Q9ZQG4 (U73B5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-glycosyltransferase 73B5
EC=2.4.1.-

Alternative name(s):
Flavonol 3-O-glucosyltransferase UGT73B5
EC=2.4.1.91

Gene names

Name:	UGT73B5
Ordered Locus Names:	At2g15480
ORF Names:	F9O13.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Possesses quercetin 3-O-glucosyltransferase activity in vitro. Involved in stress or defense responses. Ref.6 Ref.7
Catalytic activity	UDP-glucose + a flavonol = UDP + a flavonol 3-O-D-glucoside.
Tissue specificity	Expressed in roots and senescent leaves. Ref.7
Induction	Induced by pathogen infection, H2O2 and salicylic acid. Ref.7
Disruption phenotype	Decreased resistance to avirulent strains of P.syringae. Ref.7
Sequence similarities	Belongs to the UDP-glycosyltransferase family.
Sequence caution	The sequence AAM91525.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Plant defense
Coding sequence diversity	Alternative splicing
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response Inferred from electronic annotation. Source: UniProtKB-KW response to other organism Inferred from expression pattern Ref.7. Source: TAIR
Molecular_function	flavonol 3-O-glucosyltransferase activity Inferred from electronic annotation. Source: EC quercetin 3-O-glucosyltransferase activity Inferred from direct assay Ref.6. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9ZQG4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 484	484	UDP-glycosyltransferase 73B5		PRO_0000409080
Regions
	Region	356 – 358	3	UDP-glucose binding By similarity
	Region	373 – 381	9	UDP-glucose binding By similarity
	Region	395 – 398	4	UDP-glucose binding By similarity
Sites
	Binding site	297	1	UDP-glucose By similarity

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: B94342034C3394A6	FASTA	484	54,184
	10 20 30 40 50 60 MNREVSERIH ILFFPFMAQG HMIPILDMAK LFSRRGAKST LLTTPINAKI FEKPIEAFKN 70 80 90 100 110 120 QNPDLEIGIK IFNFPCVELG LPEGCENADF INSYQKSDSG DLFLKFLFST KYMKQQLESF 130 140 150 160 170 180 IETTKPSALV ADMFFPWATE SAEKLGVPRL VFHGTSFFSL CCSYNMRIHK PHKKVATSST 190 200 210 220 230 240 PFVIPGLPGD IVITEDQANV AKEETPMGKF MKEVRESETN SFGVLVNSFY ELESAYADFY 250 260 270 280 290 300 RSFVAKRAWH IGPLSLSNRE LGEKARRGKK ANIDEQECLK WLDSKTPGSV VYLSFGSGTN 310 320 330 340 350 360 FTNDQLLEIA FGLEGSGQSF IWVVRKNENQ GDNEEWLPEG FKERTTGKGL IIPGWAPQVL 370 380 390 400 410 420 ILDHKAIGGF VTHCGWNSAI EGIAAGLPMV TWPMGAEQFY NEKLLTKVLR IGVNVGATEL 430 440 450 460 470 480 VKKGKLISRA QVEKAVREVI GGEKAEERRL WAKKLGEMAK AAVEEGGSSY NDVNKFMEEL NGRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 35-484. Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 113-484. Strain: cv. Columbia.
[5]	"Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana." Li Y., Baldauf S., Lim E.K., Bowles D.J. J. Biol. Chem. 276:4338-4343(2001) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
[6]	"Arabidopsis glycosyltransferases as biocatalysts in fermentation for regioselective synthesis of diverse quercetin glucosides." Lim E.K., Ashford D.A., Hou B., Jackson R.G., Bowles D.J. Biotechnol. Bioeng. 87:623-631(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Pathogen-responsive expression of glycosyltransferase genes UGT73B3 and UGT73B5 is necessary for resistance to Pseudomonas syringae pv tomato in Arabidopsis." Langlois-Meurinne M., Gachon C.M., Saindrenan P. Plant Physiol. 139:1890-1901(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, INDUCTION BY PATHOGEN AND SALICYLIC ACID.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC006248 Genomic DNA. Translation: AAD17392.1. CP002685 Genomic DNA. Translation: AEC06405.1. AY128322 mRNA. Translation: AAM91525.1. Different initiation. BT015865 mRNA. Translation: AAU94428.1.
IPI	IPI00541429.
PIR	E84529.
RefSeq	NP_179150.3. NM_127108.3.
UniGene	At.40404.
3D structure databases
ProteinModelPortal	Q9ZQG4.
SMR	Q9ZQG4. Positions 9-478.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT2G15480.1-P.
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
Proteomic databases
PaxDb	Q9ZQG4.
PRIDE	Q9ZQG4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G15480.1; AT2G15480.1; AT2G15480.
GeneID	816040.
KEGG	ath:AT2G15480.
Organism-specific databases
TAIR	At2g15480.
Phylogenomic databases
eggNOG	NOG244246.
HOGENOM	HOG000237565.
InParanoid	Q9ZQG4.
PhylomeDB	Q9ZQG4.
ProtClustDB	PLN03007.
Gene expression databases
ArrayExpress	Q9ZQG4.
Genevestigator	Q9ZQG4.
Family and domain databases
InterPro	IPR002213. UDP_glucos_trans. [Graphical view]
PANTHER	PTHR11926. PTHR11926. 1 hit.
Pfam	PF00201. UDPGT. 1 hit. [Graphical view]
PROSITE	PS00375. UDPGT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

U73B5_ARATH

Accession

Primary (citable) accession number: Q9ZQG4
Secondary accession number(s): Q8L7Q5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 31, 2011
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program