Reviewed, UniProtKB/Swiss-Prot Q9ZQA4 (PME14_ARATH)

Names and origin

Protein names

Recommended name:
    Putative pectinesterase 14
      Short name=PE 14
    EC=3.1.1.11
Alternative name(s):
    Pectin methylesterase 14
      Short name=AtPME14

Gene names

Name:	PME14
Synonyms:	ARATH14
Ordered Locus Names:	At2g36700
ORF Names:	F13K3.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Acts in the modification of cell walls via demethylesterification of cell wall pectin By similarity.
Catalytic activity	Pectin + n H2O = n methanol + pectate.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
Subcellular location	Secreted › cell wall By similarity.
Tissue specificity	Expressed in flower buds. Ref.3
Sequence similarities	Belongs to the pectinesterase family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Aspartyl esterase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall modification Inferred from electronic annotation. Source: InterPro
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aspartyl esterase activity Inferred from electronic annotation. Source: UniProtKB-KW pectinesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 333	317	Putative pectinesterase 14		PRO_0000371671
Sites
	Active site	174	1	Proton donor By similarity
	Active site	195	1	Nucleophile By similarity
	Binding site	116	1	Substrate By similarity
	Binding site	151	1	Substrate By similarity
	Binding site	253	1	Substrate By similarity
	Site	173	1	Transition state stabilizer By similarity
Amino acid modifications
	Glycosylation	108	1	N-linked (GlcNAc...) Potential
	Glycosylation	114	1	N-linked (GlcNAc...) Potential
	Glycosylation	133	1	N-linked (GlcNAc...) Potential
	Glycosylation	302	1	N-linked (GlcNAc...) Potential
	Glycosylation	323	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9ZQA4-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 5751439F1DF535B7	FASTA	333	37,067
	10 20 30 40 50 60 MLFFILFLSI ISPIESVDQR IHHPSKCDHL SKFPTKGFTM VLKVSLNGCG RFKRVQDAID 70 80 90 100 110 120 ASIGSSQSKT LILIDFGIYR ERFIVHENKN NLVVQGMGYS RTSIEWNNTT ASSNGTFSSF 130 140 150 160 170 180 SVAVFGEKFT AYNISFKNTA PAPNPGAVDA QAVALKVVGD KAAFYGCGFY GNQDTLLDQE 190 200 210 220 230 240 GRHFFKGCFI EGSIDFIFGN GRSLYEDCTL HSIAKENTIG CITANGKDTL KDRTGFVFVN 250 260 270 280 290 300 CKITGSARVW LGRAWRPYAR VIFSKTYMSR VVSLDGWNDM GDPKTQRTVY YGEHRCYGPG 310 320 330 ANHSKRVTYA KLLSDVEAAP FTNISFIDGE EWL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Pectin methylesterases: sequence-structural features and phylogenetic relationships." Markovic O., Janecek S. Carbohydr. Res. 339:2281-2295(2004) [PubMed: 15337457] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[3]	"Comprehensive expression profiling of the pectin methylesterase gene family during silique development in Arabidopsis thaliana." Louvet R., Cavel E., Gutierrez L., Guenin S., Roger D., Gillet F., Guerineau F., Pelloux J. Planta 224:782-791(2006) [PubMed: 16622707] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC006282 Genomic DNA. Translation: AAD20146.1.
IPI	IPI00548967.
PIR	F84783.
RefSeq	NP_181208.1.
UniGene	At.53054
3D structure databases
HSSP	HSSP built from PDB template 1GQ8 based on UniProtKB P83218.
SMR	Q9ZQA4. Positions 41-333.
ModBase	Search...
Genome annotation databases
GeneID	818242.
GenomeReviews	Gene locus AT2G36700 in contig CT485783_GR.
KEGG	ath:AT2G36700.
NMPDR	fig|3702.1.peg.10774.
Organism-specific databases
GeneFarm	451. 8.
TAIR	At2g36700.
Phylogenomic databases
eggNOG	euNOG09608.
HOGENOM	HBG747179.
InParanoid	Q9ZQA4.
OMA	SARIYET.
Gene expression databases
ArrayExpress	Q9ZQA4.
Genevestigator	Q9ZQA4.
Family and domain databases
InterPro	IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. IPR018040. Pectinesterase_AS. IPR000070. Pectinesterase_cat. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF01095. Pectinesterase. 1 hit. [Graphical view]
PROSITE	PS00800. PECTINESTERASE_1. False negative. PS00503. PECTINESTERASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PME14_ARATH

Accession

Primary (citable) accession number: Q9ZQA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	May 1, 1999
Last modified:	February 9, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)