Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9ZR12

- GRH1_ARATH

UniProt

Q9ZR12 - GRH1_ARATH

Protein

GRR1-like protein 1

Gene

GRH1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Auxin receptor that mediates Aux/IAA proteins proteasomal degradation and auxin-regulated transcription. Involved in embryogenesis regulation by auxin. Confers sensitivity to the virulent bacterial pathogen P.syringae. Mediates glucose repression in yeast.4 Publications

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei70 – 701Myo-inositol hexakisphosphateBy similarity
    Sitei135 – 1351Interaction with auxin-responsive proteinsBy similarity
    Sitei161 – 1611Interaction with auxin-responsive proteinsBy similarity
    Binding sitei340 – 3401Myo-inositol hexakisphosphateBy similarity
    Sitei376 – 3761Interaction with auxin-responsive proteinsBy similarity
    Binding sitei432 – 4321Myo-inositol hexakisphosphateBy similarity
    Sitei485 – 4851Interaction with auxin-responsive proteinsBy similarity
    Binding sitei505 – 5051Myo-inositol hexakisphosphateBy similarity

    GO - Molecular functioni

    1. auxin binding Source: UniProtKB
    2. inositol hexakisphosphate binding Source: UniProtKB
    3. protein binding Source: IntAct
    4. ubiquitin-protein transferase activity Source: TAIR

    GO - Biological processi

    1. auxin-activated signaling pathway Source: UniProtKB
    2. defense response Source: UniProtKB-KW
    3. negative regulation of transcription by glucose Source: TAIR
    4. pollen maturation Source: TAIR
    5. response to auxin Source: TAIR
    6. response to molecule of bacterial origin Source: TAIR
    7. stamen development Source: TAIR

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Auxin signaling pathway, Plant defense, Ubl conjugation pathway

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GRR1-like protein 1
    Alternative name(s):
    Protein AUXIN SIGNALING F-BOX 1
    Gene namesi
    Name:GRH1
    Synonyms:AFB1, FBL18, GER1, LRF1
    Ordered Locus Names:At4g03190
    ORF Names:F4C21.11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G03190.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nuclear SCF ubiquitin ligase complex Source: TAIR
    2. nucleus Source: TAIR
    3. SCF ubiquitin ligase complex Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 585585GRR1-like protein 1PRO_0000272265Add
    BLAST

    Proteomic databases

    PaxDbiQ9ZR12.
    PRIDEiQ9ZR12.

    Expressioni

    Tissue specificityi

    Ubiquitous.1 Publication

    Inductioni

    Partially repressed by miR393a (microRNA) in response to flg-22 (flagellin-derived peptide 22).1 Publication

    Gene expression databases

    ArrayExpressiQ9ZR12.
    GenevestigatoriQ9ZR12.

    Interactioni

    Subunit structurei

    Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with CUL1, SKP1A/ASK1 and SKP1B/ASK2. Interacts with Aux/IAA proteins (IAA7 and IAA12) in an auxin-dependent manner.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SKP1AQ392553EBI-617479,EBI-532357

    Protein-protein interaction databases

    BioGridi13336. 9 interactions.
    DIPiDIP-34607N.
    IntActiQ9ZR12. 6 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ZR12.
    SMRiQ9ZR12. Positions 6-571.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 4848F-boxAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni77 – 782Interaction with auxin-responsive proteinsBy similarity
    Regioni109 – 1102Myo-inositol hexakisphosphate bindingBy similarity
    Regioni343 – 3486Interaction with auxin-responsive proteinsBy similarity
    Regioni397 – 3993Myo-inositol hexakisphosphate bindingBy similarity
    Regioni401 – 4055Interaction with auxin-responsive proteinsBy similarity
    Regioni460 – 4612Interaction with auxin-responsive proteinsBy similarity
    Regioni480 – 4812Myo-inositol hexakisphosphate bindingBy similarity

    Domaini

    The F-box is necessary for the interaction with SKP1.By similarity

    Sequence similaritiesi

    Contains 1 F-box domain.Curated

    Phylogenomic databases

    eggNOGiNOG324137.
    HOGENOMiHOG000239805.
    InParanoidiQ9ZR12.
    OMAiSWFETER.
    PhylomeDBiQ9ZR12.

