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Protein
Submitted name:

Cystathionine gamma-synthase

Gene

metB

Organism
Nicotiana tabacum (Common tobacco)
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Experimental evidence at protein leveli

Functioni

Cofactori

pyridoxal 5'-phosphateUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei261 – 2611N6-(pyridoxalphosphate)lysine (covalent)Combined sources

GO - Molecular functioni

  1. catalytic activity Source: InterPro
  2. pyridoxal phosphate binding Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Pyridoxal phosphateUniRule annotation

Enzyme and pathway databases

SABIO-RKQ9ZPL5.

Names & Taxonomyi

Protein namesi
Submitted name:
Cystathionine gamma-synthaseImported
Gene namesi
Name:metBImported
OrganismiNicotiana tabacum (Common tobacco)Imported
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1I41X-ray3.20A/B/C/D/E/F/G/H/I/J/K/L1-445[»]
1I43X-ray3.10A/B/C/D/E/F/G/H/I/J/K/L1-445[»]
1I48X-ray3.25A/B/C/D/E/F/G/H/I/J/K/L1-445[»]
1QGNX-ray2.90A/B/C/D/E/F/G/H1-445[»]
ProteinModelPortaliQ9ZPL5.
SMRiQ9ZPL5. Positions 49-445.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9ZPL5.

Family & Domainsi

Sequence similaritiesi

Belongs to the trans-sulfuration enzymes family.UniRule annotation

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 1 hit.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
PIRSFiPIRSF001434. CGS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZPL5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKAVDAAAA AAAAIAPVDT TVVNEDVALV ENETCNDQNV QFDSLPSMKY
60 70 80 90 100
ASFLNSDGSV AIHAGERLGR GIVTDAITTP VVNTSAYFFN KTSELIDFKE
110 120 130 140 150
KRRASFEYGR YGNPTTVVLE EKISALEGAE STLLMASGMC ASTVMLLALV
160 170 180 190 200
PAGGHIVTTT DCYRKTRIFI ETILPKMGIT ATVIDPADVG ALELALNQKK
210 220 230 240 250
VNLFFTESPT NPFLRCVDIE LVSKLCHEKG ALVCIDGTFA TPLNQKALAL
260 270 280 290 300
GADLVLHSAT KFLGGHNDVL AGCISGPLKL VSEIRNLHHI LGGALNPNAA
310 320 330 340 350
YLIIRGMKTL HLRVQQQNST ALRMAEILEA HPKVRHVYYP GLQSHPEHHI
360 370 380 390 400
AKKQMTGFGG AVSFEVDGDL LTTAKFVDAL KIPYIAPSFG GCESIVDQPA
410 420 430 440
IMSYWDLSQS DRAKYGIMDN LVRFSFGVED FDDLKADILQ ALDSI
Length:445
Mass (Da):48,017
Last modified:May 1, 1999 - v1
Checksum:iD2936EDA41F02D44
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF097180 mRNA. Translation: AAD16143.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF097180 mRNA. Translation: AAD16143.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1I41X-ray3.20A/B/C/D/E/F/G/H/I/J/K/L1-445[»]
1I43X-ray3.10A/B/C/D/E/F/G/H/I/J/K/L1-445[»]
1I48X-ray3.25A/B/C/D/E/F/G/H/I/J/K/L1-445[»]
1QGNX-ray2.90A/B/C/D/E/F/G/H1-445[»]
ProteinModelPortaliQ9ZPL5.
SMRiQ9ZPL5. Positions 49-445.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKQ9ZPL5.

Miscellaneous databases

EvolutionaryTraceiQ9ZPL5.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 1 hit.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
PIRSFiPIRSF001434. CGS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning, purification and characterization of cystathionine gamma-synthase from Nicotiana tabacum."
    Clausen T., Laber B., Streber W.
    Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "The crystal structure of cystathionine gamma-synthase from Nicotiana tabacum reveals its substrate and reaction specificity."
    Steegborn C., Messerschmidt A., Laber B., Streber W., Huber R., Clausen T.
    J. Mol. Biol. 290:983-996(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.90 ANGSTROMS) IN COMPLEX WITH N6-(PYRIDOXALPHOSPHATE)LYSINE, ACTIVE SITE.
  3. "Crystal structures of cystathionine gamma-synthase inhibitor complexes rationalize the increased affinity of a novel inhibitor."
    Steegborn C., Laber B., Messerschmidt A., Huber R., Clausen T.
    J. Mol. Biol. 311:789-801(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.10 ANGSTROMS) IN COMPLEX WITH N6-(PYRIDOXALPHOSPHATE)LYSINE.

Entry informationi

Entry nameiQ9ZPL5_TOBAC
AccessioniPrimary (citable) accession number: Q9ZPL5
Entry historyi
Integrated into UniProtKB/TrEMBL: May 1, 1999
Last sequence update: May 1, 1999
Last modified: January 7, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.