Reviewed, UniProtKB/Swiss-Prot Q9ZPY2 (LAC6_ARATH)

Names and origin

Protein names

Recommended name:
    Laccase-6
    EC=1.10.3.2
Alternative name(s):
    Benzenediol:oxygen oxidoreductase 6
    Urishiol oxidase 6
    Diphenol oxidase 6

Gene names

Name:	LAC6
Ordered Locus Names:	At2g46570
ORF Names:	F13A10.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	569 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Lignin degradation and detoxification of lignin-derived products By similarity.
Catalytic activity	4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.
Cofactor	Binds 4 copper ions per monomer By similarity.
Subcellular location	Secreted › extracellular space › apoplast Potential.
Tissue specificity	Predominantly expressed in the inflorescence stem, but not in siliques. Ref.3 Ref.4
Sequence similarities	Belongs to the multicopper oxidase family. Contains 3 plastocyanin-like domains.

Ontologies

Keywords
Biological process	Lignin degradation
Cellular component	Apoplast Secreted
Coding sequence diversity	Alternative splicing
Domain	Repeat Signal
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW laccase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9ZPY2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 (identifier: Q9ZPY2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-235: Missing.
     296-327: Missing.
     528-568: LWLLHCHFDIHQTWGMSTMFIVKNGKKVQESLPHPPADLPK → ILLHKTPKHHNTYDI

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 569	540	Laccase-6		PRO_0000283634
Regions
	Domain	37 – 153	117	Plastocyanin-like 1
	Domain	163 – 315	153	Plastocyanin-like 2
	Domain	417 – 553	137	Plastocyanin-like 3
Sites
	Metal binding	87	1	Copper 1; type 2 By similarity
	Metal binding	89	1	Copper 2; type 3 By similarity
	Metal binding	132	1	Copper 2; type 3 By similarity
	Metal binding	134	1	Copper 3; type 3 By similarity
	Metal binding	472	1	Copper 4; type 1 By similarity
	Metal binding	475	1	Copper 1; type 2 By similarity
	Metal binding	477	1	Copper 3; type 3 By similarity
	Metal binding	532	1	Copper 3; type 3 By similarity
	Metal binding	533	1	Copper 4; type 1 By similarity
	Metal binding	534	1	Copper 2; type 3 By similarity
	Metal binding	538	1	Copper 4; type 1 By similarity
	Metal binding	543	1	Copper 4; type 1 By similarity
Amino acid modifications
	Glycosylation	83	1	N-linked (GlcNAc...) Potential
	Glycosylation	208	1	N-linked (GlcNAc...) Potential
	Glycosylation	303	1	N-linked (GlcNAc...) Potential
	Glycosylation	319	1	N-linked (GlcNAc...) Potential
	Glycosylation	392	1	N-linked (GlcNAc...) Potential
	Glycosylation	438	1	N-linked (GlcNAc...) Potential
	Glycosylation	444	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 235	235	Missing in isoform 2.		VSP_024345
	Alternative sequence	296 – 327	32	Missing in isoform 2.		VSP_024346
	Alternative sequence	528 – 568	41	LWLLH…ADLPK → ILLHKTPKHHNTYDI in isoform 2.		VSP_024347

