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Protein

Arginase 2, chloroplastic/mitochondrial

Gene

ARGAH2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of L-arginine to urea and L-ornithine. The latter can be utilized in the urea cycle or as a precursor for the synthesis of both polyamines and proline (By similarity). Possesses agmatinase activity. Catalyzes the formation of putrescine from agmatine (PubMed:28716421).By similarity1 Publication

Miscellaneous

Plants over-expressing ARGAH2 have decreased susceptibility to the fungal pathogen B.cinerea.1 Publication

Catalytic activityi

L-arginine + H2O = L-ornithine + urea.By similarity
Agmatine + H2O = putrescine + urea.1 Publication

Cofactori

Mn2+PROSITE-ProRule annotationNote: Binds 2 manganese ions per subunit.PROSITE-ProRule annotation

Kineticsi

  1. KM=72.6 µM for agmatine1 Publication

    Pathwayi: urea cycle

    This protein is involved in step 1 of the subpathway that synthesizes L-ornithine and urea from L-arginine.By similarity
    Proteins known to be involved in this subpathway in this organism are:
    1. Arginase 1, mitochondrial (ARGAH1), Arginase 2, chloroplastic/mitochondrial (ARGAH2)
    This subpathway is part of the pathway urea cycle, which is itself part of Nitrogen metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-ornithine and urea from L-arginine, the pathway urea cycle and in Nitrogen metabolism.

    Pathwayi: putrescine biosynthesis via agmatine pathway

    This protein is involved in step 1 of the subpathway that synthesizes putrescine from agmatine.1 Publication
    Proteins known to be involved in this subpathway in this organism are:
    1. Arginase 1, mitochondrial (ARGAH1), Arginase 2, chloroplastic/mitochondrial (ARGAH2)
    This subpathway is part of the pathway putrescine biosynthesis via agmatine pathway, which is itself part of Amine and polyamine biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes putrescine from agmatine, the pathway putrescine biosynthesis via agmatine pathway and in Amine and polyamine biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi163Manganese 1PROSITE-ProRule annotation1
    Metal bindingi187Manganese 1PROSITE-ProRule annotation1
    Metal bindingi187Manganese 2PROSITE-ProRule annotation1
    Metal bindingi189Manganese 2PROSITE-ProRule annotation1
    Metal bindingi191Manganese 1PROSITE-ProRule annotation1
    Binding sitei228SubstrateBy similarity1
    Metal bindingi272Manganese 1PROSITE-ProRule annotation1
    Metal bindingi272Manganese 2PROSITE-ProRule annotation1
    Metal bindingi274Manganese 2PROSITE-ProRule annotation1
    Binding sitei315SubstrateBy similarity1

    GO - Molecular functioni

    • agmatinase activity Source: TAIR
    • arginase activity Source: TAIR
    • cobalt ion binding Source: TAIR
    • manganese ion binding Source: EnsemblPlants

    GO - Biological processi

    • arginine catabolic process Source: EnsemblPlants
    • defense response to fungus Source: TAIR
    • ornithine metabolic process Source: TAIR
    • proline metabolic process Source: TAIR
    • putrescine biosynthetic process Source: TAIR
    • putrescine biosynthetic process from arginine, using agmatinase Source: UniProtKB
    • putrescine metabolic process Source: TAIR
    • tyrosine metabolic process Source: TAIR
    • urea cycle Source: UniProtKB-UniPathway

    Keywordsi

    Molecular functionHydrolase
    Biological processArginine metabolism, Putrescine biosynthesis
    LigandManganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiR-ATH-351143 Agmatine biosynthesis
    UniPathwayiUPA00158; UER00270
    UPA00534; UER00287

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arginase 2, chloroplastic/mitochondrial (EC:3.5.3.1By similarity)
    Alternative name(s):
    Agmatinase ARGAH2Curated (EC:3.5.3.111 Publication)
    Arginine amidohydrolase 2
    Gene namesi
    Name:ARGAH2
    Ordered Locus Names:At4g08870
    ORF Names:T3H13.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 4

    Organism-specific databases

    AraportiAT4G08870
    TAIRilocus:2138743 AT4G08870

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Chloroplast, Mitochondrion, Plastid

