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Q9ZR12

- GRH1_ARATH

UniProt

Q9ZR12 - GRH1_ARATH

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Protein
GRR1-like protein 1
Gene
GRH1, AFB1, FBL18, GER1, LRF1, At4g03190, F4C21.11
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Auxin receptor that mediates Aux/IAA proteins proteasomal degradation and auxin-regulated transcription. Involved in embryogenesis regulation by auxin. Confers sensitivity to the virulent bacterial pathogen P.syringae. Mediates glucose repression in yeast.4 Publications

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei70 – 701Myo-inositol hexakisphosphate By similarity
Sitei135 – 1351Interaction with auxin-responsive proteins By similarity
Sitei161 – 1611Interaction with auxin-responsive proteins By similarity
Binding sitei340 – 3401Myo-inositol hexakisphosphate By similarity
Sitei376 – 3761Interaction with auxin-responsive proteins By similarity
Binding sitei432 – 4321Myo-inositol hexakisphosphate By similarity
Sitei485 – 4851Interaction with auxin-responsive proteins By similarity
Binding sitei505 – 5051Myo-inositol hexakisphosphate By similarity

GO - Molecular functioni

  1. auxin binding Source: UniProtKB
  2. inositol hexakisphosphate binding Source: UniProtKB
  3. protein binding Source: IntAct
  4. ubiquitin-protein transferase activity Source: TAIR

GO - Biological processi

  1. auxin-activated signaling pathway Source: UniProtKB
  2. defense response Source: UniProtKB-KW
  3. negative regulation of transcription by glucose Source: TAIR
  4. pollen maturation Source: TAIR
  5. response to auxin Source: TAIR
  6. response to molecule of bacterial origin Source: TAIR
  7. stamen development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Auxin signaling pathway, Plant defense, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
GRR1-like protein 1
Alternative name(s):
Protein AUXIN SIGNALING F-BOX 1
Gene namesi
Name:GRH1
Synonyms:AFB1, FBL18, GER1, LRF1
Ordered Locus Names:At4g03190
ORF Names:F4C21.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G03190.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. SCF ubiquitin ligase complex Source: UniProtKB
  2. nuclear SCF ubiquitin ligase complex Source: TAIR
  3. nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 585585GRR1-like protein 1
PRO_0000272265Add
BLAST

Proteomic databases

PaxDbiQ9ZR12.
PRIDEiQ9ZR12.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Inductioni

Partially repressed by miR393a (microRNA) in response to flg-22 (flagellin-derived peptide 22).1 Publication

Gene expression databases

ArrayExpressiQ9ZR12.
GenevestigatoriQ9ZR12.

Interactioni

Subunit structurei

Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with CUL1, SKP1A/ASK1 and SKP1B/ASK2. Interacts with Aux/IAA proteins (IAA7 and IAA12) in an auxin-dependent manner.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SKP1AQ392553EBI-617479,EBI-532357

Protein-protein interaction databases

BioGridi13336. 9 interactions.
DIPiDIP-34607N.
IntActiQ9ZR12. 6 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9ZR12.
SMRiQ9ZR12. Positions 6-571.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 4848F-box
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni77 – 782Interaction with auxin-responsive proteins By similarity
Regioni109 – 1102Myo-inositol hexakisphosphate binding By similarity
Regioni343 – 3486Interaction with auxin-responsive proteins By similarity
Regioni397 – 3993Myo-inositol hexakisphosphate binding By similarity
Regioni401 – 4055Interaction with auxin-responsive proteins By similarity
Regioni460 – 4612Interaction with auxin-responsive proteins By similarity
Regioni480 – 4812Myo-inositol hexakisphosphate binding By similarity

Domaini

The F-box is necessary for the interaction with SKP1 By similarity.

Sequence similaritiesi

Contains 1 F-box domain.

Phylogenomic databases

eggNOGiNOG324137.
HOGENOMiHOG000239805.
InParanoidiQ9ZR12.
OMAiSWFETER.
PhylomeDBiQ9ZR12.

Family and domain databases

InterProiIPR001810. F-box_dom.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZR12-1 [UniParc]FASTAAdd to Basket

