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Protein

Serine/threonine-protein kinase BRI1-like 2

Gene

BRL2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor with a serine/threonine-protein kinase activity, which may transduce extracellular spatial and temporal signals into downstream cell differentiation responses in provascular and procambial cells. In contrast to BRI1, BRL1 and BRL3, it does not bind brassinolide.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei866 – 8661ATPPROSITE-ProRule annotation
Active sitei966 – 9661Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi844 – 8529ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. peptide receptor activity Source: GO_Central
  3. transmembrane receptor protein serine/threonine kinase activity Source: GO_Central
  4. ubiquitin protein ligase binding Source: GO_Central

GO - Biological processi

  1. auxin-activated signaling pathway Source: TAIR
  2. brassinosteroid mediated signaling pathway Source: TAIR
  3. leaf vascular tissue pattern formation Source: TAIR
  4. phloem transport Source: TAIR
  5. protein autophosphorylation Source: GO_Central
  6. transmembrane receptor protein serine/threonine kinase signaling pathway Source: GOC
  7. xylem and phloem pattern formation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G01950-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase BRI1-like 2 (EC:2.7.11.1)
Alternative name(s):
BRASSINOSTEROID INSENSITIVE 1-like protein 2
Protein VASCULAR HIGHWAY 1
Gene namesi
Name:BRL2
Synonyms:VH1
Ordered Locus Names:At2g01950
ORF Names:F14H20.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G01950.

Subcellular locationi

  1. Cell membrane 1 Publication; Single-pass type I membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini32 – 756725ExtracellularSequence AnalysisAdd
BLAST
Transmembranei757 – 77721HelicalSequence AnalysisAdd
BLAST
Topological domaini778 – 1143366CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: GO_Central
  3. plasmodesma Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence AnalysisAdd
BLAST
Chaini32 – 11431112Serine/threonine-protein kinase BRI1-like 2PRO_0000024309Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi84 – 841N-linked (GlcNAc...)Sequence Analysis
Glycosylationi118 – 1181N-linked (GlcNAc...)Sequence Analysis
Glycosylationi163 – 1631N-linked (GlcNAc...)Sequence Analysis
Glycosylationi188 – 1881N-linked (GlcNAc...)Sequence Analysis
Glycosylationi226 – 2261N-linked (GlcNAc...)Sequence Analysis
Glycosylationi234 – 2341N-linked (GlcNAc...)Sequence Analysis
Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence Analysis
Glycosylationi312 – 3121N-linked (GlcNAc...)Sequence Analysis
Glycosylationi412 – 4121N-linked (GlcNAc...)Sequence Analysis
Glycosylationi469 – 4691N-linked (GlcNAc...)Sequence Analysis
Glycosylationi506 – 5061N-linked (GlcNAc...)Sequence Analysis
Glycosylationi681 – 6811N-linked (GlcNAc...)Sequence Analysis
Modified residuei835 – 8351PhosphothreonineBy similarity
Modified residuei911 – 9111PhosphotyrosineBy similarity
Modified residuei1009 – 10091PhosphotyrosineBy similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9ZPS9.
PRIDEiQ9ZPS9.

Expressioni

Tissue specificityi

Expressed in provascular and procambial sites throughout plant development. Expressed throughout globe- to heart-staged embryos. Then, it is restricted to procambial cells by the late torpedo stage, and this pattern persists throughout the duration of embryo development. After germination, it is expressed not only in procambial cells throughout the plant but also in all lateral organ primordia before the onset of vascularization.2 Publications

Gene expression databases

ExpressionAtlasiQ9ZPS9. baseline and differential.
GenevestigatoriQ9ZPS9.

Interactioni

Subunit structurei

Interacts with TTL3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
F7A19.9Q9XI872EBI-2292728,EBI-2292778
TTL3Q9SIN12EBI-2292728,EBI-2292882

Protein-protein interaction databases

IntActiQ9ZPS9. 64 interactions.
MINTiMINT-8064047.

