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Protein

ABC transporter B family member 1

Gene

ABCB1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Auxin efflux transporter that acts as a negative regulator of light signaling to promote hypocotyl elongation. Mediates the accumulation of chlorophyll and anthocyanin, as well as the expression of genes in response to light. Participates directly in auxin efflux and thus regulates the polar (presumably basipetal) auxin transport (from root tips to root elongating zone). Transports also some auxin metabolites such as oxindoleacetic acid and indoleacetaldehyde. Involved in divers auxin-mediated responses including gravitropism, phototropism and lateral root formation. Confers resistance to herbicides such as dicamba, pendimethalin, oryzalin, and monosodium acid methanearsonate (MSMA), but not to herbicides such as glyphosate, atrazine, bentazon and fluazifop-p-butyl. Mediates also resistance to xenobiotics such as cycloheximide and the cytokinin N6-(2-isopentenyl)adenine (2IP).7 Publications

Enzyme regulationi

Transport activity inhibited by 1-N-naphthylphthalamic acid (NPA), cyclopropyl propane dione (CPD), cyclosporin A, verapamil and quercetin.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi403 – 410ATP 1PROSITE-ProRule annotation8
Nucleotide bindingi1059 – 1066ATP 2PROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity, coupled to transmembrane movement of substances Source: TAIR
  • ATP binding Source: UniProtKB-KW
  • auxin efflux transmembrane transporter activity Source: TAIR
  • auxin influx transmembrane transporter activity Source: TAIR

GO - Biological processi

  • anthocyanin accumulation in tissues in response to UV light Source: TAIR
  • auxin-activated signaling pathway Source: UniProtKB-KW
  • auxin efflux Source: TAIR
  • auxin polar transport Source: TAIR
  • photomorphogenesis Source: TAIR
  • positive gravitropism Source: TAIR
  • regulation of cell size Source: TAIR
  • response to auxin Source: TAIR
  • response to blue light Source: TAIR
  • response to nematode Source: TAIR
  • response to red or far red light Source: TAIR
  • stamen development Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Auxin signaling pathway, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G36910-MONOMER.

Protein family/group databases

TCDBi3.A.1.201.5. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
ABC transporter B family member 1
Short name:
ABC transporter ABCB.1
Short name:
AtABCB1
Alternative name(s):
Multidrug resistance protein 1
P-glycoprotein 1
Short name:
AtPgp1
Gene namesi
Name:ABCB1
Synonyms:MDR1, PGP1
Ordered Locus Names:At2g36910
ORF Names:T1J8.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G36910.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei42 – 62HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei93 – 113HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei166 – 186HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei187 – 207HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei277 – 297HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei301 – 321HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei705 – 725HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei745 – 765HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei821 – 843HelicalPROSITE-ProRule annotationAdd BLAST23
Transmembranei845 – 867HelicalPROSITE-ProRule annotationAdd BLAST23
Transmembranei932 – 952HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei967 – 987HelicalPROSITE-ProRule annotationAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: GO_Central
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002279121 – 1286ABC transporter B family member 1Add BLAST1286

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi217N-linked (GlcNAc...)Sequence analysis1
Glycosylationi640N-linked (GlcNAc...)Sequence analysis1
Glycosylationi771N-linked (GlcNAc...)Sequence analysis1
Glycosylationi797N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9ZR72.
PRIDEiQ9ZR72.

PTM databases

iPTMnetiQ9ZR72.

Expressioni

Tissue specificityi

Ubiquitous, with high levels in peduncles. Mostly localized in young developing tissues, including meristems, as well as root and shoot apices.3 Publications

Inductioni

By auxin.1 Publication

Gene expression databases

GenevisibleiQ9ZR72. AT.

Interactioni

Subunit structurei

Interacts with 1-naphthylphthalamic acid (NPA) and FKBP42/TWD1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
FKBP42Q9LDC02EBI-366396,EBI-360006

Protein-protein interaction databases

BioGridi3609. 10 interactors.
IntActiQ9ZR72. 3 interactors.
MINTiMINT-8064482.
STRINGi3702.AT2G36910.1.

Structurei

3D structure databases

ProteinModelPortaliQ9ZR72.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 333ABC transmembrane type-1 1PROSITE-ProRule annotationAdd BLAST290
Domaini368 – 604ABC transporter 1PROSITE-ProRule annotationAdd BLAST237
Domaini700 – 988ABC transmembrane type-1 2PROSITE-ProRule annotationAdd BLAST289
Domaini1024 – 1260ABC transporter 2PROSITE-ProRule annotationAdd BLAST237

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1049 – 1286Interaction with FKBP42/TWD11 PublicationAdd BLAST238

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi8 – 12Poly-Pro5
Compositional biasi621 – 668Ser-richAdd BLAST48

