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Q9ZQG9 (E1314_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucan endo-1,3-beta-glucosidase 14

EC=3.2.1.39
Alternative name(s):
(1->3)-beta-glucan endohydrolase 14
Short name=(1->3)-beta-glucanase 14
Beta-1,3-endoglucanase 14
Short name=Beta-1,3-glucanase 14
Gene names
Ordered Locus Names:At2g27500
ORF Names:F10A12.18
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length392 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Subcellular location

Isoform 1: Cell membrane; Lipid-anchorGPI-anchor; Extracellular side Probable. Secretedcell wall Potential.

Isoform 2: Secretedcell wall Potential.

Isoform 3: Cytoplasm Potential.

Sequence similarities

Belongs to the glycosyl hydrolase 17 family.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9ZQG9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9ZQG9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     366-392: ILNLWRVVMGLAVAWFILDMGDKMRMR → VNSSFHFLYLHF
Note: Derived from EST data. No experimental confirmation available. Has no GPI-anchor.
Isoform 3 (identifier: Q9ZQG9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-78: Missing.
Note: Derived from EST data. No experimental confirmation available. Has no GPI-anchor..

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 359338Glucan endo-1,3-beta-glucosidase 14
PRO_0000251275
Propeptide360 – 39233Removed in mature form Potential
PRO_0000251276

Sites

Active site2671Nucleophile By similarity
Active site3301Nucleophile By similarity

Amino acid modifications

Lipidation3591GPI-anchor amidated serine Potential
Glycosylation541N-linked (GlcNAc...) Potential
Glycosylation891N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 7878Missing in isoform 3.
VSP_020754
Alternative sequence366 – 39227ILNLW…KMRMR → VNSSFHFLYLHF in isoform 2.
VSP_020755

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2002. Version 2.
Checksum: 67206B809BB7322A

FASTA39244,074
        10         20         30         40         50         60 
MATHSLSFFF RVLLLLFLTL SERIKGQGVG INYGQIANNL PSPARVAVLL RSLNITRVKL 

        70         80         90        100        110        120 
YDADPNVLFS FSNSQVDFMI GLGNEYLQNM STDPTKAQDW LQQRLEPHIS KTRITSIVVG 

       130        140        150        160        170        180 
NEIFKTNDHV LIQSLLPAMK SVYAALTNLG LEKQVTVTSA HSLDILSTSY PPSSGSFKEE 

       190        200        210        220        230        240 
FIQYLQPLLD FHSQIESPFL INAYPFFAYK DSPKEVPLEY VLFQPNQGMV DPNTNLHYDN 

       250        260        270        280        290        300 
MLFAQVDALY SAIKTLGHTD IEVRISETGW PSKGDENEIG ASPENAALYN GNLLKLIQQR 

       310        320        330        340        350        360 
KGTPAKQSVP IDVYVFALFN ENLKPGPVSE RNYGLFYPDG KPVYNVGMQG YLPDIIYTSR 

       370        380        390 
ATTIKILNLW RVVMGLAVAW FILDMGDKMR MR 

« Hide

Isoform 2 [UniParc].

Checksum: 988CB3DD1BF4C700
Show »

FASTA37742,348
Isoform 3 [UniParc].

Checksum: 06B538965E38E34D
Show »

FASTA31435,376

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[4]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]"Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins."
Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C., Jensen O.N.
Mol. Cell. Proteomics 2:1261-1270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC006232 Genomic DNA. Translation: AAD15611.2.
CP002685 Genomic DNA. Translation: AEC08004.1.
CP002685 Genomic DNA. Translation: AEC08005.1.
CP002685 Genomic DNA. Translation: AEC08006.1.
AY096525 mRNA. Translation: AAM20175.1.
AY065273 mRNA. Translation: AAL38749.1.
AY065085 mRNA. Translation: AAL38261.1.
AY084587 mRNA. Translation: AAM61152.1.
PIRF84673.
RefSeqNP_001031432.1. NM_001036355.1.
NP_565652.1. NM_128310.3.
NP_973548.1. NM_201819.2.
UniGeneAt.24289.

3D structure databases

ProteinModelPortalQ9ZQG9.
SMRQ9ZQG9. Positions 29-346.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH17. Glycoside Hydrolase Family 17.

Proteomic databases

PaxDbQ9ZQG9.
PRIDEQ9ZQG9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G27500.1; AT2G27500.1; AT2G27500. [Q9ZQG9-1]
GeneID817295.
KEGGath:AT2G27500.

Organism-specific databases

TAIRAT2G27500.

Phylogenomic databases

eggNOGNOG240347.
HOGENOMHOG000238220.
InParanoidQ9ZQG9.
OMANGNLMQR.
PhylomeDBQ9ZQG9.
ProtClustDBCLSN2688480.

Enzyme and pathway databases

BioCycARA:AT2G27500-MONOMER.
ARA:GQT-929-MONOMER.
ARA:GQT-930-MONOMER.

Gene expression databases

GenevestigatorQ9ZQG9.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameE1314_ARATH
AccessionPrimary (citable) accession number: Q9ZQG9
Secondary accession number(s): Q2V449, Q3EBS8, Q8VWJ3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: June 1, 2002
Last modified: April 16, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names