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Protein

Pyridoxine/pyridoxamine 5'-phosphate oxidase 2

Gene

PPOX2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP). Has an in vitro catalytic efficiency for PNP approximately 300-fold lower than that of PPOX1.1 Publication

Catalytic activityi

Pyridoxamine 5'-phosphate + H2O + O2 = pyridoxal 5'-phosphate + NH3 + H2O2.1 Publication
Pyridoxine 5'-phosphate + O2 = pyridoxal 5'-phosphate + H2O2.1 Publication

Cofactori

FMNBy similarityNote: Binds 1 FMN per subunit.By similarity

Pathwayi: pyridoxal 5'-phosphate salvage

This protein is involved in step 1 of the subpathway that synthesizes pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Pyridoxine/pyridoxamine 5'-phosphate oxidase 2 (PPOX2), Pyridoxine/pyridoxamine 5'-phosphate oxidase 1, chloroplastic (PPOX1)
This subpathway is part of the pathway pyridoxal 5'-phosphate salvage, which is itself part of Cofactor metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate, the pathway pyridoxal 5'-phosphate salvage and in Cofactor metabolism.

Pathwayi: pyridoxal 5'-phosphate salvage

This protein is involved in step 1 of the subpathway that synthesizes pyridoxal 5'-phosphate from pyridoxine 5'-phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Pyridoxine/pyridoxamine 5'-phosphate oxidase 2 (PPOX2), Pyridoxine/pyridoxamine 5'-phosphate oxidase 1, chloroplastic (PPOX1)
This subpathway is part of the pathway pyridoxal 5'-phosphate salvage, which is itself part of Cofactor metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxal 5'-phosphate from pyridoxine 5'-phosphate, the pathway pyridoxal 5'-phosphate salvage and in Cofactor metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei42FMNBy similarity1
Binding sitei66FMNBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi59 – 60FMNBy similarity2
Nucleotide bindingi121 – 122FMNBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processPyridoxine biosynthesis
LigandFlavoprotein, FMN

Enzyme and pathway databases

UniPathwayiUPA01068; UER00304
UPA01068; UER00305

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxine/pyridoxamine 5'-phosphate oxidase 2 (EC:1.4.3.5)
Short name:
AtPPOX1
Gene namesi
Name:PPOX2
Ordered Locus Names:At2g46580
ORF Names:F13A10.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G46580
TAIRilocus:2039949 AT2G46580

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004205501 – 198Pyridoxine/pyridoxamine 5'-phosphate oxidase 2Add BLAST198

Proteomic databases

PaxDbiQ9ZPY1

PTM databases

iPTMnetiQ9ZPY1

Expressioni

Gene expression databases

ExpressionAtlasiQ9ZPY1 baseline and differential
GenevisibleiQ9ZPY1 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT2G46580.1

Structurei

3D structure databases

ProteinModelPortaliQ9ZPY1
SMRiQ9ZPY1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi158 – 161Poly-Leu4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG4558 Eukaryota
COG5135 LUCA
HOGENOMiHOG000233130
OMAiARLQFAW
OrthoDBiEOG09360ODO
PhylomeDBiQ9ZPY1

Family and domain databases

Gene3Di2.30.110.10, 1 hit
InterProiView protein in InterPro
IPR024624 Pyridox_Oxase_Alr4036_FMN-bd
IPR024015 Pyridox_Oxase_FMN-dep_Alr4036
IPR012349 Split_barrel_FMN-bd
PfamiView protein in Pfam
PF12766 Pyridox_oxase_2, 1 hit
TIGRFAMsiTIGR04026 PPOX_FMN_cyano, 1 hit

Sequencei

Sequence statusi: Complete.

Q9ZPY1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTHVAPWKQ LLFGAIEANS HLSHSSYVQL ATIGLNGRPS NRTVVFRGFE
60 70 80 90 100
ENSDRIQINT DLRSRKIEEL KHCPFSEMCW YFSDTWEQFR INGRIEVIDA
110 120 130 140 150
SNPDQTKLQQ REKAWFANSL RSRLIYVCPT PGSPCNSEQS SQQVKLDPSS
160 170 180 190
GPVPEYCLLL LEPEKVDYLN LKTNQRLFFS SMATGTGEKC WTSEKVNP
Length:198
Mass (Da):22,620
Last modified:June 1, 2002 - v2
Checksum:iEFDE8DA20D6A4376
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006418 Genomic DNA Translation: AAD20168.2
CP002685 Genomic DNA Translation: AEC10724.1
AY086412 mRNA Translation: AAM63414.1
BT024917 mRNA Translation: ABD94073.1
PIRiF84904
RefSeqiNP_566081.1, NM_130223.3
UniGeneiAt.21431

Genome annotation databases

EnsemblPlantsiAT2G46580.1; AT2G46580.1; AT2G46580
GeneIDi819270
GrameneiAT2G46580.1; AT2G46580.1; AT2G46580
KEGGiath:AT2G46580

Similar proteinsi

Entry informationi

Entry nameiPPOX2_ARATH
AccessioniPrimary (citable) accession number: Q9ZPY1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: June 1, 2002
Last modified: April 25, 2018
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health