Q9ZQP1 (DSP8_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative dual specificity protein phosphatase DSP8
EC=3.1.3.16
EC=3.1.3.48

Gene names

Name:	DSP8
Ordered Locus Names:	At2g35680
ORF Names:	T20F21.13

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May possess phosphatase activity By similarity.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate. Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Sequence similarities	Contains 1 tyrosine-protein phosphatase domain.
Sequence caution	The sequence AAK96749.1 differs from that shown. Reason: Frameshift at position 239.

Ontologies

Keywords
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	inositol phosphate dephosphorylation Inferred from Biological aspect of Ancestor. Source: RefGenome peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC
Molecular_function	phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity Inferred from Biological aspect of Ancestor. Source: RefGenome protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC protein tyrosine/serine/threonine phosphatase activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Putative dual specificity protein phosphatase DSP8		PRO_0000417332
Regions
	Domain	73 – 219	147	Tyrosine-protein phosphatase
	Region	164 – 170	7	Substrate binding By similarity
Sites
	Active site	164	1	Phosphocysteine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZQP1 [UniParc]. Last modified June 1, 2002. Version 2. Checksum: 8A11D39F2772D2A2	FASTA	337	37,548
	10 20 30 40 50 60 MYIKELTETD EEKRERSVED NVDDGDKAVL VSRGNVIVLT TKRALVGVGA RALFYPTLVY 70 80 90 100 110 120 NVVRNKLESE FRWWDRVAEF ILLGAVPFPS DVPQLKELGV CGVITLNEPY ETLVPSSLYK 130 140 150 160 170 180 SYCIDHLVIA TRDYCFAPSM EAICQAVEFI HRNASLGKTT YVHCKAGRGR STTIVICYLV 190 200 210 220 230 240 QHKNMTPEAA YSYVRSIRPR VLLAAAQWKA VVEYYHVKVL NTQSCLTDAT SALIPRNVKQ 250 260 270 280 290 300 VCSGNVVVFD DGSMVVVTHS DLEGYNDDDS RSRRSVKVNG NELWAAAADL SMVYRVKVVG 310 320 330 QAAMARISCL WLGLREDQKL SGKNLSMGGI SVDISVY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A stress inducible dual-specific phosphatase that plays a role in ABA and sugar signaling." Gupta R., Sokolov L.N., Luan S. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ574761 mRNA. Translation: CAE00415.1. AC006068 Genomic DNA. Translation: AAD15447.2. CP002685 Genomic DNA. Translation: AEC09144.1. AY054558 mRNA. Translation: AAK96749.1. Frameshift. AY065219 mRNA. Translation: AAL38695.1. BT008574 mRNA. Translation: AAP40401.1.
IPI	IPI00536745.
PIR	F84771.
RefSeq	NP_565816.1. NM_129123.2.
UniGene	At.23626. At.71304.
3D structure databases
ProteinModelPortal	Q9ZQP1.
SMR	Q9ZQP1. Positions 73-215.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT2G35680.1-P.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G35680.1; AT2G35680.1; AT2G35680.
GeneID	818137.
KEGG	ath:AT2G35680.
Organism-specific databases
TAIR	At2g35680.
Phylogenomic databases
HOGENOM	HOG000238257.
InParanoid	Q9ZQP1.
OMA	NGNELWA.
PhylomeDB	Q9ZQP1.
ProtClustDB	CLSN2688713.
Gene expression databases
ArrayExpress	Q9ZQP1.
Genevestigator	Q9ZQP1.
Family and domain databases
InterPro	IPR000340. Dual-sp_phosphatase_cat-dom. IPR020422. Dual-sp_phosphatase_subgr_cat. IPR024950. DUSP. IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. [Graphical view]
PANTHER	PTHR10159. PTHR10159. 1 hit.
Pfam	PF00782. DSPc. 1 hit. [Graphical view]
PROSITE	PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50054. TYR_PHOSPHATASE_DUAL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DSP8_ARATH

Accession

Primary (citable) accession number: Q9ZQP1
Secondary accession number(s): Q8VZ64, Q940J5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2012
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program