGO
Ordered Locus Names:	At5g54080
ORF Names:	MJP23.6

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Homogentisate + O(2) = 4-maleylacetoacetate.
Cofactor	Iron.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetic acid and fumarate from L-phenylalanine: step 4/6.
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	Homogentisate 1,2-dioxygenase		PRO_0000220244
Sites
	Metal binding	341	1	Iron By similarity
	Metal binding	347	1	Iron By similarity
	Metal binding	377	1	Iron By similarity
Experimental info
	Sequence conflict	7	1	E → K in AAM65958. Ref.5
	Sequence conflict	69	1	V → I in AAM65958. Ref.5
	Sequence conflict	116	1	I → T in AAM65958. Ref.5
	Sequence conflict	146	1	T → K in AAM65958. Ref.5
	Sequence conflict	181	1	T → S in AAM65958. Ref.5
	Sequence conflict	231	1	S → P in AAM65958. Ref.5
	Sequence conflict	428	1	D → E in AAD00360. Ref.1
	Sequence conflict	452	1	S → P in AAM65958. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q9ZRA2-1 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: CD90DD9638014D31	FASTA	461	51,455
	10 20 30 40 50 60 MEEKKKELEE LKYQSGFGNH FSSEAIAGAL PLDQNSPLLC PYGLYAEQIS GTSFTSPRKL 70 80 90 100 110 120 NQRSWLYRVK PSVTHEPFKP RVPAHKKLVS EFDASNSRTN PTQLRWRPED IPDSEIDFVD 130 140 150 160 170 180 GLFTICGAGS SFLRHGFAIH MYVANTGMKD SAFCNADGDF LLVPQTGRLW IETECGRLLV 190 200 210 220 230 240 TPGEIAVIPQ GFRFSIDLPD GKSRGYVAEI YGAHFQLPDL GPIGANGLAA SRDFLAPTAW 250 260 270 280 290 300 FEDGLRPEYT IVQKFGGELF TAKQDFSPFN VVAWHGNYVP YKYDLKKFCP YNTVLLDHGD 310 320 330 340 350 360 PSINTVLTAP TDKPGVALLD FVIFPPRWLV AEHTFRPPYY HRNCMSEFMG LIYGAYEAKA 370 380 390 400 410 420 DGFLPGGASL HSCMTPHGPD TTTYEATIAR VNAMAPSKLT GTMAFMFESA LIPRVCHWAL 430 440 450 460 ESPFLDHDYY QCWIGLKSHF SRISLDKTNV ESTEKEPGAS E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the homogentisate 1,2-dioxygenase gene in A. thaliana and C. elegans." Schmidt S.R., Werner E., Mueller C.R., Kress W. Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	Schmidt S.R. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed: 10718197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U80668 mRNA. Translation: AAD00360.1. AF130845 Genomic DNA. Translation: AAF36499.1. AB018115 Genomic DNA. Translation: BAA97130.1. AY140075 mRNA. Translation: AAM98216.1. BT010329 mRNA. Translation: AAQ55280.1. AY088421 mRNA. Translation: AAM65958.1.
RefSeq	NP_200219.1. NP_851187.1.
UniGene	At.23160
3D structure databases
HSSP	HSSP built from PDB template 1EYB based on UniProtKB Q93099.
ModBase	Search...
Genome annotation databases
GeneID	835494.
GenomeReviews	Gene locus AT5G54080 in contig BA000015_GR.
KEGG	ath:AT5G54080.
NMPDR	fig|3702.1.peg.27312.
Organism-specific databases
TAIR	At5g54080.
Gene expression databases
ArrayExpress	Q9ZRA2.
GermOnline	AT5G54080. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR005708. Homogentis_dOase. [Graphical view]
PANTHER	PTHR11056. Homogentis_dOase. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01015. hmgA. 1 hit.
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

HGD_ARATH

Accession

Primary (citable) accession number: Q9ZRA2
Secondary accession number(s): Q8L9I0, Q9LDB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	April 27, 2001
Last modified:	November 4, 2008
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)