Structurei

3D structure databases

ProteinModelPortaliQ9ZPS9.
SMRiQ9ZPS9. Positions 35-727, 790-1131.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati79 – 10022LRR 1Add
BLAST
Repeati104 – 12623LRR 2Add
BLAST
Repeati128 – 15023LRR 3Add
BLAST
Repeati153 – 17523LRR 4Add
BLAST
Repeati178 – 20023LRR 5Add
BLAST
Repeati205 – 22824LRR 6Add
BLAST
Repeati229 – 25123LRR 7Add
BLAST
Repeati253 – 27523LRR 8Add
BLAST
Repeati278 – 29922LRR 9Add
BLAST
Repeati302 – 32322LRR 10Add
BLAST
Repeati327 – 34923LRR 11Add
BLAST
Repeati351 – 37222LRR 12Add
BLAST
Repeati376 – 39823LRR 13Add
BLAST
Repeati400 – 42223LRR 14Add
BLAST
Repeati424 – 44623LRR 15Add
BLAST
Repeati448 – 47023LRR 16Add
BLAST
Repeati472 – 49423LRR 17Add
BLAST
Repeati496 – 51823LRR 18Add
BLAST
Repeati520 – 54223LRR 19Add
BLAST
Repeati612 – 63423LRR 20Add
BLAST
Repeati636 – 65823LRR 21Add
BLAST
Repeati660 – 68223LRR 22Add
BLAST
Repeati684 – 70623LRR 23Add
BLAST
Domaini838 – 1129292Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi68 – 758Cys pair 1
Motifi720 – 7278Cys pair 2

Domaini

Contains two pairs of conservatively spaced Cys (Cys pair 1 and 2) possibly involved in forming some heterodimers.By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 23 LRR (leucine-rich) repeats.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG4886.
HOGENOMiHOG000116551.
InParanoidiQ9ZPS9.
OMAiAASWANS.
PhylomeDBiQ9ZPS9.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR001611. Leu-rich_rpt.
IPR013210. LRR-contain_N2.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00560. LRR_1. 3 hits.
PF13855. LRR_8. 2 hits.
PF08263. LRRNT_2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51450. LRR. 17 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ZPS9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTSPIRVRI RTRIQISFIF LLTHLSQSSS SDQSSLKTDS LSLLSFKTMI
60 70 80 90 100
QDDPNNILSN WSPRKSPCQF SGVTCLGGRV TEINLSGSGL SGIVSFNAFT
110 120 130 140 150
SLDSLSVLKL SENFFVLNST SLLLLPLTLT HLELSSSGLI GTLPENFFSK
160 170 180 190 200
YSNLISITLS YNNFTGKLPN DLFLSSKKLQ TLDLSYNNIT GPISGLTIPL
210 220 230 240 250
SSCVSMTYLD FSGNSISGYI SDSLINCTNL KSLNLSYNNF DGQIPKSFGE
260 270 280 290 300
LKLLQSLDLS HNRLTGWIPP EIGDTCRSLQ NLRLSYNNFT GVIPESLSSC
310 320 330 340 350
SWLQSLDLSN NNISGPFPNT ILRSFGSLQI LLLSNNLISG DFPTSISACK
360 370 380 390 400
SLRIADFSSN RFSGVIPPDL CPGAASLEEL RLPDNLVTGE IPPAISQCSE
410 420 430 440 450
LRTIDLSLNY LNGTIPPEIG NLQKLEQFIA WYNNIAGEIP PEIGKLQNLK
460 470 480 490 500
DLILNNNQLT GEIPPEFFNC SNIEWVSFTS NRLTGEVPKD FGILSRLAVL
510 520 530 540 550
QLGNNNFTGE IPPELGKCTT LVWLDLNTNH LTGEIPPRLG RQPGSKALSG
560 570 580 590 600
LLSGNTMAFV RNVGNSCKGV GGLVEFSGIR PERLLQIPSL KSCDFTRMYS
610 620 630 640 650
GPILSLFTRY QTIEYLDLSY NQLRGKIPDE IGEMIALQVL ELSHNQLSGE
660 670 680 690 700
IPFTIGQLKN LGVFDASDNR LQGQIPESFS NLSFLVQIDL SNNELTGPIP
710 720 730 740 750
QRGQLSTLPA TQYANNPGLC GVPLPECKNG NNQLPAGTEE GKRAKHGTRA
760 770 780 790 800
ASWANSIVLG VLISAASVCI LIVWAIAVRA RRRDADDAKM LHSLQAVNSA
810 820 830 840 850
TTWKIEKEKE PLSINVATFQ RQLRKLKFSQ LIEATNGFSA ASMIGHGGFG
860 870 880 890 900
EVFKATLKDG SSVAIKKLIR LSCQGDREFM AEMETLGKIK HRNLVPLLGY
910 920 930 940 950
CKIGEERLLV YEFMQYGSLE EVLHGPRTGE KRRILGWEER KKIAKGAAKG
960 970 980 990 1000
LCFLHHNCIP HIIHRDMKSS NVLLDQDMEA RVSDFGMARL ISALDTHLSV
1010 1020 1030 1040 1050
STLAGTPGYV PPEYYQSFRC TAKGDVYSIG VVMLEILSGK RPTDKEEFGD
1060 1070 1080 1090 1100
TNLVGWSKMK AREGKHMEVI DEDLLKEGSS ESLNEKEGFE GGVIVKEMLR
1110 1120 1130 1140
YLEIALRCVD DFPSKRPNML QVVASLRELR GSENNSHSHS NSL
Length:1,143
Mass (Da):125,676
Last modified:May 1, 1999 - v1
Checksum:i7D1C88493F27A94E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006532 Genomic DNA. Translation: AAD20088.1.
CP002685 Genomic DNA. Translation: AEC05526.1.
AY074313 mRNA. Translation: AAL67010.1.
PIRiB84431.
RefSeqiNP_178304.1. NM_126256.3.
UniGeneiAt.13922.

