Reviewed, UniProtKB/Swiss-Prot Q9ZQG9 (E1314_ARATH)

Names and origin

Protein names

Recommended name:
    Glucan endo-1,3-beta-glucosidase 14
    EC=3.2.1.39
Alternative name(s):
    (1->3)-beta-glucan endohydrolase 14
      Short name=(1->3)-beta-glucanase 14
    Beta-1,3-endoglucanase 14
      Short name=Beta-1,3-glucanase 14

Gene names

Name:	At2g27500/F10A12.18
Ordered Locus Names:	At2g27500

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Isoform 1: Cell membrane; Lipid-anchor › GPI-anchor; Extracellular side Probable. Secreted › cell wall Potential. Isoform 2: Secreted › cell wall Potential. Isoform 3: Cytoplasm Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Plant defense
Cellular component	Cell membrane Cell wall Cytoplasm Membrane Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	anchored to plasma membrane Ref.4 Inferred from direct assay. Source: TAIR cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from direct assay. Source: TAIR external side of plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from direct assay. Source: TAIR
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9ZQG9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9ZQG9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     366-392: ILNLWRVVMGLAVAWFILDMGDKMRMR → VNSSFHFLYLHF

Note: Derived from EST data. No experimental confirmation available. Has no GPI-anchor.

Isoform 3 (identifier: Q9ZQG9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-78: Missing.

Note: Derived from EST data. No experimental confirmation available. Has no GPI-anchor..

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 359	338	Glucan endo-1,3-beta-glucosidase 14		PRO_0000251275
	Propeptide	360 – 392	33	Removed in mature form Potential		PRO_0000251276
Sites
	Active site	267	1	Nucleophile By similarity
	Active site	330	1	Nucleophile By similarity
Amino acid modifications
	Lipidation	359	1	GPI-anchor amidated serine Potential
	Glycosylation	54	1	N-linked (GlcNAc...) Potential
	Glycosylation	89	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 78	78	Missing in isoform 3.		VSP_020754
	Alternative sequence	366 – 392	27	ILNLW…KMRMR → VNSSFHFLYLHF in isoform 2.		VSP_020755

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2002. Version 2. Checksum: 67206B809BB7322A	FASTA	392	44,074
	10 20 30 40 50 60 MATHSLSFFF RVLLLLFLTL SERIKGQGVG INYGQIANNL PSPARVAVLL RSLNITRVKL 70 80 90 100 110 120 YDADPNVLFS FSNSQVDFMI GLGNEYLQNM STDPTKAQDW LQQRLEPHIS KTRITSIVVG 130 140 150 160 170 180 NEIFKTNDHV LIQSLLPAMK SVYAALTNLG LEKQVTVTSA HSLDILSTSY PPSSGSFKEE 190 200 210 220 230 240 FIQYLQPLLD FHSQIESPFL INAYPFFAYK DSPKEVPLEY VLFQPNQGMV DPNTNLHYDN 250 260 270 280 290 300 MLFAQVDALY SAIKTLGHTD IEVRISETGW PSKGDENEIG ASPENAALYN GNLLKLIQQR 310 320 330 340 350 360 KGTPAKQSVP IDVYVFALFN ENLKPGPVSE RNYGLFYPDG KPVYNVGMQG YLPDIIYTSR 370 380 390 ATTIKILNLW RVVMGLAVAW FILDMGDKMR MR
	Isoform 2. Checksum: 988CB3DD1BF4C700	FASTA	377	42,348
	10 20 30 40 50 60 MATHSLSFFF RVLLLLFLTL SERIKGQGVG INYGQIANNL PSPARVAVLL RSLNITRVKL 70 80 90 100 110 120 YDADPNVLFS FSNSQVDFMI GLGNEYLQNM STDPTKAQDW LQQRLEPHIS KTRITSIVVG 130 140 150 160 170 180 NEIFKTNDHV LIQSLLPAMK SVYAALTNLG LEKQVTVTSA HSLDILSTSY PPSSGSFKEE 190 200 210 220 230 240 FIQYLQPLLD FHSQIESPFL INAYPFFAYK DSPKEVPLEY VLFQPNQGMV DPNTNLHYDN 250 260 270 280 290 300 MLFAQVDALY SAIKTLGHTD IEVRISETGW PSKGDENEIG ASPENAALYN GNLLKLIQQR 310 320 330 340 350 360 KGTPAKQSVP IDVYVFALFN ENLKPGPVSE RNYGLFYPDG KPVYNVGMQG YLPDIIYTSR 370 ATTIKVNSSF HFLYLHF
	Isoform 3. Checksum: 06B538965E38E34D	FASTA	314	35,376
	10 20 30 40 50 60 MIGLGNEYLQ NMSTDPTKAQ DWLQQRLEPH ISKTRITSIV VGNEIFKTND HVLIQSLLPA 70 80 90 100 110 120 MKSVYAALTN LGLEKQVTVT SAHSLDILST SYPPSSGSFK EEFIQYLQPL LDFHSQIESP 130 140 150 160 170 180 FLINAYPFFA YKDSPKEVPL EYVLFQPNQG MVDPNTNLHY DNMLFAQVDA LYSAIKTLGH 190 200 210 220 230 240 TDIEVRISET GWPSKGDENE IGASPENAAL YNGNLLKLIQ QRKGTPAKQS VPIDVYVFAL 250 260 270 280 290 300 FNENLKPGPV SERNYGLFYP DGKPVYNVGM QGYLPDIIYT SRATTIKILN LWRVVMGLAV 310 AWFILDMGDK MRMR

References

[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[3]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	"Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins." Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C., Jensen O.N. Mol. Cell. Proteomics 2:1261-1270(2003) [PubMed: 14517339] [Abstract] Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC006232 Genomic DNA. Translation: AAD15611.2. AY096525 mRNA. Translation: AAM20175.1. AY065273 mRNA. Translation: AAL38749.1. AY065085 mRNA. Translation: AAL38261.1. AY084587 mRNA. Translation: AAM61152.1.
IPI	IPI00529088. IPI00530832. IPI00656842.
PIR	F84673.
RefSeq	NP_001031432.1. NP_565652.1. NP_973548.1.
UniGene	At.24289
3D structure databases
HSSP	HSSP built from PDB template 1GHS based on UniProtKB P15737.
ModBase	Search...
Protein family/group databases
CAZy	GH17. Glycoside Hydrolase Family 17.
Proteomic databases
PRIDE	Q9ZQG9.
Genome annotation databases
GeneID	817295.
GenomeReviews	Gene locus AT2G27500 in contig CT485783_GR.
KEGG	ath:AT2G27500.
NMPDR	fig|3702.1.peg.9793.
Organism-specific databases
TAIR	At2g27500.
Phylogenomic databases
OMA	PATRITC.
Enzyme and pathway databases
BRENDA	3.2.1.39. 302.
Gene expression databases
ArrayExpress	Q9ZQG9.
Genevestigator	Q9ZQG9.
GermOnline	AT2G27500. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E1314_ARATH

Accession

Primary (citable) accession number: Q9ZQG9
Secondary accession number(s): Q2V449, Q3EBS8, Q8VWJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 50 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)