Reviewed, UniProtKB/Swiss-Prot Q9ZR12 (GRH1_ARATH)

Names and origin

Protein names

Recommended name:
    GRR1-like protein 1
Alternative name(s):
    Protein AUXIN SIGNALING F-BOX 1

Gene names

Name:	GRH1
Synonyms:	AFB1, FBL18, GER1, LRF1
Ordered Locus Names:	At4g03190
ORF Names:	F4C21.11

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Auxin receptor that mediates Aux/IAA proteins proteasomal degradation and auxin-regulated transcription. Involved in embryogenesis regulation by auxin. Confers sensitivity to the virulent bacterial pathogen P.syringae. Mediates glucose repression in yeast. Ref.1 Ref.6 Ref.7 Ref.8
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with CUL1, SKP1A/ASK1 and SKP1B/ASK2. Interacts with Aux/IAA proteins (IAA7 and IAA12) in an auxin-dependent manner. Ref.1 Ref.6 Ref.4
Subcellular location	Nucleus. Ref.6
Tissue specificity	Ubiquitous. Ref.6
Induction	Partially repressed by miR393a (microRNA) in response to flg-22 (flagellin-derived peptide 22). Ref.8
Domain	The F-box is necessary for the interaction with SKP1 By similarity.
Sequence similarities	Contains 1 F-box domain.

Ontologies

Keywords
Biological process	Auxin signaling pathway Plant defense Ubl conjugation pathway
Cellular component	Nucleus
Molecular function	Developmental protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	auxin mediated signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB defense response Inferred from electronic annotation. Source: UniProtKB-KW developmental growth Ref.6 Inferred from genetic interaction. Source: TAIR negative regulation of transcription by glucose Ref.1 Traceable author statement. Source: TAIR pollen maturation Inferred from genetic interaction. Source: TAIR response to molecule of bacterial origin Ref.8 Inferred from expression pattern. Source: TAIR stamen development Inferred from genetic interaction. Source: TAIR ubiquitin-dependent protein catabolic process Ref.6 Inferred from genetic interaction. Source: TAIR
Cellular component	nuclear SCF ubiquitin ligase complex Ref.6 Inferred from physical interaction. Source: TAIR
Molecular function	auxin binding Ref.7 Inferred from sequence or structural similarity. Source: UniProtKB inositol hexakisphosphate binding Inferred from sequence or structural similarity. Source: UniProtKB protein binding Ref.1 Ref.6 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
IAA12	Q38830	1	EBI-617479,EBI-617608
IAA7	Q38825	1	EBI-617479,EBI-602959