    Pathology & Biotechi

    Disruption phenotypei

    Increased formation of lateral and adventitious roots and increased production of NO in roots.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 26Chloroplast and mitochondrionSequence analysisAdd BLAST26
    ChainiPRO_000017370427 – 344Arginase 2, chloroplastic/mitochondrialAdd BLAST318

    Proteomic databases

    PaxDbiQ9ZPF5
    PRIDEiQ9ZPF5

    2D gel databases

    SWISS-2DPAGEiQ9ZPF5

    Expressioni

    Tissue specificityi

    Expressed in vasculature of roots, root tips, leaves and cotyledons.2 Publications

    Inductioni

    By methyl jasmonate and infection with the fungal pathogen B.cinerea.2 Publications

    Gene expression databases

    ExpressionAtlasiQ9ZPF5 baseline and differential
    GenevisibleiQ9ZPF5 AT

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT4G08870.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ZPF5
    SMRiQ9ZPF5
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni189 – 193Substrate bindingBy similarity5
    Regioni197 – 199Substrate bindingBy similarity3

    Sequence similaritiesi

    Belongs to the arginase family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiKOG2964 Eukaryota
    COG0010 LUCA
    HOGENOMiHOG000204320
    InParanoidiQ9ZPF5
    KOiK01476
    OMAiSWCTHIL
    OrthoDBiEOG09360FUJ
    PhylomeDBiQ9ZPF5

    Family and domain databases

    InterProiView protein in InterPro
    IPR006035 Ureohydrolase
    IPR023696 Ureohydrolase_dom_sf
    PfamiView protein in Pfam
    PF00491 Arginase, 1 hit
    PIRSFiPIRSF036979 Arginase, 1 hit
    SUPFAMiSSF52768 SSF52768, 1 hit
    PROSITEiView protein in PROSITE
    PS51409 ARGINASE_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

    Note: A number of isoforms are produced. According to EST sequences.
    Isoform 1 (identifier: Q9ZPF5-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MWKIGQRGVP YFQRLIAAPF TTLRSLPTSL VETGQNRVID ASLTLIRERA
    60 70 80 90 100
    KLKGELVRLI GGAKATTALL GVPLGHNSSF LEGPALAPPH VREAIWCGST
    110 120 130 140 150
    NSTTEEGKEL KDPRVLSDVG DIPVQEIREM GVDDDRLMKV VSESVKLVME
    160 170 180 190 200
    EEPLRPLVIG GDHSISYPVV RAVSEKLGGP VDILHLDAHP DIYDRFEGNY
    210 220 230 240 250
    YSHASSFARI MEGGYARRLL QVGIRSINKE GREQGKRFGV EQYEMRTFSK
    260 270 280 290 300
    DRQMLENLKL GEGVKGVYIS IDVDCLDPGF AHGVSHFEPG GLSFRDVLNI
    310 320 330 340
    LHNLQGDLVG ADVVEYNPQR DTADDMTAMV AAKFVRELAA KMSK
    Length:344
    Mass (Da):37,980
    Last modified:May 1, 1999 - v1
    Checksum:iFED95D31B1A2FEBE
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF128396 Genomic DNA Translation: AAD17371.1
    AL161513 Genomic DNA Translation: CAB78011.1
    CP002687 Genomic DNA Translation: AEE82684.1
    BT003815 mRNA Translation: AAO41868.1
    PIRiC85089
    RefSeqiNP_192626.1, NM_116956.4 [Q9ZPF5-1]
    UniGeneiAt.4188

    Genome annotation databases

    EnsemblPlantsiAT4G08870.1; AT4G08870.1; AT4G08870 [Q9ZPF5-1]
    GeneIDi826458
    GrameneiAT4G08870.1; AT4G08870.1; AT4G08870 [Q9ZPF5-1]
    KEGGiath:AT4G08870

    Keywords - Coding sequence diversityi

    Alternative splicing

    Similar proteinsi

    Entry informationi

    Entry nameiARGI2_ARATH
    AccessioniPrimary (citable) accession number: Q9ZPF5
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: May 1, 1999
    Last modified: April 25, 2018
    This is version 139 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

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