Probable beta-1,4-xylosyltransferase IRX9 - Arabidopsis thaliana (Mouse-ear cress)

Contribute

Send feedback

Read comments (?) or add your own

Q9ZQC6 (IRX9_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 59. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Probable beta-1,4-xylosyltransferase IRX9
EC=2.4.2.-

Alternative name(s):
Protein IRREGULAR XYLEM 9
Xylan xylosyltransferase IRX9

Gene names

Name:	IRX9
Ordered Locus Names:	At2g37090
ORF Names:	T2N18.15

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the synthesis of the hemicellulose glucuronoxylan, a major component of secondary cell walls. Probably involved in the elongation of glucuronoxylan xylosyl backbone. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Probable Ref.8.
Tissue specificity	Expressed in developing interfascicular fibers, primary and secondary xylem in stems and developing secondary xylem in roots. Ref.8
Disruption phenotype	Dwarf phenotype. Collapsed xylem vessels and reduced xylan content in cell wall. Ref.5 Ref.6 Ref.7 Ref.8
Sequence similarities	Belongs to the glycosyltransferase 43 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW glucuronoxylan biosynthetic process Inferred from genetic interaction. Source: TAIR secondary cell wall biogenesis Inferred from mutant phenotype Ref.5. Source: TAIR
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity Inferred from electronic annotation. Source: InterPro xylosyltransferase activity Inferred from mutant phenotype Ref.7. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 351

351

Probable beta-1,4-xylosyltransferase IRX9

PRO_0000407563

Regions

Topological domain

1 – 16

Cytoplasmic Potential

Transmembrane

17 – 36

Helical; Signal-anchor for type II membrane protein; Potential

Topological domain

37 – 351

315

Lumenal Potential

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

271

N-linked (GlcNAc...) Potential

Glycosylation

287

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q9ZQC6 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 1F0F48D43B8A3DA1
Show »

FASTA

351

40,059

        10         20         30         40         50         60 
MGSLERSKKK AQVWKKAVIH FSLCFVMGFF TGFAPAGKAS FFSNFETTSY TSTKSPIPPQ 

        70         80         90        100        110        120 
PFENATYTQH SLLNRTLINS QSQAPAPAES REAEGETRSL SEKEDENQVK VTPRGLVIVV 

       130        140        150        160        170        180 
TPIITKDRYK NVLLRRMANT LRLVPPPLLW IVVEKHSDGE EKSSSTMLRK TGIMYRRIVF 

       190        200        210        220        230        240 
KEDFTSLESE LDHQRNLALR HIEHHKLSGI VHFAGLNNIY DLDFFVKIRD IEVFGTWPMA 

       250        260        270        280        290        300 
LLSANRKRVV VEGPVCESSQ VLGWHLRKIN NETETKPPIH ISSFAFNSSI LWDPERWGRP 

       310        320        330        340        350 
SSVEGTKQDS IKYVKQVVLE DDTKLKGLPA QDCSKIMLWR LKFPTRTRLS T

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Arabidopsis ORF clones." Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Kim C.J., Chen H., Cheuk R., Shinn P., Ecker J.R. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Identification of novel genes in Arabidopsis involved in secondary cell wall formation using expression profiling and reverse genetics." Brown D.M., Zeef L.A.H., Ellis J., Goodacre R., Turner S.R. Plant Cell 17:2281-2295(2005) [PubMed: 15980264] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[6]	"Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls." Bauer S., Vasu P., Persson S., Mort A.J., Somerville C.R. Proc. Natl. Acad. Sci. U.S.A. 103:11417-11422(2006) [PubMed: 16844780] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[7]	"Comparison of five xylan synthesis mutants reveals new insight into the mechanisms of xylan synthesis." Brown D.M., Goubet F., Wong V.W., Goodacre R., Stephens E., Dupree P., Turner S.R. Plant J. 52:1154-1168(2007) [PubMed: 17944810] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[8]	"Arabidopsis irregular xylem8 and irregular xylem9: implications for the complexity of glucuronoxylan biosynthesis." Pena M.J., Zhong R., Zhou G.K., Richardson E.A., O'Neill M.A., Darvill A.G., York W.S., Ye Z.H. Plant Cell 19:549-563(2007) [PubMed: 17322407] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[9]	"The irregular xylem9 mutant is deficient in xylan xylosyltransferase activity." Lee C., O'Neill M.A., Tsumuraya Y., Darvill A.G., Ye Z.H. Plant Cell Physiol. 48:1624-1634(2007) [PubMed: 17938130] [Abstract] Cited for: FUNCTION.
[10]	"The Arabidopsis family GT43 glycosyltransferases form two functionally nonredundant groups essential for the elongation of glucuronoxylan backbone." Lee C., Teng Q., Huang W., Zhong R., Ye Z.H. Plant Physiol. 153:526-541(2010) [PubMed: 20335400] [Abstract] Cited for: FUNCTION.
[11]	"Analysis of the Arabidopsis IRX9/IRX9-L and IRX14/IRX14-L pairs of glycosyltransferase genes reveals critical contributions to biosynthesis of the hemicellulose glucuronoxylan." Wu A.M., Hoernblad E., Voxeur A., Gerber L., Rihouey C., Lerouge P., Marchant A. Plant Physiol. 153:542-554(2010) [PubMed: 20424005] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC006260 Genomic DNA. Translation: AAD18150.1. CP002685 Genomic DNA. Translation: AEC09350.1. BT015044 mRNA. Translation: AAT71916.1. BT015838 mRNA. Translation: AAU94401.1.
IPI	IPI00532147.
PIR	D84788.
RefSeq	NP_181246.1. NM_129265.2.
UniGene	At.26964. At.67134.
3D structure databases
ProteinModelPortal	Q9ZQC6.
SMR	Q9ZQC6. Positions 115-350.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9ZQC6.
Protein family/group databases
CAZy	GT43. Glycosyltransferase Family 43.
Proteomic databases
PRIDE	Q9ZQC6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G37090.1; AT2G37090.1; AT2G37090.
GeneID	818285.
GenomeReviews	Gene locus AT2G37090 in contig CT485783_GR.
KEGG	ath:AT2G37090.
NMPDR	fig\|3702.1.peg.10812.
Organism-specific databases
TAIR	At2g37090.
Phylogenomic databases
eggNOG	KOG1476.
GeneTree	EPGT00050000027792.
HOGENOM	HBG600303.
InParanoid	Q9ZQC6.
OMA	FSLCFVM.
PhylomeDB	Q9ZQC6.
ProtClustDB	PLN02458.
Gene expression databases
ArrayExpress	Q9ZQC6.
Genevestigator	Q9ZQC6.
Family and domain databases
InterPro	IPR005027. Glyco_trans_43. [Graphical view]
PANTHER	PTHR10896. Glyco_trans_43. 1 hit.
Pfam	PF03360. Glyco_transf_43. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IRX9_ARATH

Accession

Primary (citable) accession number: Q9ZQC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 3, 2011
Last sequence update:	May 1, 1999
Last modified:	September 21, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents