Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9ZQG9

- E1314_ARATH

UniProt

Q9ZQG9 - E1314_ARATH

Protein

Glucan endo-1,3-beta-glucosidase 14

Gene

At2g27500

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 2 (01 Jun 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei267 – 2671NucleophileBy similarity
    Active sitei330 – 3301NucleophileBy similarity

    GO - Molecular functioni

    1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. defense response Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Cell wall biogenesis/degradation, Plant defense

    Enzyme and pathway databases

    BioCyciARA:AT2G27500-MONOMER.
    ARA:GQT-929-MONOMER.
    ARA:GQT-930-MONOMER.

    Protein family/group databases

    CAZyiGH17. Glycoside Hydrolase Family 17.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucan endo-1,3-beta-glucosidase 14 (EC:3.2.1.39)
    Alternative name(s):
    (1->3)-beta-glucan endohydrolase 14
    Short name:
    (1->3)-beta-glucanase 14
    Beta-1,3-endoglucanase 14
    Short name:
    Beta-1,3-glucanase 14
    Gene namesi
    Ordered Locus Names:At2g27500
    ORF Names:F10A12.18
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G27500.

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of plasma membrane Source: TAIR
    2. cell wall Source: UniProtKB-SubCell
    3. cytoplasm Source: TAIR
    4. extracellular region Source: UniProtKB-KW
    5. nucleus Source: TAIR

    Keywords - Cellular componenti

    Cell membrane, Cell wall, Cytoplasm, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 359338Glucan endo-1,3-beta-glucosidase 14PRO_0000251275Add
    BLAST
    Propeptidei360 – 39233Removed in mature formSequence AnalysisPRO_0000251276Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi54 – 541N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi89 – 891N-linked (GlcNAc...)Sequence Analysis
    Lipidationi359 – 3591GPI-anchor amidated serineSequence Analysis

    Keywords - PTMi

    Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PaxDbiQ9ZQG9.
    PRIDEiQ9ZQG9.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9ZQG9.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ZQG9.
    SMRiQ9ZQG9. Positions 29-346.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 17 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG240347.
    HOGENOMiHOG000238220.
    InParanoidiQ9ZQG9.
    OMAiIAMNQGT.
    PhylomeDBiQ9ZQG9.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00332. Glyco_hydro_17. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9ZQG9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MATHSLSFFF RVLLLLFLTL SERIKGQGVG INYGQIANNL PSPARVAVLL    50
    RSLNITRVKL YDADPNVLFS FSNSQVDFMI GLGNEYLQNM STDPTKAQDW 100
    LQQRLEPHIS KTRITSIVVG NEIFKTNDHV LIQSLLPAMK SVYAALTNLG 150
    LEKQVTVTSA HSLDILSTSY PPSSGSFKEE FIQYLQPLLD FHSQIESPFL 200
    INAYPFFAYK DSPKEVPLEY VLFQPNQGMV DPNTNLHYDN MLFAQVDALY 250
    SAIKTLGHTD IEVRISETGW PSKGDENEIG ASPENAALYN GNLLKLIQQR 300
    KGTPAKQSVP IDVYVFALFN ENLKPGPVSE RNYGLFYPDG KPVYNVGMQG 350
    YLPDIIYTSR ATTIKILNLW RVVMGLAVAW FILDMGDKMR MR 392
    Length:392
    Mass (Da):44,074
    Last modified:June 1, 2002 - v2
    Checksum:i67206B809BB7322A
    GO
    Isoform 2 (identifier: Q9ZQG9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         366-392: ILNLWRVVMGLAVAWFILDMGDKMRMR → VNSSFHFLYLHF

    Note: Derived from EST data. No experimental confirmation available. Has no GPI-anchor.

    Show »
    Length:377
    Mass (Da):42,348
    Checksum:i988CB3DD1BF4C700
    GO
    Isoform 3 (identifier: Q9ZQG9-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-78: Missing.

    Note: Derived from EST data. No experimental confirmation available. Has no GPI-anchor..

    Show »
    Length:314
    Mass (Da):35,376
    Checksum:i06B538965E38E34D
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 7878Missing in isoform 3. CuratedVSP_020754Add
    BLAST
    Alternative sequencei366 – 39227ILNLW…KMRMR → VNSSFHFLYLHF in isoform 2. CuratedVSP_020755Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006232 Genomic DNA. Translation: AAD15611.2.
    CP002685 Genomic DNA. Translation: AEC08004.1.
    CP002685 Genomic DNA. Translation: AEC08005.1.
    CP002685 Genomic DNA. Translation: AEC08006.1.
    AY096525 mRNA. Translation: AAM20175.1.
    AY065273 mRNA. Translation: AAL38749.1.
    AY065085 mRNA. Translation: AAL38261.1.
    AY084587 mRNA. Translation: AAM61152.1.
    PIRiF84673.
    RefSeqiNP_001031432.1. NM_001036355.1. [Q9ZQG9-3]
    NP_565652.1. NM_128310.3. [Q9ZQG9-1]
    NP_973548.1. NM_201819.2. [Q9ZQG9-2]
    UniGeneiAt.24289.

    Genome annotation databases

    EnsemblPlantsiAT2G27500.1; AT2G27500.1; AT2G27500. [Q9ZQG9-1]
    GeneIDi817295.
    KEGGiath:AT2G27500.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006232 Genomic DNA. Translation: AAD15611.2 .
    CP002685 Genomic DNA. Translation: AEC08004.1 .
    CP002685 Genomic DNA. Translation: AEC08005.1 .
    CP002685 Genomic DNA. Translation: AEC08006.1 .
    AY096525 mRNA. Translation: AAM20175.1 .
    AY065273 mRNA. Translation: AAL38749.1 .
    AY065085 mRNA. Translation: AAL38261.1 .
    AY084587 mRNA. Translation: AAM61152.1 .
    PIRi F84673.
    RefSeqi NP_001031432.1. NM_001036355.1. [Q9ZQG9-3 ]
    NP_565652.1. NM_128310.3. [Q9ZQG9-1 ]
    NP_973548.1. NM_201819.2. [Q9ZQG9-2 ]
    UniGenei At.24289.

    3D structure databases

    ProteinModelPortali Q9ZQG9.
    SMRi Q9ZQG9. Positions 29-346.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH17. Glycoside Hydrolase Family 17.

    Proteomic databases

    PaxDbi Q9ZQG9.
    PRIDEi Q9ZQG9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G27500.1 ; AT2G27500.1 ; AT2G27500 . [Q9ZQG9-1 ]
    GeneIDi 817295.
    KEGGi ath:AT2G27500.

    Organism-specific databases

    TAIRi AT2G27500.

    Phylogenomic databases

    eggNOGi NOG240347.
    HOGENOMi HOG000238220.
    InParanoidi Q9ZQG9.
    OMAi IAMNQGT.
    PhylomeDBi Q9ZQG9.

    Enzyme and pathway databases

    BioCyci ARA:AT2G27500-MONOMER.
    ARA:GQT-929-MONOMER.
    ARA:GQT-930-MONOMER.

    Gene expression databases

    Genevestigatori Q9ZQG9.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00332. Glyco_hydro_17. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    4. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    5. "Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins."
      Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C., Jensen O.N.
      Mol. Cell. Proteomics 2:1261-1270(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiE1314_ARATH
    AccessioniPrimary (citable) accession number: Q9ZQG9
    Secondary accession number(s): Q2V449, Q3EBS8, Q8VWJ3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2006
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 92 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3