Genome annotation databases

EnsemblPlantsiAT2G01950.1; AT2G01950.1; AT2G01950.
GeneIDi814726.
KEGGiath:AT2G01950.

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006532 Genomic DNA. Translation: AAD20088.1.
CP002685 Genomic DNA. Translation: AEC05526.1.
AY074313 mRNA. Translation: AAL67010.1.
PIRiB84431.
RefSeqiNP_178304.1. NM_126256.3.
UniGeneiAt.13922.

3D structure databases

ProteinModelPortaliQ9ZPS9.
SMRiQ9ZPS9. Positions 35-727, 790-1131.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9ZPS9. 64 interactions.
MINTiMINT-8064047.

Proteomic databases

PaxDbiQ9ZPS9.
PRIDEiQ9ZPS9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G01950.1; AT2G01950.1; AT2G01950.
GeneIDi814726.
KEGGiath:AT2G01950.

Organism-specific databases

GeneFarmi616. 54.
TAIRiAT2G01950.

Phylogenomic databases

eggNOGiCOG4886.
HOGENOMiHOG000116551.
InParanoidiQ9ZPS9.
OMAiAASWANS.
PhylomeDBiQ9ZPS9.

Enzyme and pathway databases

BioCyciARA:AT2G01950-MONOMER.

Miscellaneous databases

PROiQ9ZPS9.

Gene expression databases

ExpressionAtlasiQ9ZPS9. baseline and differential.
GenevestigatoriQ9ZPS9.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR001611. Leu-rich_rpt.
IPR013210. LRR-contain_N2.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00560. LRR_1. 3 hits.
PF13855. LRR_8. 2 hits.
PF08263. LRRNT_2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51450. LRR. 17 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "VH1, a provascular cell-specific receptor kinase that influences leaf cell patterns in Arabidopsis."
    Clay N.K., Nelson T.
    Plant Cell 14:2707-2722(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  5. "BRL1 and BRL3 are novel brassinosteroid receptors that function in vascular differentiation in Arabidopsis."
    Cano-Delgado A., Yin Y., Yu C., Vafeados D., Mora-Garcia S., Cheng J.-C., Nam K.H., Li J., Chory J.
    Development 131:5341-5351(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, LACK OF STEROID-BINDING.
  6. "VH1/BRL2 receptor-like kinase interacts with vascular-specific adaptor proteins VIT and VIK to influence leaf venation."
    Ceserani T., Trofka A., Gandotra N., Nelson T.
    Plant J. 57:1000-1014(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TTL3, TISSUE SPECIFICITY.

Entry informationi

Entry nameiBRL2_ARATH
AccessioniPrimary (citable) accession number: Q9ZPS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: May 1, 1999
Last modified: April 29, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.