Q9ZPP1 (CALR_BERST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Calreticulin
Organism	Berberis stolonifera (Barberry)
Taxonomic identifier	33814 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › stem eudicotyledons › Ranunculales › Berberidaceae › Berberis

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER By similarity.
Subcellular location	Endoplasmic reticulum lumen By similarity.
Domain	Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity By similarity. The interaction with glycans occurs through a binding site in the globular lectin domain By similarity. The zinc binding sites are localized to the N-domain By similarity.
Sequence similarities	Belongs to the calreticulin family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Repeat Signal
Ligand	Calcium Lectin Metal-binding Zinc
Molecular function	Chaperone
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological_process	protein folding Inferred from electronic annotation. Source: InterPro
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – ?		Potential
	Chain	? – 416		Calreticulin		PRO_0000004187
Regions
	Repeat	194 – 205	12	1-1
	Repeat	213 – 224	12	1-2
	Repeat	230 – 241	12	1-3
	Repeat	248 – 259	12	1-4
	Repeat	263 – 273	11	2-1
	Repeat	277 – 287	11	2-2
	Repeat	291 – 301	11	2-3
	Region	194 – 259	66	4 X approximate repeats
	Region	263 – 301	39	3 X approximate repeats
	Motif	413 – 416	4	Prevents secretion from ER Potential
	Compositional bias	355 – 411	57	Asp/Glu/Lys-rich
Sites
	Binding site	112	1	Carbohydrate By similarity
	Binding site	114	1	Carbohydrate By similarity
	Binding site	131	1	Carbohydrate By similarity
	Binding site	138	1	Carbohydrate By similarity
	Binding site	321	1	Carbohydrate By similarity
Amino acid modifications
	Glycosylation	54	1	N-linked (GlcNAc...) Potential
	Disulfide bond	108 ↔ 140		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZPP1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 08AC46885BF69114	FASTA	416	47,927
	10 20 30 40 50 60 MAIAERRSRS HLALRVRDRV SAEVFFEERF EDGWESKWVK SDWKRDENMA GEWNFTSGKW 70 80 90 100 110 120 NGDANDKGIQ TSEDYRFYAI SAAFPEFSNK GKTLVFQFSV KHEQKLDCGG GYMKLLSGDV 130 140 150 160 170 180 DQKKFGGDTP YSIMFGPDIC GYSTKKVHAI LTKGETNHLI KKDVPCETDQ LTHVYTFILR 190 200 210 220 230 240 PDASYSILID NVEKQSGSVY TDWDILPPKQ IKDPEAKKPE DWEDKEYIPD PEDKKPEGYD 250 260 270 280 290 300 DIPKEITDPE AKKPEDWDDE EDGEWTAPTI PNPDYKGEWK PKKIKNPNFK GKWKAPMIDN 310 320 330 340 350 360 PDFKDDPDIY VFPKLKYVGI ELWQVKSGTM FDNVLICDDP DYAKKLAEET WGKNKDAEKA 370 380 390 400 410 AFDEAEKKKE EEEAKDDPTE SDDEKPDEEG ESDGEGDDES KDIDNEEDDE DVHDEL

References

[1]	"Calreticulin from Berberis stolonifera." Chou W.-M., Kutchan T.M. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF052040 mRNA. Translation: AAD17490.1.
3D structure databases
ProteinModelPortal	Q9ZPP1.
SMR	Q9ZPP1. Positions 209-282.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.10.250.10. 1 hit. 2.60.120.200. 1 hit.
InterPro	IPR001580. Calret/calnex. IPR018124. Calret/calnex_CS. IPR009169. Calreticulin. IPR009033. Calreticulin/calnexin_P_dom. IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. [Graphical view]
PANTHER	PTHR11073. PTHR11073. 1 hit.
Pfam	PF00262. Calreticulin. 1 hit. [Graphical view]
PIRSF	PIRSF002356. Calreticulin. 1 hit.
PRINTS	PR00626. CALRETICULIN.
SUPFAM	SSF63887. Calret_calnex_P. 1 hit. SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS00803. CALRETICULIN_1. 1 hit. PS00804. CALRETICULIN_2. 1 hit. PS00805. CALRETICULIN_REPEAT. 2 hits. PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CALR_BERST

Accession

Primary (citable) accession number: Q9ZPP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1999
Last modified:	March 6, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program