Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9ZPS9

- BRL2_ARATH

UniProt

Q9ZPS9 - BRL2_ARATH

Protein

Serine/threonine-protein kinase BRI1-like 2

Gene

BRL2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Receptor with a serine/threonine-protein kinase activity, which may transduce extracellular spatial and temporal signals into downstream cell differentiation responses in provascular and procambial cells. In contrast to BRI1, BRL1 and BRL3, it does not bind brassinolide.2 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei866 – 8661ATPPROSITE-ProRule annotation
    Active sitei966 – 9661Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi844 – 8529ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. transmembrane receptor protein serine/threonine kinase activity Source: TAIR

    GO - Biological processi

    1. auxin-activated signaling pathway Source: TAIR
    2. brassinosteroid mediated signaling pathway Source: TAIR
    3. leaf vascular tissue pattern formation Source: TAIR
    4. phloem transport Source: TAIR
    5. transmembrane receptor protein serine/threonine kinase signaling pathway Source: GOC
    6. xylem and phloem pattern formation Source: TAIR

    Keywords - Molecular functioni

    Kinase, Receptor, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G01950-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase BRI1-like 2 (EC:2.7.11.1)
    Alternative name(s):
    BRASSINOSTEROID INSENSITIVE 1-like protein 2
    Protein VASCULAR HIGHWAY 1
    Gene namesi
    Name:BRL2
    Synonyms:VH1
    Ordered Locus Names:At2g01950
    ORF Names:F14H20.2
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G01950.

    Subcellular locationi

    Cell membrane 1 Publication; Single-pass type I membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131Sequence AnalysisAdd
    BLAST
    Chaini32 – 11431112Serine/threonine-protein kinase BRI1-like 2PRO_0000024309Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi84 – 841N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi118 – 1181N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi163 – 1631N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi188 – 1881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi226 – 2261N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi234 – 2341N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi312 – 3121N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi412 – 4121N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi469 – 4691N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi506 – 5061N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi681 – 6811N-linked (GlcNAc...)Sequence Analysis
    Modified residuei835 – 8351PhosphothreonineBy similarity
    Modified residuei911 – 9111PhosphotyrosineBy similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ9ZPS9.
    PRIDEiQ9ZPS9.

    Expressioni

    Tissue specificityi

    Expressed in provascular and procambial sites throughout plant development. Expressed throughout globe- to heart-staged embryos. Then, it is restricted to procambial cells by the late torpedo stage, and this pattern persists throughout the duration of embryo development. After germination, it is expressed not only in procambial cells throughout the plant but also in all lateral organ primordia before the onset of vascularization.2 Publications

    Gene expression databases

    ArrayExpressiQ9ZPS9.
    GenevestigatoriQ9ZPS9.

    Interactioni

    Subunit structurei

    Interacts with TTL3.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    F7A19.9Q9XI872EBI-2292728,EBI-2292778
    TTL3Q9SIN12EBI-2292728,EBI-2292882

    Protein-protein interaction databases

    IntActiQ9ZPS9. 64 interactions.
    MINTiMINT-8064047.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ZPS9.
    SMRiQ9ZPS9. Positions 35-727, 790-1131.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini32 – 756725ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini778 – 1143366CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei757 – 77721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati79 – 10022LRR 1Add
    BLAST
    Repeati104 – 12623LRR 2Add
    BLAST
    Repeati128 – 15023LRR 3Add
    BLAST
    Repeati153 – 17523LRR 4Add
    BLAST
    Repeati178 – 20023LRR 5Add
    BLAST
    Repeati205 – 22824LRR 6Add
    BLAST
    Repeati229 – 25123LRR 7Add
    BLAST
    Repeati253 – 27523LRR 8Add
    BLAST
    Repeati278 – 29922LRR 9Add
    BLAST
    Repeati302 – 32322LRR 10Add
    BLAST
    Repeati327 – 34923LRR 11Add
    BLAST
    Repeati351 – 37222LRR 12Add
    BLAST
    Repeati376 – 39823LRR 13Add
    BLAST
    Repeati400 – 42223LRR 14Add
    BLAST
    Repeati424 – 44623LRR 15Add
    BLAST
    Repeati448 – 47023LRR 16Add
    BLAST
    Repeati472 – 49423LRR 17Add
    BLAST
    Repeati496 – 51823LRR 18Add
    BLAST
    Repeati520 – 54223LRR 19Add
    BLAST
    Repeati612 – 63423LRR 20Add
    BLAST
    Repeati636 – 65823LRR 21Add
    BLAST
    Repeati660 – 68223LRR 22Add
    BLAST
    Repeati684 – 70623LRR 23Add
    BLAST
    Domaini838 – 1129292Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi68 – 758Cys pair 1
    Motifi720 – 7278Cys pair 2

