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Q9ZPI6

- AIM1_ARATH

UniProt

Q9ZPI6 - AIM1_ARATH

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Protein

Peroxisomal fatty acid beta-oxidation multifunctional protein AIM1

Gene

AIM1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in peroxisomal fatty acid beta-oxidation. Required for wound-induced jasmonate biosynthesis. Possesses enoyl-CoA hydratase activity against short chain substrates (C4-C6) and 3-hydroxyacyl-CoA dehydrogenase activity against chains of variable sizes (C6-C16).3 Publications

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.
(3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.

Kineticsi

  1. KM=115 µM for crotonyl-CoA1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei116 – 1161NucleophileSequence Analysis
Active sitei136 – 1361Proton acceptorSequence Analysis

GO - Molecular functioni

  1. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB
  2. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB-EC
  3. coenzyme binding Source: InterPro
  4. dodecenoyl-CoA delta-isomerase activity Source: UniProtKB-EC
  5. enoyl-CoA hydratase activity Source: UniProtKB

GO - Biological processi

  1. fatty acid beta-oxidation Source: TAIR
  2. flower development Source: TAIR
  3. jasmonic acid biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase, Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciMetaCyc:AT4G29010-MONOMER.
UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal fatty acid beta-oxidation multifunctional protein AIM1
Alternative name(s):
Protein ABNORMAL INFLORESCENCE MERISTEM 1
Short name:
AtAIM1
Including the following 2 domains:
Enoyl-CoA hydratase/3-2-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase (EC:4.2.1.17, EC:5.1.2.3, EC:5.3.3.8)
3-hydroxyacyl-CoA dehydrogenase (EC:1.1.1.35)
Gene namesi
Name:AIM1
Ordered Locus Names:At4g29010
ORF Names:F19B15.40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G29010.

Subcellular locationi

Peroxisome Curated

GO - Cellular componenti

  1. cell wall Source: TAIR
  2. chloroplast Source: TAIR
  3. peroxisome Source: TAIR
  4. plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Disruption phenotypei

Reduced rosette size, twisted leaves, and abnormal and sterile flowers.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 721721Peroxisomal fatty acid beta-oxidation multifunctional protein AIM1PRO_0000401372Add
BLAST

Proteomic databases

PaxDbiQ9ZPI6.
PRIDEiQ9ZPI6.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

ExpressionAtlasiQ9ZPI6. baseline and differential.
GenevestigatoriQ9ZPI6.

Interactioni

Protein-protein interaction databases

BioGridi14309. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9ZPI6.
SMRiQ9ZPI6. Positions 9-712.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The epimerase and isomerase activities are contained in the N-terminal region while the dehydrogenase activity is in the C-terminal region.By similarity

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.Curated
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.Curated

Phylogenomic databases

eggNOGiCOG1250.
HOGENOMiHOG000261347.
InParanoidiQ9ZPI6.
KOiK10527.
OMAiICLADAG.
PhylomeDBiQ9ZPI6.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR018376. Enoyl-CoA_hyd/isom_CS.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 1 hit.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
PROSITEiPS00067. 3HCDH. 1 hit.
PS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZPI6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKKIGVTME VGNDGVAVIT ISNPPVNSLA SPIISGLKEK FRDANQRNDV
60 70 80 90 100
KAIVLIGNNG RFSGGFDINV FQQVHKTGDL SLMPEVSVEL VCNLMEDSRK
110 120 130 140 150
PVVAAVEGLA LGGGLELAMA CHARVAAPKA QLGLPELTLG VIPGFGGTQR
160 170 180 190 200
LPRLVGLAKA TDMILLSKSI SSEEGHKLGL IDALVPPGDV LSTSRKWALD
210 220 230 240 250
IAEGRKPFLQ SLHRTDKIGS LSEARAILKN SRQLAKKIAP NMPQHHACIE
260 270 280 290 300
VIEEGIIHGG YSGVLKEAEV FKQLVLSDTA KGLVHVFFAQ RATSKVPNVT
310 320 330 340 350
DVGLKPRPIK KVAVIGGGLM GSGIATALLL SNIRVVLKEI NSEFLMKGIK
360 370 380 390 400
SVEANMKSLV SRGKLTQDKA GKALSLFKGV LDYTEFNDVD MVIEAVIENI
410 420 430 440 450
QLKQNIFKEI EKVCSPHCIL ASNTSTIDLD VIGEKTNSKD RIVGAHFFSP
460 470 480 490 500
AHLMPLLEIV RSKNTSAQVI LDLMAVGKAI KKVPVVVGNC IGFAVNRTFF
510 520 530 540 550
PYSQAAHMLA NLGVDLFRID SVITSFGLPL GPFQLGDLAG HGIGLAVGPI
560 570 580 590 600
YAKVYGDRMF RSPMTELLLK SGRNGKINGR GYYIYEKGSK PKPDPSVLSI
610 620 630 640 650
VEKSRKLTNI MPGGKPISVT DKEIVEMILF PVVNEACRVL DEGVVIRASD
660 670 680 690 700
LDIASVLGMS FPSYRGGIVF WADTVGPKYI YERLKKLSET YGSFFKPSRY
710 720
LEERAMNGML LSESKSSRSK L
Length:721
Mass (Da):77,858
Last modified:May 1, 1999 - v1
Checksum:iF72FB3252B2216CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF123253 Genomic DNA. Translation: AAD18041.1.
AL078470 Genomic DNA. Translation: CAB43915.1.
AL161574 Genomic DNA. Translation: CAB79659.1.
CP002687 Genomic DNA. Translation: AEE85572.1.
AY059815 mRNA. Translation: AAL24297.1.
AY072072 mRNA. Translation: AAL59895.1.
AY096659 mRNA. Translation: AAM20293.1.
PIRiT08956.
RefSeqiNP_194630.1. NM_119045.4.
UniGeneiAt.3404.
At.48915.

