Q9ZQG9 (E1314_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucan endo-1,3-beta-glucosidase 14
EC=3.2.1.39

Alternative name(s):
(1->3)-beta-glucan endohydrolase 14
Short name=(1->3)-beta-glucanase 14
Beta-1,3-endoglucanase 14
Short name=Beta-1,3-glucanase 14

Gene names

Ordered Locus Names:	At2g27500
ORF Names:	F10A12.18

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Isoform 1: Cell membrane; Lipid-anchor › GPI-anchor; Extracellular side Probable. Secreted › cell wall Potential. Isoform 2: Secreted › cell wall Potential. Isoform 3: Cytoplasm Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Plant defense
Cellular component	Cell membrane Cell wall Cytoplasm Membrane Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	anchored to plasma membrane Inferred from direct assay Ref.5. Source: TAIR cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from direct assay PubMed 15610358. Source: TAIR extracellular region Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from direct assay PubMed 15610358. Source: TAIR
Molecular_function	glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9ZQG9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9ZQG9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     366-392: ILNLWRVVMGLAVAWFILDMGDKMRMR → VNSSFHFLYLHF

Note: Derived from EST data. No experimental confirmation available. Has no GPI-anchor.

Isoform 3 (identifier: Q9ZQG9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-78: Missing.

Note: Derived from EST data. No experimental confirmation available. Has no GPI-anchor..

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 359	338	Glucan endo-1,3-beta-glucosidase 14		PRO_0000251275
	Propeptide	360 – 392	33	Removed in mature form Potential		PRO_0000251276
Sites
	Active site	267	1	Nucleophile By similarity
	Active site	330	1	Nucleophile By similarity
Amino acid modifications
	Lipidation	359	1	GPI-anchor amidated serine Potential
	Glycosylation	54	1	N-linked (GlcNAc...) Potential
	Glycosylation	89	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 78	78	Missing in isoform 3.		VSP_020754
	Alternative sequence	366 – 392	27	ILNLW…KMRMR → VNSSFHFLYLHF in isoform 2.		VSP_020755

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2002. Version 2. Checksum: 67206B809BB7322A	FASTA	392	44,074
	10 20 30 40 50 60 MATHSLSFFF RVLLLLFLTL SERIKGQGVG INYGQIANNL PSPARVAVLL RSLNITRVKL 70 80 90 100 110 120 YDADPNVLFS FSNSQVDFMI GLGNEYLQNM STDPTKAQDW LQQRLEPHIS KTRITSIVVG 130 140 150 160 170 180 NEIFKTNDHV LIQSLLPAMK SVYAALTNLG LEKQVTVTSA HSLDILSTSY PPSSGSFKEE 190 200 210 220 230 240 FIQYLQPLLD FHSQIESPFL INAYPFFAYK DSPKEVPLEY VLFQPNQGMV DPNTNLHYDN 250 260 270 280 290 300 MLFAQVDALY SAIKTLGHTD IEVRISETGW PSKGDENEIG ASPENAALYN GNLLKLIQQR 310 320 330 340 350 360 KGTPAKQSVP IDVYVFALFN ENLKPGPVSE RNYGLFYPDG KPVYNVGMQG YLPDIIYTSR 370 380 390 ATTIKILNLW RVVMGLAVAW FILDMGDKMR MR
	Isoform 2 [UniParc]. Checksum: 988CB3DD1BF4C700	FASTA	377	42,348
	10 20 30 40 50 60 MATHSLSFFF RVLLLLFLTL SERIKGQGVG INYGQIANNL PSPARVAVLL RSLNITRVKL 70 80 90 100 110 120 YDADPNVLFS FSNSQVDFMI GLGNEYLQNM STDPTKAQDW LQQRLEPHIS KTRITSIVVG 130 140 150 160 170 180 NEIFKTNDHV LIQSLLPAMK SVYAALTNLG LEKQVTVTSA HSLDILSTSY PPSSGSFKEE 190 200 210 220 230 240 FIQYLQPLLD FHSQIESPFL INAYPFFAYK DSPKEVPLEY VLFQPNQGMV DPNTNLHYDN 250 260 270 280 290 300 MLFAQVDALY SAIKTLGHTD IEVRISETGW PSKGDENEIG ASPENAALYN GNLLKLIQQR 310 320 330 340 350 360 KGTPAKQSVP IDVYVFALFN ENLKPGPVSE RNYGLFYPDG KPVYNVGMQG YLPDIIYTSR 370 ATTIKVNSSF HFLYLHF
	Isoform 3 [UniParc]. Checksum: 06B538965E38E34D	FASTA	314	35,376
	10 20 30 40 50 60 MIGLGNEYLQ NMSTDPTKAQ DWLQQRLEPH ISKTRITSIV VGNEIFKTND HVLIQSLLPA 70 80 90 100 110 120 MKSVYAALTN LGLEKQVTVT SAHSLDILST SYPPSSGSFK EEFIQYLQPL LDFHSQIESP 130 140 150 160 170 180 FLINAYPFFA YKDSPKEVPL EYVLFQPNQG MVDPNTNLHY DNMLFAQVDA LYSAIKTLGH 190 200 210 220 230 240 TDIEVRISET GWPSKGDENE IGASPENAAL YNGNLLKLIQ QRKGTPAKQS VPIDVYVFAL 250 260 270 280 290 300 FNENLKPGPV SERNYGLFYP DGKPVYNVGM QGYLPDIIYT SRATTIKILN LWRVVMGLAV 310 AWFILDMGDK MRMR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins." Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C., Jensen O.N. Mol. Cell. Proteomics 2:1261-1270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC006232 Genomic DNA. Translation: AAD15611.2. CP002685 Genomic DNA. Translation: AEC08004.1. CP002685 Genomic DNA. Translation: AEC08005.1. CP002685 Genomic DNA. Translation: AEC08006.1. AY096525 mRNA. Translation: AAM20175.1. AY065273 mRNA. Translation: AAL38749.1. AY065085 mRNA. Translation: AAL38261.1. AY084587 mRNA. Translation: AAM61152.1.
IPI	IPI00529088. IPI00530832. IPI00656842.
PIR	F84673.
RefSeq	NP_001031432.1. NM_001036355.1. NP_565652.1. NM_128310.3. NP_973548.1. NM_201819.2.
UniGene	At.24289.
3D structure databases
HSSP	HSSP built from PDB template 1GHS based on UniProtKB P15737.
ProteinModelPortal	Q9ZQG9.
SMR	Q9ZQG9. Positions 29-346.
ModBase	Search...
Protein family/group databases
CAZy	GH17. Glycoside Hydrolase Family 17.
Proteomic databases
PaxDb	Q9ZQG9.
PRIDE	Q9ZQG9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G27500.1; AT2G27500.1; AT2G27500.
GeneID	817295.
KEGG	ath:AT2G27500.
Organism-specific databases
TAIR	At2g27500.
Phylogenomic databases
eggNOG	NOG240347.
HOGENOM	HOG000238220.
InParanoid	Q9ZQG9.
OMA	NGNLMQR.
PhylomeDB	Q9ZQG9.
ProtClustDB	CLSN2688480.
Gene expression databases
Genevestigator	Q9ZQG9.
GermOnline	AT2G27500. Arabidopsis thaliana.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

E1314_ARATH

Accession

Primary (citable) accession number: Q9ZQG9
Secondary accession number(s): Q2V449, Q3EBS8, Q8VWJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program