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Protein

Serine/threonine-protein kinase BRI1-like 2

Gene

BRL2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor with a serine/threonine-protein kinase activity, which may transduce extracellular spatial and temporal signals into downstream cell differentiation responses in provascular and procambial cells. In contrast to BRI1, BRL1 and BRL3, it does not bind brassinolide.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei866ATPPROSITE-ProRule annotation1
Active sitei966Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi844 – 852ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • transmembrane receptor protein serine/threonine kinase activity Source: TAIR

GO - Biological processi

  • auxin-activated signaling pathway Source: TAIR
  • brassinosteroid mediated signaling pathway Source: TAIR
  • leaf vascular tissue pattern formation Source: TAIR
  • phloem transport Source: TAIR
  • xylem and phloem pattern formation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase BRI1-like 2 (EC:2.7.11.1)
Alternative name(s):
BRASSINOSTEROID INSENSITIVE 1-like protein 2
Protein VASCULAR HIGHWAY 1
Gene namesi
Name:BRL2
Synonyms:VH1
Ordered Locus Names:At2g01950
ORF Names:F14H20.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G01950.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini32 – 756ExtracellularSequence analysisAdd BLAST725
Transmembranei757 – 777HelicalSequence analysisAdd BLAST21
Topological domaini778 – 1143CytoplasmicSequence analysisAdd BLAST366

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
ChainiPRO_000002430932 – 1143Serine/threonine-protein kinase BRI1-like 2Add BLAST1112

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi84N-linked (GlcNAc...)Sequence analysis1
Glycosylationi118N-linked (GlcNAc...)Sequence analysis1
Glycosylationi163N-linked (GlcNAc...)Sequence analysis1
Glycosylationi188N-linked (GlcNAc...)Sequence analysis1
Glycosylationi226N-linked (GlcNAc...)Sequence analysis1
Glycosylationi234N-linked (GlcNAc...)Sequence analysis1
Glycosylationi288N-linked (GlcNAc...)Sequence analysis1
Glycosylationi312N-linked (GlcNAc...)Sequence analysis1
Glycosylationi412N-linked (GlcNAc...)Sequence analysis1
Glycosylationi469N-linked (GlcNAc...)Sequence analysis1
Glycosylationi506N-linked (GlcNAc...)Sequence analysis1
Glycosylationi681N-linked (GlcNAc...)Sequence analysis1
Modified residuei835PhosphothreonineBy similarity1
Modified residuei911PhosphotyrosineBy similarity1
Modified residuei1001PhosphoserineBy similarity1
Modified residuei1009PhosphotyrosineBy similarity1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9ZPS9.

Expressioni

Tissue specificityi

Expressed in provascular and procambial sites throughout plant development. Expressed throughout globe- to heart-staged embryos. Then, it is restricted to procambial cells by the late torpedo stage, and this pattern persists throughout the duration of embryo development. After germination, it is expressed not only in procambial cells throughout the plant but also in all lateral organ primordia before the onset of vascularization.2 Publications

Gene expression databases

ExpressionAtlasiQ9ZPS9. baseline and differential.
GenevisibleiQ9ZPS9. AT.

Interactioni

Subunit structurei

Interacts with TTL3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
F7A19.9Q9XI872EBI-2292728,EBI-2292778
TTL3Q9SIN12EBI-2292728,EBI-2292882

Protein-protein interaction databases

BioGridi129. 68 interactors.
IntActiQ9ZPS9. 64 interactors.
MINTiMINT-8064047.
STRINGi3702.AT2G01950.1.

Structurei

3D structure databases

ProteinModelPortaliQ9ZPS9.
SMRiQ9ZPS9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati79 – 100LRR 1Add BLAST22
Repeati104 – 126LRR 2Add BLAST23
Repeati128 – 150LRR 3Add BLAST23
Repeati153 – 175LRR 4Add BLAST23
Repeati178 – 200LRR 5Add BLAST23
Repeati205 – 228LRR 6Add BLAST24
Repeati229 – 251LRR 7Add BLAST23
Repeati253 – 275LRR 8Add BLAST23
Repeati278 – 299LRR 9Add BLAST22
Repeati302 – 323LRR 10Add BLAST22
Repeati327 – 349LRR 11Add BLAST23
Repeati351 – 372LRR 12Add BLAST22
Repeati376 – 398LRR 13Add BLAST23
Repeati400 – 422LRR 14Add BLAST23
Repeati424 – 446LRR 15Add BLAST23
Repeati448 – 470LRR 16Add BLAST23
Repeati472 – 494LRR 17Add BLAST23
Repeati496 – 518LRR 18Add BLAST23
Repeati520 – 542LRR 19Add BLAST23
Repeati612 – 634LRR 20Add BLAST23
Repeati636 – 658LRR 21Add BLAST23
Repeati660 – 682LRR 22Add BLAST23
Repeati684 – 706LRR 23Add BLAST23
Domaini838 – 1129Protein kinasePROSITE-ProRule annotationAdd BLAST292

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi68 – 75Cys pair 18
Motifi720 – 727Cys pair 28