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 3BA02CB6A0C44238	FASTA	569	63,901
	10 20 30 40 50 60 MTSSAVPSLF RLSFLLFTLQ VMNIGRIGAA TRFYQFKVQT IRLTRLCQTN EIVTVNKKFP 70 80 90 100 110 120 GPAISAQEDD RIVIKVINMT PYNTTIHWHG IKQKRSCWYD GPSYITQCPI QSGQSFTYNF 130 140 150 160 170 180 KVAQQKGTFL WHAHFSWLRA TVYGPLIVYP KASVPYPFKK PFNEHTILLG EYWLKNVVEL 190 200 210 220 230 240 EQHVLESGGP PPPADAFTIN GQPGPNYNCS SKDVYEIQIV PRKIYLLRLI NAGINMETFF 250 260 270 280 290 300 TIANHRLTIV EVDGEYTKPY TTERVMLVPG QTMNILVTAD QTVGRYSMAM GPYESAKNVK 310 320 330 340 350 360 FQNTSAIANF QYIGALPNNV TVPAKLPIFN DNIAVKTVMD GLRSLNAVDV PRNIDAHLFI 370 380 390 400 410 420 TIGLNVNKCN SENPNNKCQG PRKGRLAASM NNISFIEPKV SILEAYYKQL EGYFTLDFPT 430 440 450 460 470 480 TPEKAYDFVN GAPNDIANDT QAANGTRAIV FEYGSRIQII FQNTGTLTTE NHPIHLHGHS 490 500 510 520 530 540 FYVIGYGTGN YDQQTAKFNL EDPPYLNTIG VPVGGWAAIR FVANNPGLWL LHCHFDIHQT 550 560 WGMSTMFIVK NGKKVQESLP HPPADLPKC
	Isoform 2. Checksum: 08DB7D61D8BB37B1	FASTA	276	30,742
	10 20 30 40 50 60 METFFTIANH RLTIVEVDGE YTKPYTTERV MLVPGQTMNI LVTADQTVGR YSMAMGPYES 70 80 90 100 110 120 IFNDNIAVKT VMDGLRSLNA VDVPRNIDAH LFITIGLNVN KCNSENPNNK CQGPRKGRLA 130 140 150 160 170 180 ASMNNISFIE PKVSILEAYY KQLEGYFTLD FPTTPEKAYD FVNGAPNDIA NDTQAANGTR 190 200 210 220 230 240 AIVFEYGSRI QIIFQNTGTL TTENHPIHLH GHSFYVIGYG TGNYDQQTAK FNLEDPPYLN 250 260 270 TIGVPVGGWA AIRFVANNPG ILLHKTPKHH NTYDIC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[3]	"Gene structure and molecular analysis of the laccase-like multicopper oxidase (LMCO) gene family in Arabidopsis thaliana." McCaig B.C., Meagher R.B., Dean J.F.D. Planta 221:619-636(2005) [PubMed: 15940465] [Abstract] Cited for: TISSUE SPECIFICITY.
[4]	"Mutant identification and characterization of the laccase gene family in Arabidopsis." Cai X., Davis E.J., Ballif J., Liang M., Bushman E., Haroldsen V., Torabinejad J., Wu Y. J. Exp. Bot. 57:2563-2569(2006) [PubMed: 16804053] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC006418 Genomic DNA. Translation: AAD20177.1. AY078937 mRNA. Translation: AAL84943.1.
IPI	IPI00536099. IPI00845129.
PIR	E84904.
RefSeq	NP_182180.1.
UniGene	At.53141
3D structure databases
HSSP	HSSP built from PDB template 1AOZ based on UniProtKB P37064.
ModBase	Search...
Genome annotation databases
GeneID	819269.
GenomeReviews	Gene locus AT2G46570 in contig CT485783_GR.
KEGG	ath:AT2G46570.
NMPDR	fig|3702.1.peg.11842.
Organism-specific databases
TAIR	At2g46570.
Phylogenomic databases
eggNOG	KOG1263.
HOGENOM	HBG749556.
InParanoid	Q9ZPY2.
OMA	MNNISFI.
PhylomeDB	Q9ZPY2.
Enzyme and pathway databases
BRENDA	1.10.3.2. 302.
Gene expression databases
ArrayExpress	Q9ZPY2.
Genevestigator	Q9ZPY2.
Family and domain databases
InterPro	IPR001117. Cu-oxidase. IPR011706. Cu-oxidase_2. IPR011707. Cu-oxidase_3. IPR002355. Cu_oxidase_Cu_BS. IPR008972. Cupredoxin. IPR017761. Laccase. [Graphical view]
Gene3D	G3DSA:2.60.40.420. Cupredoxin. 3 hits.
Pfam	PF00394. Cu-oxidase. 1 hit. PF07731. Cu-oxidase_2. 1 hit. PF07732. Cu-oxidase_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR03389. laccase. 1 hit.
PROSITE	PS00079. MULTICOPPER_OXIDASE1. 1 hit. PS00080. MULTICOPPER_OXIDASE2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LAC6_ARATH

Accession

Primary (citable) accession number: Q9ZPY2
Secondary accession number(s): Q8RY28

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	May 1, 1999
Last modified:	February 9, 2010
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)