Genome annotation databases

EnsemblPlantsiAT4G29010.1; AT4G29010.1; AT4G29010.
GeneIDi829022.
KEGGiath:AT4G29010.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF123253 Genomic DNA. Translation: AAD18041.1 .
AL078470 Genomic DNA. Translation: CAB43915.1 .
AL161574 Genomic DNA. Translation: CAB79659.1 .
CP002687 Genomic DNA. Translation: AEE85572.1 .
AY059815 mRNA. Translation: AAL24297.1 .
AY072072 mRNA. Translation: AAL59895.1 .
AY096659 mRNA. Translation: AAM20293.1 .
PIRi T08956.
RefSeqi NP_194630.1. NM_119045.4.
UniGenei At.3404.
At.48915.

3D structure databases

ProteinModelPortali Q9ZPI6.
SMRi Q9ZPI6. Positions 9-712.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 14309. 1 interaction.

Proteomic databases

PaxDbi Q9ZPI6.
PRIDEi Q9ZPI6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G29010.1 ; AT4G29010.1 ; AT4G29010 .
GeneIDi 829022.
KEGGi ath:AT4G29010.

Organism-specific databases

TAIRi AT4G29010.

Phylogenomic databases

eggNOGi COG1250.
HOGENOMi HOG000261347.
InParanoidi Q9ZPI6.
KOi K10527.
OMAi ICLADAG.
PhylomeDBi Q9ZPI6.

Enzyme and pathway databases

UniPathwayi UPA00659 .
BioCyci MetaCyc:AT4G29010-MONOMER.

Gene expression databases

ExpressionAtlasi Q9ZPI6. baseline and differential.
Genevestigatori Q9ZPI6.

Family and domain databases

Gene3Di 1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
InterProi IPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR018376. Enoyl-CoA_hyd/isom_CS.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00725. 3HCDH. 1 hit.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view ]
SUPFAMi SSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
PROSITEi PS00067. 3HCDH. 1 hit.
PS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A defect in beta-oxidation causes abnormal inflorescence development in Arabidopsis."
    Richmond T.A., Bleecker A.B.
    Plant Cell 11:1911-1924(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Jasmonate biosynthesis in Arabidopsis thaliana requires peroxisomal beta-oxidation enzymes--additional proof by properties of pex6 and aim1."
    Delker C., Zolman B.K., Miersch O., Wasternack C.
    Phytochemistry 68:1642-1650(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "The multifunctional protein in peroxisomal beta-oxidation: structure and substrate specificity of the Arabidopsis thaliana protein MFP2."
    Arent S., Christensen C.E., Pye V.E., Noergaard A., Henriksen A.
    J. Biol. Chem. 285:24066-24077(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiAIM1_ARATH
AccessioniPrimary (citable) accession number: Q9ZPI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: May 1, 1999
Last modified: October 29, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3