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Protein

ABC transporter B family member 1

Gene

ABCB1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Auxin efflux transporter that acts as a negative regulator of light signaling to promote hypocotyl elongation. Mediates the accumulation of chlorophyll and anthocyanin, as well as the expression of genes in response to light. Participates directly in auxin efflux and thus regulates the polar (presumably basipetal) auxin transport (from root tips to root elongating zone). Transports also some auxin metabolites such as oxindoleacetic acid and indoleacetaldehyde. Involved in divers auxin-mediated responses including gravitropism, phototropism and lateral root formation. Confers resistance to herbicides such as dicamba, pendimethalin, oryzalin, and monosodium acid methanearsonate (MSMA), but not to herbicides such as glyphosate, atrazine, bentazon and fluazifop-p-butyl. Mediates also resistance to xenobiotics such as cycloheximide and the cytokinin N6-(2-isopentenyl)adenine (2IP).7 Publications

Enzyme regulationi

Transport activity inhibited by 1-N-naphthylphthalamic acid (NPA), cyclopropyl propane dione (CPD), cyclosporin A, verapamil and quercetin.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi403 – 4108ATP 1PROSITE-ProRule annotation
Nucleotide bindingi1059 – 10668ATP 2PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • anthocyanin accumulation in tissues in response to UV light Source: TAIR
  • auxin-activated signaling pathway Source: UniProtKB-KW
  • auxin efflux Source: TAIR
  • auxin polar transport Source: TAIR
  • photomorphogenesis Source: TAIR
  • positive gravitropism Source: TAIR
  • regulation of cell size Source: TAIR
  • response to auxin Source: TAIR
  • response to blue light Source: TAIR
  • response to nematode Source: TAIR
  • response to red or far red light Source: TAIR
  • stamen development Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Auxin signaling pathway, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G36910-MONOMER.

Protein family/group databases

TCDBi3.A.1.201.5. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
ABC transporter B family member 1
Short name:
ABC transporter ABCB.1
Short name:
AtABCB1
Alternative name(s):
Multidrug resistance protein 1
P-glycoprotein 1
Short name:
AtPgp1
Gene namesi
Name:ABCB1
Synonyms:MDR1, PGP1
Ordered Locus Names:At2g36910
ORF Names:T1J8.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G36910.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei42 – 6221HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei93 – 11321HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei166 – 18621HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei187 – 20721HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei277 – 29721HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei301 – 32121HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei705 – 72521HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei745 – 76521HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei821 – 84323HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei845 – 86723HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei932 – 95221HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei967 – 98721HelicalPROSITE-ProRule annotationAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: GO_Central
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12861286ABC transporter B family member 1PRO_0000227912Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi217 – 2171N-linked (GlcNAc...)Sequence analysis
Glycosylationi640 – 6401N-linked (GlcNAc...)Sequence analysis
Glycosylationi771 – 7711N-linked (GlcNAc...)Sequence analysis
Glycosylationi797 – 7971N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9ZR72.
PRIDEiQ9ZR72.

PTM databases

iPTMnetiQ9ZR72.

Expressioni

Tissue specificityi

Ubiquitous, with high levels in peduncles. Mostly localized in young developing tissues, including meristems, as well as root and shoot apices.3 Publications

Inductioni

By auxin.1 Publication

Gene expression databases

GenevisibleiQ9ZR72. AT.

Interactioni

Subunit structurei

Interacts with 1-naphthylphthalamic acid (NPA) and FKBP42/TWD1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
FKBP42Q9LDC02EBI-366396,EBI-360006

Protein-protein interaction databases

BioGridi3609. 10 interactions.
IntActiQ9ZR72. 3 interactions.
MINTiMINT-8064482.
STRINGi3702.AT2G36910.1.

Structurei

3D structure databases

ProteinModelPortaliQ9ZR72.
SMRiQ9ZR72. Positions 26-644, 704-1264.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini44 – 333290ABC transmembrane type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini368 – 604237ABC transporter 1PROSITE-ProRule annotationAdd
BLAST
Domaini700 – 988289ABC transmembrane type-1 2PROSITE-ProRule annotationAdd
BLAST
Domaini1024 – 1260237ABC transporter 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1049 – 1286238Interaction with FKBP42/TWD1Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi8 – 125Poly-Pro
Compositional biasi621 – 66848Ser-richAdd
BLAST

