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Protein

UDP-glycosyltransferase 73C5

Gene

UGT73C5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Specifically catalyzes 23-O-glucosylation of brassinosteroids, resulting probably in their inactivation. Also, involved in the O-glucosylation of trans-zeatin and dihydrozeatin. Active in vitro on cis-zeatin, dihydrozeatin-9-N-Glc, and olomoucine. Also involved in the detoxification of the Fusarium mycotoxin deoxynivalenol by the transfer of glucose from UDP-glucose to the hydroxyl group at C-3. Possesses low quercetin 7-O-glucosyltransferase and 4'-O-glucosyltransferase activities in vitro.4 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei296 – 2961UDP-glucoseBy similarity

GO - Molecular functioni

  • cis-zeatin O-beta-D-glucosyltransferase activity Source: TAIR
  • glucosyltransferase activity Source: TAIR
  • quercetin 3-O-glucosyltransferase activity Source: GO_Central
  • quercetin 4'-O-glucosyltransferase activity Source: TAIR
  • quercetin 7-O-glucosyltransferase activity Source: TAIR
  • trans-zeatin O-beta-D-glucosyltransferase activity Source: TAIR

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:AT2G36800-MONOMER.
BRENDAi2.4.1.203. 399.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glycosyltransferase 73C5 (EC:2.4.1.-)
Alternative name(s):
Cytokinin-O-glucosyltransferase 3
Deoxynivalenol-glucosyl-transferase 1
Zeatin O-glucosyltransferase 3
Short name:
AtZOG3
Gene namesi
Name:UGT73C5
Synonyms:DOGT1, ZOG3
Ordered Locus Names:At2g36800
ORF Names:F13K3.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G36800.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei146 – 16217HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 495495UDP-glycosyltransferase 73C5PRO_0000074157Add
BLAST

Proteomic databases

PaxDbiQ9ZQ94.
PRIDEiQ9ZQ94.

PTM databases

iPTMnetiQ9ZQ94.

Expressioni

Tissue specificityi

Elongating hypocotyls and root-specific. Expressed in the vascular system, in meristematic tissues of the root tip, and in the vasculature of the hypocotyl right after germination. In late stage of flower development, expressed in petals, and in abscission zones.2 Publications

Inductioni

Rapidly induced in response to deoxynivalenol exposure. Weak induction by salicylic acid, jasmonic acid and 1-aminocyclopropylcarbonic acid (ACC) treatments. Not induced by cytokinin treatment.2 Publications

Gene expression databases

ExpressionAtlasiQ9ZQ94. baseline and differential.
GenevisibleiQ9ZQ94. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G36800.1.

Structurei

3D structure databases

ProteinModelPortaliQ9ZQ94.
SMRiQ9ZQ94. Positions 12-483.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni356 – 3583UDP-glucose bindingBy similarity
Regioni373 – 3819UDP-glucose bindingBy similarity
Regioni395 – 3984UDP-glucose bindingBy similarity

Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1192. Eukaryota.
COG1819. LUCA.
HOGENOMiHOG000237565.
InParanoidiQ9ZQ94.
KOiK13496.
OMAiPDKIQVT.
PhylomeDBiQ9ZQ94.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZQ94-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSETTKSSP LHFVLFPFMA QGHMIPMVDI ARLLAQRGVI ITIVTTPHNA
60 70 80 90 100
ARFKNVLNRA IESGLPINLV QVKFPYLEAG LQEGQENIDS LDTMERMIPF
110 120 130 140 150
FKAVNFLEEP VQKLIEEMNP RPSCLISDFC LPYTSKIAKK FNIPKILFHG
160 170 180 190 200
MGCFCLLCMH VLRKNREILD NLKSDKELFT VPDFPDRVEF TRTQVPVETY
210 220 230 240 250
VPAGDWKDIF DGMVEANETS YGVIVNSFQE LEPAYAKDYK EVRSGKAWTI
260 270 280 290 300
GPVSLCNKVG ADKAERGNKS DIDQDECLKW LDSKKHGSVL YVCLGSICNL
310 320 330 340 350
PLSQLKELGL GLEESQRPFI WVIRGWEKYK ELVEWFSESG FEDRIQDRGL
360 370 380 390 400
LIKGWSPQML ILSHPSVGGF LTHCGWNSTL EGITAGLPLL TWPLFADQFC
410 420 430 440 450
NEKLVVEVLK AGVRSGVEQP MKWGEEEKIG VLVDKEGVKK AVEELMGESD
460 470 480 490
DAKERRRRAK ELGDSAHKAV EEGGSSHSNI SFLLQDIMEL AEPNN
Length:495
Mass (Da):55,798
Last modified:May 1, 1999 - v1
Checksum:iAAC9727F48C069A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY573822 Genomic DNA. Translation: AAS87592.1.
AC006282 Genomic DNA. Translation: AAD20156.1.
CP002685 Genomic DNA. Translation: AEC09299.1.
AY062743 mRNA. Translation: AAL32821.1.
BT003373 mRNA. Translation: AAO30036.1.
PIRiH84784.
RefSeqiNP_181218.1. NM_129235.3.
UniGeneiAt.27247.

