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Protein

Cytochrome b6-f complex iron-sulfur subunit, chloroplastic

Gene

petC

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential protein for photoautotrophism. Confers resistance to photo-oxidative damages by contributing to the thermal dissipation of light energy and to lumenal acidification (increase of pH gradient). Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions (By similarity).By similarity3 Publications

Catalytic activityi

Plastoquinol + 2 oxidized plastocyanin + 2 H+(Side 1) = plastoquinone + 2 reduced plastocyanin + 4 H+(Side 2).

Cofactori

[2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi157 – 1571Iron-sulfur (2Fe-2S)By similarity
Metal bindingi159 – 1591Iron-sulfur (2Fe-2S); via pros nitrogenBy similarity
Metal bindingi175 – 1751Iron-sulfur (2Fe-2S)By similarity
Metal bindingi178 – 1781Iron-sulfur (2Fe-2S); via pros nitrogenBy similarity

GO - Molecular functioni

GO - Biological processi

  • defense response to bacterium Source: TAIR
  • nonphotochemical quenching Source: TAIR
  • photosynthetic electron transport chain Source: TAIR
  • response to karrikin Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT4G03280-MONOMER.
ARA:GQT-2516-MONOMER.

Protein family/group databases

TCDBi3.D.3.5.2. the proton-translocating quinol:cytochrome c reductase (qcr) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b6-f complex iron-sulfur subunit, chloroplastic (EC:1.10.9.1)
Alternative name(s):
Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein
Proton gradient regulation protein 1
Rieske iron-sulfur protein
Short name:
ISP
Short name:
RISP
Gene namesi
Name:petC
Synonyms:PGR1
Ordered Locus Names:At4g03280
ORF Names:F4C21.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G03280.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei68 – 9023HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast thylakoid Source: TAIR
  • chloroplast thylakoid membrane Source: TAIR
  • cytochrome b6f complex Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: TAIR
  • plasma membrane Source: TAIR
  • thylakoid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi194 – 1941P → L in pgr1; reduces electron transfer from cyt b6/f to photosystem I at high light intensity, and shifts pH-dependent inactivation of electron transport to more alkaline pH. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5050ChloroplastSequence analysisAdd
BLAST
Chaini51 – 229179Cytochrome b6-f complex iron-sulfur subunit, chloroplasticPRO_0000030684Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi162 ↔ 177By similarity
Modified residuei196 – 1961PhosphoserineCombined sources

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiQ9ZR03.
PRIDEiQ9ZR03.

PTM databases

iPTMnetiQ9ZR03.

Expressioni

Tissue specificityi

Confined to photosynthetic tissues, with highest levels in flowers. In leaves, mostly localized in mesophyll cells. In stems, confined to the peripheral ring of chlorenchyma and adjoining groups of cells associated with the vascular bundles. In siliques, present in green wall of the fruit and in peduncle but not in the translucide white septum of the seeds.1 Publication

Inductioni

Light-dependent expression. Inhibited by acidification of thylakoids (below pH 5), sucrose and norflurazon (caroteonid synthesis inhibitor leading to photobleaching).2 Publications

Gene expression databases

GenevisibleiQ9ZR03. AT.

Interactioni

Subunit structurei

The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer (By similarity). Interacts with PGRL1A.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PNSL4Q9SCY33EBI-2436968,EBI-2436954

Protein-protein interaction databases

BioGridi13287. 4 interactions.
IntActiQ9ZR03. 3 interactions.
STRINGi3702.AT4G03280.1.

Structurei

3D structure databases

ProteinModelPortaliQ9ZR03.
SMRiQ9ZR03. Positions 59-229.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini115 – 21197RieskeAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi68 – 736Poly-Leu
Compositional biasi95 – 1028Poly-Gly

Sequence similaritiesi

Contains 1 Rieske domain.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1671. Eukaryota.
COG0723. LUCA.
HOGENOMiHOG000255192.
InParanoidiQ9ZR03.
KOiK02636.
OMAiKGDPTYI.
OrthoDBiEOG09360NVS.
PhylomeDBiQ9ZR03.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
HAMAPiMF_01335. Cytb6_f_Rieske. 1 hit.
InterProiIPR014909. Cyt_b6-f_cplx_Fe-S_su.
IPR023960. Cyt_b6_f_Rieske.
IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
[Graphical view]
PANTHERiPTHR10134. PTHR10134. 1 hit.
PfamiPF08802. CytB6-F_Fe-S. 1 hit.
PF00355. Rieske. 1 hit.
[Graphical view]
PRINTSiPR00162. RIESKE.
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9ZR03-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSSLSPAT QLGSSRSALM AMSSGLFVKP TKMNHQMVRK EKIGLRISCQ
60 70 80 90 100
ASSIPADRVP DMEKRKTLNL LLLGALSLPT GYMLVPYATF FVPPGTGGGG
110 120 130 140 150
GGTPAKDALG NDVVAAEWLK THGPGDRTLT QGLKGDPTYL VVENDKTLAT
160 170 180 190 200
YGINAVCTHL GCVVPWNKAE NKFLCPCHGS QYNAQGRVVR GPAPLSLALA
210 220
HADIDEAGKV LFVPWVETDF RTGDAPWWS
Length:229
Mass (Da):24,366
Last modified:May 1, 1999 - v1
Checksum:iD79FAE03D85EA2F5
GO
Isoform 2 (identifier: Q9ZR03-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: Missing.

