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Protein

Pyridoxine/pyridoxamine 5'-phosphate oxidase 2

Gene

PPOX2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP). Has an in vitro catalytic efficiency for PNP approximately 300-fold lower than that of PPOX1.1 Publication

Catalytic activityi

Pyridoxamine 5'-phosphate + H2O + O2 = pyridoxal 5'-phosphate + NH3 + H2O2.1 Publication
Pyridoxine 5'-phosphate + O2 = pyridoxal 5'-phosphate + H2O2.1 Publication

Cofactori

FMNBy similarityNote: Binds 1 FMN per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei42 – 421FMNBy similarity
Binding sitei66 – 661FMNBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi59 – 602FMNBy similarity
Nucleotide bindingi121 – 1222FMNBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Pyridoxine biosynthesis

Keywords - Ligandi

Flavoprotein, FMN

Enzyme and pathway databases

UniPathwayiUPA00190; UER00304.
UPA00190; UER00305.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxine/pyridoxamine 5'-phosphate oxidase 2 (EC:1.4.3.5)
Short name:
AtPPOX1
Gene namesi
Name:PPOX2
Ordered Locus Names:At2g46580
ORF Names:F13A10.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G46580.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 198198Pyridoxine/pyridoxamine 5'-phosphate oxidase 2PRO_0000420550Add
BLAST

Proteomic databases

PaxDbiQ9ZPY1.
PRIDEiQ9ZPY1.

Expressioni

Gene expression databases

GenevestigatoriQ9ZPY1.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT2G46580.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9ZPY1.
SMRiQ9ZPY1. Positions 2-198.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi158 – 1614Poly-Leu

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5135.
HOGENOMiHOG000233130.
OMAiRGNPQNR.
PhylomeDBiQ9ZPY1.

Family and domain databases

Gene3Di2.30.110.10. 1 hit.
InterProiIPR000659. Pyridox_Oxase.
IPR024624. Pyridox_Oxase_Alr4036_FMN-bd.
IPR024015. Pyridox_Oxase_FMN-dep_Alr4036.
IPR012349. Split_barrel_FMN-bd.
[Graphical view]
PANTHERiPTHR10851. PTHR10851. 1 hit.
PfamiPF12766. Pyridox_oxase_2. 1 hit.
[Graphical view]
SUPFAMiSSF50475. SSF50475. 1 hit.
TIGRFAMsiTIGR04026. PPOX_FMN_cyano. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9ZPY1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTHVAPWKQ LLFGAIEANS HLSHSSYVQL ATIGLNGRPS NRTVVFRGFE
60 70 80 90 100
ENSDRIQINT DLRSRKIEEL KHCPFSEMCW YFSDTWEQFR INGRIEVIDA
110 120 130 140 150
SNPDQTKLQQ REKAWFANSL RSRLIYVCPT PGSPCNSEQS SQQVKLDPSS
160 170 180 190
GPVPEYCLLL LEPEKVDYLN LKTNQRLFFS SMATGTGEKC WTSEKVNP
Length:198
Mass (Da):22,620
Last modified:June 1, 2002 - v2
Checksum:iEFDE8DA20D6A4376
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006418 Genomic DNA. Translation: AAD20168.2.
CP002685 Genomic DNA. Translation: AEC10724.1.
AY086412 mRNA. Translation: AAM63414.1.
BT024917 mRNA. Translation: ABD94073.1.
PIRiF84904.
RefSeqiNP_566081.1. NM_130223.2.
UniGeneiAt.21431.

Genome annotation databases

EnsemblPlantsiAT2G46580.1; AT2G46580.1; AT2G46580.
GeneIDi819270.
KEGGiath:AT2G46580.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006418 Genomic DNA. Translation: AAD20168.2.
CP002685 Genomic DNA. Translation: AEC10724.1.
AY086412 mRNA. Translation: AAM63414.1.
BT024917 mRNA. Translation: ABD94073.1.
PIRiF84904.
RefSeqiNP_566081.1. NM_130223.2.
UniGeneiAt.21431.

3D structure databases

ProteinModelPortaliQ9ZPY1.
SMRiQ9ZPY1. Positions 2-198.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G46580.1-P.

Proteomic databases

PaxDbiQ9ZPY1.
PRIDEiQ9ZPY1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G46580.1; AT2G46580.1; AT2G46580.
GeneIDi819270.
KEGGiath:AT2G46580.

Organism-specific databases

TAIRiAT2G46580.

Phylogenomic databases

eggNOGiCOG5135.
HOGENOMiHOG000233130.
OMAiRGNPQNR.
PhylomeDBiQ9ZPY1.

Enzyme and pathway databases

UniPathwayiUPA00190; UER00304.
UPA00190; UER00305.

Miscellaneous databases

PROiQ9ZPY1.

Gene expression databases

GenevestigatoriQ9ZPY1.

Family and domain databases

Gene3Di2.30.110.10. 1 hit.
InterProiIPR000659. Pyridox_Oxase.
IPR024624. Pyridox_Oxase_Alr4036_FMN-bd.
IPR024015. Pyridox_Oxase_FMN-dep_Alr4036.
IPR012349. Split_barrel_FMN-bd.
[Graphical view]
PANTHERiPTHR10851. PTHR10851. 1 hit.
PfamiPF12766. Pyridox_oxase_2. 1 hit.
[Graphical view]
SUPFAMiSSF50475. SSF50475. 1 hit.
TIGRFAMsiTIGR04026. PPOX_FMN_cyano. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Arabidopsis ORF clones."
    Shinn P., Chen H., Kim C.J., Ecker J.R.
    Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Identification of a second pyridoxine (pyridoxamine) 50-phosphate oxidase in Arabidopsis thaliana."
    Sang Y., Goertzen L.R., Tzou Y.-M., Locy R.D., Singh N.K.
    Acta Physiol. Plant. 33:559-566(2011)
    Cited for: FUNCTION, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiPPOX2_ARATH
AccessioniPrimary (citable) accession number: Q9ZPY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: June 1, 2002
Last modified: January 7, 2015
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.