    Family and domain databases

    InterProiIPR001810. F-box_dom.
    [Graphical view]
    SMARTiSM00256. FBOX. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9ZR12-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLRFPPKVL EHILSFIDSN EDRNSVSLVC KSWFETERKT RKRVFVGNCY    50
    AVSPAAVTRR FPEMRSLTLK GKPHFADYNL VPDGWGGYAW PWIEAMAAKS 100
    SSLEEIRMKR MVVTDECLEK IAASFKDFKV LVLTSCEGFS TDGIAAIAAT 150
    CRNLRVLELR ECIVEDLGGD WLSYFPESST SLVSLDFSCL DSEVKISDLE 200
    RLVSRSPNLK SLKLNPAVTL DGLVSLLRCA PQLTELGTGS FAAQLKPEAF 250
    SKLSEAFSNC KQLQSLSGLW DVLPEYLPAL YSVCPGLTSL NLSYATVRMP 300
    DLVELLRRCS KLQKLWVMDL IEDKGLEAVA SYCKELRELR VFPSEPDLDA 350
    TNIPLTEQGL VFVSKGCRKL ESVLYFCVQF TNAALFTIAR KRPNLKCFRL 400
    CVIEPFAPDY KTNEPLDKGF KAIAEGCRDL RRLSVSGLLS DKAFKYIGKH 450
    AKKVRMLSIA FAGDSDLMLH HLLSGCESLK KLEIRDCPFG DTALLEHAAK 500
    LETMRSLWMS SCFVSFGACK LLSQKMPRLN VEVIDEHPPE SRPESSPVER 550
    IYIYRTVAGP RMDTPEFVWT IHKNPENGVS HLAIK 585
    Length:585
    Mass (Da):65,648
    Last modified:May 1, 1999 - v1
    Checksum:iD39D627C82864D83
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti111 – 1111M → I in AAK76473. (PubMed:14593172)Curated
    Sequence conflicti149 – 1491A → S in AAK01147. (PubMed:12008900)Curated
    Sequence conflicti293 – 2931S → T in AAK01147. (PubMed:12008900)Curated
    Sequence conflicti377 – 3771C → S in AAK76473. (PubMed:14593172)Curated
    Sequence conflicti584 – 5841I → M in AAK76473. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF291816 mRNA. Translation: AAK01147.1.
    AC005275 Genomic DNA. Translation: AAD14447.1.
    AL161496 Genomic DNA. Translation: CAB77804.1.
    CP002687 Genomic DNA. Translation: AEE82287.1.
    AY045799 mRNA. Translation: AAK76473.1.
    AY150427 mRNA. Translation: AAN12969.1.
    PIRiE85040.
    RefSeqiNP_567255.1. NM_116555.3.
    UniGeneiAt.24291.
    At.69891.

    Genome annotation databases

    EnsemblPlantsiAT4G03190.1; AT4G03190.1; AT4G03190.
    GeneIDi828045.
    KEGGiath:AT4G03190.

    Cross-referencesi

    Web resourcesi

    PlantsUBQ

    A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF291816 mRNA. Translation: AAK01147.1 .
    AC005275 Genomic DNA. Translation: AAD14447.1 .
    AL161496 Genomic DNA. Translation: CAB77804.1 .
    CP002687 Genomic DNA. Translation: AEE82287.1 .
    AY045799 mRNA. Translation: AAK76473.1 .
    AY150427 mRNA. Translation: AAN12969.1 .
    PIRi E85040.
    RefSeqi NP_567255.1. NM_116555.3.
    UniGenei At.24291.
    At.69891.

    3D structure databases

    ProteinModelPortali Q9ZR12.
    SMRi Q9ZR12. Positions 6-571.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 13336. 9 interactions.
    DIPi DIP-34607N.
    IntActi Q9ZR12. 6 interactions.

    Proteomic databases

    PaxDbi Q9ZR12.
    PRIDEi Q9ZR12.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G03190.1 ; AT4G03190.1 ; AT4G03190 .
    GeneIDi 828045.
    KEGGi ath:AT4G03190.

    Organism-specific databases

    TAIRi AT4G03190.

    Phylogenomic databases

    eggNOGi NOG324137.
    HOGENOMi HOG000239805.
    InParanoidi Q9ZR12.
    OMAi SWFETER.
    PhylomeDBi Q9ZR12.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Gene expression databases

    ArrayExpressi Q9ZR12.
    Genevestigatori Q9ZR12.

    Family and domain databases

    InterProi IPR001810. F-box_dom.
    [Graphical view ]
    SMARTi SM00256. FBOX. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning by pathway activation in yeast: identification of an Arabidopsis thaliana F-box protein that can turn on glucose repression."
      Thelander M., Fredriksson D., Schouten J., Hoge J.H.C., Ronne H.
      Plant Mol. Biol. 49:69-79(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH SKP1A/ASK1 AND SKP1B/ASK2, LEUCINE-RICH REPEATS.
    2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Identification of an SCF ubiquitin-ligase complex required for auxin response in Arabidopsis thaliana."
      Gray W.M., del Pozo J.C., Walker L., Hobbie L., Risseeuw E., Banks T., Crosby W.L., Yang M., Ma H., Estelle M.
      Genes Dev. 13:1678-1691(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SKP1A/ASK1 AND SKP1B/ASK2.
    6. Cited for: GENE FAMILY, NOMENCLATURE.
    7. "Plant development is regulated by a family of auxin receptor F box proteins."
      Dharmasiri N., Dharmasiri S., Weijers D., Lechner E., Yamada M., Hobbie L., Ehrismann J.S., Juergens G., Estelle M.
      Dev. Cell 9:109-119(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH CUL1; IAA7; IAA12 AND SKP1A/ASK1.
    8. "The F-box protein TIR1 is an auxin receptor."
      Dharmasiri N., Dharmasiri S., Estelle M.
      Nature 435:441-445(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "A plant miRNA contributes to antibacterial resistance by repressing auxin signaling."
      Navarro L., Dunoyer P., Jay F., Arnold B., Dharmasiri N., Estelle M., Voinnet O., Jones J.D.G.
      Science 312:436-439(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.

    Entry informationi

    Entry nameiGRH1_ARATH
    AccessioniPrimary (citable) accession number: Q9ZR12
    Secondary accession number(s): Q94AU0, Q9C5Y7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2007
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3