Sequence similaritiesi

Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0055. Eukaryota.
COG1132. LUCA.
InParanoidiQ9ZR72.
KOiK05658.
OMAiRISVIMQ.
PhylomeDBiQ9ZR72.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZR72-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNDGGAPPP PPTLVVEEPK KAEIRGVAFK ELFRFADGLD YVLMGIGSVG
60 70 80 90 100
AFVHGCSLPL FLRFFADLVN SFGSNSNNVE KMMEEVLKYA LYFLVVGAAI
110 120 130 140 150
WASSWAEISC WMWSGERQTT KMRIKYLEAA LNQDIQFFDT EVRTSDVVFA
160 170 180 190 200
INTDAVMVQD AISEKLGNFI HYMATFVSGF IVGFTAVWQL ALVTLAVVPL
210 220 230 240 250
IAVIGGIHTT TLSKLSNKSQ ESLSQAGNIV EQTVVQIRVV MAFVGESRAS
260 270 280 290 300
QAYSSALKIA QKLGYKTGLA KGMGLGATYF VVFCCYALLL WYGGYLVRHH
310 320 330 340 350
LTNGGLAIAT MFAVMIGGLA LGQSAPSMAA FAKAKVAAAK IFRIIDHKPT
360 370 380 390 400
IERNSESGVE LDSVTGLVEL KNVDFSYPSR PDVKILNNFC LSVPAGKTIA
410 420 430 440 450
LVGSSGSGKS TVVSLIERFY DPNSGQVLLD GQDLKTLKLR WLRQQIGLVS
460 470 480 490 500
QEPALFATSI KENILLGRPD ADQVEIEEAA RVANAHSFII KLPDGFDTQV
510 520 530 540 550
GERGLQLSGG QKQRIAIARA MLKNPAILLL DEATSALDSE SEKLVQEALD
560 570 580 590 600
RFMIGRTTLI IAHRLSTIRK ADLVAVLQQG SVSEIGTHDE LFSKGENGVY
610 620 630 640 650
AKLIKMQEAA HETAMSNARK SSARPSSARN SVSSPIMTRN SSYGRSPYSR
660 670 680 690 700
RLSDFSTSDF SLSIDASSYP NYRNEKLAFK DQANSFWRLA KMNSPEWKYA
710 720 730 740 750
LLGSVGSVIC GSLSAFFAYV LSAVLSVYYN PDHEYMIKQI DKYCYLLIGL
760 770 780 790 800
SSAALVFNTL QHSFWDIVGE NLTKRVREKM LSAVLKNEMA WFDQEENESA
810 820 830 840 850
RIAARLALDA NNVRSAIGDR ISVIVQNTAL MLVACTAGFV LQWRLALVLV
860 870 880 890 900
AVFPVVVAAT VLQKMFMTGF SGDLEAAHAK GTQLAGEAIA NVRTVAAFNS
910 920 930 940 950
EAKIVRLYTA NLEPPLKRCF WKGQIAGSGY GVAQFCLYAS YALGLWYASW
960 970 980 990 1000
LVKHGISDFS KTIRVFMVLM VSANGAAETL TLAPDFIKGG QAMRSVFELL
1010 1020 1030 1040 1050
DRKTEIEPDD PDTTPVPDRL RGEVELKHID FSYPSRPDIQ IFRDLSLRAR
1060 1070 1080 1090 1100
AGKTLALVGP SGCGKSSVIS LIQRFYEPSS GRVMIDGKDI RKYNLKAIRK
1110 1120 1130 1140 1150
HIAIVPQEPC LFGTTIYENI AYGHECATEA EIIQAATLAS AHKFISALPE
1160 1170 1180 1190 1200
GYKTYVGERG VQLSGGQKQR IAIARALVRK AEIMLLDEAT SALDAESERS
1210 1220 1230 1240 1250
VQEALDQACS GRTSIVVAHR LSTIRNAHVI AVIDDGKVAE QGSHSHLLKN
1260 1270 1280
HPDGIYARMI QLQRFTHTQV IGMTSGSSSR VKEDDA
Length:1,286
Mass (Da):140,573
Last modified:May 1, 1999 - v1
Checksum:iA6C90AF42CC1FF01
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti293 – 2931G → D in AAM98246 (PubMed:14593172).Curated
Sequence conflicti1188 – 11881E → G in AAM98246 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61370 Genomic DNA. Translation: CAA43646.1.
AC006922 Genomic DNA. Translation: AAD31576.1.
CP002685 Genomic DNA. Translation: AEC09320.1.
AY140105 mRNA. Translation: AAM98246.1.
PIRiA42150.
RefSeqiNP_181228.1. NM_129247.2.
UniGeneiAt.142.

