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Protein

Phosphopantetheine adenylyltransferase

Gene

COAD

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate. Does not accept 4'-phosphopantothenoylcysteine as a substrate.2 Publications

Catalytic activityi

ATP + pantetheine 4'-phosphate = diphosphate + 3'-dephospho-CoA.

Enzyme regulationi

Inhibited by CoA.1 Publication

Kineticsi

  1. KM=37 µM for 3'-dephospho-CoA1 Publication
  1. Vmax=0.34 µmol/min/mg enzyme for the reverse reaction1 Publication

Pathwayi: coenzyme A biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes CoA from (R)-pantothenate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pantothenate kinase 2 (PANK2), Pantothenate kinase 1 (PANK1)
  2. Phosphopantothenate--cysteine ligase 2 (PPCS2), Phosphopantothenate--cysteine ligase 1 (PPCS1)
  3. Probable phosphopantothenoylcysteine decarboxylase (HAL3B), Phosphopantothenoylcysteine decarboxylase (HAL3A)
  4. Phosphopantetheine adenylyltransferase (COAD)
  5. Dephospho-CoA kinase (COAE)
This subpathway is part of the pathway coenzyme A biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CoA from (R)-pantothenate, the pathway coenzyme A biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • coenzyme A biosynthetic process Source: TAIR
  • growth Source: TAIR
  • lipid metabolic process Source: TAIR
  • lipid storage Source: TAIR
  • regulation of coenzyme A biosynthetic process Source: TAIR
  • response to osmotic stress Source: TAIR
  • response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Coenzyme A biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKQ9ZPV8.
UniPathwayiUPA00241; UER00355.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphopantetheine adenylyltransferase (EC:2.7.7.3)
Alternative name(s):
AtCoaD
Dephospho-CoA pyrophosphorylase
Pantetheine-phosphate adenylyltransferase
Gene namesi
Name:COAD
Synonyms:PPAT
Ordered Locus Names:At2g18250
ORF Names:T30D6.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G18250.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Plants are severely impaired in plant growth and seed production, but almost not affected in the accumulation of total fatty acids per seed.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003508161 – 176Phosphopantetheine adenylyltransferaseAdd BLAST176

Proteomic databases

PaxDbiQ9ZPV8.

Expressioni

Gene expression databases

GenevisibleiQ9ZPV8. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G18250.1.

Structurei

3D structure databases

ProteinModelPortaliQ9ZPV8.
SMRiQ9ZPV8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the eukaryotic CoaD family.Curated

Phylogenomic databases

eggNOGiKOG3351. Eukaryota.
COG1019. LUCA.
HOGENOMiHOG000284155.
InParanoidiQ9ZPV8.
KOiK02201.
OMAiLQINDER.
OrthoDBiEOG09360QGQ.
PhylomeDBiQ9ZPV8.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR004821. Cyt_trans-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00125. cyt_tran_rel. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9ZPV8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPEDSKMS PANSFGAVVL GGTFDRLHDG HRMFLKAAAE LARDRIVVGV
60 70 80 90 100
CDGPMLTKKQ FSDMIQPIEE RMRNVETYVK SIKPELVVQA EPITDPYGPS
110 120 130 140 150
IVDENLEAIV VSKETLPGGL SVNRKRAERG LSQLKIEVVE IVSDGSSGNK
160 170
ISSSTLRKME AEKASKQKQP AEEKAS
Length:176
Mass (Da):19,168
Last modified:May 1, 1999 - v1
Checksum:iFE0E3F58FC8B9518
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006439 Genomic DNA. Translation: AAD15511.1.
CP002685 Genomic DNA. Translation: AEC06746.1.
BT010494 mRNA. Translation: AAQ65117.1.
AK176897 mRNA. Translation: BAD44660.1.
PIRiB84562.
RefSeqiNP_179417.1. NM_127383.3.
UniGeneiAt.40020.

Genome annotation databases

EnsemblPlantsiAT2G18250.1; AT2G18250.1; AT2G18250.
GeneIDi816341.
GrameneiAT2G18250.1; AT2G18250.1; AT2G18250.
KEGGiath:AT2G18250.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006439 Genomic DNA. Translation: AAD15511.1.
CP002685 Genomic DNA. Translation: AEC06746.1.
BT010494 mRNA. Translation: AAQ65117.1.
AK176897 mRNA. Translation: BAD44660.1.
PIRiB84562.
RefSeqiNP_179417.1. NM_127383.3.
UniGeneiAt.40020.

3D structure databases

ProteinModelPortaliQ9ZPV8.
SMRiQ9ZPV8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G18250.1.

Proteomic databases

PaxDbiQ9ZPV8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G18250.1; AT2G18250.1; AT2G18250.
GeneIDi816341.
GrameneiAT2G18250.1; AT2G18250.1; AT2G18250.
KEGGiath:AT2G18250.

Organism-specific databases

TAIRiAT2G18250.

Phylogenomic databases

eggNOGiKOG3351. Eukaryota.
COG1019. LUCA.
HOGENOMiHOG000284155.
InParanoidiQ9ZPV8.
KOiK02201.
OMAiLQINDER.
OrthoDBiEOG09360QGQ.
PhylomeDBiQ9ZPV8.

Enzyme and pathway databases

UniPathwayiUPA00241; UER00355.
SABIO-RKQ9ZPV8.

Miscellaneous databases

PROiQ9ZPV8.

Gene expression databases

GenevisibleiQ9ZPV8. AT.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR004821. Cyt_trans-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00125. cyt_tran_rel. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCOAD_ARATH
AccessioniPrimary (citable) accession number: Q9ZPV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 23, 2008
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.