« Hide

MGLRFPPKVL EHILSFIDSN EDRNSVSLVC KSWFETERKT RKRVFVGNCY    50
AVSPAAVTRR FPEMRSLTLK GKPHFADYNL VPDGWGGYAW PWIEAMAAKS 100
SSLEEIRMKR MVVTDECLEK IAASFKDFKV LVLTSCEGFS TDGIAAIAAT 150
CRNLRVLELR ECIVEDLGGD WLSYFPESST SLVSLDFSCL DSEVKISDLE 200
RLVSRSPNLK SLKLNPAVTL DGLVSLLRCA PQLTELGTGS FAAQLKPEAF 250
SKLSEAFSNC KQLQSLSGLW DVLPEYLPAL YSVCPGLTSL NLSYATVRMP 300
DLVELLRRCS KLQKLWVMDL IEDKGLEAVA SYCKELRELR VFPSEPDLDA 350
TNIPLTEQGL VFVSKGCRKL ESVLYFCVQF TNAALFTIAR KRPNLKCFRL 400
CVIEPFAPDY KTNEPLDKGF KAIAEGCRDL RRLSVSGLLS DKAFKYIGKH 450
AKKVRMLSIA FAGDSDLMLH HLLSGCESLK KLEIRDCPFG DTALLEHAAK 500
LETMRSLWMS SCFVSFGACK LLSQKMPRLN VEVIDEHPPE SRPESSPVER 550
IYIYRTVAGP RMDTPEFVWT IHKNPENGVS HLAIK 585
Length:585
Mass (Da):65,648
Last modified:May 1, 1999 - v1
Checksum:iD39D627C82864D83
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti111 – 1111M → I in AAK76473. 1 Publication
Sequence conflicti149 – 1491A → S in AAK01147. 1 Publication
Sequence conflicti293 – 2931S → T in AAK01147. 1 Publication
Sequence conflicti377 – 3771C → S in AAK76473. 1 Publication
Sequence conflicti584 – 5841I → M in AAK76473. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF291816 mRNA. Translation: AAK01147.1.
AC005275 Genomic DNA. Translation: AAD14447.1.
AL161496 Genomic DNA. Translation: CAB77804.1.
CP002687 Genomic DNA. Translation: AEE82287.1.
AY045799 mRNA. Translation: AAK76473.1.
AY150427 mRNA. Translation: AAN12969.1.
PIRiE85040.
RefSeqiNP_567255.1. NM_116555.3.
UniGeneiAt.24291.
At.69891.

Genome annotation databases

EnsemblPlantsiAT4G03190.1; AT4G03190.1; AT4G03190.
GeneIDi828045.
KEGGiath:AT4G03190.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF291816 mRNA. Translation: AAK01147.1 .
AC005275 Genomic DNA. Translation: AAD14447.1 .
AL161496 Genomic DNA. Translation: CAB77804.1 .
CP002687 Genomic DNA. Translation: AEE82287.1 .
AY045799 mRNA. Translation: AAK76473.1 .
AY150427 mRNA. Translation: AAN12969.1 .
PIRi E85040.
RefSeqi NP_567255.1. NM_116555.3.
UniGenei At.24291.
At.69891.

3D structure databases

ProteinModelPortali Q9ZR12.
SMRi Q9ZR12. Positions 6-571.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 13336. 9 interactions.
DIPi DIP-34607N.
IntActi Q9ZR12. 6 interactions.

Proteomic databases

PaxDbi Q9ZR12.
PRIDEi Q9ZR12.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G03190.1 ; AT4G03190.1 ; AT4G03190 .
GeneIDi 828045.
KEGGi ath:AT4G03190.

Organism-specific databases

TAIRi AT4G03190.

Phylogenomic databases

eggNOGi NOG324137.
HOGENOMi HOG000239805.
InParanoidi Q9ZR12.
OMAi SWFETER.
PhylomeDBi Q9ZR12.

Enzyme and pathway databases

UniPathwayi UPA00143 .

Gene expression databases

ArrayExpressi Q9ZR12.
Genevestigatori Q9ZR12.

Family and domain databases

InterProi IPR001810. F-box_dom.
[Graphical view ]
SMARTi SM00256. FBOX. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning by pathway activation in yeast: identification of an Arabidopsis thaliana F-box protein that can turn on glucose repression."
    Thelander M., Fredriksson D., Schouten J., Hoge J.H.C., Ronne H.
    Plant Mol. Biol. 49:69-79(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH SKP1A/ASK1 AND SKP1B/ASK2, LEUCINE-RICH REPEATS.
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Identification of an SCF ubiquitin-ligase complex required for auxin response in Arabidopsis thaliana."
    Gray W.M., del Pozo J.C., Walker L., Hobbie L., Risseeuw E., Banks T., Crosby W.L., Yang M., Ma H., Estelle M.
    Genes Dev. 13:1678-1691(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SKP1A/ASK1 AND SKP1B/ASK2.
  6. Cited for: GENE FAMILY, NOMENCLATURE.
  7. "Plant development is regulated by a family of auxin receptor F box proteins."
    Dharmasiri N., Dharmasiri S., Weijers D., Lechner E., Yamada M., Hobbie L., Ehrismann J.S., Juergens G., Estelle M.
    Dev. Cell 9:109-119(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH CUL1; IAA7; IAA12 AND SKP1A/ASK1.
  8. "The F-box protein TIR1 is an auxin receptor."
    Dharmasiri N., Dharmasiri S., Estelle M.
    Nature 435:441-445(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "A plant miRNA contributes to antibacterial resistance by repressing auxin signaling."
    Navarro L., Dunoyer P., Jay F., Arnold B., Dharmasiri N., Estelle M., Voinnet O., Jones J.D.G.
    Science 312:436-439(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.

Entry informationi

Entry nameiGRH1_ARATH
AccessioniPrimary (citable) accession number: Q9ZR12
Secondary accession number(s): Q94AU0, Q9C5Y7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: May 1, 1999
Last modified: September 3, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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