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Protein

Glucan endo-1,3-beta-glucosidase 14

Gene

At2g27500

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei267NucleophileBy similarity1
Active sitei330NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation, Plant defense

Enzyme and pathway databases

BioCyciARA:AT2G27500-MONOMER.

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase 14 (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase 14
Short name:
(1->3)-beta-glucanase 14
Beta-1,3-endoglucanase 14
Short name:
Beta-1,3-glucanase 14
Gene namesi
Ordered Locus Names:At2g27500
ORF Names:F10A12.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G27500.

Subcellular locationi

GO - Cellular componenti

  • anchored component of plasma membrane Source: TAIR
  • cell wall Source: UniProtKB-SubCell
  • cytoplasm Source: TAIR
  • extracellular region Source: UniProtKB-KW
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell wall, Cytoplasm, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000025127522 – 359Glucan endo-1,3-beta-glucosidase 14Add BLAST338
PropeptideiPRO_0000251276360 – 392Removed in mature formSequence analysisAdd BLAST33

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi54N-linked (GlcNAc...)Sequence analysis1
Glycosylationi89N-linked (GlcNAc...)Sequence analysis1
Lipidationi359GPI-anchor amidated serineSequence analysis1

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ9ZQG9.

Expressioni

Gene expression databases

GenevisibleiQ9ZQG9. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G27500.1.

Structurei

3D structure databases

ProteinModelPortaliQ9ZQG9.
SMRiQ9ZQG9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IH5G. Eukaryota.
ENOG410YA4N. LUCA.
HOGENOMiHOG000238220.
InParanoidiQ9ZQG9.
OMAiPHISKTR.
OrthoDBiEOG09360F5J.
PhylomeDBiQ9ZQG9.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9ZQG9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATHSLSFFF RVLLLLFLTL SERIKGQGVG INYGQIANNL PSPARVAVLL
60 70 80 90 100
RSLNITRVKL YDADPNVLFS FSNSQVDFMI GLGNEYLQNM STDPTKAQDW
110 120 130 140 150
LQQRLEPHIS KTRITSIVVG NEIFKTNDHV LIQSLLPAMK SVYAALTNLG
160 170 180 190 200
LEKQVTVTSA HSLDILSTSY PPSSGSFKEE FIQYLQPLLD FHSQIESPFL
210 220 230 240 250
INAYPFFAYK DSPKEVPLEY VLFQPNQGMV DPNTNLHYDN MLFAQVDALY
260 270 280 290 300
SAIKTLGHTD IEVRISETGW PSKGDENEIG ASPENAALYN GNLLKLIQQR
310 320 330 340 350
KGTPAKQSVP IDVYVFALFN ENLKPGPVSE RNYGLFYPDG KPVYNVGMQG
360 370 380 390
YLPDIIYTSR ATTIKILNLW RVVMGLAVAW FILDMGDKMR MR
Length:392
Mass (Da):44,074
Last modified:June 1, 2002 - v2
Checksum:i67206B809BB7322A
GO
Isoform 2 (identifier: Q9ZQG9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     366-392: ILNLWRVVMGLAVAWFILDMGDKMRMR → VNSSFHFLYLHF

Note: Derived from EST data. No experimental confirmation available. Has no GPI-anchor.
Show »
Length:377
Mass (Da):42,348
Checksum:i988CB3DD1BF4C700
GO
Isoform 3 (identifier: Q9ZQG9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-78: Missing.

Note: Derived from EST data. No experimental confirmation available. Has no GPI-anchor.
Show »
Length:314
Mass (Da):35,376
Checksum:i06B538965E38E34D
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0207541 – 78Missing in isoform 3. CuratedAdd BLAST78
Alternative sequenceiVSP_020755366 – 392ILNLW…KMRMR → VNSSFHFLYLHF in isoform 2. CuratedAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006232 Genomic DNA. Translation: AAD15611.2.
CP002685 Genomic DNA. Translation: AEC08004.1.
CP002685 Genomic DNA. Translation: AEC08005.1.
CP002685 Genomic DNA. Translation: AEC08006.1.
AY096525 mRNA. Translation: AAM20175.1.
AY065273 mRNA. Translation: AAL38749.1.
AY065085 mRNA. Translation: AAL38261.1.
AY084587 mRNA. Translation: AAM61152.1.
PIRiF84673.
RefSeqiNP_001031432.1. NM_001036355.1. [Q9ZQG9-3]
NP_565652.1. NM_128310.4. [Q9ZQG9-1]
NP_973548.1. NM_201819.2. [Q9ZQG9-2]
UniGeneiAt.24289.

Genome annotation databases

EnsemblPlantsiAT2G27500.1; AT2G27500.1; AT2G27500. [Q9ZQG9-1]
GeneIDi817295.
GrameneiAT2G27500.1; AT2G27500.1; AT2G27500.
KEGGiath:AT2G27500.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006232 Genomic DNA. Translation: AAD15611.2.
CP002685 Genomic DNA. Translation: AEC08004.1.
CP002685 Genomic DNA. Translation: AEC08005.1.
CP002685 Genomic DNA. Translation: AEC08006.1.
AY096525 mRNA. Translation: AAM20175.1.
AY065273 mRNA. Translation: AAL38749.1.
AY065085 mRNA. Translation: AAL38261.1.
AY084587 mRNA. Translation: AAM61152.1.
PIRiF84673.
RefSeqiNP_001031432.1. NM_001036355.1. [Q9ZQG9-3]
NP_565652.1. NM_128310.4. [Q9ZQG9-1]
NP_973548.1. NM_201819.2. [Q9ZQG9-2]
UniGeneiAt.24289.

3D structure databases

ProteinModelPortaliQ9ZQG9.
SMRiQ9ZQG9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G27500.1.

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Proteomic databases

PaxDbiQ9ZQG9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G27500.1; AT2G27500.1; AT2G27500. [Q9ZQG9-1]
GeneIDi817295.
GrameneiAT2G27500.1; AT2G27500.1; AT2G27500.
KEGGiath:AT2G27500.

Organism-specific databases

TAIRiAT2G27500.

Phylogenomic databases

eggNOGiENOG410IH5G. Eukaryota.
ENOG410YA4N. LUCA.
HOGENOMiHOG000238220.
InParanoidiQ9ZQG9.
OMAiPHISKTR.
OrthoDBiEOG09360F5J.
PhylomeDBiQ9ZQG9.

Enzyme and pathway databases

BioCyciARA:AT2G27500-MONOMER.

Miscellaneous databases

PROiQ9ZQG9.

Gene expression databases

GenevisibleiQ9ZQG9. AT.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiE1314_ARATH
AccessioniPrimary (citable) accession number: Q9ZQG9
Secondary accession number(s): Q2V449, Q3EBS8, Q8VWJ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: June 1, 2002
Last modified: November 30, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.