Domaini

Contains two pairs of conservatively spaced Cys (Cys pair 1 and 2) possibly involved in forming some heterodimers.By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 23 LRR (leucine-rich) repeats.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IE1Y. Eukaryota.
COG4886. LUCA.
HOGENOMiHOG000116551.
InParanoidiQ9ZPS9.
OMAiMEVIDED.
OrthoDBiEOG093600TU.
PhylomeDBiQ9ZPS9.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
3.80.10.10. 4 hits.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR013210. LRR_N_plant-typ.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13855. LRR_8. 3 hits.
PF08263. LRRNT_2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 5 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 3 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51450. LRR. 17 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ZPS9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTSPIRVRI RTRIQISFIF LLTHLSQSSS SDQSSLKTDS LSLLSFKTMI
60 70 80 90 100
QDDPNNILSN WSPRKSPCQF SGVTCLGGRV TEINLSGSGL SGIVSFNAFT
110 120 130 140 150
SLDSLSVLKL SENFFVLNST SLLLLPLTLT HLELSSSGLI GTLPENFFSK
160 170 180 190 200
YSNLISITLS YNNFTGKLPN DLFLSSKKLQ TLDLSYNNIT GPISGLTIPL
210 220 230 240 250
SSCVSMTYLD FSGNSISGYI SDSLINCTNL KSLNLSYNNF DGQIPKSFGE
260 270 280 290 300
LKLLQSLDLS HNRLTGWIPP EIGDTCRSLQ NLRLSYNNFT GVIPESLSSC
310 320 330 340 350
SWLQSLDLSN NNISGPFPNT ILRSFGSLQI LLLSNNLISG DFPTSISACK
360 370 380 390 400
SLRIADFSSN RFSGVIPPDL CPGAASLEEL RLPDNLVTGE IPPAISQCSE
410 420 430 440 450
LRTIDLSLNY LNGTIPPEIG NLQKLEQFIA WYNNIAGEIP PEIGKLQNLK
460 470 480 490 500
DLILNNNQLT GEIPPEFFNC SNIEWVSFTS NRLTGEVPKD FGILSRLAVL
510 520 530 540 550
QLGNNNFTGE IPPELGKCTT LVWLDLNTNH LTGEIPPRLG RQPGSKALSG
560 570 580 590 600
LLSGNTMAFV RNVGNSCKGV GGLVEFSGIR PERLLQIPSL KSCDFTRMYS
610 620 630 640 650
GPILSLFTRY QTIEYLDLSY NQLRGKIPDE IGEMIALQVL ELSHNQLSGE
660 670 680 690 700
IPFTIGQLKN LGVFDASDNR LQGQIPESFS NLSFLVQIDL SNNELTGPIP
710 720 730 740 750
QRGQLSTLPA TQYANNPGLC GVPLPECKNG NNQLPAGTEE GKRAKHGTRA
760 770 780 790 800
ASWANSIVLG VLISAASVCI LIVWAIAVRA RRRDADDAKM LHSLQAVNSA
810 820 830 840 850
TTWKIEKEKE PLSINVATFQ RQLRKLKFSQ LIEATNGFSA ASMIGHGGFG
860 870 880 890 900
EVFKATLKDG SSVAIKKLIR LSCQGDREFM AEMETLGKIK HRNLVPLLGY
910 920 930 940 950
CKIGEERLLV YEFMQYGSLE EVLHGPRTGE KRRILGWEER KKIAKGAAKG
960 970 980 990 1000
LCFLHHNCIP HIIHRDMKSS NVLLDQDMEA RVSDFGMARL ISALDTHLSV
1010 1020 1030 1040 1050
STLAGTPGYV PPEYYQSFRC TAKGDVYSIG VVMLEILSGK RPTDKEEFGD
1060 1070 1080 1090 1100
TNLVGWSKMK AREGKHMEVI DEDLLKEGSS ESLNEKEGFE GGVIVKEMLR
1110 1120 1130 1140
YLEIALRCVD DFPSKRPNML QVVASLRELR GSENNSHSHS NSL
Length:1,143
Mass (Da):125,676
Last modified:May 1, 1999 - v1
Checksum:i7D1C88493F27A94E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006532 Genomic DNA. Translation: AAD20088.1.
CP002685 Genomic DNA. Translation: AEC05526.1.
AY074313 mRNA. Translation: AAL67010.1.
PIRiB84431.
RefSeqiNP_178304.1. NM_126256.4.
UniGeneiAt.13922.

Genome annotation databases

EnsemblPlantsiAT2G01950.1; AT2G01950.1; AT2G01950.
GeneIDi814726.
GrameneiAT2G01950.1; AT2G01950.1; AT2G01950.
KEGGiath:AT2G01950.

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006532 Genomic DNA. Translation: AAD20088.1.
CP002685 Genomic DNA. Translation: AEC05526.1.
AY074313 mRNA. Translation: AAL67010.1.
PIRiB84431.
RefSeqiNP_178304.1. NM_126256.4.
UniGeneiAt.13922.

3D structure databases

ProteinModelPortaliQ9ZPS9.
SMRiQ9ZPS9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi129. 68 interactors.
IntActiQ9ZPS9. 64 interactors.
MINTiMINT-8064047.
STRINGi3702.AT2G01950.1.

Proteomic databases

PaxDbiQ9ZPS9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G01950.1; AT2G01950.1; AT2G01950.
GeneIDi814726.
GrameneiAT2G01950.1; AT2G01950.1; AT2G01950.
KEGGiath:AT2G01950.

Organism-specific databases

TAIRiAT2G01950.

Phylogenomic databases

eggNOGiENOG410IE1Y. Eukaryota.
COG4886. LUCA.
HOGENOMiHOG000116551.
InParanoidiQ9ZPS9.
OMAiMEVIDED.
OrthoDBiEOG093600TU.
PhylomeDBiQ9ZPS9.

Miscellaneous databases

PROiQ9ZPS9.

Gene expression databases

ExpressionAtlasiQ9ZPS9. baseline and differential.
GenevisibleiQ9ZPS9. AT.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
3.80.10.10. 4 hits.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR013210. LRR_N_plant-typ.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13855. LRR_8. 3 hits.
PF08263. LRRNT_2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 5 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 3 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51450. LRR. 17 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBRL2_ARATH
AccessioniPrimary (citable) accession number: Q9ZPS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.