    Domaini

    Contains two pairs of conservatively spaced Cys (Cys pair 1 and 2) possibly involved in forming some heterodimers.By similarity

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
    Contains 23 LRR (leucine-rich) repeats.Curated
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG4886.
    HOGENOMiHOG000116551.
    InParanoidiQ9ZPS9.
    OMAiAASWANS.
    PhylomeDBiQ9ZPS9.

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR013320. ConA-like_subgrp.
    IPR011009. Kinase-like_dom.
    IPR001611. Leu-rich_rpt.
    IPR013210. LRR-contain_N2.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00560. LRR_1. 3 hits.
    PF13855. LRR_8. 2 hits.
    PF08263. LRRNT_2. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS51450. LRR. 17 hits.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9ZPS9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTSPIRVRI RTRIQISFIF LLTHLSQSSS SDQSSLKTDS LSLLSFKTMI     50
    QDDPNNILSN WSPRKSPCQF SGVTCLGGRV TEINLSGSGL SGIVSFNAFT 100
    SLDSLSVLKL SENFFVLNST SLLLLPLTLT HLELSSSGLI GTLPENFFSK 150
    YSNLISITLS YNNFTGKLPN DLFLSSKKLQ TLDLSYNNIT GPISGLTIPL 200
    SSCVSMTYLD FSGNSISGYI SDSLINCTNL KSLNLSYNNF DGQIPKSFGE 250
    LKLLQSLDLS HNRLTGWIPP EIGDTCRSLQ NLRLSYNNFT GVIPESLSSC 300
    SWLQSLDLSN NNISGPFPNT ILRSFGSLQI LLLSNNLISG DFPTSISACK 350
    SLRIADFSSN RFSGVIPPDL CPGAASLEEL RLPDNLVTGE IPPAISQCSE 400
    LRTIDLSLNY LNGTIPPEIG NLQKLEQFIA WYNNIAGEIP PEIGKLQNLK 450
    DLILNNNQLT GEIPPEFFNC SNIEWVSFTS NRLTGEVPKD FGILSRLAVL 500
    QLGNNNFTGE IPPELGKCTT LVWLDLNTNH LTGEIPPRLG RQPGSKALSG 550
    LLSGNTMAFV RNVGNSCKGV GGLVEFSGIR PERLLQIPSL KSCDFTRMYS 600
    GPILSLFTRY QTIEYLDLSY NQLRGKIPDE IGEMIALQVL ELSHNQLSGE 650
    IPFTIGQLKN LGVFDASDNR LQGQIPESFS NLSFLVQIDL SNNELTGPIP 700
    QRGQLSTLPA TQYANNPGLC GVPLPECKNG NNQLPAGTEE GKRAKHGTRA 750
    ASWANSIVLG VLISAASVCI LIVWAIAVRA RRRDADDAKM LHSLQAVNSA 800
    TTWKIEKEKE PLSINVATFQ RQLRKLKFSQ LIEATNGFSA ASMIGHGGFG 850
    EVFKATLKDG SSVAIKKLIR LSCQGDREFM AEMETLGKIK HRNLVPLLGY 900
    CKIGEERLLV YEFMQYGSLE EVLHGPRTGE KRRILGWEER KKIAKGAAKG 950
    LCFLHHNCIP HIIHRDMKSS NVLLDQDMEA RVSDFGMARL ISALDTHLSV 1000
    STLAGTPGYV PPEYYQSFRC TAKGDVYSIG VVMLEILSGK RPTDKEEFGD 1050
    TNLVGWSKMK AREGKHMEVI DEDLLKEGSS ESLNEKEGFE GGVIVKEMLR 1100
    YLEIALRCVD DFPSKRPNML QVVASLRELR GSENNSHSHS NSL 1143
    Length:1,143
    Mass (Da):125,676
    Last modified:May 1, 1999 - v1
    Checksum:i7D1C88493F27A94E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006532 Genomic DNA. Translation: AAD20088.1.
    CP002685 Genomic DNA. Translation: AEC05526.1.
    AY074313 mRNA. Translation: AAL67010.1.
    PIRiB84431.
    RefSeqiNP_178304.1. NM_126256.3.
    UniGeneiAt.13922.

