Q9ZPY1 (PPOX2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyridoxine/pyridoxamine 5'-phosphate oxidase 2
Short name=AtPPOX1
EC=1.4.3.5

Gene names

Name:	PPOX2
Ordered Locus Names:	At2g46580
ORF Names:	F13A10.11

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP). Has an in vitro catalytic efficiency for PNP approximately 300-fold lower than that of PPOX1. Ref.5
Catalytic activity	Pyridoxamine 5'-phosphate + H2O + O2 = pyridoxal 5'-phosphate + NH3 + H2O2. Ref.5 Pyridoxine 5'-phosphate + O2 = pyridoxal 5'-phosphate + H2O2. Ref.5
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Cofactor biosynthesis; B6 vitamer interconversion; pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate: step 1/1. Cofactor biosynthesis; B6 vitamer interconversion; pyridoxal 5'-phosphate from pyridoxine 5'-phosphate: step 1/1.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the pyridoxamine 5'-phosphate oxidase family.

Ontologies

Keywords
Biological process	Pyridoxine biosynthesis
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pyridoxal 5'-phosphate salvage Inferred from direct assay. Source: UniProtKB pyridoxine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	FMN binding Inferred from electronic annotation. Source: InterPro pyridoxamine-phosphate oxidase activity Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 198	198	Pyridoxine/pyridoxamine 5'-phosphate oxidase 2		PRO_0000420550
Regions
	Nucleotide binding	59 – 60	2	FMN By similarity
	Nucleotide binding	121 – 122	2	FMN By similarity
	Compositional bias	158 – 161	4	Poly-Leu
Sites
	Binding site	42	1	FMN By similarity
	Binding site	66	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZPY1 [UniParc]. Last modified June 1, 2002. Version 2. Checksum: EFDE8DA20D6A4376	FASTA	198	22,620
	10 20 30 40 50 60 MGTHVAPWKQ LLFGAIEANS HLSHSSYVQL ATIGLNGRPS NRTVVFRGFE ENSDRIQINT 70 80 90 100 110 120 DLRSRKIEEL KHCPFSEMCW YFSDTWEQFR INGRIEVIDA SNPDQTKLQQ REKAWFANSL 130 140 150 160 170 180 RSRLIYVCPT PGSPCNSEQS SQQVKLDPSS GPVPEYCLLL LEPEKVDYLN LKTNQRLFFS 190 SMATGTGEKC WTSEKVNP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Arabidopsis ORF clones." Shinn P., Chen H., Kim C.J., Ecker J.R. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Identification of a second pyridoxine (pyridoxamine) 50-phosphate oxidase in Arabidopsis thaliana." Sang Y., Goertzen L.R., Tzou Y.-M., Locy R.D., Singh N.K. Acta Physiol. Plant. 33:559-566(2011) Cited for: FUNCTION, CATALYTIC ACTIVITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC006418 Genomic DNA. Translation: AAD20168.2. CP002685 Genomic DNA. Translation: AEC10724.1. AY086412 mRNA. Translation: AAM63414.1. BT024917 mRNA. Translation: ABD94073.1.
IPI	IPI00540909.
PIR	F84904.
RefSeq	NP_566081.1. NM_130223.2.
UniGene	At.21431.
3D structure databases
ProteinModelPortal	Q9ZPY1.
SMR	Q9ZPY1. Positions 2-198.
ModBase	Search...
Proteomic databases
PaxDb	Q9ZPY1.
PRIDE	Q9ZPY1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G46580.1; AT2G46580.1; AT2G46580.
GeneID	819270.
KEGG	ath:AT2G46580.
Organism-specific databases
TAIR	At2g46580.
Phylogenomic databases
eggNOG	COG5135.
HOGENOM	HOG000233130.
InParanoid	Q9ZPY1.
OMA	ALHRNRS.
PhylomeDB	Q9ZPY1.
ProtClustDB	CLSN2688969.
Enzyme and pathway databases
UniPathway	UPA00190; UER00304. UPA00190; UER00305.
Gene expression databases
Genevestigator	Q9ZPY1.
Family and domain databases
Gene3D	2.30.110.10. 1 hit.
InterPro	IPR000659. Pyridox_Oxase. IPR024624. Pyridox_Oxase_Alr4036_FMN-bd. IPR024015. Pyridox_Oxase_FMN-dep_Alr4036. IPR012349. Split_barrel_FMN-bd. [Graphical view]
PANTHER	PTHR10851. PTHR10851. 1 hit.
Pfam	PF12766. Pyridox_oxase_2. 1 hit. [Graphical view]
SUPFAM	SSF50475. FMN_binding. 1 hit.
TIGRFAMs	TIGR04026. PPOX_FMN_cyano. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPOX2_ARATH

Accession

Primary (citable) accession number: Q9ZPY1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2013
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program