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 585	585	GRR1-like protein 1		PRO_0000272265
Regions
	Domain	1 – 48	48	F-box
	Region	77 – 78	2	Interaction with auxin-responsive proteins By similarity
	Region	109 – 110	2	Myo-inositol hexakisphosphate binding By similarity
	Region	343 – 348	6	Interaction with auxin-responsive proteins By similarity
	Region	397 – 399	3	Myo-inositol hexakisphosphate binding By similarity
	Region	401 – 405	5	Interaction with auxin-responsive proteins By similarity
	Region	460 – 461	2	Interaction with auxin-responsive proteins By similarity
	Region	480 – 481	2	Myo-inositol hexakisphosphate binding By similarity
Sites
	Binding site	70	1	Myo-inositol hexakisphosphate By similarity
	Binding site	340	1	Myo-inositol hexakisphosphate By similarity
	Binding site	432	1	Myo-inositol hexakisphosphate By similarity
	Binding site	505	1	Myo-inositol hexakisphosphate By similarity
	Site	135	1	Interaction with auxin-responsive proteins By similarity
	Site	161	1	Interaction with auxin-responsive proteins By similarity
	Site	376	1	Interaction with auxin-responsive proteins By similarity
	Site	485	1	Interaction with auxin-responsive proteins By similarity
Experimental info
	Sequence conflict	111	1	M → I in AAK76473. Ref.3
	Sequence conflict	149	1	A → S in AAK01147. Ref.1
	Sequence conflict	293	1	S → T in AAK01147. Ref.1
	Sequence conflict	377	1	C → S in AAK76473. Ref.3
	Sequence conflict	584	1	I → M in AAK76473. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9ZR12-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: D39D627C82864D83	FASTA	585	65,648
	10 20 30 40 50 60 MGLRFPPKVL EHILSFIDSN EDRNSVSLVC KSWFETERKT RKRVFVGNCY AVSPAAVTRR 70 80 90 100 110 120 FPEMRSLTLK GKPHFADYNL VPDGWGGYAW PWIEAMAAKS SSLEEIRMKR MVVTDECLEK 130 140 150 160 170 180 IAASFKDFKV LVLTSCEGFS TDGIAAIAAT CRNLRVLELR ECIVEDLGGD WLSYFPESST 190 200 210 220 230 240 SLVSLDFSCL DSEVKISDLE RLVSRSPNLK SLKLNPAVTL DGLVSLLRCA PQLTELGTGS 250 260 270 280 290 300 FAAQLKPEAF SKLSEAFSNC KQLQSLSGLW DVLPEYLPAL YSVCPGLTSL NLSYATVRMP 310 320 330 340 350 360 DLVELLRRCS KLQKLWVMDL IEDKGLEAVA SYCKELRELR VFPSEPDLDA TNIPLTEQGL 370 380 390 400 410 420 VFVSKGCRKL ESVLYFCVQF TNAALFTIAR KRPNLKCFRL CVIEPFAPDY KTNEPLDKGF 430 440 450 460 470 480 KAIAEGCRDL RRLSVSGLLS DKAFKYIGKH AKKVRMLSIA FAGDSDLMLH HLLSGCESLK 490 500 510 520 530 540 KLEIRDCPFG DTALLEHAAK LETMRSLWMS SCFVSFGACK LLSQKMPRLN VEVIDEHPPE 550 560 570 580 SRPESSPVER IYIYRTVAGP RMDTPEFVWT IHKNPENGVS HLAIK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning by pathway activation in yeast: identification of an Arabidopsis thaliana F-box protein that can turn on glucose repression." Thelander M., Fredriksson D., Schouten J., Hoge J.H.C., Ronne H. Plant Mol. Biol. 49:69-79(2002) [PubMed: 12008900] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH SKP1A/ASK1 AND SKP1B/ASK2, LEUCINE-RICH REPEATS.
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Identification of an SCF ubiquitin-ligase complex required for auxin response in Arabidopsis thaliana." Gray W.M., del Pozo J.C., Walker L., Hobbie L., Risseeuw E., Banks T., Crosby W.L., Yang M., Ma H., Estelle M. Genes Dev. 13:1678-1691(1999) [PubMed: 10398681] [Abstract] Cited for: INTERACTION WITH SKP1A/ASK1 AND SKP1B/ASK2.
[5]	"F-box proteins in Arabidopsis." Xiao W., Jang J.-C. Trends Plant Sci. 5:454-457(2000) [PubMed: 11077244] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[6]	"Plant development is regulated by a family of auxin receptor F box proteins." Dharmasiri N., Dharmasiri S., Weijers D., Lechner E., Yamada M., Hobbie L., Ehrismann J.S., Juergens G., Estelle M. Dev. Cell 9:109-119(2005) [PubMed: 15992545] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH CUL1; IAA7; IAA12 AND SKP1A/ASK1.
[7]	"The F-box protein TIR1 is an auxin receptor." Dharmasiri N., Dharmasiri S., Estelle M. Nature 435:441-445(2005) [PubMed: 15917797] [Abstract] Cited for: FUNCTION.
[8]	"A plant miRNA contributes to antibacterial resistance by repressing auxin signaling." Navarro L., Dunoyer P., Jay F., Arnold B., Dharmasiri N., Estelle M., Voinnet O., Jones J.D.G. Science 312:436-439(2006) [PubMed: 16627744] [Abstract] Cited for: FUNCTION, INDUCTION.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF291816 mRNA. Translation: AAK01147.1. AC005275 Genomic DNA. Translation: AAD14447.1. AL161496 Genomic DNA. Translation: CAB77804.1. AY045799 mRNA. Translation: AAK76473.1. AY150427 mRNA. Translation: AAN12969.1.
IPI	IPI00536129.
PIR	E85040.
RefSeq	NP_567255.1.
UniGene	At.24291
3D structure databases
SMR	Q9ZR12. Positions 6-571.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9ZR12. 5 interactions.
Proteomic databases
PRIDE	Q9ZR12.
Genome annotation databases
GeneID	828045.
GenomeReviews	Gene locus AT4G03190 in contig CT486007_GR.
KEGG	ath:AT4G03190.
NMPDR	fig|3702.1.peg.18146.
Organism-specific databases
TAIR	At4g03190.
Family and domain databases
InterPro	IPR001810. F-box. [Graphical view]
SMART	SM00256. FBOX. 1 hit. [Graphical view]
PROSITE	PS50181. FBOX. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GRH1_ARATH

Accession

Primary (citable) accession number: Q9ZR12
Secondary accession number(s): Q94AU0, Q9C5Y7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)