Sequence similaritiesi

Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0055. Eukaryota.
COG1132. LUCA.
InParanoidiQ9ZR72.
KOiK05658.
OMAiGHESTTE.
OrthoDBiEOG093600PE.
PhylomeDBiQ9ZR72.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZR72-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNDGGAPPP PPTLVVEEPK KAEIRGVAFK ELFRFADGLD YVLMGIGSVG
60 70 80 90 100
AFVHGCSLPL FLRFFADLVN SFGSNSNNVE KMMEEVLKYA LYFLVVGAAI
110 120 130 140 150
WASSWAEISC WMWSGERQTT KMRIKYLEAA LNQDIQFFDT EVRTSDVVFA
160 170 180 190 200
INTDAVMVQD AISEKLGNFI HYMATFVSGF IVGFTAVWQL ALVTLAVVPL
210 220 230 240 250
IAVIGGIHTT TLSKLSNKSQ ESLSQAGNIV EQTVVQIRVV MAFVGESRAS
260 270 280 290 300
QAYSSALKIA QKLGYKTGLA KGMGLGATYF VVFCCYALLL WYGGYLVRHH
310 320 330 340 350
LTNGGLAIAT MFAVMIGGLA LGQSAPSMAA FAKAKVAAAK IFRIIDHKPT
360 370 380 390 400
IERNSESGVE LDSVTGLVEL KNVDFSYPSR PDVKILNNFC LSVPAGKTIA
410 420 430 440 450
LVGSSGSGKS TVVSLIERFY DPNSGQVLLD GQDLKTLKLR WLRQQIGLVS
460 470 480 490 500
QEPALFATSI KENILLGRPD ADQVEIEEAA RVANAHSFII KLPDGFDTQV
510 520 530 540 550
GERGLQLSGG QKQRIAIARA MLKNPAILLL DEATSALDSE SEKLVQEALD
560 570 580 590 600
RFMIGRTTLI IAHRLSTIRK ADLVAVLQQG SVSEIGTHDE LFSKGENGVY
610 620 630 640 650
AKLIKMQEAA HETAMSNARK SSARPSSARN SVSSPIMTRN SSYGRSPYSR
660 670 680 690 700
RLSDFSTSDF SLSIDASSYP NYRNEKLAFK DQANSFWRLA KMNSPEWKYA
710 720 730 740 750
LLGSVGSVIC GSLSAFFAYV LSAVLSVYYN PDHEYMIKQI DKYCYLLIGL
760 770 780 790 800
SSAALVFNTL QHSFWDIVGE NLTKRVREKM LSAVLKNEMA WFDQEENESA
810 820 830 840 850
RIAARLALDA NNVRSAIGDR ISVIVQNTAL MLVACTAGFV LQWRLALVLV
860 870 880 890 900
AVFPVVVAAT VLQKMFMTGF SGDLEAAHAK GTQLAGEAIA NVRTVAAFNS
910 920 930 940 950
EAKIVRLYTA NLEPPLKRCF WKGQIAGSGY GVAQFCLYAS YALGLWYASW
960 970 980 990 1000
LVKHGISDFS KTIRVFMVLM VSANGAAETL TLAPDFIKGG QAMRSVFELL
1010 1020 1030 1040 1050
DRKTEIEPDD PDTTPVPDRL RGEVELKHID FSYPSRPDIQ IFRDLSLRAR
1060 1070 1080 1090 1100
AGKTLALVGP SGCGKSSVIS LIQRFYEPSS GRVMIDGKDI RKYNLKAIRK
1110 1120 1130 1140 1150
HIAIVPQEPC LFGTTIYENI AYGHECATEA EIIQAATLAS AHKFISALPE
1160 1170 1180 1190 1200
GYKTYVGERG VQLSGGQKQR IAIARALVRK AEIMLLDEAT SALDAESERS
1210 1220 1230 1240 1250
VQEALDQACS GRTSIVVAHR LSTIRNAHVI AVIDDGKVAE QGSHSHLLKN
1260 1270 1280
HPDGIYARMI QLQRFTHTQV IGMTSGSSSR VKEDDA
Length:1,286
Mass (Da):140,573
Last modified:May 1, 1999 - v1
Checksum:iA6C90AF42CC1FF01
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti293G → D in AAM98246 (PubMed:14593172).Curated1
Sequence conflicti1188E → G in AAM98246 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61370 Genomic DNA. Translation: CAA43646.1.
AC006922 Genomic DNA. Translation: AAD31576.1.
CP002685 Genomic DNA. Translation: AEC09320.1.
AY140105 mRNA. Translation: AAM98246.1.
PIRiA42150.
RefSeqiNP_181228.1. NM_129247.3.
UniGeneiAt.142.

Genome annotation databases

EnsemblPlantsiAT2G36910.1; AT2G36910.1; AT2G36910.
GeneIDi818265.
GrameneiAT2G36910.1; AT2G36910.1; AT2G36910.
KEGGiath:AT2G36910.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61370 Genomic DNA. Translation: CAA43646.1.
AC006922 Genomic DNA. Translation: AAD31576.1.
CP002685 Genomic DNA. Translation: AEC09320.1.
AY140105 mRNA. Translation: AAM98246.1.
PIRiA42150.
RefSeqiNP_181228.1. NM_129247.3.
UniGeneiAt.142.

3D structure databases

ProteinModelPortaliQ9ZR72.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3609. 10 interactors.
IntActiQ9ZR72. 3 interactors.
MINTiMINT-8064482.
STRINGi3702.AT2G36910.1.

Protein family/group databases

TCDBi3.A.1.201.5. the atp-binding cassette (abc) superfamily.

PTM databases

iPTMnetiQ9ZR72.

Proteomic databases

PaxDbiQ9ZR72.
PRIDEiQ9ZR72.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G36910.1; AT2G36910.1; AT2G36910.
GeneIDi818265.
GrameneiAT2G36910.1; AT2G36910.1; AT2G36910.
KEGGiath:AT2G36910.

Organism-specific databases

TAIRiAT2G36910.

Phylogenomic databases

eggNOGiKOG0055. Eukaryota.
COG1132. LUCA.
InParanoidiQ9ZR72.
KOiK05658.
OMAiGHESTTE.
OrthoDBiEOG093600PE.
PhylomeDBiQ9ZR72.

Enzyme and pathway databases

BioCyciARA:AT2G36910-MONOMER.

Miscellaneous databases

PROiQ9ZR72.

Gene expression databases

GenevisibleiQ9ZR72. AT.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAB1B_ARATH
AccessioniPrimary (citable) accession number: Q9ZR72
Secondary accession number(s): Q8L6X6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.