Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9ZPS3 (DCE4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate decarboxylase 4

Short name=GAD 4
EC=4.1.1.15
Gene names
Name:GAD4
Ordered Locus Names:At2g02010
ORF Names:F14H20.8
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length493 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the production of GABA. The calmodulin-binding is calcium-dependent and it is proposed that this may, directly or indirectly, form a calcium regulated control of GABA biosynthesis By similarity.

Catalytic activity

L-glutamate = 4-aminobutanoate + CO2.

Cofactor

Pyridoxal phosphate By similarity.

Subunit structure

Homohexamer. Interacts with clamodulin By similarity.

Tissue specificity

Expressed in flowers and shoots. Detected at low levels in siliques, stems, leaves and roots. Ref.6

Induction

Up-regulated by salt treatment and hypoxia. Ref.6 Ref.7

Sequence similarities

Belongs to the group II decarboxylase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 493493Glutamate decarboxylase 4
PRO_0000416955

Amino acid modifications

Modified residue81Phosphoserine
Modified residue2771N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9ZPS3 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 36B3FC93F2978168

FASTA49356,005
        10         20         30         40         50         60 
MVLSKTVSES DVSIHSTFAS RYVRNSLPRF EMPENSIPKE AAYQIINDEL MLDGNPRLNL 

        70         80         90        100        110        120 
ASFVTTWMEP ECDKLMMESI NKNYVDMDEY PVTTELQNRC VNMIARLFNA PLGDGEAAVG 

       130        140        150        160        170        180 
VGTVGSSEAI MLAGLAFKRQ WQNKRKAQGL PYDKPNIVTG ANVQVCWEKF ARYFEVELKE 

       190        200        210        220        230        240 
VNLREDYYVM DPVKAVEMVD ENTICVAAIL GSTLTGEFED VKLLNDLLVE KNKQTGWDTP 

       250        260        270        280        290        300 
IHVDAASGGF IAPFLYPELE WDFRLPLVKS INVSGHKYGL VYAGIGWVVW RTKTDLPDEL 

       310        320        330        340        350        360 
IFHINYLGAD QPTFTLNFSK GSSQVIAQYY QLIRLGFEGY RNVMDNCREN MMVLRQGLEK 

       370        380        390        400        410        420 
TGRFKIVSKE NGVPLVAFSL KDSSRHNEFE VAHTLRRFGW IVPAYTMPAD AQHVTVLRVV 

       430        440        450        460        470        480 
IREDFSRTLA ERLVADFEKV LHELDTLPAR VHAKMANGKV NGVKKTPEET QREVTAYWKK 

       490 
LLETKKTNKN TIC 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Metabolism and functions of gamma-aminobutyric acid."
Shelp B.J., Bown A.W., McLean M.D.
Trends Plant Sci. 4:446-452(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
[5]"Site-specific phosphorylation profiling of Arabidopsis proteins by mass spectrometry and peptide chip analysis."
de la Fuente van Bentem S., Anrather D., Dohnal I., Roitinger E., Csaszar E., Joore J., Buijnink J., Carreri A., Forzani C., Lorkovic Z.J., Barta A., Lecourieux D., Verhounig A., Jonak C., Hirt H.
J. Proteome Res. 7:2458-2470(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-8, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Root.
[6]"Contribution of the GABA shunt to hypoxia-induced alanine accumulation in roots of Arabidopsis thaliana."
Miyashita Y., Good A.G.
Plant Cell Physiol. 49:92-102(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION BY HYPOXIA.
[7]"The Arabidopsis pop2-1 mutant reveals the involvement of GABA transaminase in salt stress tolerance."
Renault H., Roussel V., El Amrani A., Arzel M., Renault D., Bouchereau A., Deleu C.
BMC Plant Biol. 10:20-20(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY SALT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC006532 Genomic DNA. Translation: AAD20099.1.
CP002685 Genomic DNA. Translation: AEC05532.1.
AF361836 mRNA. Translation: AAK32848.1.
AY124860 mRNA. Translation: AAM70569.1.
PIRH84431.
RefSeqNP_178310.1. NM_126262.1.
UniGeneAt.28718.

3D structure databases

ProteinModelPortalQ9ZPS3.
SMRQ9ZPS3. Positions 12-448.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT2G02010.1-P.

Proteomic databases

PaxDbQ9ZPS3.
PRIDEQ9ZPS3.

Protocols and materials databases

DNASU814732.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G02010.1; AT2G02010.1; AT2G02010.
GeneID814732.
KEGGath:AT2G02010.

Organism-specific databases

TAIRAT2G02010.

Phylogenomic databases

eggNOGCOG0076.
HOGENOMHOG000070228.
InParanoidQ9ZPS3.
KOK01580.
OMACTEPHPA.
PhylomeDBQ9ZPS3.
ProtClustDBCLSN2683665.

Enzyme and pathway databases

BioCycARA:AT2G02010-MONOMER.

Gene expression databases

GenevestigatorQ9ZPS3.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
InterProIPR010107. Glutamate_decarboxylase.
IPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERPTHR11999:SF1. PTHR11999:SF1. 1 hit.
PfamPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMSSF53383. SSF53383. 1 hit.
TIGRFAMsTIGR01788. Glu-decarb-GAD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDCE4_ARATH
AccessionPrimary (citable) accession number: Q9ZPS3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: May 1, 1999
Last modified: March 19, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names