Genome annotation databases

EnsemblPlantsiAT2G36800.1; AT2G36800.1; AT2G36800.
GeneIDi818252.
GrameneiAT2G36800.1; AT2G36800.1; AT2G36800.
KEGGiath:AT2G36800.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY573822 Genomic DNA. Translation: AAS87592.1.
AC006282 Genomic DNA. Translation: AAD20156.1.
CP002685 Genomic DNA. Translation: AEC09299.1.
AY062743 mRNA. Translation: AAL32821.1.
BT003373 mRNA. Translation: AAO30036.1.
PIRiH84784.
RefSeqiNP_181218.1. NM_129235.3.
UniGeneiAt.27247.

3D structure databases

ProteinModelPortaliQ9ZQ94.
SMRiQ9ZQ94. Positions 12-483.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G36800.1.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

PTM databases

iPTMnetiQ9ZQ94.

Proteomic databases

PaxDbiQ9ZQ94.
PRIDEiQ9ZQ94.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G36800.1; AT2G36800.1; AT2G36800.
GeneIDi818252.
GrameneiAT2G36800.1; AT2G36800.1; AT2G36800.
KEGGiath:AT2G36800.

Organism-specific databases

TAIRiAT2G36800.

Phylogenomic databases

eggNOGiKOG1192. Eukaryota.
COG1819. LUCA.
HOGENOMiHOG000237565.
InParanoidiQ9ZQ94.
KOiK13496.
OMAiPDKIQVT.
PhylomeDBiQ9ZQ94.

Enzyme and pathway databases

BioCyciMetaCyc:AT2G36800-MONOMER.
BRENDAi2.4.1.203. 399.

Miscellaneous databases

PROiQ9ZQ94.

Gene expression databases

ExpressionAtlasiQ9ZQ94. baseline and differential.
GenevisibleiQ9ZQ94. AT.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis genes encoding zeatin O-glucosyltransferases."
    Martin R.C., Mok M.C., Mok D.W.S.
    Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana."
    Li Y., Baldauf S., Lim E.K., Bowles D.J.
    J. Biol. Chem. 276:4338-4343(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  6. "Detoxification of the Fusarium mycotoxin deoxynivalenol by a UDP-glucosyltransferase from Arabidopsis thaliana."
    Poppenberger B., Berthiller F., Lucyshyn D., Sieberer T., Schuhmacher R., Krska R., Kuchler K., Gloessl J., Luschnig C., Adam G.
    J. Biol. Chem. 278:47905-47914(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY.
  7. "Arabidopsis glycosyltransferases as biocatalysts in fermentation for regioselective synthesis of diverse quercetin glucosides."
    Lim E.K., Ashford D.A., Hou B., Jackson R.G., Bowles D.J.
    Biotechnol. Bioeng. 87:623-631(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "A class of plant glycosyltransferases involved in cellular homeostasis."
    Lim E.K., Bowles D.J.
    EMBO J. 23:2915-2922(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLASSIFICATION, NOMENCLATURE.
  9. "N-glucosylation of cytokinins by glycosyltransferases of Arabidopsis thaliana."
    Hou B., Lim E.-K., Higgins G.S., Bowles D.J.
    J. Biol. Chem. 279:47822-47832(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  10. Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiU73C5_ARATH
AccessioniPrimary (citable) accession number: Q9ZQ94
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2005
Last sequence update: May 1, 1999
Last modified: February 17, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.