Genome annotation databases

EnsemblPlantsiAT2G36910.1; AT2G36910.1; AT2G36910.
GeneIDi818265.
GrameneiAT2G36910.1; AT2G36910.1; AT2G36910.
KEGGiath:AT2G36910.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61370 Genomic DNA. Translation: CAA43646.1.
AC006922 Genomic DNA. Translation: AAD31576.1.
CP002685 Genomic DNA. Translation: AEC09320.1.
AY140105 mRNA. Translation: AAM98246.1.
PIRiA42150.
RefSeqiNP_181228.1. NM_129247.2.
UniGeneiAt.142.

3D structure databases

ProteinModelPortaliQ9ZR72.
SMRiQ9ZR72. Positions 26-644, 704-1264.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3609. 10 interactions.
IntActiQ9ZR72. 3 interactions.
MINTiMINT-8064482.
STRINGi3702.AT2G36910.1.

Protein family/group databases

TCDBi3.A.1.201.5. the atp-binding cassette (abc) superfamily.

PTM databases

iPTMnetiQ9ZR72.

Proteomic databases

PaxDbiQ9ZR72.
PRIDEiQ9ZR72.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G36910.1; AT2G36910.1; AT2G36910.
GeneIDi818265.
GrameneiAT2G36910.1; AT2G36910.1; AT2G36910.
KEGGiath:AT2G36910.

Organism-specific databases

TAIRiAT2G36910.

Phylogenomic databases

eggNOGiKOG0055. Eukaryota.
COG1132. LUCA.
InParanoidiQ9ZR72.
KOiK05658.
OMAiRISVIMQ.
PhylomeDBiQ9ZR72.

Enzyme and pathway databases

BioCyciARA:AT2G36910-MONOMER.

Miscellaneous databases

PROiQ9ZR72.

Gene expression databases

GenevisibleiQ9ZR72. AT.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure of an mdr-like gene from Arabidopsis thaliana: evolutionary implications."
    Dudler R., Hertig C.
    J. Biol. Chem. 267:5882-5888(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Multidrug resistance-like genes of Arabidopsis required for auxin transport and auxin-mediated development."
    Noh B., Murphy A.S., Spalding E.P.
    Plant Cell 13:2441-2454(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 238-249, FUNCTION, INTERACTION WITH NPA.
  6. "Involvement of an ABC transporter in a developmental pathway regulating hypocotyl cell elongation in the light."
    Sidler M., Hassa P., Hasan S., Ringli C., Dudler R.
    Plant Cell 10:1623-1636(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  7. "A role for ectophosphatase in xenobiotic resistance."
    Thomas C., Rajagopal A., Windsor B., Dudler R., Lloyd A., Roux S.J.
    Plant Cell 12:519-533(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "The Arabidopsis thaliana ABC protein superfamily, a complete inventory."
    Sanchez-Fernandez R., Davies T.G., Coleman J.O., Rea P.A.
    J. Biol. Chem. 276:30231-30244(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  9. "TWISTED DWARF1, a unique plasma membrane-anchored immunophilin-like protein, interacts with Arabidopsis multidrug resistance-like transporters AtPGP1 and AtPGP19."
    Geisler M., Kolukisaoglu H.U., Bouchard R., Billion K., Berger J., Saal B., Frangne N., Koncz-Kalman Z., Koncz C., Dudler R., Blakeslee J.J., Murphy A.S., Martinoia E., Schulz B.
    Mol. Biol. Cell 14:4238-4249(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH FKBP42.
  10. "Multiherbicide tolerance conferred by AtPgp1 and apyrase overexpression in Arabidopsis thaliana."
    Windsor B., Roux S.J., Lloyd A.
    Nat. Biotechnol. 21:428-433(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database."
    Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
    Plant Cell 16:2394-2405(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Two homologous ATP-binding cassette transporter proteins, AtMDR1 and AtPGP1, regulate Arabidopsis photomorphogenesis and root development by mediating polar auxin transport."
    Lin R., Wang H.
    Plant Physiol. 138:949-964(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION, TISSUE SPECIFICITY.
  14. Cited for: GENE FAMILY, NOMENCLATURE.
  15. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAB1B_ARATH
AccessioniPrimary (citable) accession number: Q9ZR72
Secondary accession number(s): Q8L6X6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: May 1, 1999
Last modified: July 6, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.