Note: May be due to an intron retention. No experimental confirmation available.
Show »
Length:210
Mass (Da):22,533
Checksum:iD4A249FC478EC230
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti48 – 481S → A in CAC03598 (Ref. 2) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1919Missing in isoform 2. 1 PublicationVSP_015096Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ292972 Genomic DNA. Translation: CAC03598.1.
AJ243702 mRNA. Translation: CAB52433.1.
AC005275 Genomic DNA. Translation: AAD14456.1.
AL161496 Genomic DNA. Translation: CAB77813.1.
CP002687 Genomic DNA. Translation: AEE82301.1.
CP002687 Genomic DNA. Translation: AEE82302.1.
AF370566 mRNA. Translation: AAK49572.1.
AF410296 mRNA. Translation: AAK95282.1.
AY093726 mRNA. Translation: AAM10350.1.
PIRiF85041.
PA0041.
RefSeqiNP_192237.1. NM_116566.4. [Q9ZR03-1]
NP_849295.1. NM_178964.2. [Q9ZR03-2]
UniGeneiAt.486.
At.67366.

Genome annotation databases

EnsemblPlantsiAT4G03280.1; AT4G03280.1; AT4G03280. [Q9ZR03-1]
GeneIDi827996.
KEGGiath:AT4G03280.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ292972 Genomic DNA. Translation: CAC03598.1.
AJ243702 mRNA. Translation: CAB52433.1.
AC005275 Genomic DNA. Translation: AAD14456.1.
AL161496 Genomic DNA. Translation: CAB77813.1.
CP002687 Genomic DNA. Translation: AEE82301.1.
CP002687 Genomic DNA. Translation: AEE82302.1.
AF370566 mRNA. Translation: AAK49572.1.
AF410296 mRNA. Translation: AAK95282.1.
AY093726 mRNA. Translation: AAM10350.1.
PIRiF85041.
PA0041.
RefSeqiNP_192237.1. NM_116566.4. [Q9ZR03-1]
NP_849295.1. NM_178964.2. [Q9ZR03-2]
UniGeneiAt.486.
At.67366.

3D structure databases

ProteinModelPortaliQ9ZR03.
SMRiQ9ZR03. Positions 59-229.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi13287. 4 interactions.
IntActiQ9ZR03. 3 interactions.
STRINGi3702.AT4G03280.1.

Protein family/group databases

TCDBi3.D.3.5.2. the proton-translocating quinol:cytochrome c reductase (qcr) superfamily.

PTM databases

iPTMnetiQ9ZR03.

Proteomic databases

PaxDbiQ9ZR03.
PRIDEiQ9ZR03.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G03280.1; AT4G03280.1; AT4G03280. [Q9ZR03-1]
GeneIDi827996.
KEGGiath:AT4G03280.

Organism-specific databases

TAIRiAT4G03280.

Phylogenomic databases

eggNOGiKOG1671. Eukaryota.
COG0723. LUCA.
HOGENOMiHOG000255192.
InParanoidiQ9ZR03.
KOiK02636.
OMAiKGDPTYI.
OrthoDBiEOG09360NVS.
PhylomeDBiQ9ZR03.

Enzyme and pathway databases

BioCyciARA:AT4G03280-MONOMER.
ARA:GQT-2516-MONOMER.

Miscellaneous databases

PROiQ9ZR03.

Gene expression databases

GenevisibleiQ9ZR03. AT.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
HAMAPiMF_01335. Cytb6_f_Rieske. 1 hit.
InterProiIPR014909. Cyt_b6-f_cplx_Fe-S_su.
IPR023960. Cyt_b6_f_Rieske.
IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
[Graphical view]
PANTHERiPTHR10134. PTHR10134. 1 hit.
PfamiPF08802. CytB6-F_Fe-S. 1 hit.
PF00355. Rieske. 1 hit.
[Graphical view]
PRINTSiPR00162. RIESKE.
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUCRIA_ARATH
AccessioniPrimary (citable) accession number: Q9ZR03
Secondary accession number(s): Q94EI4, Q9FYB6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: May 1, 1999
Last modified: September 7, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein is 1 of 2 subunits of the cytochrome b6-f complex that are encoded in the nucleus.
The Rieske iron-sulfur protein is a high potential 2Fe-2S protein.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.