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Q9ZPY1

- PPOX2_ARATH

UniProt

Q9ZPY1 - PPOX2_ARATH

Protein

Pyridoxine/pyridoxamine 5'-phosphate oxidase 2

Gene

PPOX2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 2 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP). Has an in vitro catalytic efficiency for PNP approximately 300-fold lower than that of PPOX1.1 Publication

    Catalytic activityi

    Pyridoxamine 5'-phosphate + H2O + O2 = pyridoxal 5'-phosphate + NH3 + H2O2.1 Publication
    Pyridoxine 5'-phosphate + O2 = pyridoxal 5'-phosphate + H2O2.1 Publication

    Cofactori

    Binds 1 FMN per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei42 – 421FMNBy similarity
    Binding sitei66 – 661FMNBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi59 – 602FMNBy similarity
    Nucleotide bindingi121 – 1222FMNBy similarity

    GO - Molecular functioni

    1. FMN binding Source: InterPro
    2. pyridoxamine-phosphate oxidase activity Source: UniProtKB

    GO - Biological processi

    1. pyridoxal 5'-phosphate salvage Source: UniProtKB
    2. pyridoxine biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Pyridoxine biosynthesis

    Keywords - Ligandi

    Flavoprotein, FMN

    Enzyme and pathway databases

    UniPathwayiUPA00190; UER00304.
    UPA00190; UER00305.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyridoxine/pyridoxamine 5'-phosphate oxidase 2 (EC:1.4.3.5)
    Short name:
    AtPPOX1
    Gene namesi
    Name:PPOX2
    Ordered Locus Names:At2g46580
    ORF Names:F13A10.11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G46580.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 198198Pyridoxine/pyridoxamine 5'-phosphate oxidase 2PRO_0000420550Add
    BLAST

    Proteomic databases

    PaxDbiQ9ZPY1.
    PRIDEiQ9ZPY1.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9ZPY1.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi3702.AT2G46580.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ZPY1.
    SMRiQ9ZPY1. Positions 2-198.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi158 – 1614Poly-Leu

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG5135.
    HOGENOMiHOG000233130.
    InParanoidiQ9ZPY1.
    OMAiNYVEECA.
    PhylomeDBiQ9ZPY1.

    Family and domain databases

    Gene3Di2.30.110.10. 1 hit.
    InterProiIPR000659. Pyridox_Oxase.
    IPR024624. Pyridox_Oxase_Alr4036_FMN-bd.
    IPR024015. Pyridox_Oxase_FMN-dep_Alr4036.
    IPR012349. Split_barrel_FMN-bd.
    [Graphical view]
    PANTHERiPTHR10851. PTHR10851. 1 hit.
    PfamiPF12766. Pyridox_oxase_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF50475. SSF50475. 1 hit.
    TIGRFAMsiTIGR04026. PPOX_FMN_cyano. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9ZPY1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGTHVAPWKQ LLFGAIEANS HLSHSSYVQL ATIGLNGRPS NRTVVFRGFE    50
    ENSDRIQINT DLRSRKIEEL KHCPFSEMCW YFSDTWEQFR INGRIEVIDA 100
    SNPDQTKLQQ REKAWFANSL RSRLIYVCPT PGSPCNSEQS SQQVKLDPSS 150
    GPVPEYCLLL LEPEKVDYLN LKTNQRLFFS SMATGTGEKC WTSEKVNP 198
    Length:198
    Mass (Da):22,620
    Last modified:June 1, 2002 - v2
    Checksum:iEFDE8DA20D6A4376
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006418 Genomic DNA. Translation: AAD20168.2.
    CP002685 Genomic DNA. Translation: AEC10724.1.
    AY086412 mRNA. Translation: AAM63414.1.
    BT024917 mRNA. Translation: ABD94073.1.
    PIRiF84904.
    RefSeqiNP_566081.1. NM_130223.2.
    UniGeneiAt.21431.

    Genome annotation databases

    EnsemblPlantsiAT2G46580.1; AT2G46580.1; AT2G46580.
    GeneIDi819270.
    KEGGiath:AT2G46580.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006418 Genomic DNA. Translation: AAD20168.2 .
    CP002685 Genomic DNA. Translation: AEC10724.1 .
    AY086412 mRNA. Translation: AAM63414.1 .
    BT024917 mRNA. Translation: ABD94073.1 .
    PIRi F84904.
    RefSeqi NP_566081.1. NM_130223.2.
    UniGenei At.21431.

    3D structure databases

    ProteinModelPortali Q9ZPY1.
    SMRi Q9ZPY1. Positions 2-198.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT2G46580.1-P.

    Proteomic databases

    PaxDbi Q9ZPY1.
    PRIDEi Q9ZPY1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G46580.1 ; AT2G46580.1 ; AT2G46580 .
    GeneIDi 819270.
    KEGGi ath:AT2G46580.

    Organism-specific databases

    TAIRi AT2G46580.

    Phylogenomic databases

    eggNOGi COG5135.
    HOGENOMi HOG000233130.
    InParanoidi Q9ZPY1.
    OMAi NYVEECA.
    PhylomeDBi Q9ZPY1.

    Enzyme and pathway databases

    UniPathwayi UPA00190 ; UER00304 .
    UPA00190 ; UER00305 .

    Miscellaneous databases

    PROi Q9ZPY1.

    Gene expression databases

    Genevestigatori Q9ZPY1.

    Family and domain databases

    Gene3Di 2.30.110.10. 1 hit.
    InterProi IPR000659. Pyridox_Oxase.
    IPR024624. Pyridox_Oxase_Alr4036_FMN-bd.
    IPR024015. Pyridox_Oxase_FMN-dep_Alr4036.
    IPR012349. Split_barrel_FMN-bd.
    [Graphical view ]
    PANTHERi PTHR10851. PTHR10851. 1 hit.
    Pfami PF12766. Pyridox_oxase_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50475. SSF50475. 1 hit.
    TIGRFAMsi TIGR04026. PPOX_FMN_cyano. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Arabidopsis ORF clones."
      Shinn P., Chen H., Kim C.J., Ecker J.R.
      Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Identification of a second pyridoxine (pyridoxamine) 50-phosphate oxidase in Arabidopsis thaliana."
      Sang Y., Goertzen L.R., Tzou Y.-M., Locy R.D., Singh N.K.
      Acta Physiol. Plant. 33:559-566(2011)
      Cited for: FUNCTION, CATALYTIC ACTIVITY.

    Entry informationi

    Entry nameiPPOX2_ARATH
    AccessioniPrimary (citable) accession number: Q9ZPY1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 9, 2013
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 94 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3