Q9ZPX5 (DHSA2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Succinate dehydrogenase [ubiquinone] flavoprotein subunit 2, mitochondrial
EC=1.3.5.1

Alternative name(s):
Flavoprotein subunit 2 of complex II
Short name=FP

Gene names

Name:	SDH1-2
Ordered Locus Names:	At2g18450
ORF Names:	T30D6.4

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	632 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) By similarity.
Catalytic activity	Succinate + ubiquinone = fumarate + ubiquinol.
Cofactor	FAD By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.
Subunit structure	Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit By similarity. Ref.5
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side Ref.6.
Tissue specificity	Expressed at a low level. Ref.5
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mitochondrial electron transport, succinate to ubiquinone Traceable author statement Ref.5. Source: TAIR transport Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro succinate dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 29	29	Mitochondrion Potential
	Chain	30 – 632	603	Succinate dehydrogenase [ubiquinone] flavoprotein subunit 2, mitochondrial		PRO_0000247592
Regions
	Nucleotide binding	54 – 59	6	FAD By similarity
	Nucleotide binding	77 – 92	16	FAD By similarity
	Nucleotide binding	444 – 445	2	FAD By similarity
Sites
	Active site	327	1	Proton acceptor By similarity
	Binding site	262	1	FAD By similarity
	Binding site	283	1	Substrate By similarity
	Binding site	295	1	Substrate By similarity
	Binding site	394	1	Substrate By similarity
	Binding site	428	1	FAD By similarity
	Binding site	439	1	Substrate By similarity
Amino acid modifications
	Modified residue	85	1	Tele-8alpha-FAD histidine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZPX5 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: DEC34651679CEF88	FASTA	632	69,363
	10 20 30 40 50 60 MWRCLRVASS SRRSESNGAF ITSQLSRFFS APPSAGDKSS YTIVDHTYDA VVVGAGGAGL 70 80 90 100 110 120 RAAIGLSEHG FNTACITKLF PTRSHTVAAQ GGINAALGNM SVDDWRWHMY DTVKGSDWLG 130 140 150 160 170 180 DQDAIQYMCR EAPKAVIELE NYGLPFSRTE DGKIYQRAFG GQSLEFGIGG QAYRCACAAD 190 200 210 220 230 240 RTGHALLHTL YGQAMKHNTQ FFVEYFALDL IMNSDGTCQG VIALNMEDGT LHRFHAGSTI 250 260 270 280 290 300 LATGGYGRAY FSATSAHTCT GDGNAMVARA GLPLQDLEFV QFHPTGIYGA GCLITEGARG 310 320 330 340 350 360 EGGILRNSEG EKFMDRYAPT ARDLASRDVV SRSMTMEIRQ GRGAGPMKDY LYLYLNHLPP 370 380 390 400 410 420 EVLKERLPGI SETAAIFAGV DVTREPIPVL PTVHYNMGGI PTNYHGEVIT LRGDDPDAVV 430 440 450 460 470 480 PGLMAAGEAA CASVHGANRL GANSLLDIVV FGRACANRVA EIQKPGEKLK PLEKDAGEKS 490 500 510 520 530 540 IEWLDRIRNS NGSLPTSKIR LNMQRVMQNN AAVFRTQETL EEGCDLIDKT WDSFGDVKVT 550 560 570 580 590 600 DRSMIWNSDL IETMELENLL VNACITMHSA EARKESRGAH AREDFTKRDD ANWMKHTLGY 610 620 630 WEEGNVKLEY RPVHMKTLDD EVDTFPPKPR VY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed: 11910074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"The four subunits of mitochondrial respiratory complex II are encoded by multiple nuclear genes and targeted to mitochondria in Arabidopsis thaliana." Figueroa P., Leon G., Elorza A., Holuigue L., Araya A., Jordana X. Plant Mol. Biol. 50:725-734(2002) [PubMed: 12374303] [Abstract] Cited for: SUBUNIT, TISSUE SPECIFICITY.
[6]	"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed: 14671022] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. Strain: cv. Landsberg erecta.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC006439 Genomic DNA. Translation: AAD15493.1. CP002685 Genomic DNA. Translation: AEC06769.1. AK119142 mRNA. Translation: BAC43712.1. BT005938 mRNA. Translation: AAO64873.1.
IPI	IPI00527700.
PIR	D84564.
RefSeq	NP_179435.1. NM_127401.2.
UniGene	At.39995.
3D structure databases
ProteinModelPortal	Q9ZPX5.
SMR	Q9ZPX5. Positions 39-632.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9ZPX5.
Proteomic databases
PRIDE	Q9ZPX5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G18450.1; AT2G18450.1; AT2G18450.
GeneID	816359.
GenomeReviews	Gene locus AT2G18450 in contig CT485783_GR.
KEGG	ath:AT2G18450.
NMPDR	fig|3702.1.peg.8841.
Organism-specific databases
GeneFarm	2186. 174.
TAIR	At2g18450.
Phylogenomic databases
eggNOG	KOG2403.
GeneTree	EPGT00070000038628.
HOGENOM	HBG293998.
InParanoid	Q9ZPX5.
OMA	GRDDENW.
PhylomeDB	Q9ZPX5.
ProtClustDB	PLN00128.
Gene expression databases
ArrayExpress	Q9ZPX5.
Genevestigator	Q9ZPX5.
GermOnline	AT2G18450. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR003952. FRD_SDH_FAD_BS. IPR015939. Fum_Rdtase/Succ_DH_flav-like_C. IPR004112. Fum_Rdtase/Succ_DH_flav_C. IPR011281. Succ_DH_flav_su_fwd. IPR014006. Succ_Dhase_FrdA_Gneg. [Graphical view]
Gene3D	G3DSA:1.20.58.100. Fum_Rdtase/Succ_DH_flav-like_C. 1 hit.
KO	K00234.
PANTHER	PTHR11632:SF5. PTHR11632:SF5. 1 hit.
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
SUPFAM	SSF46977. Succ_DH_flav_C. 1 hit.
TIGRFAMs	TIGR01816. SdhA_forward. 1 hit. TIGR01812. SdhA_frdA_Gneg. 1 hit.
PROSITE	PS00504. FRD_SDH_FAD_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHSA2_ARATH

Accession

Primary (citable) accession number: Q9ZPX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	May 1, 1999
Last modified:	November 16, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program