    Genome annotation databases

    EnsemblPlantsiAT2G01950.1; AT2G01950.1; AT2G01950.
    GeneIDi814726.
    KEGGiath:AT2G01950.

    Cross-referencesi

    Web resourcesi

    PlantP kinase Classification PPC

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006532 Genomic DNA. Translation: AAD20088.1 .
    CP002685 Genomic DNA. Translation: AEC05526.1 .
    AY074313 mRNA. Translation: AAL67010.1 .
    PIRi B84431.
    RefSeqi NP_178304.1. NM_126256.3.
    UniGenei At.13922.

    3D structure databases

    ProteinModelPortali Q9ZPS9.
    SMRi Q9ZPS9. Positions 35-727, 790-1131.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9ZPS9. 64 interactions.
    MINTi MINT-8064047.

    Proteomic databases

    PaxDbi Q9ZPS9.
    PRIDEi Q9ZPS9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G01950.1 ; AT2G01950.1 ; AT2G01950 .
    GeneIDi 814726.
    KEGGi ath:AT2G01950.

    Organism-specific databases

    GeneFarmi 616. 54.
    TAIRi AT2G01950.

    Phylogenomic databases

    eggNOGi COG4886.
    HOGENOMi HOG000116551.
    InParanoidi Q9ZPS9.
    OMAi AASWANS.
    PhylomeDBi Q9ZPS9.

    Enzyme and pathway databases

    BioCyci ARA:AT2G01950-MONOMER.

    Gene expression databases

    ArrayExpressi Q9ZPS9.
    Genevestigatori Q9ZPS9.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR013320. ConA-like_subgrp.
    IPR011009. Kinase-like_dom.
    IPR001611. Leu-rich_rpt.
    IPR013210. LRR-contain_N2.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00560. LRR_1. 3 hits.
    PF13855. LRR_8. 2 hits.
    PF08263. LRRNT_2. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS51450. LRR. 17 hits.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "VH1, a provascular cell-specific receptor kinase that influences leaf cell patterns in Arabidopsis."
      Clay N.K., Nelson T.
      Plant Cell 14:2707-2722(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    5. "BRL1 and BRL3 are novel brassinosteroid receptors that function in vascular differentiation in Arabidopsis."
      Cano-Delgado A., Yin Y., Yu C., Vafeados D., Mora-Garcia S., Cheng J.-C., Nam K.H., Li J., Chory J.
      Development 131:5341-5351(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, LACK OF STEROID-BINDING.
    6. "VH1/BRL2 receptor-like kinase interacts with vascular-specific adaptor proteins VIT and VIK to influence leaf venation."
      Ceserani T., Trofka A., Gandotra N., Nelson T.
      Plant J. 57:1000-1014(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH TTL3, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiBRL2_ARATH
    AccessioniPrimary (citable) accession number: Q9ZPS9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 4, 2005
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3