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Protein

Glucan endo-1,3-beta-glucosidase 14

Gene

At2g27500

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei267 – 2671NucleophileBy similarity
Active sitei330 – 3301NucleophileBy similarity

GO - Molecular functioni

  1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. cell wall organization Source: UniProtKB-KW
  3. defense response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation, Plant defense

Enzyme and pathway databases

BioCyciARA:AT2G27500-MONOMER.
ARA:GQT-929-MONOMER.
ARA:GQT-930-MONOMER.

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase 14 (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase 14
Short name:
(1->3)-beta-glucanase 14
Beta-1,3-endoglucanase 14
Short name:
Beta-1,3-glucanase 14
Gene namesi
Ordered Locus Names:At2g27500
ORF Names:F10A12.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G27500.

Subcellular locationi

GO - Cellular componenti

  1. anchored component of plasma membrane Source: TAIR
  2. cell wall Source: UniProtKB-SubCell
  3. cytoplasm Source: TAIR
  4. extracellular region Source: UniProtKB-KW
  5. nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell wall, Cytoplasm, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 359338Glucan endo-1,3-beta-glucosidase 14PRO_0000251275Add
BLAST
Propeptidei360 – 39233Removed in mature formSequence AnalysisPRO_0000251276Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi54 – 541N-linked (GlcNAc...)Sequence Analysis
Glycosylationi89 – 891N-linked (GlcNAc...)Sequence Analysis
Lipidationi359 – 3591GPI-anchor amidated serineSequence Analysis

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ9ZQG9.
PRIDEiQ9ZQG9.

Expressioni

Gene expression databases

GenevestigatoriQ9ZQG9.

Structurei

3D structure databases

ProteinModelPortaliQ9ZQG9.
SMRiQ9ZQG9. Positions 29-346.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG240347.
HOGENOMiHOG000238220.
InParanoidiQ9ZQG9.
OMAiPNQGTTD.
PhylomeDBiQ9ZQG9.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9ZQG9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATHSLSFFF RVLLLLFLTL SERIKGQGVG INYGQIANNL PSPARVAVLL
60 70 80 90 100
RSLNITRVKL YDADPNVLFS FSNSQVDFMI GLGNEYLQNM STDPTKAQDW
110 120 130 140 150
LQQRLEPHIS KTRITSIVVG NEIFKTNDHV LIQSLLPAMK SVYAALTNLG
160 170 180 190 200
LEKQVTVTSA HSLDILSTSY PPSSGSFKEE FIQYLQPLLD FHSQIESPFL
210 220 230 240 250
INAYPFFAYK DSPKEVPLEY VLFQPNQGMV DPNTNLHYDN MLFAQVDALY
260 270 280 290 300
SAIKTLGHTD IEVRISETGW PSKGDENEIG ASPENAALYN GNLLKLIQQR
310 320 330 340 350
KGTPAKQSVP IDVYVFALFN ENLKPGPVSE RNYGLFYPDG KPVYNVGMQG
360 370 380 390
YLPDIIYTSR ATTIKILNLW RVVMGLAVAW FILDMGDKMR MR
Length:392
Mass (Da):44,074
Last modified:June 1, 2002 - v2
Checksum:i67206B809BB7322A
GO
Isoform 2 (identifier: Q9ZQG9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     366-392: ILNLWRVVMGLAVAWFILDMGDKMRMR → VNSSFHFLYLHF

Note: Derived from EST data. No experimental confirmation available. Has no GPI-anchor.

Show »
Length:377
Mass (Da):42,348
Checksum:i988CB3DD1BF4C700
GO
Isoform 3 (identifier: Q9ZQG9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-78: Missing.

Note: Derived from EST data. No experimental confirmation available. Has no GPI-anchor..

Show »
Length:314
Mass (Da):35,376
Checksum:i06B538965E38E34D
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7878Missing in isoform 3. CuratedVSP_020754Add
BLAST
Alternative sequencei366 – 39227ILNLW…KMRMR → VNSSFHFLYLHF in isoform 2. CuratedVSP_020755Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006232 Genomic DNA. Translation: AAD15611.2.
CP002685 Genomic DNA. Translation: AEC08004.1.
CP002685 Genomic DNA. Translation: AEC08005.1.
CP002685 Genomic DNA. Translation: AEC08006.1.
AY096525 mRNA. Translation: AAM20175.1.
AY065273 mRNA. Translation: AAL38749.1.
AY065085 mRNA. Translation: AAL38261.1.
AY084587 mRNA. Translation: AAM61152.1.
PIRiF84673.
RefSeqiNP_001031432.1. NM_001036355.1. [Q9ZQG9-3]
NP_565652.1. NM_128310.3. [Q9ZQG9-1]
NP_973548.1. NM_201819.2. [Q9ZQG9-2]
UniGeneiAt.24289.

Genome annotation databases

EnsemblPlantsiAT2G27500.1; AT2G27500.1; AT2G27500. [Q9ZQG9-1]
GeneIDi817295.
KEGGiath:AT2G27500.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006232 Genomic DNA. Translation: AAD15611.2.
CP002685 Genomic DNA. Translation: AEC08004.1.
CP002685 Genomic DNA. Translation: AEC08005.1.
CP002685 Genomic DNA. Translation: AEC08006.1.
AY096525 mRNA. Translation: AAM20175.1.
AY065273 mRNA. Translation: AAL38749.1.
AY065085 mRNA. Translation: AAL38261.1.
AY084587 mRNA. Translation: AAM61152.1.
PIRiF84673.
RefSeqiNP_001031432.1. NM_001036355.1. [Q9ZQG9-3]
NP_565652.1. NM_128310.3. [Q9ZQG9-1]
NP_973548.1. NM_201819.2. [Q9ZQG9-2]
UniGeneiAt.24289.

3D structure databases

ProteinModelPortaliQ9ZQG9.
SMRiQ9ZQG9. Positions 29-346.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Proteomic databases

PaxDbiQ9ZQG9.
PRIDEiQ9ZQG9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G27500.1; AT2G27500.1; AT2G27500. [Q9ZQG9-1]
GeneIDi817295.
KEGGiath:AT2G27500.

Organism-specific databases

TAIRiAT2G27500.

Phylogenomic databases

eggNOGiNOG240347.
HOGENOMiHOG000238220.
InParanoidiQ9ZQG9.
OMAiPNQGTTD.
PhylomeDBiQ9ZQG9.

Enzyme and pathway databases

BioCyciARA:AT2G27500-MONOMER.
ARA:GQT-929-MONOMER.
ARA:GQT-930-MONOMER.

Miscellaneous databases

PROiQ9ZQG9.

Gene expression databases

GenevestigatoriQ9ZQG9.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. "Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins."
    Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C., Jensen O.N.
    Mol. Cell. Proteomics 2:1261-1270(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiE1314_ARATH
AccessioniPrimary (citable) accession number: Q9ZQG9
Secondary accession number(s): Q2V449, Q3EBS8, Q8VWJ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: June 1, 2002
Last modified: April 29, 2015
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.